Md Irshad

Intrinsic viscosities [η] for 0.2, 0.4, 0.6, and 0.8 mg% aqueous of each Bovine serum albumin
(BSA) and egg albumin (EA) solutions under zero, 0.5 and 0.7 magnetic flux at 298.15 K and
1 atmosphere are reported. The [η] data for BSA are higher than EA and values of both
increase with magnetic flux strengthening molecular interactions.

Doses of 0.0150 weber m− 2 magnetic flux for 30 minutes are given to four series of
biomolecules in aqueous medium, densities (ρ), viscosities (η), and surface tension (γ) were
measured at 304.15 K. The vitamins and carbohydrates decrease densities and viscosities
and the difference of density from magnet to aqueous solution in order of dextrose (−
0.00025 kg m− 3)> fructose (− 0.00021 kg m− 3)= B6 (− 0.00021 kg m− 3)> starch (−
0.00018 kg m− 3)> B1 (− 0.0018 kg m− 3), while viscosity in order of starch (− 0.0044 kg m ...

Cassia fistula oil was investigated for antifungal activities against standard and clinical isolates of Candida species. Gas chromatography coupled with mass
spectrometric (GC-MS) analysis of C. fistula oil revealed the presence of antimicrobial compounds like β-sitosterol, stigmasterol, ergosterol, betulinic acid,
lupeol, fucosterol, α-amyrin and friedelin. The minimum inhibitory concentration (MIC) of the pulp and seed oils ranged between 250-300 and 350-500 μg/mL
respectively. Both oils also inhibited by ≥63.8% ergosterol bio-synthesis in Candida cell wall {fluconazole (standard) ≥89.1%)}. The MICs were significantly
correlated with the ergosterol content decrease in the cell wall (Student’s t test p ≤ 0.005). We can, therefore, conclude that active compounds are present in
Cassia fistula oil that primarily target ergosterol biosynthesis in Candida cell wall.

Cassia fistula Linn. (Fabaceae) fruit has been used in folk medicine and reported for various pharmacological properties. We evaluated the anticancer activity of C. fistula fruit extracts [ethyl acetate extract of pulp (EEP), n-butanol extract of pulp (BEP), ethyl acetate extract of seeds (EES), n-butanol extract of seeds (BES)] against human cervical cancer (SiHa) and breast cancer (MCF-7) cell lines. Phytochemicals analysis revealed that the EEP and EES contained anticancer compounds like 2(3H)-furanone, rhein, thymoland oleic acid. BES contained inositol and palmitic acid, whereas BEP contained inositol and 2-pyrrolidone. IC50 of EEP, EES, BEP and BES against SiHa cells were 415.5±0.19, 435.7±0.11, 535.3±0.32 and 580.2±0.41 μg/mL, whereas against MCF-7 cells were 422.2±0.32, 51.4±0.27, 564.5±0.21 and 575.6±0.47 μg/mL respectively (p < 0.01). At IC50, EEP, EES, BEP and BES treated MCF-7 cells formed colonies of 17.5, 16.3, 22.5 and 18.8 % whereas treated SiHa cells formed colonies of 11.5, 12.8, 19.2 and 16.7 % (p < 0.03). Seeds and pulp treated both cell lines showed up-regulation of p53 and Bax genes, down-regulation of Bcl-2 gene and increased
caspase-3, 7 & 10 and -9 enzymes activities. Also, the fragmented genomic DNA in pulp and seeds extracts treated SiHa and MCF-7 cells showed sign of apoptosis.We concluded that the fruit pulp and seeds extracts inhibited MCF-7 and SiHa cells growth and induced cells death by modulating the expression of apoptosisregulatory genes and caspase enzymes.