Research Interests: Free Radicals, Flow Cytometry, Bone Metabolism, Cell Biology, Apoptosis, and 25 moreMacromolecular X-Ray Crystallography, Free Radical, Mesenchymal stem cells, Blood Glucose, Diabetes mellitus, Trabecular Bone, Tibia, Animals, Male, Polymerase Chain Reaction, Mesenchymal Stem Cell, Cardio vascular disease, Medical Physiology, Alkaline phosphatase, Rats, Bone Loss, Computerized tomography, Body Weight, Bone Formation, Streptozotocin, Experimental Diabetes Mellitus Type 1 and 2, Diabetic Rat, Cell Survival, Mesenchymal Stromal Cells, and Biochemistry and cell biology
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The discovery of the unique features of stem cells and induction of pluripotency in somatic cells revealed promising new strategies for therapies of degenerative diseases, disease modeling or drug discovery. Stem cell cultivation still... more
The discovery of the unique features of stem cells and induction of pluripotency in somatic cells revealed promising new strategies for therapies of degenerative diseases, disease modeling or drug discovery. Stem cell cultivation still depends on the use of feeder cells. The application of the CellCelector™ to pick stem cell colonies or colony fractions provides a highly precise alternative to manual picking procedures.
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Cells are highly active, living objects. Under the microscope just a fixed snap-shot of the cell culture can be visualized. By observation in real-time complex interactions, migration or slowly morphological changes remain invisible.... more
Cells are highly active, living objects. Under the microscope just a fixed snap-shot of the cell culture can be visualized. By observation in real-time complex interactions, migration or slowly morphological changes remain invisible. Additional to picking various cell types the CellCelector™ is suitable for exposure of time lapse series to provide an insight into activity, proliferation or morphological changes of cells.
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Mesenchymal stem cells are showing increasing promise in applications such as tissue engineering and cell therapy. MSC are low in number in bone marrow, and therefore in vitro expansion is often necessary. In vivo, stem cells often reside... more
Mesenchymal stem cells are showing increasing promise in applications such as tissue engineering and cell therapy. MSC are low in number in bone marrow, and therefore in vitro expansion is often necessary. In vivo, stem cells often reside within a niche acting to protect the cells. These niches are composed of niche cells, stem cells, and extracellular matrix. When blood vessels are damaged, a fibrin clot forms as part of the wound healing response. The clot constitutes a form of stem cell niche as it appears to maintain the stem cell phenotype while supporting MSC proliferation and differentiation during healing. This is particularly appropriate as fibrin is increasingly being suggested as a scaffold meaning that fibrin-based tissue engineering may to some extent recapitulate wound healing. Here, we describe how fibrin modulates the clonogenic capacity of MSC derived from young/old human donors and normal/diabetic rats. Fibrin was prepared using different concentrations to modulate...
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Research Interests: Technology, Flow Cytometry, Aging, Stem Cell, Apoptosis, and 14 moreBiological Sciences, Nitric oxide, Female, Animals, Temperature, Glutathione Peroxidase, Mesenchymal Stem Cell, Superoxide Dismutase, Rats, Homeostasis, Wistar Rats, Heat Shock Proteins, Oxidation-Reduction, and Mesenchymal Stromal Cells
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Research Interests: Aging, Stem Cell, Cardiovascular disease, Gene expression, Translational Medicine, and 18 moreAdipose tissue, Cell therapy, Cell Differentiation, Humans, Reactive Oxygen Species, Mice, Nitric oxide, Animals, TELOMERE LENGTH, Mesenchymal Stem Cell, Oxygen, HeLa cells, Growth Factor, Mesenchymal Stromal Cells, Plasminogen Activator Inhibitor, Vascular Endothelial Growth Factor, Matrix Metalloproteinase, and Transforming Growth Factor
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Research Interests: Neuroscience, Psychology, Protein Turnover, Proteasome, Nitric oxide, and 17 moreAnimals, Microglia, Lectins, Hallucinogens, Astrocytes, Rats, Homeostasis, Time Factors, Glial Fibrillary Acidic Protein, Age Factors, Multienzyme complexes, Wistar Rats, Cysteine endopeptidases, Amphetamines, Cell Survival, Neurosciences, and Adenosine Triphosphate
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Research Interests: Free Radicals, Free Radical, Age, Glucose, Proteasome, and 13 moreNitric oxide, Animals, Microglia, Fructose, Oxidative stress and free radical biology and medicine, Rats, Tumor necrosis factor-alpha, Wistar Rats, Bovine Serum Albumin, Lysosomes, Alzheimer Disease, Biochemistry and cell biology, and Serum albumin
Research Interests: Free Radicals, Adipose tissue, Small Mammals, Proteasome, Reactive Oxygen Species, and 19 moreBat, Animals, Body temperature regulation, Proteins, Food intake, Oxidative stress and free radical biology and medicine, Brown Adipose Tissue, Skeletal Muscle, Oxidative Damage, Multienzyme complexes, Cysteine endopeptidases, Arvicolinae, Resting Metabolic Rate, Oxygen Consumption, Oxidation-Reduction, Small Intestine, Biochemistry and cell biology, Chymotrypsin, and Protein Oxidation
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Research Interests: Free Radicals, Flow Cytometry, Bone Metabolism, Cell Biology, Apoptosis, and 25 moreMacromolecular X-Ray Crystallography, Free Radical, Mesenchymal stem cells, Blood Glucose, Diabetes mellitus, Trabecular Bone, Tibia, Animals, Male, Polymerase Chain Reaction, Mesenchymal Stem Cell, Cardio vascular disease, Medical Physiology, Alkaline phosphatase, Rats, Bone Loss, Computerized tomography, Body Weight, Bone Formation, Streptozotocin, Experimental Diabetes Mellitus Type 1 and 2, Diabetic Rat, Cell Survival, Mesenchymal Stromal Cells, and Biochemistry and cell biology
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Research Interests: Cognitive Science, Free Radicals, Oxidative Stress, Free Radical, Antioxidants, and 18 moreCell line, Protein Turnover, Proteasome, Animals, Turnover, Central Nervous System, Iron, Rats, Rat, Oxidative Damage, Multienzyme complexes, Cysteine endopeptidases, Nitrogen Oxides, Neurosciences, Oxidation-Reduction, Neuroprotective Agents, Intracellular, and Protein Oxidation
Mesenchymal stem cells (MSC) are multipotent cells capable of differentiating into a number of mesenchymal tissues including bone, cartilage, and tendon. Low numbers in vivo means exponential growth is needed in culture to enable... more
Mesenchymal stem cells (MSC) are multipotent cells capable of differentiating into a number of mesenchymal tissues including bone, cartilage, and tendon. Low numbers in vivo means exponential growth is needed in culture to enable therapeutic applications. MSC can expand rapidly in culture but usually lose their extensive capacity for differentiation that makes them therapeutically attractive. To try and maintain their capacity for differentiation and expansion in vitro, we cultured MSC on fibrin gels of different concentrations to create more physiological growth conditions for the cells. The cells were then re-plated onto tissue culture plastic and analysed. The cells that had been pre-cultured for seven days on fibrin, proliferated and maintained their differential potential to the osteogenic lineage better than tissue culture plastic expanded MSC. A concentration relationship between colony number and fibrin concentration was seen with decreasing numbers as fibrin concentration increased. These data support the concept that substrate signals significantly influence MSC growth and differentiation and that growth on a fibrin matrix could be used to maintain a stem cell phenotype during MSC expansion.
Research Interests: Biomedical Engineering, Stem Cell, Cell Differentiation, Fibrin, Humans, and 18 moreTissue culture, Biomedical Materials, Medical Biotechnology, Mice, Animals, Plastics, Mesenchymal Stem Cell, Phenotype, Cattle, Gels, Mechanical Stress, Exponential Growth, Osteogenesis, Cell Proliferation, Osteoblasts, Culture Media, Mesenchymal Stromal Cells, and Bone Marrow Cells
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Research Interests: Fluorescence Microscopy, Enzyme Inhibitors, Macrophages, Mice, Nitric oxide, and 15 moreAnimals, Microglia, Hydrogen Peroxide, Lipopolysaccharides, Rats, Tumor necrosis factor-alpha, Protein Degradation, Wistar Rats, Proteases, Oxidation-Reduction, Extracellular Space, Oxidants, Biochemistry and cell biology, Protein Oxidation, and Laminin
Research Interests: Free Radicals, Oxidative Stress, Mitochondria, Cell Culture, Free Radical, and 20 moreAntioxidants, Cell line, Brain, Protein Turnover, Animals, Cell Viability, Paraquat, Superoxide Dismutase, Hydrogen Peroxide, Metabolic rate, Rats, Alpha Lipoic Acid, Oxidative Damage, Demyelinating disease, Cell Survival, Enzyme Linked Immunosorbent Assay, Biochemistry and cell biology, Immunoblotting, Protein Oxidation, and Mitochondrial Proteins
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In this study, we investigated the presence and role of immunoproteasome and its LMP2 subunit polymorphism at codon 60 in Alzheimer's disease (AD). Immunoproteasome was present in brain areas such as hippocampus and cerebellum and... more
In this study, we investigated the presence and role of immunoproteasome and its LMP2 subunit polymorphism at codon 60 in Alzheimer's disease (AD). Immunoproteasome was present in brain areas such as hippocampus and cerebellum and localized in neurons, astrocytes and endothelial cells. A higher expression of immunoproteasome was found in brain of AD patients than in brain of non-demented elderly, being its expression in young brain negligible or absent. Furthermore, AD affected regions showed a partial decrease in ...