Maejo International Journal of Energy and Environmental Communication
Nowadays, the water pollution is leading issue due to palm oil mill effluent (POME), waste from p... more Nowadays, the water pollution is leading issue due to palm oil mill effluent (POME), waste from palm oil production is a big problem to Malaysia which is largest palm oil producers. POME containing large amount of chemical oxygen demand (COD) which can cause severe pollution to the environment especially water. This present study designed for culturing green algae (Chlorella sp.) isolated from Pantai Balok, Kuantan which is most common microalgae with palm oil mill effluent (POME). Chlorella sp. was cultured using BG-11 medium with the addition of POME as experiment and without POME as control. The POME responsible for the growth of green algae is studied by measurement of the growth rate, total cell count and chemical oxygen demand (COD) for both conditions. The composition for control and experiment is also measured and determined by using Gas Chromatography Mass Spectrometry (GCMS). From the results, it is observed that the total cell count and growth rate of Chlorella sp. greate...
The use of alternative biomass sources that are not competitive with food production is intended ... more The use of alternative biomass sources that are not competitive with food production is intended for sustainable management in biogas production through anaerobic digestion. This study investigates the Napier grass and cattle slurrybased biogas production application that could be applied more cost-effectively more sustainable production biogas. The laboratory-based biogas plant and a biogas plant in practice revealed that the results from the laboratory experiments were realistic and transferable into practice. The effect of feedstock screening on the biogas yield of Napier grass and cattle slurry was evaluated in batch digesters under mesophilic conditions. Moreover, highest methane content was reached 64.4%. The biogas from the co-digestion of Napier grass and cow farm slurry containing the higher calorific value was 25.69 MJ/m3, and the lower calorific value was 23.14 MJ/m3. The results demonstrated that combining Napier grass with common cow farm slurry can accelerate the react...
This study aims to produce a significant quantity of fermentable sugars from tubers sourced from ... more This study aims to produce a significant quantity of fermentable sugars from tubers sourced from northern Thailand to boost bioethanol production. Bioethanol production from Amorphophallus spp. tubers was compared using biological and chemical approaches. The tubers were sliced into small pieces (1–2 cm), dried in a solar oven, and powdered before hydrolyzing with cellulase enzymes. The results revealed the fermentable sugar content of Amorphophallus spp. tuber increased from 2.6 g/L–19.01 g/L after enzymatic hydrolysis. Furthermore, under the ideal condition, the total sugar concentration was 33.22 g/L. Enzymes help to speed up the hydrolysis process, and biological methods are also less expensive and more ecologically friendly than chemical equivalents. After the alcoholic fermentation, the highest ethanol content was obtained, 8.68 ± 0.91 g/L, using S. cerevisiae for 48 h. Konjac receives little attention in the biofuel industry due to its irritating nature to the mouth and throa...
The large number of wastewaters are generated because of the various production processes. Vegeta... more The large number of wastewaters are generated because of the various production processes. Vegetable and fish processing can be considered an important industry for wastewater generation. The essential method for completing this waste is to digest the organic matter using anaerobic digestion followed by aerobic wastewater treatment processes; however, wastewater from tilapia culture pond retains considerable quantities of inorganic substances, particularly nutrients like nitrogen and phosphorus. The optimal conditions for cultivating Chlorella vulgaris from wastewater treatment effluent from tilapia culture pond were investigated in this study. The appropriate conditions were found to be 10% initial stock suspension, 20 cm depth, and 12 days of culture conditions. C. vulgaris had an optical density of 0.649, a cell density of 17.68 × 105 cells/mL, and biomass of 0.376 ± 94.21 mg/L after cultivation. Discharged wastewater from the fishpond was utilized for the improved growth of micr...
Maejo International Journal of Energy and Environmental Communication
Nowadays, the water pollution is leading issue due to palm oil mill effluent (POME), waste from p... more Nowadays, the water pollution is leading issue due to palm oil mill effluent (POME), waste from palm oil production is a big problem to Malaysia which is largest palm oil producers. POME containing large amount of chemical oxygen demand (COD) which can cause severe pollution to the environment especially water. This present study designed for culturing green algae (Chlorella sp.) isolated from Pantai Balok, Kuantan which is most common microalgae with palm oil mill effluent (POME). Chlorella sp. was cultured using BG-11 medium with the addition of POME as experiment and without POME as control. The POME responsible for the growth of green algae is studied by measurement of the growth rate, total cell count and chemical oxygen demand (COD) for both conditions. The composition for control and experiment is also measured and determined by using Gas Chromatography Mass Spectrometry (GCMS). From the results, it is observed that the total cell count and growth rate of Chlorella sp. greate...
Maejo International Journal of Energy and Environmental Communication
This study aimed to investigate the effect of nutrient stress such as nitrogen and phosphorus on ... more This study aimed to investigate the effect of nutrient stress such as nitrogen and phosphorus on the growth rate of green algae, Chlorella sp. and the mechanism secreted to survive during the depletion of nutrients. The sample was tested with several vital parameters, suppressed Total Nitrogen (TN) and Total Phosphorus (TP). A process known as acclimatization was carried out to ensure that the algae can adapt to a new environment which was repeated 2 times, each taking about 2 weeks. The active compounds in the samples were measured using Gas Chromatography-Mass Spectrophotometry. The experiments showed that the control microalgae secreted hexadecanoic acid and methyl stearate while nitrogen suppressed microalgae secreted Undecanoic acid, 10-methyl- and methyl ester. Phosphorus limited microalgae secreted 1,3-Propanediamine, N-(2-aminoethyl)- due to suppress of nutrients. This experiment should be tested in several study areas in Mexico and outside Mexico to compare the productivity...
Maejo International Journal of Energy and Environmental Communication
Due to high demand in the industry field, fuel supply has been reduced due to overconsumption. Al... more Due to high demand in the industry field, fuel supply has been reduced due to overconsumption. Algae served as the most promising biofuels; Cyanobacteria is preferred as a material for producing biodiesel based on the comparative advantage. Cyanobacteria can produce high lipids profiles that can be used to produce biofuel or biodiesel. The present investigation aimed to identify the application of Cyanobacteria that have proposed lipid standards for the algae biofuel industry. Three main objectives are involved in these experiments: to isolate and identify different strains of Cyanobacteria, convert the lipid from microalgae into biodiesel through transesterification, and estimate the proposed lipid standard of Cyanobacteria for the algae biofuel industry. The microalgae involve isolated using a plankton net. The water sample is brought into the lab for the identification of Cyanobacteria. After the Cyanobacteria are identified, the Cyanobacteria undergo mass cultivation to ensure t...
The large number of wastewaters are generated because of the various production processes. Vegeta... more The large number of wastewaters are generated because of the various production processes. Vegetable and fish processing can be considered an important industry for wastewater generation. The essential method for completing this waste is to digest the organic matter using anaerobic digestion followed by aerobic wastewater treatment processes; however, wastewater from tilapia culture pond retains considerable quantities of inorganic substances, particularly nutrients like nitrogen and phosphorus. The optimal conditions for cultivating Chlorella vulgaris from wastewater treatment effluent from tilapia culture pond were investigated in this study. The appropriate conditions were found to be 10% initial stock suspension, 20 cm depth, and 12 days of culture conditions. C. vulgaris had an optical density of 0.649, a cell density of 17.68 × 105 cells/mL, and biomass of 0.376 ± 94.21 mg/L after cultivation. Discharged wastewater from the fishpond was utilized for the improved growth of micr...
Maejo International Journal of Energy and Environmental Communication
Nowadays, the water pollution is leading issue due to palm oil mill effluent (POME), waste from p... more Nowadays, the water pollution is leading issue due to palm oil mill effluent (POME), waste from palm oil production is a big problem to Malaysia which is largest palm oil producers. POME containing large amount of chemical oxygen demand (COD) which can cause severe pollution to the environment especially water. This present study designed for culturing green algae (Chlorella sp.) isolated from Pantai Balok, Kuantan which is most common microalgae with palm oil mill effluent (POME). Chlorella sp. was cultured using BG-11 medium with the addition of POME as experiment and without POME as control. The POME responsible for the growth of green algae is studied by measurement of the growth rate, total cell count and chemical oxygen demand (COD) for both conditions. The composition for control and experiment is also measured and determined by using Gas Chromatography Mass Spectrometry (GCMS). From the results, it is observed that the total cell count and growth rate of Chlorella sp. greate...
The use of alternative biomass sources that are not competitive with food production is intended ... more The use of alternative biomass sources that are not competitive with food production is intended for sustainable management in biogas production through anaerobic digestion. This study investigates the Napier grass and cattle slurrybased biogas production application that could be applied more cost-effectively more sustainable production biogas. The laboratory-based biogas plant and a biogas plant in practice revealed that the results from the laboratory experiments were realistic and transferable into practice. The effect of feedstock screening on the biogas yield of Napier grass and cattle slurry was evaluated in batch digesters under mesophilic conditions. Moreover, highest methane content was reached 64.4%. The biogas from the co-digestion of Napier grass and cow farm slurry containing the higher calorific value was 25.69 MJ/m3, and the lower calorific value was 23.14 MJ/m3. The results demonstrated that combining Napier grass with common cow farm slurry can accelerate the react...
This study aims to produce a significant quantity of fermentable sugars from tubers sourced from ... more This study aims to produce a significant quantity of fermentable sugars from tubers sourced from northern Thailand to boost bioethanol production. Bioethanol production from Amorphophallus spp. tubers was compared using biological and chemical approaches. The tubers were sliced into small pieces (1–2 cm), dried in a solar oven, and powdered before hydrolyzing with cellulase enzymes. The results revealed the fermentable sugar content of Amorphophallus spp. tuber increased from 2.6 g/L–19.01 g/L after enzymatic hydrolysis. Furthermore, under the ideal condition, the total sugar concentration was 33.22 g/L. Enzymes help to speed up the hydrolysis process, and biological methods are also less expensive and more ecologically friendly than chemical equivalents. After the alcoholic fermentation, the highest ethanol content was obtained, 8.68 ± 0.91 g/L, using S. cerevisiae for 48 h. Konjac receives little attention in the biofuel industry due to its irritating nature to the mouth and throa...
The large number of wastewaters are generated because of the various production processes. Vegeta... more The large number of wastewaters are generated because of the various production processes. Vegetable and fish processing can be considered an important industry for wastewater generation. The essential method for completing this waste is to digest the organic matter using anaerobic digestion followed by aerobic wastewater treatment processes; however, wastewater from tilapia culture pond retains considerable quantities of inorganic substances, particularly nutrients like nitrogen and phosphorus. The optimal conditions for cultivating Chlorella vulgaris from wastewater treatment effluent from tilapia culture pond were investigated in this study. The appropriate conditions were found to be 10% initial stock suspension, 20 cm depth, and 12 days of culture conditions. C. vulgaris had an optical density of 0.649, a cell density of 17.68 × 105 cells/mL, and biomass of 0.376 ± 94.21 mg/L after cultivation. Discharged wastewater from the fishpond was utilized for the improved growth of micr...
Maejo International Journal of Energy and Environmental Communication
Nowadays, the water pollution is leading issue due to palm oil mill effluent (POME), waste from p... more Nowadays, the water pollution is leading issue due to palm oil mill effluent (POME), waste from palm oil production is a big problem to Malaysia which is largest palm oil producers. POME containing large amount of chemical oxygen demand (COD) which can cause severe pollution to the environment especially water. This present study designed for culturing green algae (Chlorella sp.) isolated from Pantai Balok, Kuantan which is most common microalgae with palm oil mill effluent (POME). Chlorella sp. was cultured using BG-11 medium with the addition of POME as experiment and without POME as control. The POME responsible for the growth of green algae is studied by measurement of the growth rate, total cell count and chemical oxygen demand (COD) for both conditions. The composition for control and experiment is also measured and determined by using Gas Chromatography Mass Spectrometry (GCMS). From the results, it is observed that the total cell count and growth rate of Chlorella sp. greate...
Maejo International Journal of Energy and Environmental Communication
This study aimed to investigate the effect of nutrient stress such as nitrogen and phosphorus on ... more This study aimed to investigate the effect of nutrient stress such as nitrogen and phosphorus on the growth rate of green algae, Chlorella sp. and the mechanism secreted to survive during the depletion of nutrients. The sample was tested with several vital parameters, suppressed Total Nitrogen (TN) and Total Phosphorus (TP). A process known as acclimatization was carried out to ensure that the algae can adapt to a new environment which was repeated 2 times, each taking about 2 weeks. The active compounds in the samples were measured using Gas Chromatography-Mass Spectrophotometry. The experiments showed that the control microalgae secreted hexadecanoic acid and methyl stearate while nitrogen suppressed microalgae secreted Undecanoic acid, 10-methyl- and methyl ester. Phosphorus limited microalgae secreted 1,3-Propanediamine, N-(2-aminoethyl)- due to suppress of nutrients. This experiment should be tested in several study areas in Mexico and outside Mexico to compare the productivity...
Maejo International Journal of Energy and Environmental Communication
Due to high demand in the industry field, fuel supply has been reduced due to overconsumption. Al... more Due to high demand in the industry field, fuel supply has been reduced due to overconsumption. Algae served as the most promising biofuels; Cyanobacteria is preferred as a material for producing biodiesel based on the comparative advantage. Cyanobacteria can produce high lipids profiles that can be used to produce biofuel or biodiesel. The present investigation aimed to identify the application of Cyanobacteria that have proposed lipid standards for the algae biofuel industry. Three main objectives are involved in these experiments: to isolate and identify different strains of Cyanobacteria, convert the lipid from microalgae into biodiesel through transesterification, and estimate the proposed lipid standard of Cyanobacteria for the algae biofuel industry. The microalgae involve isolated using a plankton net. The water sample is brought into the lab for the identification of Cyanobacteria. After the Cyanobacteria are identified, the Cyanobacteria undergo mass cultivation to ensure t...
The large number of wastewaters are generated because of the various production processes. Vegeta... more The large number of wastewaters are generated because of the various production processes. Vegetable and fish processing can be considered an important industry for wastewater generation. The essential method for completing this waste is to digest the organic matter using anaerobic digestion followed by aerobic wastewater treatment processes; however, wastewater from tilapia culture pond retains considerable quantities of inorganic substances, particularly nutrients like nitrogen and phosphorus. The optimal conditions for cultivating Chlorella vulgaris from wastewater treatment effluent from tilapia culture pond were investigated in this study. The appropriate conditions were found to be 10% initial stock suspension, 20 cm depth, and 12 days of culture conditions. C. vulgaris had an optical density of 0.649, a cell density of 17.68 × 105 cells/mL, and biomass of 0.376 ± 94.21 mg/L after cultivation. Discharged wastewater from the fishpond was utilized for the improved growth of micr...
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