Dr. Prakash Bhuyar
Maejo University, International College (MJU-IC), Faculty Member
- Maejo University, School of Renewable Energy, Faculty Memberadd
- Dear Colleagues: I am looking forward to collaborating with you in writing joint funding proposals, scientific manusc... moreDear Colleagues: I am looking forward to collaborating with you in writing joint funding proposals, scientific manuscripts, reviews, book chapters, organizing conferences, or workshops. Please contact me at my email address for further discussion.My email is: prasadmbhuyar@gmail.comedit
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Research Interests: Horticulture and Greening
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Anaerobic digestion is recognized as an attractive option for the effective management and treatment of lignocellulosic biomass as well as waste recovery of resources for bioethanol production. Long enough testing has been done on... more
Anaerobic digestion is recognized as an attractive option for the effective management and treatment of lignocellulosic biomass as well as waste recovery of resources for bioethanol production. Long enough testing has been done on bioethanol production using lignocellulosic biomass. This helps to reduce stress and global energy problems. Global wide has a variety of environmental impacts due to its use of fossil fuels. Bioethanol might be produced in Asian locations from many types of biomasses, including agricultural waste, forest waste, and wood biomass. This would be an environmentally friendly process. Unfortunately, there is very little research into the production of ethanol from rice field weeds. This makes it difficult to develop bioethanol production. This review is aimed at developing bioethanol production and the trend towards organic products that began nowadays. Unwanted weed growth is a major problem in rice cultivation. This review demonstrated the waste-to-energy asp...
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Biofuels have been regaining popularity due to the increasing price of non-renewable fuels and the higher carbon dioxide emissions. Biofuels are manufactured from plant products and are mainly composed of lignocellulose and starch... more
Biofuels have been regaining popularity due to the increasing price of non-renewable fuels and the higher carbon dioxide emissions. Biofuels are manufactured from plant products and are mainly composed of lignocellulose and starch materials. This investigation aims to produce increased fermentable sugars for enhanced bioethanol production from tubers procured from northern Thailand. Varying concentrations of H2SO4 is used to pretreat the tubers. Before hydrolyzing with cellulase enzymes, the tubers were chopped into small pieces (1-2 cm), dried in a solar oven, powdered. The obtained results confirmed that the fermentable/ reducing sugar content of Amorphophallus spp. (suweg) tuber increased from 2.6 g/L to 19.01 g/L after enzymatic hydrolysis. The enzymes act as an excellent way to speed up the hydrolysis process. The theoretical potential of bioethanol production was calculated under ideal conditions, with the highest bioethanol concentration obtained is 9.69 ± 0.12 g/L at 0.4 % H...
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Gentiana kurroo royle is an endangered bitter medicinal plant of the Indian subcontinent. This medicinal plant mainly grows in Kashmir, Himachal Pradesh, and North-west Himalayas. The medicinal plant's root and rhizome are frequently... more
Gentiana kurroo royle is an endangered bitter medicinal plant of the Indian subcontinent. This medicinal plant mainly grows in Kashmir, Himachal Pradesh, and North-west Himalayas. The medicinal plant's root and rhizome are frequently used by native people for various local remedies. This species has become critically endangered due to its endemic nature and high rate of extraction from its natural environment. In India rhizome and root of this medicinal plant are used as ant periodic, bitter tonics, anti-inflammatory, blood purifier, expectorant, antipsychotic, carminative, and stomachic. Genitiana kurroo can be propagated through micro-filtration of seeds, rhizome cutting, shoot-made segments, and somatic embryogenesis. The roots of Genitiana kurroo are rich in various ingredients such as flavonoids, alkaloids, and terpenoids which are responsible for analgesic, anticancer, and immune-modular effects. Considering these perspectives the present review has intended to provide upd...
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The aim of this study is to determine the quality of artificially inoculated agarwood from Aquilaria malaccensis trees obtained from a plantation in East Malaysia using gas chromatography (GC). The agarwood quality was measured by... more
The aim of this study is to determine the quality of artificially inoculated agarwood from Aquilaria malaccensis trees obtained from a plantation in East Malaysia using gas chromatography (GC). The agarwood quality was measured by existence and amount of aromatic and terpene compounds especially those which were recorded in high-quality agarwood such as α-guaiene, β-selinene, aromadendrene and agarospirol. For quality determination purpose of artificially inoculated agarwood, samples were collected from selected plantation area. Solid Phase Micro Extraction (SPME) method was performed to collect the volatile compounds released by wood sample. There were five commercial inoculants (Ino A, B, C, D and E) that had been used for inoculation of A. malaccensis in agarwood plantation. GC analysis revealed the presence of important compounds related to high-quality agarwood such as 4-phenyl-2-butanone, β-selinene, α-bulnesene, and agarospirol in agarwood sample produced from artificial inoc...
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Agarwood (Aquilaria malaccensis) oil is one of the essential bioactive materials in the scent/ perfume industry for the middle east and gulf countries. The built-in functionality and availability of bioactive components of oil make... more
Agarwood (Aquilaria malaccensis) oil is one of the essential bioactive materials in the scent/ perfume industry for the middle east and gulf countries. The built-in functionality and availability of bioactive components of oil make agarwood more precious. In this study, the physically induced infected agarwood tree chops were extracted by microwave assisted Soxhlet extraction. Moreover, the extracted oil samples were analyzed against control not infected trees using Gas chromatography-mass spectroscopy (GC-MS) by GCMS (Agilent GC 6890zN MS 5973). The microwave assisted Soxhlet extraction revealed a higher amount of oil extraction. In the physically induced samples, 24 signal peaks were recorded employing GC-MS. The most prominent peak area recorded was -5-hydroxy-3-methyl-1-indanone detected at minute 41.89 with a peak area of 17.64%, followed by -methyl 4,4,7-trimethyl 4.7-dihydro indan -6- carboxylate at 23.17 min, occupying an area of 15.54%; followed by 3-carene recorded at 46.6...
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The Niamhom (Strobilanthes nivea Craib) plant was cultivated using a modern greenhouse with an automatic irrigation system controlled by the Internet of Things (IoT). The present study investigates the effects of fertilizers, including... more
The Niamhom (Strobilanthes nivea Craib) plant was cultivated using a modern greenhouse with an automatic irrigation system controlled by the Internet of Things (IoT). The present study investigates the effects of fertilizers, including chemicals, cow manure fertilizer, and bio-fermented water with shading influences of 50 and 70% on plant growth and morphological characteristics. Additionally, the study aims to analyze the chemical constituents of cultivated Niamhom leaf evaluated by Gas Chromatography-Mass Spectrometry (GC-MS). Findings discovered that 50 and 70% shading did not affect plant stem size and canopy diameter. However, shading at 50% affects chlorophyll content, yield, dry weight, leaf diameter, plant height, and the number of leaves. There were no differences in plant growth parameters among the three fertilizer treatments, but significant differences were found in leaf number, leaf area, and chlorophyll content. The chemical fertilizers gave the highest values, follow...
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The large number of wastewaters are generated because of the various production processes. Vegetable and fish processing can be considered an important industry for wastewater generation. The essential method for completing this waste is... more
The large number of wastewaters are generated because of the various production processes. Vegetable and fish processing can be considered an important industry for wastewater generation. The essential method for completing this waste is to digest the organic matter using anaerobic digestion followed by aerobic wastewater treatment processes; however, wastewater from tilapia culture pond retains considerable quantities of inorganic substances, particularly nutrients like nitrogen and phosphorus. The optimal conditions for cultivating Chlorella vulgaris from wastewater treatment effluent from tilapia culture pond were investigated in this study. The appropriate conditions were found to be 10% initial stock suspension, 20 cm depth, and 12 days of culture conditions. C. vulgaris had an optical density of 0.649, a cell density of 17.68 × 105 cells/mL, and biomass of 0.376 ± 94.21 mg/L after cultivation. Discharged wastewater from the fishpond was utilized for the improved growth of micr...
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In the present study, green synthesis of silver-nanoparticle (AgNPs) is demonstrated using plant extract of Vitex negundo. Plant extract through six different solvents, including petroleum ether, benzene, chloroform, acetone, methanol,... more
In the present study, green synthesis of silver-nanoparticle (AgNPs) is demonstrated using plant extract of Vitex negundo. Plant extract through six different solvents, including petroleum ether, benzene, chloroform, acetone, methanol, and water, was prepared and further investigated for its antimicrobial and antifungal activities using different bacterial and fungal strains. The phytochemical analysis was performed, where saponins, tannins, steroids, flavonoids and glycosides were detected in acetone, chloroform and methanolic extract. Subsequent analysis of synthesized AgNPs through dynamic light scattering suggested that particle sizes were 10-300 nm in size. The study indicated that the chloroform, acetone, and methanol extracts of Vitex negundo showed good inhibitory activity against Staphylococcus aureus, Pseudomonas aeruginosa, Bacillus subtilis based on the zone of inhibition. The antimicrobial activity of the synthesized AgNPs suggested that it can inhibit the growth of bot...
Anaerobic digestion (AD) is a commercial technology for bioenergy technology, which is a naturally occurring process of various microbial actions that break down organic waste materials and produce energy as biogas. Nevertheless, AD... more
Anaerobic digestion (AD) is a commercial technology for bioenergy technology, which is a naturally occurring process of various microbial actions that break down organic waste materials and produce energy as biogas. Nevertheless, AD efficiency can be reduced due to substrate loading, ammonia inhibition, low methane yield, and impure feedstock. The supplementation of various metal nanoparticles (NPs) has shown significant results for higher biogas production in the AD process. Iron (Fe)based NPs are an essential complement to microbes for higher biogas production in the AD process. In this study, Fe-Co-Cu TMNPs were synthesized via the chemical co-precipitation method using metal salt (Fe(NO 3) 3 , Co(NO 3) 2 , Cu(NO 3) 2), and precipitants. The structure and morphology of the TMNPs are characterized by Fourier-transformation (FTIR) spectroscopy, X-ray diffraction (XRD), and field-emission scanning electron microscopy (FESEM/EDX). Furthermore, the effect of Fe-Co-Cu trimetallic nanoparticles (TMNPs) in five different concentrations (0, 10, 20, 30, 40, and 50 mg/L) on palm oil mill effluent (POME)-based AD process for biogas production. The results showed that biogas yield increased by 5.66%, 7.54%, and 11.11% when using 20, 30, and 50 mg/L Fe-Co-Cu TMNPs. With the same TMNP concentration (10 mg/L and 40 mg/L), biogas yield was reduced by 26.41% and 11.11%, respectively. The economic feasibility and sustainability of Fe-Co-Cu TMNPs manufacturing and applying Fe-Co-Cu TMNPs in the AD process have been demonstrated. The assay showed the negative and positive effects of Fe-Co-Cu TMNPs in the POME-based AD process. The result of the present study indicates a possible new strategy for the preparation and design of NPs to enhance biogas production.
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The mushroom cultivation industry in Malaysia is one of the fastest growing agricultural sectors due to its high return value and low production cost. With the Malaysian government forecasting an RM300 million worth of mushroom exporting... more
The mushroom cultivation industry in Malaysia is one of the fastest growing agricultural sectors due to its high return value and low production cost. With the Malaysian government forecasting an RM300 million worth of mushroom exporting by 2020, demand for high-quality mushrooms would rise. Commercially grown mushrooms in Malaysia include Pleurotus ostreatus, Pleurotus djamor, Pleurotus eryngii, and Schizophyllum commune. The mushroom cultivating technique is critical to achieving large yields. More mushrooms can be harvested in less time by employing Effective Microbes (EM) as a yield performance booster. However, EM is not yet commercially accessible, despite the fact that numerous potential EM exist, including dominant bacteria from mushroom production, bacteria bioinoculant, and anti-listerial agent. Furthermore, the EM activator found inside the substrate represents another yet-to-be-commercialized yield performance booster. Several possible EM activators, including as dairy w...
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Present work proposes on the synthesis of nano calcium oxide derived from waste cockle shell (Anadara granosa) and applied as heterogenous catalyst in biodiesel production via transesterification reaction under reaction parameter 15:1... more
Present work proposes on the synthesis of nano calcium oxide derived from waste cockle shell (Anadara granosa) and applied as heterogenous catalyst in biodiesel production via transesterification reaction under reaction parameter 15:1 methanol to oil ratio, 5 wt.% catalyst and 3 hour reation time and constant temperature at 65 ± 2 °C. The waste cockle shell was prepared in nano size via ball mill technique which was run for 36 hours at 350 rpm speed and then calcined at 900 °C for 2 hours to decompose calcium carbonate, CaCO₃ into nano calcium oxide, n-CaO. The catalyst was characterized by particle size analyzer, Transmission Electron Microscopy, Field Emission Scanning Electron Microscope, Surface Area Analyser, X-Ray Diffractometer, Fourier transform infrared, Hammet indicator and benzoic acid titration. Particle size of n-CaO catalyst was found within the range of 29 nm to 67.5 nm. Specific surface area of synthesised n-CaO is 8.41 m²/g with basicity strength of 0.055 mmol/g. ...
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Nowadays, the water pollution is leading issue due to palm oil mill effluent (POME), waste from palm oil production is a big problem to Malaysia which is largest palm oil producers. POME containing large amount of chemical oxygen demand... more
Nowadays, the water pollution is leading issue due to palm oil mill effluent (POME), waste from palm oil production is a big problem to Malaysia which is largest palm oil producers. POME containing large amount of chemical oxygen demand (COD) which can cause severe pollution to the environment especially water. This present study designed for culturing green algae (Chlorella sp.) isolated from Pantai Balok, Kuantan which is most common microalgae with palm oil mill effluent (POME). Chlorella sp. was cultured using BG-11 medium with the addition of POME as experiment and without POME as control. The POME responsible for the growth of green algae is studied by measurement of the growth rate, total cell count and chemical oxygen demand (COD) for both conditions. The composition for control and experiment is also measured and determined by using Gas Chromatography Mass Spectrometry (GCMS). From the results, it is observed that the total cell count and growth rate of Chlorella sp. greate...
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Abstract Optimization of biodiesel production from freshwater macroalgae, Rhizoclonium sp. was investigated in this study. Biodiesel production process parameters such as chemical solvent extraction, hexane:ether and different extraction... more
Abstract Optimization of biodiesel production from freshwater macroalgae, Rhizoclonium sp. was investigated in this study. Biodiesel production process parameters such as chemical solvent extraction, hexane:ether and different extraction techniques were optimized by using the response surface methodology based on the central composite design. Optimization of the transesterification process was conducted by varying two factors each at three different levels and this required a total of thirteen runs. A quadratic model was created to predict the biodiesel yield where the R2 value was found to be 0.97, which indicates the satisfactory accuracy of the model. Based on the results, the optimum process parameters for transesterification of the macroalgae Rhizoclonium sp. oil mixture at an agitation speed of 300 rpm over a period of 180 min was found to be a hexane:ether molar ratio 1:1(40 mL), NaOH catalyst concentration of 1 wt% and reaction temperature of 45 °C. Finally, a process optimization found highest macroalgae oil by simple treatment was 0.376 ± 0.14 g and ultrasonic treatment was 6.044 ± 0.81 g is the highest of biodiesel weight. For transesterification reaction from macroalgae oil, a triglyceride was mixed with 0.25 g methanol converted to biodiesel into methyl esters. The biodiesel weight 0.174 ± 0.034 g and 82.2% of the total fatty acid methyl esters (FAME) were confirmed from gas chromatography (GC) analysis. The biodiesel properties were characterized and the results obtained. Design-Expert Version 11 was used to draw both 3D surface plots and 2D contour plots to predict the optimum biodiesel yield.
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Background Marine algae used as a food source for ocean life and range in color from red to green to brown grow along rocky shorelines around the world. The synthesis of silver nanoparticles by marine alga Padina sp. and its... more
Background Marine algae used as a food source for ocean life and range in color from red to green to brown grow along rocky shorelines around the world. The synthesis of silver nanoparticles by marine alga Padina sp. and its characterization were fulfilled by using UV-visible spectrophotometer, Fourier transform infrared spectroscopy, scanning electron microscopy and field emission scanning electron microscopy, and energy-dispersive X-ray spectroscopy. Results UV-visible absorption spectrum revealed that the formation of Ag nanoparticles was increased by the addition of marine algae and the spectral peak observed between a wavelength of ~ 420 nm and 445 nm. In addition, SEM and FESEM images examined the surface morphology and the size of the synthesized NPs was relatively uniform in size ~ 25–60 nm. Energy-dispersive X-ray spectroscopy analysis confirmed the purity of Ag NPs with atomic percentage of 48.34% Ag. The synthesized Ag NPs showed highly potent antibacterial activity. The ...
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The large number of wastewaters are generated because of the various production processes. Vegetable and fish processing can be considered an important industry for wastewater generation. The essential method for completing this waste is... more
The large number of wastewaters are generated because of the various production processes. Vegetable and fish processing can be considered an important industry for wastewater generation. The essential method for completing this waste is to digest the organic matter using anaerobic digestion followed by aerobic wastewater treatment processes; however, wastewater from tilapia culture pond retains considerable quantities of inorganic substances, particularly nutrients like nitrogen and phosphorus. The optimal conditions for cultivating Chlorella vulgaris from wastewater treatment effluent from tilapia culture pond were investigated in this study. The appropriate conditions were found to be 10% initial stock suspension, 20 cm depth, and 12 days of culture conditions. C. vulgaris had an optical density of 0.649, a cell density of 17.68 × 105 cells/mL, and biomass of 0.376 ± 94.21 mg/L after cultivation. Discharged wastewater from the fishpond was utilized for the improved growth of micr...
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In this present investigation, a packed-filter bioreactor was employed to produce hydrogen utilizing an expired soft drink as a substrate. The effects of feeding substrate concentrations ranging from 19.51, 10.19, 5.34, 3.48, to 2.51 g... more
In this present investigation, a packed-filter bioreactor was employed to produce hydrogen utilizing an expired soft drink as a substrate. The effects of feeding substrate concentrations ranging from 19.51, 10.19, 5.34, 3.48, to 2.51 g total sugar/L were examined, and the position of the packed filter installed in the bioreactor at dimensionless heights (h/H) of 1/4, 2/4, 3/4, and 4/4 was studied. The results revealed that with a substrate concentration of 20 g total sugar/L and a hydraulic retention time (HRT) of 1 h, a packed filter placed at the half-height position of the bioreactor (h/H 2/4) has the optimal hydrogen production rate, hydrogen yield, and average biomass concentration in the bioreactor, resulting in 55.70 ± 2.42 L/L/d, 0.90 ± 0.06 mol H2/mol hexose, and 17.86 ± 1.09 g VSS/L. When feeding substrate concentrations varied from 20, 10, to 5 g total sugar/L with the packed-filter position at h/H 2/4, Clostridium sp., Clostridium tyrobutyricum, and Bifidobacterium crudi...
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Background: Enzymes are proteins that catalyze the chemical reactions, such as biosynthetic and derivative, that occurring in living cells. In enzymatic reactions, the molecules at the beginning of process are called substrates and they... more
Background: Enzymes are proteins that catalyze the chemical reactions, such as biosynthetic and derivative, that occurring in living cells. In enzymatic reactions, the molecules at the beginning of process are called substrates and they are converted in to different molecules, called the product. Almost all processes in a biological cell need enzyme for catalytic reactions. The use of purified enzymes for generating a useful product or service constitutes enzyme technology. Enzymes have a wide variety of applications in industry, medicine research etc. Some out of this one industrially important enzyme is keratinase. It is an extracellular and proteolytic enzyme.
Medicinal plants are sources of important therapeutic aids for alleviating human ailments. The present research investigation was carried out to study anti-fungal activity of C. asiatica were tested against C. albicans, Aspergillus niger,... more
Medicinal plants are sources of important therapeutic aids for alleviating human ailments. The present research investigation was carried out to study anti-fungal activity of C. asiatica were tested against C. albicans, Aspergillus niger, and Penicillium sp. using two methods, disc diffusion method and broth dilution method. C. asiatica crude methanol extract was found to be the most effective against fungal activity. Compared to disc diffusion, broth dilution was a more appropriate method to quantitatively determine the anti-Candida activity of plant extract, whereby the MIC values of the crude extracts was determined. The result from disc diffusion assay demonstrates that plant extracts have an inhibitory effect. However, the broth dilution method result reveal that C. asiatica crude methanol extract has lower MIC values, meaning it has more prove that C. asiatica has an anti-fungal effect. Bioactive analysis results reveled that bioactive compounds present in the leaf, stems, roo...
Biotechnology Laboratory, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia. Algae Biotechnology Department, Institute of Biotechnology, Vietnam Academy... more
Biotechnology Laboratory, Faculty of Industrial Sciences & Technology, Universiti Malaysia Pahang, Lebuhraya Tun Razak, 26300 Gambang, Kuantan, Pahang, Malaysia. Algae Biotechnology Department, Institute of Biotechnology, Vietnam Academy of Science and Technology, 18 Hoang Quoc Viet Str., Cau Giay, Hanoi, Vietnam Microalgae Systematics and Applied Phycology Research Laboratory Science and Technology Research Center, Biology Department, De La Salle University 2401 Taft Avenue, Manila, Philippines