- Egypt
Amani Marzouk
Mansoura University, Pharmacology, Faculty Member
<p>Phytochemical investigation of <i>Gomphocarpus fruticosus</i> (L.) Ait. of Egyptian origin afforded the new pregnane glycoside... more
<p>Phytochemical investigation of <i>Gomphocarpus fruticosus</i> (L.) Ait. of Egyptian origin afforded the new pregnane glycoside lineolon-3-O-[<i>β</i>-D-oleandropyranosyl-(1–4)-<i>β</i>-D-cymaropyranosyl-(1–4)-<i>β</i>-D-cymaropyranoside], along with six known compounds. The structures of the isolated compounds were elucidated on the basis of extensive spectroscopic evidences derived from 1D, 2D NMR experiments, mass spectrometry and by comparing their physical and spectroscopic data to literature. These included the triterpenoids 3<i>β</i>-taraxerol, 3<i>β</i>-taraxerol acetate and betulinic acid, which are identified for the first time in <i>G</i>. <i>fruticosus</i> and the cardenolides uzarigenin, gomphoside and calotropin.</p
Research Interests:
Research Interests:
Research Interests:
Research Interests:
Microbial metabolism of danazol (17α-pregna-2,4-dien-20-yno[2,3-d]isoxazol-17ß-ol) by Beauveria bassiana ATCC 7159 and Glyocladium viride ATCC 10097 afforded four metabolites. The isolated metabolites were identified by different... more
Microbial metabolism of danazol (17α-pregna-2,4-dien-20-yno[2,3-d]isoxazol-17ß-ol) by Beauveria bassiana ATCC 7159 and Glyocladium viride ATCC 10097 afforded four metabolites. The isolated metabolites were identified by different spectroscopic techniques as 6ß- hydroxy danazol, which is a not yet reported danazol metabolite, 17ß-hydroxy-17α-pregn-4-en- 20-yn-3-one (ethisterone) and 17ß-hydroxy-2α-(hydroxymethyl)-17α-pregn-4-en-20-yn-3-one (2α- hydroxymethyl ethisterone), which represent the major danazol metabolites detected in human urine. The last metabolite, 6ß,17ß-dihydroxy-2-(hydroxymethyl)-17α-pregna-1,4-dien-20-yn-3-one, is also a minor human metabolite, for which the NMR data are described here for the first time. The metabolites were isolated in quantities that allowed their use for direct comparison in routine doping analysis
Research Interests: Chemistry, Metabolism, Biology, Magnetic Resonance Spectroscopy, Medicine, and 10 moreUrine, Beauveria, Fermentation, Liquid Chromatography / Electrospray Ionization Mass Spectrometry, Quntitative Thin Layer Chromatography, Metabolite, Doping in Sports, Danazol, Estrogen antagonists, and Pharmacology and pharmaceutical sciences
Research Interests:
Research Interests:
Six compounds were isolated from methanolic extracts of the leaf and the bark of Tipuana tipu growing in Egypt. The isolated compounds were identified as 1-Nonadecanol (1), β-sitosterol (2), Alpinumisoflavone (3), β-sitosterol glucoside... more
Six compounds were isolated from methanolic extracts of the leaf and the bark of Tipuana tipu growing in Egypt. The isolated compounds were identified as 1-Nonadecanol (1), β-sitosterol (2), Alpinumisoflavone (3), β-sitosterol glucoside (4), Protocatechualdehyde (5) and Protocatechuic acid (6), based on different spectroscopic data (UV, IR, NMR and MS). This is the first report for the isolation of compounds 1 & 3-6 from Tipuana tipu. Compound 1 is isolated for the first time from family Fabaceae. Compounds 2 and 3 showed a promising anticancer activity, while compound 5 displayed a remarkable in vivo anti-inflammatory activity compared to indomethacin. [Amen YM, Marzouk AM, Zaghloul MG, Afifi MS. Bioactive compounds from Tipuana tipu growing in Egypt. J Am Sci 2013;9(10):334-339]. (ISSN: 1545-1003). http://www.jofamericanscience.org. 44
Phytochemical examination of corncob extracts led to the isolation of a new lignan identified as 7,7'-dihydroxy-3'-O-demethyl-4-methoxymatairesinol, together with seven known compounds, identified as β-sitosterol,... more
Phytochemical examination of corncob extracts led to the isolation of a new lignan identified as 7,7'-dihydroxy-3'-O-demethyl-4-methoxymatairesinol, together with seven known compounds, identified as β-sitosterol, β-sitosteryl-β-D-glucoside, 6β-hydroxy-campest-4-en-3-one, 5α,8α-epidioxyergosta-6,22-dien-3β-ol, tricin, kaempferol and p-coumaric acid. The isolated compounds were identified by one and two-dimensional NMR spectroscopies and mass spectrometry.
Corncobs could serve as a substrate for citric acid production using solid state fermentation technique. The culture optimization concerning substrate concentration, culture duration, pH, temperature and substrate hydrolysis was carried... more
Corncobs could serve as a substrate for citric acid production using solid state fermentation technique. The culture optimization concerning substrate concentration, culture duration, pH, temperature and substrate hydrolysis was carried out for maximum productivity of citric acid. Under the optimized conditions, 48.4 g of citric acid was produced from 1 kg dry corncobs. Biological evaluation was carried out for citric acid such as melanin synthesis inhibitory, anti-allergy, anti-bacterial , and hyaluronic acid production activities. The results showed that citric acid has potent me-lanin inhibitory activity, good inhibition for β-hexosaminidase release and potent stimulatory effect for the production of hyaluronic acid. These activities (melanin synthesis inhibitory, anti-allergy and hyaluronic acid productive activities) of citric acid have been reported for the first time.
Phytochemical investigation of Volutaria abyssinica (A. Rich.) C. Jeffrey led to the isolation of three sesquiterpene lactones, viz., amberboin, lipidiol and cynaropicrin along with daucosterol and 20-hydroxy ecdysone. All these compounds... more
Phytochemical investigation of Volutaria abyssinica (A. Rich.) C. Jeffrey led to the isolation of three sesquiterpene lactones, viz., amberboin, lipidiol and cynaropicrin along with daucosterol and 20-hydroxy ecdysone. All these compounds are identified for the first time in the plant. Their structures were determined based on chemical and spectroscopic data. The sesquiterpene lactones were evaluated for their in vitro cytotoxicity against four human tumor cell lines and the results were explained through a molecular modeling study. [Marzouk, A M. Investigating Volutaria abyssinica as a potential source for cytotoxic sesquiterpenoids. J Am Sci 2015;11(8):73-81]. (ISSN: 1545-1003). http://www.jofamericanscience.org
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Research Interests:
The essential oil isolated by hydrodistillation from the fresh leaves of the locally cultivated Citrus aurantium L. "Russian colon" has been analyzed by GC-MS. Twenty three components accounting to 94.38% of the total detected... more
The essential oil isolated by hydrodistillation from the fresh leaves of the locally cultivated Citrus aurantium L. "Russian colon" has been analyzed by GC-MS. Twenty three components accounting to 94.38% of the total detected constituents were identified. The major ones were: linalool (49.90%), linalyl acetate (13.09%), α-terpineol (8.81%), geraniol (4.69%) and geranyl acetate (4.49%). Screening of the cytotoxic effect of the oil on two malignant cell lines of hepatic origin (HepG2), and breast tissue origin (MCF-7) was studied. The oil exhibited a moderate activity against HepG-2 cell line only.
Research Interests:
Phytochemical examination of corncob extracts led to the isolation of a new lignan identified as 7,7'-dihydroxy-3'-O-demethyl-4-methoxymatairesinol, together with seven known compounds, identified as β-sitosterol,... more
Phytochemical examination of corncob extracts led to the isolation of a new
lignan identified as 7,7'-dihydroxy-3'-O-demethyl-4-methoxymatairesinol, together with
seven known compounds, identified as β-sitosterol, β-sitosteryl-β-D-glucoside, 6β-hydroxycampest-
4-en-3-one, 5α,8α-epidioxyergosta-6,22-dien-3β-ol, tricin, kaempferol and
p-coumaric acid. The isolated compounds were identified by one and two-dimensional
NMR spectroscopies and mass spectrometry.
lignan identified as 7,7'-dihydroxy-3'-O-demethyl-4-methoxymatairesinol, together with
seven known compounds, identified as β-sitosterol, β-sitosteryl-β-D-glucoside, 6β-hydroxycampest-
4-en-3-one, 5α,8α-epidioxyergosta-6,22-dien-3β-ol, tricin, kaempferol and
p-coumaric acid. The isolated compounds were identified by one and two-dimensional
NMR spectroscopies and mass spectrometry.
Research Interests:
Microbial metabolism of danazol (17a-pregna-2,4-dien-20-yno[2,3-d]isoxazol-17b-ol) by Beauveria bassiana ATCC 7159 and Glyocladium viride ATCC 10097 afforded four metabolites. The isolated metabolites were identified by different... more
Microbial metabolism of danazol (17a-pregna-2,4-dien-20-yno[2,3-d]isoxazol-17b-ol) by Beauveria
bassiana ATCC 7159 and Glyocladium viride ATCC 10097 afforded four metabolites.
The isolated metabolites were identified by different spectroscopic techniques as 6b-
hydroxy danazol, which is a not yet reported danazol metabolite, 17b-hydroxy-17a-pregn-4-en-
20-yn-3-one (ethisterone) and 17b-hydroxy-2a-(hydroxymethyl)-17a-pregn-4-en-20-yn-3-one (2a-
hydroxymethyl ethisterone), which represent the major danazol metabolites detected in human urine.
The last metabolite, 6b,17b-dihydroxy-2-(hydroxymethyl)-17a-pregna-1,4-dien-20-yn-3-one, is also
a minor human metabolite, for which the NMR data are described here for the first time. The metabolites
were isolated in quantities that allowed their use for direct comparison in routine doping analysis.
bassiana ATCC 7159 and Glyocladium viride ATCC 10097 afforded four metabolites.
The isolated metabolites were identified by different spectroscopic techniques as 6b-
hydroxy danazol, which is a not yet reported danazol metabolite, 17b-hydroxy-17a-pregn-4-en-
20-yn-3-one (ethisterone) and 17b-hydroxy-2a-(hydroxymethyl)-17a-pregn-4-en-20-yn-3-one (2a-
hydroxymethyl ethisterone), which represent the major danazol metabolites detected in human urine.
The last metabolite, 6b,17b-dihydroxy-2-(hydroxymethyl)-17a-pregna-1,4-dien-20-yn-3-one, is also
a minor human metabolite, for which the NMR data are described here for the first time. The metabolites
were isolated in quantities that allowed their use for direct comparison in routine doping analysis.