Sustainable Living

UHPLC with QTOF-MS is widely used as a powerful tool for metabolomic analysis. This technology has recently been applied to the analysis of polyphenols in food and herb extracts. Sage (Salvia officinalis) and rosemary (Rosmarinus officinalis), belonging to the family Lamiaceae, are known for their potent antioxidant properties due to the presence of polyphenols. We have developed a sensitive and reproducible UHPLC-QTOF-MS/MS-based method for comprehensive phytochemical profiling and the identification and quantitation of specific polyphenolic compounds present in sage and rosemary leaves. The herbs were extracted ultrasonically using methanol as the solvent. In sage, rosmarinic acid (17 678.7±673.4 µg/g) and 12-methoxy carnosic acid (21 918.3±715.4 µg/g) were found in the highest concentrations among all polyphenols. In contrast, rosmarinic acid (14 311.0±636.4 µg/g), luteolin-3'-acetyl-O-glucuronide (1488.50±47.58 µg/g), and luteolin-7-O-glucuronide (1053.68±68.83 µg/g) were ob...

Rosmarinus officinalis is a potent antioxidant herb rich in polyphenols. Ultra-high-performance liquid chromatography, coupled with electrospray ionization and quadrupole-time of flight mass spectrometry (UHPLC-ESI-QTOF-MS), enables an exhaustive, full-spectrum analysis of the molecular constituents of natural products. The study aimed to develop a rapid UHPLC method to contribute new insights into the phytochemical composition of rosemary and to assess the performance of nine different procedures for extraction. These include fresh tissue homogenization, fresh and dry leaf decoction, and their respective fermentation, Soxhlet extraction, and sonication using water and methanol. Different extraction methods were found to recover quite different groups of polyphenols within 11 min during 20 min of analysis. Soxhlet extraction, yielded very high concentrations of rosmarinic acid (33,491.33 ± 86.29 µg/g), luteolin-7-O-glucoside (209.95 ± 8.78 µg/g), carnosic acid (2915.40 ± 33.23 µg/g)...

Sage (Salvia officinalis L.) is an important medicinal and aromatic herb, used as a raw material for various perfumery, pharmaceutical, food and cosmetics industries. However in sage, seed germination is a problem of great concern, as the germination percentage is less than 50 % due to dormancy, resulting lack of synchronized crop establishment. Hence an investigation was carried out to break the seed dormancy for uniform and early seed germination by using low cost organic seed priming techniques. Ten pre-sowing organic seed treatments were imposed by using locally available resources such as; water soaking (cold and hot), cow urine (5, 10, 15 and 20 %), cow dung slurry, buttermilk, compost tea and Agnihotra ash along with an untreated control. Among the different treatments, seed germination percentage was significantly high (79.67) in cow dung slurry treatment followed by cow urine at 5 per cent (72.33) as compared to control (35.33 %). Cow dung slurry treatment also recorded uni...

Sage (Salvia officinalis L.) is an important medicinal and aromatic herb, used as a raw material for various perfumery, pharmaceutical, food, and cosmetics industries. S. officinalis belonging to the family Lamiaceae is commercially cultivated for the essential oil and is grown throughout Europe and the United States, and in parts of Turkey, Yugoslavia, and Canada. Leaves are highly aromatic, used for extraction of essential oil, which contains more than 49 aromatic components. The principal components in the sage oil are 1, 8-cineole, camphor, α-thujone, β-thujone, α-humulene, rosmarinic acid, and quercetin. Traditionally garden sage has been used for the treatment of a multitude of ailments such as localized pain, rheumatism, convulsion, arthritis, vertigo, diarrhea, sclerosis, respiratory, metabolic and mental disorders. As the herbal medicines gaining importance in recent years, usage and systematic documentation of such herb are found essential. Thus an effort has been made to ...

UHPLC with QTOF-MS is widely used as a powerful tool for metabolomic analysis. This technology has recently been applied to the analysis of polyphenols in food and herb extracts. Sage (Salvia officinalis) and rosemary (Rosmarinus officinalis), belonging to the family Lamiaceae, are known for their potent antioxidant properties due to the presence of polyphenols. We have developed a sensitive and reproducible UHPLC-QTOF-MS/MS-based method for comprehensive phytochemical profiling and the identification and quantitation of specific polyphenolic compounds present in sage and rosemary leaves. The herbs were extracted ultrasonically using methanol as the solvent. In sage, rosmarinic acid (17 678.7±673.4 µg/g) and 12-methoxy carnosic acid (21 918.3±715.4 µg/g) were found in the highest concentrations among all polyphenols. In contrast, rosmarinic acid (14 311.0±636.4 µg/g), luteolin-3'-acetyl-O-glucuronide (1488.50±47.58 µg/g), and luteolin-7-O-glucuronide (1053.68±68.83 µg/g) were ob...

Rosmarinus officinalis is a potent antioxidant herb rich in polyphenols. Ultra-high-performance liquid chromatography, coupled with electrospray ionization and quadrupole-time of flight mass spectrometry (UHPLC-ESI-QTOF-MS), enables an exhaustive, full-spectrum analysis of the molecular constituents of natural products. The study aimed to develop a rapid UHPLC method to contribute new insights into the phytochemical composition of rosemary and to assess the performance of nine different procedures for extraction. These include fresh tissue homogenization, fresh and dry leaf decoction, and their respective fermentation, Soxhlet extraction, and sonication using water and methanol. Different extraction methods were found to recover quite different groups of polyphenols within 11 min during 20 min of analysis. Soxhlet extraction, yielded very high concentrations of rosmarinic acid (33,491.33 ± 86.29 µg/g), luteolin-7-O-glucoside (209.95 ± 8.78 µg/g), carnosic acid (2915.40 ± 33.23 µg/g)...

Sage (Salvia officinalis L.) is an important medicinal and aromatic herb, used as a raw material for various perfumery, pharmaceutical, food and cosmetics industries. However in sage, seed germination is a problem of great concern, as the germination percentage is less than 50 % due to dormancy, resulting lack of synchronized crop establishment. Hence an investigation was carried out to break the seed dormancy for uniform and early seed germination by using low cost organic seed priming techniques. Ten pre-sowing organic seed treatments were imposed by using locally available resources such as; water soaking (cold and hot), cow urine (5, 10, 15 and 20 %), cow dung slurry, buttermilk, compost tea and Agnihotra ash along with an untreated control. Among the different treatments, seed germination percentage was significantly high (79.67) in cow dung slurry treatment followed by cow urine at 5 per cent (72.33) as compared to control (35.33 %). Cow dung slurry treatment also recorded uni...

Sage (Salvia officinalis L.) is an important medicinal and aromatic herb, used as a raw material for various perfumery, pharmaceutical, food, and cosmetics industries. S. officinalis belonging to the family Lamiaceae is commercially cultivated for the essential oil and is grown throughout Europe and the United States, and in parts of Turkey, Yugoslavia, and Canada. Leaves are highly aromatic, used for extraction of essential oil, which contains more than 49 aromatic components. The principal components in the sage oil are 1, 8-cineole, camphor, α-thujone, β-thujone, α-humulene, rosmarinic acid, and quercetin. Traditionally garden sage has been used for the treatment of a multitude of ailments such as localized pain, rheumatism, convulsion, arthritis, vertigo, diarrhea, sclerosis, respiratory, metabolic and mental disorders. As the herbal medicines gaining importance in recent years, usage and systematic documentation of such herb are found essential. Thus an effort has been made to ...