eman elsharkawy
Northern Borders University, Chemistry, Faculty Member
- Dr Eman Rmadan ElsharkawyAss.prof of Organic chemistryNorthern Border universityedit
This study reports the biochemical profile and in vitro biological activities of the aerial part of two shrubs: Halocnemum strobilaceum and Suaeda fruticosa, a halophytes species native to saline habitats. The biomass was evaluated by... more
This study reports the biochemical profile and in vitro biological activities of the aerial part of two shrubs: Halocnemum strobilaceum and Suaeda fruticosa, a halophytes species native to saline habitats. The biomass was evaluated by determining its physiological properties and approximate composition. Hydro-methanolic extracts from Halocnemum strobilaceum and Suaeda fruticosa have been investigated for the inhibition of bacterial growth, the protection of proteins (albumin) from denaturation, and cytotoxicity to hepatocellular carcinomas (Huh-7 and HepG2). Their antioxidant activity was evaluated by five tests, including one that examined their ability to inhibit hydrogen peroxide (H2O2)-induced hemolysis. The profile of their phenolic compounds was also determined. These two euhalophytes had a high moisture content, high levels of photosynthetic pigments, elevated levels of ash and protein, low oxidative damage indices, MDA (Malondialdehyde) and proline, and low lipids levels. Th...
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Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histopathological studies of methanol extract of Deverra tortuosa in streptozotocin-induced diabetic rats were investigated in vivo. A single... more
Herbal plants represent a new source of hypoglycemic antidiabetic drugs; haematological and histopathological studies of methanol extract of Deverra tortuosa in streptozotocin-induced diabetic rats were investigated in vivo. A single intraperitoneal injection of 160 mg/kg bodyweights of streptozotocin was used to cause diabetes. Blood glucose levels were tested with an AccuCheck Advantage II glucometer and blood glucose test strips. After diabetes was confirmed, animals were orally treated with the extract, metformin, and insulin according to the experimental design. After extract therapy, histological alterations in the pancreas of diabetic rats were investigated. When compared to a control group, daily oral administration of D. tortuosa extract (300 mg/kg body weight) plus metformin (100 mg/kg) had a positive effect on blood glucose levels as well as showed an increased number of white blood cells (WBCs) and red blood cells (RBCs). The treatment with the extract for two weeks show...
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Nanotechnology nowadays has good potential application in many fields, especially in agriculture and industries. This study investigated the allelopathic effects of aqueous extract of Pulicaria undulate and nanoparticle synthesis by... more
Nanotechnology nowadays has good potential application in many fields, especially in agriculture and industries. This study investigated the allelopathic effects of aqueous extract of Pulicaria undulate and nanoparticle synthesis by eco-friendly method from silver nitrate and water extract of Pulicaria undulate on seed germination of two weeds species. Aqueous extract and silver Nanoparticles plant extract (AgNPs) had an inhibitory effect on seed germination of common (Lepidium sativum, and Trifolium repens) as compared with control. Lpidum stavium seed germination was inhibited by nanoparticle and water plant extract at 100% concentration at the end of 10 day incubation period, at low concentration 25% of aqueous extract and AgNPs the germination ratio of L. sativium reached (30% and 5%) where for Trifolium repens the germination ratio were (50% and 0% respectively). Nanoparticle are green synthesis by simple methods, using the aqueous extract of pulclaria leave and silver nitrate,...
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Drought stress and drastic condition of northern region especial high temperature in summer season affect widely on the chemical component of plant and its biological actively plant grow in these condition adapt to this stress by... more
Drought stress and drastic condition of northern region especial high temperature in summer season affect widely on the chemical component of plant and its biological actively plant grow in these condition adapt to this stress by accumulation different compound, thugia it is tree grow in garden and farms, when it grows in desert habitat show different content, Gc-Ms analysis of the terpenoid fraction of the plant where it is best fraction given high antimicrobial and Larvicidal activity was done and show the presence of many promising diterpene compounds, Ferruginol, Torulosol, dihydro, 18-Oxo-kauran-17-yl acetate and Andrographolide. The antibacterial testing showed that, the tested gram-positive bacteria exhibited good susceptibility to the terpenoid fraction of methanol extract, which was Staphylococcus epidermidis and Staphylococcus aureus, recorded 13.0±0.7 and 11.0±0.0 mm inhibition zones, respectively. Indicating promising antibacterial activity against the gram-positive path...
Two known polyphenols named apigenin 7-O-β-d-glucopyranoside (S1) and querctine-3-O-glucoside (S2), along with another two new compounds apigenin 4'-geranyl-8-glucopyranosyl-7-O-α-glucopyranoside (S3) and apigenin... more
Two known polyphenols named apigenin 7-O-β-d-glucopyranoside (S1) and querctine-3-O-glucoside (S2), along with another two new compounds apigenin 4'-geranyl-8-glucopyranosyl-7-O-α-glucopyranoside (S3) and apigenin 4'-pernyl-8-glucopyranosyl -7-O-α-glucopyranoside (S4), were isolated from the leaves of Cupressus sempervirens. Structure elucidation of the isolated polyphenols was established on the basis of detailed spectroscopic analysis like 1D and 2D NMR analyses including 1H NMR, 13C NMR, COSY, DEPT, HMQC, UV, and Electron Spray Ionization Mass Spectroscopy (ESI-MS). Density Functional Theory (DFT) of computational, Petra/Osiris/Molinspiration (POM), and docking analyses methods were applied in the structural validation of new isolated compounds. The isolated compounds S1-S4 showed significant cytotoxicity against human hepatocellular liver carcinoma HepG2 cells, MCF-7, HC116 and A549.
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In this study, Thymus numidicus Poir. plant material was collected from two different locations in north-western Tunisia and the aerial parts essential oils (EOs) were extracted via hydro-distillation. Gas chromatography coupled to mass... more
In this study, Thymus numidicus Poir. plant material was collected from two different locations in north-western Tunisia and the aerial parts essential oils (EOs) were extracted via hydro-distillation. Gas chromatography coupled to mass spectrometry (GC-MS) and flame ionisation detection (GC-FID) were used for the qualitative analysis and quantification of the volatile constituents. Thymol (50.1-52.8%) was identified as the main compound of both EOs. To evaluate the potential application of the EOs as antifungal agents, the in vitro inhibitory effects were tested against six fungal strains; a strong antifungal activity of one sample was observed (MIC = 40-400 µg/mL). The in vitro antiproliferative activity was investigated on two human cancer cell lines, i.e. the colonic (HCT116) and breast adenocarcinoma (MCF7) using the colourimetric MTT assay. Again, the same sample demonstrated to possess good antiproliferative activity against both cancer cell lines, with IC50 values of 26.9 an...
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As part of our ongoing collaborative effort to discover the anticancer activity of the phenolics isolated from terrestrial plant sources, the EtOH extract of the aerial parts of the Egyptian medicinal plant Diplotaxis harra (Forssk.)... more
As part of our ongoing collaborative effort to discover the anticancer activity of the phenolics isolated from terrestrial plant sources, the EtOH extract of the aerial parts of the Egyptian medicinal plant Diplotaxis harra (Forssk.) Boiss. was in vitro investigated for cytotoxicity against HCT116, HepG2 and MCF-7 cell lines, and resulted with IC50 = 4.65, 12.60 and 17.90 µg/ml, respectively. Doxorubicin (+ve control) showed in vitro cytotoxic activity with IC50 = 3.64, 4.57 and 2.97 µg/ml, respectively. The phenolic-rich fraction of the EtOH extract was subjected to further fractionation, which led to the isolation of five flavonoids; identified as quercetin, quercetin 3-O-β-glucoside, isorhamnetin 7-O-β-glucoside, apigenin 3-O-β-rhamnoside and kaempferol 3-O-β-glucoside, according to its’ spectral data and comparison with the literature. Furthermore, the isolated flavonoids showed in vitro cytotoxicity against HCT116 cell line with IC50 = 20.1, 24.3, 22.8, 23.4 and 41.9 µg/ml as d...
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Medicinal herbs are also significant source of synthetic and herbal drugs. So far, pharmaceutical companies have screened more than 25,000 plants for anti-cancer drugs. So we done to discover the anticancer activity of the terpinoid... more
Medicinal herbs are also significant source of synthetic and herbal drugs. So far, pharmaceutical companies have screened more than 25,000 plants for anti-cancer drugs. So we done to discover the anticancer activity of the terpinoid compound isolated from terrestrial Saudi plants, the MeOH extract of the aerial parts of the plant lactuca steriolla family Asteraceae was in vitro investigated for cytotoxicity against HCT116, A549, HepG2 and MCF-7 cell lines, and resulted in vitro show high cytotoxic activity against breast cancer with for MeOH extract and good cytotoxicity against liver cancer of hexane extract, respectively, Organic extracts , were fractionated through classic chromatography. The steroids stigmasterol acetate, β-sitosterol and campesterol were identified in hexane extract. Triterpenes, germincol, lupeol, α-amyrin, β-amyrin, olean 18-ene, lupeol acetate were isolated from methanol extract. Steroids and 14b-perganane and 4- piprdenone were isolated from methanol extrac...
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Cannabis sativa is a well-known plant that has been recognized for its benefits since ancient times by several medicinal systems, including those of China, India, Greece, and Egypt. Although C. sativa is one of the most investigated... more
Cannabis sativa is a well-known plant that has been recognized for its benefits since ancient times by several medicinal systems, including those of China, India, Greece, and Egypt. Although C. sativa is one of the most investigated medicinal plants in the world, it faces some of the greatest controversies surrounding its legalization and use as a medication. C. sativa contains several hundred phytoconstituents, including the infamous “cannabinoids”. It is necessary to properly understand the medicinal importance of these phytochemicals and spread awareness among the countries where cannabis is still facing legal obstacles. The current review focuses on the most recent literature pertaining to various applications of cannabinoids, with a special focus on the medicinal aspect of these phytochemicals. Peer-reviewed articles focusing on the importance of cannabis and cannabinoids are the target of this review. Articles were selected based on the relevance to the general scope of the wo...
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Synthesis of nanoparticles is a fast-growing area of interest in the current development in science and technology. Nanoparticles are also used in biomedical applications. Green synthesis of nanoparticles is an environmental friendly and... more
Synthesis of nanoparticles is a fast-growing area of interest in the current development in science and technology. Nanoparticles are also used in biomedical applications. Green synthesis of nanoparticles is an environmental friendly and cost-effective technique. Trillium govanianum Wall. Ex. Royle crude extract was used for the eco-friendly genesis of silver nanoparticles (AgNPs). Aromatic amines were the functional groups involved in the bio-fabrication and synthesis of the AgNPs. The production of AgNPs was established by the appearance of brown color. The manufactured AgNPs were characterized by UV-Vis spectrophotometer, X-ray diffractometer, and FTIR spectrophotometer. AgNPs were face-centered cubic in nature with an average size of 9.99 nm. The produced AgNPs (18 µL disc−1) showed substantial antibacterial (53.74, 52.75, 51.61, 43.00, 36.84, and 36.84%) and antifungal (54.05, 42.11, 41.10, 40.85, 30.55, and 29.73%) potential against the tested bacterial (X. campestris, P. aeru...
Honokiol ((3',5-di-(2-propenyl)-1,1'-biphenyl-2,2'-diol), a lignan, is a promising antitumor compound, having exerted activity against a number of human cancer cell lines. Honokiol has inhibitory role on the proliferation,... more
Honokiol ((3',5-di-(2-propenyl)-1,1'-biphenyl-2,2'-diol), a lignan, is a promising antitumor compound, having exerted activity against a number of human cancer cell lines. Honokiol has inhibitory role on the proliferation, invasion and survival of cancer cells in in vitro as well as in vivo studies. It interferes with signaling pathways components in order to elicit the anticancer effect. In present review, the published data on the efficacy of honokiol against various cancer cell lines and tumor-bearing animal models has been presented and discussed. Honokiol lowers the expression of pluripotency-factors, the formation of mammosphere, P-glycoprotein expression, receptor CXCR4 level, c-FLIP, steroid receptor coactivator-3 (SRC-3), Twist1, matrix metalloproteinases, class I histone deacetylases, H3K27 methyltransferase among numerous other anticancer functions. It increases bone morphogenetic protein 7 (BMP7), Bax protein, among others. It does so by interfering with the ...
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Medicinal herbs are also significant source of synthetic and herbal drugs. So far, pharmaceutical companies have screened more than 25,000 plants for anti-cancer drugs. So we done to discover the anticancer activity of the terpinoid... more
Medicinal herbs are also significant source of synthetic and herbal drugs. So far, pharmaceutical companies have screened more than 25,000 plants for anti-cancer drugs. So we done to discover the anticancer activity of the terpinoid compound isolated from terrestrial Saudi plants, the MeOH extract of the aerial parts of the plant lactuca steriolla family Asteraceae was in vitro investigated for cytotoxicity against HCT116, A549, HepG2 and MCF-7 cell lines, and resulted in vitro show high cytotoxic activity against breast cancer with for MeOH extract and good cytotoxicity against liver cancer of hexane extract, respectively, Organic extracts , were fractionated through classic chromatography. The steroids stigmasterol acetate, β-sitosterol and campesterol were identified in hexane extract. Triterpenes, germincol, lupeol, α-amyrin, β-amyrin, olean 18-ene, lupeol acetate were isolated from methanol extract. Steroids and 14b-perganane and 4- piprdenone were isolated from methanol extrac...
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Abstract The volatile components of the aerial parts of Santolina chamaecyparissus, an aromatic plant of North region, Saudi Arabia, were analyzed in two season by GC and GC-MS. Among the 39 compounds identified, major components were... more
Abstract The volatile components of the aerial parts of Santolina chamaecyparissus, an aromatic plant of North region, Saudi Arabia, were analyzed in two season by GC and GC-MS. Among the 39 compounds identified, major components were Curcumene, alpha-terpinol, p-cymene, 1,8-cineole and caryophllene oxide. Quantitative variations have been characterized following the season. Oil extract of the aerial parts of the medicinal plant Santolina, was in vitro investigated for cytotoxicity against A549, HCT116, HepG2 and MCF-7 cell lines, and resulted show high cytotoxic activity against HepG2 with (97.7 %), but resistance to other cell lines, The percentages of inhibition related to the reference drug (doxorubicin), as long as people are looking to alternative forms of medicine and relaxation such as aromatherapy in this review we confirm the use of one of these medicinal plant as antitumor agent.
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The study was designed to investigate the anti-inflammatory and composition of essential oil of two plant family Asteraceae, Achillea fragrantissima and Lactuca serriola growing under dry desert condition. The anti-inflammatory effect of... more
The study was designed to investigate the anti-inflammatory and composition of essential oil of two plant family Asteraceae, Achillea fragrantissima and Lactuca serriola growing under dry desert condition. The anti-inflammatory effect of volatile oil extracted by hydrodistillation of plants was studied using carrageenan induced paw edema. Essential oil (100 mg/kg) and (200 mg/kg) were tested the two plant show high inhibition after 4 h, concentration (200 mg/kg) show high inhibition than (100 mg/kg) after 4 h. Sesqui sabinene hydrate, Azuline and α-Bisabolol are the main constituents of the volatile oil were investigated by capillary GC (gas chromatography) and GC-MS (gas chromatography-mass spectrometry). The discussion shows the role of chemical compound azulene in inflammatory inhibition.
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Five main flavonoid glycosides were isolated from the ethanolic extract of Diplotaxis harra (Cruciferae), and were identified as quercetin, isorhamnetin 3‐rhamnoside, isorhamnetin 3‐o‐rutinoside, isorhamnetin 3‐glucosyl‐4`‐rhamnoside and... more
Five main flavonoid glycosides were isolated from the ethanolic extract of Diplotaxis harra (Cruciferae), and were identified as quercetin, isorhamnetin 3‐rhamnoside, isorhamnetin 3‐o‐rutinoside, isorhamnetin 3‐glucosyl‐4`‐rhamnoside and isorhamnetin 3‐o‐β‐glucoside. These compounds were identified according to their Rf values, partial and complete acid hydrolysis, UV, 1H NMR, 13C NMR and 2D NMR (HSQC, HMBC) spectroscopy. The alcoholic extract of plant was evaluated against some bacterial strains which showed moderate antibacterial activity, while petroleum ether extract doesn't show any activity.
As part of our ongoing collaborative effort to discover the anticancer activity of the phenolics isolated from terrestrial plant sources, the EtOH extract of the aerial parts of the Egyptian medicinal plant Diplotaxis harra (Forssk.)... more
As part of our ongoing collaborative effort to discover the anticancer activity of the phenolics isolated from terrestrial plant sources, the EtOH extract of the aerial parts of the Egyptian medicinal plant Diplotaxis harra (Forssk.) Boiss. was in vitro investigated for cytotoxicity against HCT116, HepG2 and MCF-7 cell lines, and resulted with IC 50 = 4.65, 12.60 and 17.90 µg/ml, respectively. Doxorubicin (+ve control) showed in vitro cytotoxic activity with IC 50 = 3.64, 4.57 and 2.97 µg/ml, respectively. The phenolic-rich fraction of the EtOH extract was subjected to further fractionation, which led to the isolation of five flavonoids; identified as quercetin, quercetin 3-O-β-glucoside, isorhamnetin 7-O-β-glucoside, apigenin 3-O-β-rhamnoside and kaempferol 3-O-β-glucoside, according to its' spectral data and comparison with the literature. Furthermore, the isolated flavonoids showed in vitro cytotoxicity against HCT116 cell line with IC 50 = 20.1, 24.3, 22.8, 23.4 and 41.9 µg/...
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The current study investigated the levels of multi pesticide residues in the highly consumed types of Nile fish in Egypt: tilapia and cat fish. A total of 50 Nile tilapia (Oreochromis niloticus) and 50 African catfish (Clarias gariepinus)... more
The current study investigated the levels of multi pesticide residues in the highly consumed types of Nile fish in Egypt: tilapia and cat fish. A total of 50 Nile tilapia (Oreochromis niloticus) and 50 African catfish (Clarias gariepinus) were collected from two areas in Assiut city, where most industries are situated and where agricultural activities and raising of farm animals are the main activities. In the first area, Elwasta, there is an electrical power station, and the second area, Mankbad, there is a cement factory. Fish samples were analyzed by High Resolution Gas Chromatography/Mass Spectrometry. Average pesticide residue concentrations±SE in muscle of tilapia and catfish (n = 10 pooled samples with five fish each) were determined. The results indicated the presence of different types of organophosphorous (OPs), organochlorine (OC), polychlorinated biphenyles (PCBs), hexachlorobenzene (HCB) and trifluralin pesticides in Elwasta and Mankbad in varying degrees. Diazenon was ...
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The chloroform fraction of methanol (MeOH) extract of the aerial parts of Launaea mucronata was in vitro investigated for cytotoxicity against HCT116, HepG2 and MCF-7 cell lines, and resulted with IC50 = 20.0, 18.6 and 14.30 μg/mL,... more
The chloroform fraction of methanol (MeOH) extract of the aerial parts of Launaea mucronata was in vitro investigated for cytotoxicity against HCT116, HepG2 and MCF-7 cell lines, and resulted with IC50 = 20.0, 18.6 and 14.30 μg/mL, respectively. The chloroform fraction of the MeOH extract was subjected to further fractionation, which led to the isolation of two new coumarin compounds (6-isobutyl coumarin and 6-isobutyl-7-methyl- coumarin). The structures of the new compounds were elucidated by high field 1D and 2D NMR and ESI-MS spectroscopies. Low polar fractions revealed the isolation of other known triterpene compounds which were identified according to its spectral data and comparison with the literature. New coumarin compounds show high cytotoxicity against MCF-7, HCT116 and HepG2 cell lines.
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ABSTRACT A new flavonoid kaempferol-7,8-diglucoside was isolated for the first time along with two known flavonoid compounds, kaempferol and quercetin, from the methanol extract of Farsetia aegyptia Turra by chromatographic techniques.... more
ABSTRACT A new flavonoid kaempferol-7,8-diglucoside was isolated for the first time along with two known flavonoid compounds, kaempferol and quercetin, from the methanol extract of Farsetia aegyptia Turra by chromatographic techniques. Two coumarin compounds were isolated and identified from the plant extract. The plant extract contains phenol, catechin, p-hydroxybenzoic acid, gallic acid, pyrogallic acid, p-coumaric acid, and coumarin compounds, which were identified by authentic samples using HPLC. The new compound and alcoholic extract of the plant were evaluated against some bacterial and fungal strains. They showed maximum inhibition against Klebsiella pneumonia. Moderate effects were observed to Aspergillus niger, while the extract did not show activity against Candida albicans.