Fluorosis, caused by a chronic exposure to fluoride is a worldwide health problem known to affect... more Fluorosis, caused by a chronic exposure to fluoride is a worldwide health problem known to affect various organ systems. The present study was aimed at investigating the effect(s) of extracts of seasonal fruits (Indian goose berry, star fruit, guava and wood apple) on angiotensin-converting enzyme (ACE) activity in the lung extracts exposed to different concentrations of fluoride. The lung extracts showed an increasing ACE activity with increasing concentrations of fluoride (2.5-10ppm) with highest ACE activity at 10ppm of fluoride. A reduction in the ACE activity was observed when the fluoride exposed (10ppm) lung extracts were incubated with fruit extracts; wood apple extract exerted a maximum ACE inhibitory activity (28.9%) followed by star fruit, guava and goose berry (20.1 %,19% and 15.5% respectively). A phytochemical analysis and the antioxidant potential of the fruits indicated that the fruits have a high antioxidant potential that correlated well with phytochemical content (phenols, flavonoids, saponins and ascorbic acid) of the fruits. It is suggested that the consumption of these seasonal fruits could be useful in regulating the ACE activity in fluoride endemic areas.
To examine its antidiabetic potential in fluoride (F) intoxicated rats, the
anti-diabetic drug gl... more To examine its antidiabetic potential in fluoride (F) intoxicated rats, the anti-diabetic drug glibenclamide was administered for 4 weeks to diabetic rats and to diabetic rats exposed to 100 mg NaF/L in the drinking water. In the F treated rats there was a significant reduction in plasma glucose, plasma and hepatic total lipids, cholesterol, triglycerides and plasma low-density lipoproteins (LDL), VLDLcholesterol, and atherogenic index accompanied by significant increases in HDLcholesterol, FRAP (ferric reducing ability of plasma) and protein content. Furthermore, significant decreases in SGOT (serum glutamate oxaloacetate transaminase), SGPT (serum glutamate pyruvate transaminase), alkaline and acid phosphatase (ALP and ACP), and glucose-6-phosphatase (G-6-Pase) were observed in these F-treated animals. In addition, administration of the drug decreased hepatic and renal lipid peroxidation with a concomitant increase in total ascorbic acid (TAA), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPX), and FRAP levels in the F-treated animals. It is proposed that glibenclamide acts at two levels: (i) at pancreatic islets for increased release of insulin from surviving β cells, (ii) at the target sites, e.g., hepatic tissue to improve glucose uptake leading to an improvement in the activities of hepatic and renal TAA, SOD, GSH, GPX, and reduction in lipid peroxidation. Glibenclamide may therefore be useful for treatment of diabetes in F endemic areas
Fluorosis, caused by a chronic exposure to fluoride is a worldwide health problem known to affect... more Fluorosis, caused by a chronic exposure to fluoride is a worldwide health problem known to affect various organ systems. The present study was aimed at investigating the effect(s) of extracts of seasonal fruits (Indian goose berry, star fruit, guava and wood apple) on angiotensin-converting enzyme (ACE) activity in the lung extracts exposed to different concentrations of fluoride. The lung extracts showed an increasing ACE activity with increasing concentrations of fluoride (2.5-10ppm) with highest ACE activity at 10ppm of fluoride. A reduction in the ACE activity was observed when the fluoride exposed (10ppm) lung extracts were incubated with fruit extracts; wood apple extract exerted a maximum ACE inhibitory activity (28.9%) followed by star fruit, guava and goose berry (20.1 %,19% and 15.5% respectively). A phytochemical analysis and the antioxidant potential of the fruits indicated that the fruits have a high antioxidant potential that correlated well with phytochemical content (phenols, flavonoids, saponins and ascorbic acid) of the fruits. It is suggested that the consumption of these seasonal fruits could be useful in regulating the ACE activity in fluoride endemic areas.
To examine its antidiabetic potential in fluoride (F) intoxicated rats, the
anti-diabetic drug gl... more To examine its antidiabetic potential in fluoride (F) intoxicated rats, the anti-diabetic drug glibenclamide was administered for 4 weeks to diabetic rats and to diabetic rats exposed to 100 mg NaF/L in the drinking water. In the F treated rats there was a significant reduction in plasma glucose, plasma and hepatic total lipids, cholesterol, triglycerides and plasma low-density lipoproteins (LDL), VLDLcholesterol, and atherogenic index accompanied by significant increases in HDLcholesterol, FRAP (ferric reducing ability of plasma) and protein content. Furthermore, significant decreases in SGOT (serum glutamate oxaloacetate transaminase), SGPT (serum glutamate pyruvate transaminase), alkaline and acid phosphatase (ALP and ACP), and glucose-6-phosphatase (G-6-Pase) were observed in these F-treated animals. In addition, administration of the drug decreased hepatic and renal lipid peroxidation with a concomitant increase in total ascorbic acid (TAA), superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase (GPX), and FRAP levels in the F-treated animals. It is proposed that glibenclamide acts at two levels: (i) at pancreatic islets for increased release of insulin from surviving β cells, (ii) at the target sites, e.g., hepatic tissue to improve glucose uptake leading to an improvement in the activities of hepatic and renal TAA, SOD, GSH, GPX, and reduction in lipid peroxidation. Glibenclamide may therefore be useful for treatment of diabetes in F endemic areas
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Papers by Krutika Bhole
anti-diabetic drug glibenclamide was administered for 4 weeks to diabetic rats and to
diabetic rats exposed to 100 mg NaF/L in the drinking water. In the F treated rats there
was a significant reduction in plasma glucose, plasma and hepatic total lipids,
cholesterol, triglycerides and plasma low-density lipoproteins (LDL), VLDLcholesterol,
and atherogenic index accompanied by significant increases in HDLcholesterol,
FRAP (ferric reducing ability of plasma) and protein content.
Furthermore, significant decreases in SGOT (serum glutamate oxaloacetate
transaminase), SGPT (serum glutamate pyruvate transaminase), alkaline and acid
phosphatase (ALP and ACP), and glucose-6-phosphatase (G-6-Pase) were observed
in these F-treated animals. In addition, administration of the drug decreased hepatic
and renal lipid peroxidation with a concomitant increase in total ascorbic acid (TAA),
superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase
(GPX), and FRAP levels in the F-treated animals. It is proposed that glibenclamide
acts at two levels: (i) at pancreatic islets for increased release of insulin from
surviving β cells, (ii) at the target sites, e.g., hepatic tissue to improve glucose uptake
leading to an improvement in the activities of hepatic and renal TAA, SOD, GSH, GPX,
and reduction in lipid peroxidation. Glibenclamide may therefore be useful for
treatment of diabetes in F endemic areas
anti-diabetic drug glibenclamide was administered for 4 weeks to diabetic rats and to
diabetic rats exposed to 100 mg NaF/L in the drinking water. In the F treated rats there
was a significant reduction in plasma glucose, plasma and hepatic total lipids,
cholesterol, triglycerides and plasma low-density lipoproteins (LDL), VLDLcholesterol,
and atherogenic index accompanied by significant increases in HDLcholesterol,
FRAP (ferric reducing ability of plasma) and protein content.
Furthermore, significant decreases in SGOT (serum glutamate oxaloacetate
transaminase), SGPT (serum glutamate pyruvate transaminase), alkaline and acid
phosphatase (ALP and ACP), and glucose-6-phosphatase (G-6-Pase) were observed
in these F-treated animals. In addition, administration of the drug decreased hepatic
and renal lipid peroxidation with a concomitant increase in total ascorbic acid (TAA),
superoxide dismutase (SOD), reduced glutathione (GSH), glutathione peroxidase
(GPX), and FRAP levels in the F-treated animals. It is proposed that glibenclamide
acts at two levels: (i) at pancreatic islets for increased release of insulin from
surviving β cells, (ii) at the target sites, e.g., hepatic tissue to improve glucose uptake
leading to an improvement in the activities of hepatic and renal TAA, SOD, GSH, GPX,
and reduction in lipid peroxidation. Glibenclamide may therefore be useful for
treatment of diabetes in F endemic areas