Background and Aim: The use of drugs as a therapy for traumatic ulcers may lead to drug resistanc... more Background and Aim: The use of drugs as a therapy for traumatic ulcers may lead to drug resistance and other side effects. Lactobacillus casei Shirota can affect the number of fibroblasts and blood vessels in wound healing. The aim of this study was to investigate the difference in the number of fibroblast cells and blood vessels after the topical and systemic administration of L. casei Shirota probiotics in Wistar rats with traumatic ulcer. Materials and Methods: Overall, 36 healthy male Wistar rats aged 2-3 months old and weighing 175-250 g in body weight were used as a sample. Traumatic ulcer was made on the labial fornix incisive inferior. The subject rats were divided into groups: (1) A control group over 3 days, (2) a group that used distilled water over 7 days, (3) a group that underwent topical treatment over 3 days, (4) a group that used probiotics administered topically over 7 days, (5) a group that underwent systemic treatment over 3 days, and (6) a group that took oral p...
Objective Stem cells of human exfoliated deciduous teeth (SHED) metabolites are secreted molecule... more Objective Stem cells of human exfoliated deciduous teeth (SHED) metabolites are secreted molecules from SHED, namely cytokines, chemokines, and growth factors. The metabolite can be used in various regenerative therapy based on cell-free immunomodulatory potential effects, like interleukin 10 (IL-10) and LL37. This molecule can stimulate with epigallocatechin gallate (EGCG) and α-mangosteen and has been proven to have anti-inflammatory and antibacterial effects. This study aimed to identify the effect of EGCG and α-mangosteen to SHED metabolite, called SHED-IL10 and SHED-LL37, from six passages to obtain the optimum stimulation and able to use as periodontitis regeneration treatment. Materials and Methods The six different passages of SHED were prepared in Dulbecco's modified Eagle medium and added with EGCG 80% (10 μM), EGCG 95% (10 μM), or α-mangosteen (10 μM). After a 24 hours incubation, each passage was measured with the metabolite concentration, SHED-IL10 and SHED-LL37, wi...
Background: Periodontitis is a progressive destructive periodontal disease. The prevalence of per... more Background: Periodontitis is a progressive destructive periodontal disease. The prevalence of periodontal disease in Indonesia reaches 74.1% and mostly occurs in the productive age group. Most of the periodontopathogenic bacteria are gram-negative bacteria and have endotoxin in the form of lipopolysaccharide (LPS), which can penetrate the periodontal tissue and induce an inflammatory response. In inflammatory conditions, osteoclastic activity is higher than osteoblastic activity, which causes bone destruction. This results in an imbalance between osteoclast-induced bone resorption and osteoblast-induced bone formation. The current preferred treatment for periodontitis is scaling root planning (SRP), but this therapy cannot repair the damaged periodontal tissue caused by periodontitis. Purpose: To describe the possibility of using a mucoadhesive gingival patch with Epigallocatechin-3-gallate (EGCG) green tea (Camellia sinensis) as alternative adjunct therapy for periodontal disease. ...
Background: Cancer is a major cause of death worldwide. One of the most common forms of cancer is... more Background: Cancer is a major cause of death worldwide. One of the most common forms of cancer is oral cancer, which can occur due to exposure to carcinogenic factors, such as tobacco cigarettes, alcohol, betel-nut chewing, ultraviolet rays or human papillomavirus infection. Physical exercise is known to have many benefits and can contribute to reducing the risk of cancer, minimising the side-effects of treatment and increasing the curative effect of cancer treatment. Purpose: This study aimed to explain the role of exercise as a method to reduce oral cancer risk. Reviews: Studies examining the impact of exercise on reducing oral cancer risk are currently limited due to a lack of research on this subject. However, according to several laboratory experimental research studies on Mus musculus test subjects, moderate-intensity exercise contributes to suppressing the proliferation and development of oral squamous epithelial cells, which can subsequently become cancer cells. Exercise can...
Objective Topical application of ambonese banana (Musa paradisiaca var. sapientum (L.) kuntze) st... more Objective Topical application of ambonese banana (Musa paradisiaca var. sapientum (L.) kuntze) stem sap gel (GEGPA) on the socket wound area showed an increase in the expression of platelet-derived growth factor-BB, while decrease in the expression of matrix metalloproteinase-2 and 9. The aim of this study is to achieve standard formulation of GEGPA through stability, viscosity, distribution area, and drugs release for oral gel wound healing. Materials and Methods This is an in vitro and in vivo study with the randomized posttest only control group design. The gel was formulated according to the composition of each group by adding hydroxypropyl methylcellulose (HPMC), Lexgard, propylene glycol, and cold water to obtain 100 g of gel. Observations were made through the following tests: stability, viscosity, distribution area, drug release, and histopathological analysis of tooth extraction wound healing. Statistical analysis Data were analyzed using a one-way analysis of variance (α =...
Herbal medicine is interesting including treatments that use propolis. Propolis collected by Apis... more Herbal medicine is interesting including treatments that use propolis. Propolis collected by Apis mellifera bees has a very useful composition whose active ingredients are flavonoids, CAPE and saponin. This research aims to analyze the effect of Apis mellifera propolis on RUNX-2 and ALP during the remodeling period caused by the orthodontic tooth movement in Cavia cobaya. It constitutes an experimental laboratory study and a random sampling method. The method used experimental laboratory research on 28 Cavia cobaya, which were divided into four groups consisting of two control groups and two treatment groups which have been given 3% and 5% propolis extract respectively. RUNX-2 and ALP expression were evaluated using immunohistochemical staining. Materials and Methods: Propolis is a resin substance produced by bees. Cavia cobaya with orthodontic tooth movement by separator rubber. The control group (Group 1) included clinically healthy Cavia cobaya (n=7), (Group 2) included Cavia cob...
Mainly in developing countries, herbal medicine has remained the mainstay of approximately 75 to ... more Mainly in developing countries, herbal medicine has remained the mainstay of approximately 75 to 80 % of the world population. Mangosteen (Garcinia mangostana L.) fruit consists of several active components with major bioactive secondary metabolites that are xanthone derivatives. Traditionally, mangosteen has been used as a remedy for anti-diarrheal, skin infection on account of anti-bacterial, antioxidant, anti-inflammatory benefits which all lead to improving the general health. Nowadays, several studies discuss the benefits of mangosteen, on account of the fact that plenty of recent articles concerning the medical and oral benefits of mangosteen extracts abound. However, still no previous review (up to 2015) covered the oral benefits of the mangosteen. This review is new in that it addresses the recent updates of the oral benefits of mangosteen extracts. To achieve the study goal, this review covered recent published articles from 2015 onward, which focused only on the oral benef...
Background: Periodontitis is an inflammatory disease of the periodontal tissues usually provoked ... more Background: Periodontitis is an inflammatory disease of the periodontal tissues usually provoked by Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) bacteria. In the periodontitis healing process, macrophages play a significant role in the inflammatory stage. Brotowali (Tinospora crispa) consists of flavonoids that can stimulate periodontitis healing. Methods: Brotowali extract was derived from dried brotowali stems, processed into powder, and macerated with ethanol 80%. After that, the extraction was performed in three different concentrations, which were 25%, 50%, and 100%. five groups of Wistar rats were divided into Healthy wistar rats (control negative) Wistar rats suffering from periodontitis (control positive), Wistar rats suffering from periodontitis treated with 0.1 ml of 25% brotowali extract (treatment I), suffering from periodontitis treated with 0.1 ml of 50% brotowali extract (treatment II), and Wistar rats suffering from periodontitis treated with 0.1 m...
Journal of Taibah University Medical Sciences, 2021
Objective The treatment of periodontitis with scaling and root planing has a good prognosis. Howe... more Objective The treatment of periodontitis with scaling and root planing has a good prognosis. However, periodontitis may also exacerbate. The mucoadhesive patch is one of the distribution systems of topical drugs, which is not irritable to the mucosa and eventually increases permeability. Based on phytochemical screening, mangosteen peel extract has an active ingredient with high anti-inflammatory and antibacterial properties. This study aims to understand the potential of a mucoadhesive patch loaded with mangosteen peel extract to inhibit alveolar bone damage in periodontitis. Methods This experimental laboratory research was conducted using 27 Wistar rats divided into three groups: the positive control group (0.7% tetracycline gel application), negative control group (mucoadhesive patch application), and experimental group (mucoadhesive patch loaded with mangosteen peel extract application). Rats were administered 1 × 109 colony-forming unit as much as 200 μL of Actinobacillus actinomycetemcomitans bacteria three times at 2-day intervals. Tetracycline gel and mucoadhesive patch application were administered for 1 h/day for 3 days. Three rats from each group were sacrificed on days 3, 5, and 7 after the application. The lower jaw was dissected for histopathological examination using haematoxylin and eosin staining to determine the number of osteoclasts and osteoblasts. Results The mucoadhesive patches loaded with mangosteen peel extract significantly reduced the number of osteoclasts and increased the number of osteoblasts in all groups (p < 0.05). Conclusion The mucoadhesive patches loaded with mangosteen peel extract can prevent alveolar bone damage in periodontitis by inhibiting the number of osteoclasts and increasing the number of osteoblasts.
Background and Aim: The use of drugs as a therapy for traumatic ulcers may lead to drug resistanc... more Background and Aim: The use of drugs as a therapy for traumatic ulcers may lead to drug resistance and other side effects. Lactobacillus casei Shirota can affect the number of fibroblasts and blood vessels in wound healing. The aim of this study was to investigate the difference in the number of fibroblast cells and blood vessels after the topical and systemic administration of L. casei Shirota probiotics in Wistar rats with traumatic ulcer.
Materials and Methods: Overall, 36 healthy male Wistar rats aged 2-3 months old and weighing 175-250 g in body weight were used as a sample. Traumatic ulcer was made on the labial fornix incisive inferior. The subject rats were divided into groups: (1) A control group over 3 days, (2) a group that used distilled water over 7 days, (3) a group that underwent topical treatment over 3 days, (4) a group that used probiotics administered topically over 7 days, (5) a group that underwent systemic treatment over 3 days, and (6) a group that took oral probiotics for the traumatic ulcers over 7 days. The number of fibroblasts and blood vessels was observed through a hematoxylin-eosin examination.
Results: Based on the results of the study, a significant difference was observed in the number of fibroblasts (p=0.00) and blood vessels (p=0.018) in the 3-day topical group that underwent a 3-day systemic administration of probiotics compared with the number of fibroblast cells in the 7-day topical group and 7-day systemic group (p=0.00).
Conclusion: Overall, significant differences were observed in the number of fibroblasts and blood vessels in Wistar rats with traumatic ulcer after undergoing the topical and systemic administration of L. casei Shirota probiotics.
Background and Aim: The use of drugs as a therapy for traumatic ulcers may lead to drug resistanc... more Background and Aim: The use of drugs as a therapy for traumatic ulcers may lead to drug resistance and other side effects. Lactobacillus casei Shirota can affect the number of fibroblasts and blood vessels in wound healing. The aim of this study was to investigate the difference in the number of fibroblast cells and blood vessels after the topical and systemic administration of L. casei Shirota probiotics in Wistar rats with traumatic ulcer. Materials and Methods: Overall, 36 healthy male Wistar rats aged 2-3 months old and weighing 175-250 g in body weight were used as a sample. Traumatic ulcer was made on the labial fornix incisive inferior. The subject rats were divided into groups: (1) A control group over 3 days, (2) a group that used distilled water over 7 days, (3) a group that underwent topical treatment over 3 days, (4) a group that used probiotics administered topically over 7 days, (5) a group that underwent systemic treatment over 3 days, and (6) a group that took oral p...
Objective Stem cells of human exfoliated deciduous teeth (SHED) metabolites are secreted molecule... more Objective Stem cells of human exfoliated deciduous teeth (SHED) metabolites are secreted molecules from SHED, namely cytokines, chemokines, and growth factors. The metabolite can be used in various regenerative therapy based on cell-free immunomodulatory potential effects, like interleukin 10 (IL-10) and LL37. This molecule can stimulate with epigallocatechin gallate (EGCG) and α-mangosteen and has been proven to have anti-inflammatory and antibacterial effects. This study aimed to identify the effect of EGCG and α-mangosteen to SHED metabolite, called SHED-IL10 and SHED-LL37, from six passages to obtain the optimum stimulation and able to use as periodontitis regeneration treatment. Materials and Methods The six different passages of SHED were prepared in Dulbecco's modified Eagle medium and added with EGCG 80% (10 μM), EGCG 95% (10 μM), or α-mangosteen (10 μM). After a 24 hours incubation, each passage was measured with the metabolite concentration, SHED-IL10 and SHED-LL37, wi...
Background: Periodontitis is a progressive destructive periodontal disease. The prevalence of per... more Background: Periodontitis is a progressive destructive periodontal disease. The prevalence of periodontal disease in Indonesia reaches 74.1% and mostly occurs in the productive age group. Most of the periodontopathogenic bacteria are gram-negative bacteria and have endotoxin in the form of lipopolysaccharide (LPS), which can penetrate the periodontal tissue and induce an inflammatory response. In inflammatory conditions, osteoclastic activity is higher than osteoblastic activity, which causes bone destruction. This results in an imbalance between osteoclast-induced bone resorption and osteoblast-induced bone formation. The current preferred treatment for periodontitis is scaling root planning (SRP), but this therapy cannot repair the damaged periodontal tissue caused by periodontitis. Purpose: To describe the possibility of using a mucoadhesive gingival patch with Epigallocatechin-3-gallate (EGCG) green tea (Camellia sinensis) as alternative adjunct therapy for periodontal disease. ...
Background: Cancer is a major cause of death worldwide. One of the most common forms of cancer is... more Background: Cancer is a major cause of death worldwide. One of the most common forms of cancer is oral cancer, which can occur due to exposure to carcinogenic factors, such as tobacco cigarettes, alcohol, betel-nut chewing, ultraviolet rays or human papillomavirus infection. Physical exercise is known to have many benefits and can contribute to reducing the risk of cancer, minimising the side-effects of treatment and increasing the curative effect of cancer treatment. Purpose: This study aimed to explain the role of exercise as a method to reduce oral cancer risk. Reviews: Studies examining the impact of exercise on reducing oral cancer risk are currently limited due to a lack of research on this subject. However, according to several laboratory experimental research studies on Mus musculus test subjects, moderate-intensity exercise contributes to suppressing the proliferation and development of oral squamous epithelial cells, which can subsequently become cancer cells. Exercise can...
Objective Topical application of ambonese banana (Musa paradisiaca var. sapientum (L.) kuntze) st... more Objective Topical application of ambonese banana (Musa paradisiaca var. sapientum (L.) kuntze) stem sap gel (GEGPA) on the socket wound area showed an increase in the expression of platelet-derived growth factor-BB, while decrease in the expression of matrix metalloproteinase-2 and 9. The aim of this study is to achieve standard formulation of GEGPA through stability, viscosity, distribution area, and drugs release for oral gel wound healing. Materials and Methods This is an in vitro and in vivo study with the randomized posttest only control group design. The gel was formulated according to the composition of each group by adding hydroxypropyl methylcellulose (HPMC), Lexgard, propylene glycol, and cold water to obtain 100 g of gel. Observations were made through the following tests: stability, viscosity, distribution area, drug release, and histopathological analysis of tooth extraction wound healing. Statistical analysis Data were analyzed using a one-way analysis of variance (α =...
Herbal medicine is interesting including treatments that use propolis. Propolis collected by Apis... more Herbal medicine is interesting including treatments that use propolis. Propolis collected by Apis mellifera bees has a very useful composition whose active ingredients are flavonoids, CAPE and saponin. This research aims to analyze the effect of Apis mellifera propolis on RUNX-2 and ALP during the remodeling period caused by the orthodontic tooth movement in Cavia cobaya. It constitutes an experimental laboratory study and a random sampling method. The method used experimental laboratory research on 28 Cavia cobaya, which were divided into four groups consisting of two control groups and two treatment groups which have been given 3% and 5% propolis extract respectively. RUNX-2 and ALP expression were evaluated using immunohistochemical staining. Materials and Methods: Propolis is a resin substance produced by bees. Cavia cobaya with orthodontic tooth movement by separator rubber. The control group (Group 1) included clinically healthy Cavia cobaya (n=7), (Group 2) included Cavia cob...
Mainly in developing countries, herbal medicine has remained the mainstay of approximately 75 to ... more Mainly in developing countries, herbal medicine has remained the mainstay of approximately 75 to 80 % of the world population. Mangosteen (Garcinia mangostana L.) fruit consists of several active components with major bioactive secondary metabolites that are xanthone derivatives. Traditionally, mangosteen has been used as a remedy for anti-diarrheal, skin infection on account of anti-bacterial, antioxidant, anti-inflammatory benefits which all lead to improving the general health. Nowadays, several studies discuss the benefits of mangosteen, on account of the fact that plenty of recent articles concerning the medical and oral benefits of mangosteen extracts abound. However, still no previous review (up to 2015) covered the oral benefits of the mangosteen. This review is new in that it addresses the recent updates of the oral benefits of mangosteen extracts. To achieve the study goal, this review covered recent published articles from 2015 onward, which focused only on the oral benef...
Background: Periodontitis is an inflammatory disease of the periodontal tissues usually provoked ... more Background: Periodontitis is an inflammatory disease of the periodontal tissues usually provoked by Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) bacteria. In the periodontitis healing process, macrophages play a significant role in the inflammatory stage. Brotowali (Tinospora crispa) consists of flavonoids that can stimulate periodontitis healing. Methods: Brotowali extract was derived from dried brotowali stems, processed into powder, and macerated with ethanol 80%. After that, the extraction was performed in three different concentrations, which were 25%, 50%, and 100%. five groups of Wistar rats were divided into Healthy wistar rats (control negative) Wistar rats suffering from periodontitis (control positive), Wistar rats suffering from periodontitis treated with 0.1 ml of 25% brotowali extract (treatment I), suffering from periodontitis treated with 0.1 ml of 50% brotowali extract (treatment II), and Wistar rats suffering from periodontitis treated with 0.1 m...
Journal of Taibah University Medical Sciences, 2021
Objective The treatment of periodontitis with scaling and root planing has a good prognosis. Howe... more Objective The treatment of periodontitis with scaling and root planing has a good prognosis. However, periodontitis may also exacerbate. The mucoadhesive patch is one of the distribution systems of topical drugs, which is not irritable to the mucosa and eventually increases permeability. Based on phytochemical screening, mangosteen peel extract has an active ingredient with high anti-inflammatory and antibacterial properties. This study aims to understand the potential of a mucoadhesive patch loaded with mangosteen peel extract to inhibit alveolar bone damage in periodontitis. Methods This experimental laboratory research was conducted using 27 Wistar rats divided into three groups: the positive control group (0.7% tetracycline gel application), negative control group (mucoadhesive patch application), and experimental group (mucoadhesive patch loaded with mangosteen peel extract application). Rats were administered 1 × 109 colony-forming unit as much as 200 μL of Actinobacillus actinomycetemcomitans bacteria three times at 2-day intervals. Tetracycline gel and mucoadhesive patch application were administered for 1 h/day for 3 days. Three rats from each group were sacrificed on days 3, 5, and 7 after the application. The lower jaw was dissected for histopathological examination using haematoxylin and eosin staining to determine the number of osteoclasts and osteoblasts. Results The mucoadhesive patches loaded with mangosteen peel extract significantly reduced the number of osteoclasts and increased the number of osteoblasts in all groups (p < 0.05). Conclusion The mucoadhesive patches loaded with mangosteen peel extract can prevent alveolar bone damage in periodontitis by inhibiting the number of osteoclasts and increasing the number of osteoblasts.
Background and Aim: The use of drugs as a therapy for traumatic ulcers may lead to drug resistanc... more Background and Aim: The use of drugs as a therapy for traumatic ulcers may lead to drug resistance and other side effects. Lactobacillus casei Shirota can affect the number of fibroblasts and blood vessels in wound healing. The aim of this study was to investigate the difference in the number of fibroblast cells and blood vessels after the topical and systemic administration of L. casei Shirota probiotics in Wistar rats with traumatic ulcer.
Materials and Methods: Overall, 36 healthy male Wistar rats aged 2-3 months old and weighing 175-250 g in body weight were used as a sample. Traumatic ulcer was made on the labial fornix incisive inferior. The subject rats were divided into groups: (1) A control group over 3 days, (2) a group that used distilled water over 7 days, (3) a group that underwent topical treatment over 3 days, (4) a group that used probiotics administered topically over 7 days, (5) a group that underwent systemic treatment over 3 days, and (6) a group that took oral probiotics for the traumatic ulcers over 7 days. The number of fibroblasts and blood vessels was observed through a hematoxylin-eosin examination.
Results: Based on the results of the study, a significant difference was observed in the number of fibroblasts (p=0.00) and blood vessels (p=0.018) in the 3-day topical group that underwent a 3-day systemic administration of probiotics compared with the number of fibroblast cells in the 7-day topical group and 7-day systemic group (p=0.00).
Conclusion: Overall, significant differences were observed in the number of fibroblasts and blood vessels in Wistar rats with traumatic ulcer after undergoing the topical and systemic administration of L. casei Shirota probiotics.
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Papers by Mohammed Ahmed Aljunaid
Materials and Methods: Overall, 36 healthy male Wistar rats aged 2-3 months old and weighing 175-250 g in body weight were used as a sample. Traumatic ulcer was made on the labial fornix incisive inferior. The subject rats were divided into groups: (1) A control group over 3 days, (2) a group that used distilled water over 7 days, (3) a group that underwent topical treatment over 3 days, (4) a group that used probiotics administered topically over 7 days, (5) a group that underwent systemic treatment over 3 days, and (6) a group that took oral probiotics for the traumatic ulcers over 7 days. The number of fibroblasts and blood vessels was observed through a hematoxylin-eosin examination.
Results: Based on the results of the study, a significant difference was observed in the number of fibroblasts (p=0.00) and blood vessels (p=0.018) in the 3-day topical group that underwent a 3-day systemic administration of probiotics compared with the number of fibroblast cells in the 7-day topical group and 7-day systemic group (p=0.00).
Conclusion: Overall, significant differences were observed in the number of fibroblasts and blood vessels in Wistar rats with traumatic ulcer after undergoing the topical and systemic administration of L. casei Shirota probiotics.
Materials and Methods: Overall, 36 healthy male Wistar rats aged 2-3 months old and weighing 175-250 g in body weight were used as a sample. Traumatic ulcer was made on the labial fornix incisive inferior. The subject rats were divided into groups: (1) A control group over 3 days, (2) a group that used distilled water over 7 days, (3) a group that underwent topical treatment over 3 days, (4) a group that used probiotics administered topically over 7 days, (5) a group that underwent systemic treatment over 3 days, and (6) a group that took oral probiotics for the traumatic ulcers over 7 days. The number of fibroblasts and blood vessels was observed through a hematoxylin-eosin examination.
Results: Based on the results of the study, a significant difference was observed in the number of fibroblasts (p=0.00) and blood vessels (p=0.018) in the 3-day topical group that underwent a 3-day systemic administration of probiotics compared with the number of fibroblast cells in the 7-day topical group and 7-day systemic group (p=0.00).
Conclusion: Overall, significant differences were observed in the number of fibroblasts and blood vessels in Wistar rats with traumatic ulcer after undergoing the topical and systemic administration of L. casei Shirota probiotics.