Dental Medicine

The aim of this study was to analyze the effect of moderate exercise on the elevation of Bax/Bcl-2 ratio. Eighteen strain Swiss Webster (Balb/c) were divided into three groups (n=6). K1 and K2 had contact with water 3 times/week for 12 weeks, while the members of the K3 group swam 3 times/week for 12 weeks while carrying load weighed 3% of their body weight. After 5 weeks, they were induced with 0.04 ml oleum olivarum (K1), 0.08 mg benzopyrene/0.04 ml oleum olivarum (K2, K3) 3 times/week for 4 weeks. Immunohistochemistry assays were used to determine the ratio of Bax/Bcl-2 expression. The results were analyzed using an independent t-test. The Bax/Bcl-2 ratio increased significantly in K3 compared to K2 (p=0.00). Moderate exercise could increase the Bax/Bcl-2 ratio in oral squamous epithelial cells induced by benzopyrene.

How to cite this article: Irmawati A, Jasmin N, Sidarningsih (2018) The effect of moderate exercise on the elevation of Bax/Bcl-2 ratio in oral squamous epithelial cells induced by benzopyrene, Veterinary World, 11(2): 177-180. Abstract Aim: The aim of this study was to analyze the effect of moderate exercise on the elevation of Bax/Bcl-2 ratio. Materials and Methods: Eighteen Mus musculus strain Swiss Webster (Balb/c) were divided into three groups (n=6). K1 and K2 had contact with water 3 times/week for 12 weeks, while the members of the K3 group swam 3 times/week for 12 weeks while carrying load weighed 3% of their body weight. After 5 weeks, they were induced with 0.04 ml oleum olivarum (K1), 0.08 mg benzopyrene/0.04 ml oleum olivarum (K2, K3) 3 times/week for 4 weeks. Immunohistochemistry assays were used to determine the ratio of Bax/Bcl-2 expression. The results were analyzed using an independent t-test. Result: The Bax/Bcl-2 ratio increased significantly in K3 compared to K2...

Pendahuluan. Akumulasi plak pada gigi tiruan mengakibatkan beberapa perubahan patologis dalam rongga mulut. Komponen pada plak terdiri atas 20%- 30% matriks ekstraselluler dan sekitar 70% mikroorganisme. Alternatif bahan pembersih plak pada gigi tiruan berbahan herbal dibutuhkan untuk menghidrolisis plak pada gigi tiruan. Ekstrak Jintan Hitam (Nigella sativa) mempunyai 3 zat aktif yang dapat menghidrolisis plak pada gigi tiruan. Tujuan. Tujuan dari penelitian ini adalah untuk menganalisa konsentrasi yang efekif dari ekstrak Jintan Hitam dan lama waktu perendaman yang efektif untuk menghidrolisis plak pada gigi tiruan. Metode. Penelitian yang dilakukan adalah penelitian eksperimental laboratoris. Setelah pemakaian gigi tiruan selama 24 jam, plak pada gigi tiruan diekskavasi dan direplikasi pada BHIB. Plat akrilik direndam dalam BHIB kemudian direndam kedalam larutan kontrol (Aquades) dan ekstrak Jintam Hitam dalam berbagai variasi konsentrasi dan lama waktu perendaman. Larutan hasil ...

Background: Oral Hygiene status is expressed in periodontal diseases prevalence, which generally caused by poor oral hygiene, i.e plaque accumulation. Some studies have shown that prevalence of periodontal disease is still high in Indonesia. Primary Heallth Center is the first health care that achivable by community, so that, primary health care is expected can decrease dental problem in community. Purpose:The aim of this study is to get information about oral hygiene status of patients in Pucang Sewu Primary Health Center. Method: This research is an descriptive observasional. There are 100 patients that are studied. Simple random sampling is used in this research. This research use OHI-S Index and questionnaire. The data are showed in table and percentage and analyzed using cross tabulation. Results: The result of this study showed that 89% subjects had good oral hygiene, 11% had fair oral hygiene and no one had poor oral hygiene. Conclusion: Most of patients in Pucang Sewu Primar...

Background: Acrylic based denture always contact with the mucosa (both palatal and gingival). Denture needs to be cleaned by immersed of liquid chemical. In this case, the chemical liquid is durian rind extract. This extract cointains flavonoid, saponin, tanin that have antimicrobial effect which can against Candida albicans. Purpose: To know the ability of durian rind extract in inhibiting the growth of Candida albicans’ colony on acrylic based denture. Material and Method: 27 heat cured acrylic plates in “dough stage” is embedded into the mold and the acrylic is then cured. Then it was was being sterilized. These acrylic were immersed in saliva and then in suspension of Candida albicans. Afterward they were incubated at 37C for 24 hours. They were divided into 4 groups. First group immersed in sterile aquadest, the second in 20% durian rind extract, the third is immersed in 25% durian rind extract, and the last is in 30% durian rind extract. All groups are immersed for 30 minutes....

Latar Belakang. Pemeriksaan radiografi mempunyai peranan yang sangat penting di bidang kedokteran gigi yaitu untuk menegakkan diagnosis dan rencana perawatan. Salah satu Radiografi yang sering digunakan di bidang kedokteran gigi adalah radiografi panoramik Gambaran radiografi panoramik dapat memperlihatkan struktur maksila dan mandibula dalam satu gambaran radiografi, termasuk struktur di sekitarnya seperti fossa nasalis, prosesus styloideus, dan sendi temporomandibula. Foto panoramik dapat juga melihat manifestasi penyakit-penyakit sistemik di rongga mulut, salah satunya adalah osteoporosis. Osteopenia merupakan prediktor awal terjadinya osteoporosis, dimana terjadi penurunan BMD. Rendahnya BMD pada wanita post-menopause dapat mempengaruhi morfologi kondilus sendi temporomandibula. Salah satunya adalah dapat menyebabkan erosi. Tujuan. Mengetahui erosi kondilus sendi temporomandibula pada wanita post-menopause dengan osteopenia melalui pengamatan radiografi panoramik. Metode. Peneli...

Background: More than 200 million people worldwide diagnosed with osteoporosis, a degenerative condition characterized by decreasing bone mineral density. Although oral bisphosphonates are the most used form of treatment for osteoporosis, they have the potential to result in osteonecrosis. Osthole bone marrow mesenchymal stem cells (OBMMSCs) are combination of bone marrow mesenchymal stem cells (BMMSCs) and coumarin derivatives from Cnidium monnieri act as anti-inflammatory and anti-osteoporosis agents. The use of OBMMSCs in triad tissue engineering necessitates using a growth factor and a scaffold, which are combined with BMP-9 incorporated in carbon-based scaffold namely nano hydroxyapatite/collagen I/multi-walled carbon nanotubes (nHA/ColI/MWCNTs) scaffold to promote better loading. The combination of OBMMSCs and BMP-9 loaded nHA/ColI/MWCNTs scaffold has potential to increase OBMMSC differentiation into osteoblasts, resulting in increased bone remodeling and a better outcome in o...

kedokteran gigi yang melibatkan jaringan keras dan jaringan lunak pada rongga mulut. Tindakan pencabutan gigi akan menimbulkan luka pada soket yang kemudian secara fisiologis akan mengalami proses penyembuhan apabila tidak mengalami hambatan seperti infeksi atau penanganan luka yang tidak adekuat. Proses penyembuhan luka melibatkan makrofag yang berperan pada proses keradangan dan penyembuhan luka. Terdapat berbagai bahan alternatif yang dapat digunakan untuk membantu proses penyembuhan luka, salah satunya adalah sereh. Daun sereh (Cymbopogon citratus) merupakan salah satu bahan alternatif yang memiliki kandungan citral dan flavonoid yang berperan dalam proses penyembuhan luka dan penurunan keradangan. Tujuan: Untuk mengetahui apakah pemberian ekstrak daun sereh berpengaruh pada penurunan jumlah makrofag pada soket pasca pencabutan gigi tikus wistar.Metode: 30 ekor tikus wistar dibagi menjadi 6 kelompok, yaitu 3 kelompok kontrol dan 3 kelompok perlakuan. Kelompok kontrol dilakukan p...

Objective The treatment of periodontitis with scaling and root planing has a good prognosis. However, periodontitis may also exacerbate. The mucoadhesive patch is one of the distribution systems of topical drugs, which is not irritable to the mucosa and eventually increases permeability. Based on phytochemical screening, mangosteen peel extract has an active ingredient with high anti-inflammatory and antibacterial properties. This study aims to understand the potential of a mucoadhesive patch loaded with mangosteen peel extract to inhibit alveolar bone damage in periodontitis. Methods This experimental laboratory research was conducted using 27 Wistar rats divided into three groups: the positive control group (0.7% tetracycline gel application), negative control group (mucoadhesive patch application), and experimental group (mucoadhesive patch loaded with mangosteen peel extract application). Rats were administered 1 × 109 colony-forming unit as much as 200 μL of Actinobacillus actinomycetemcomitans bacteria three times at 2-day intervals. Tetracycline gel and mucoadhesive patch application were administered for 1 h/day for 3 days. Three rats from each group were sacrificed on days 3, 5, and 7 after the application. The lower jaw was dissected for histopathological examination using haematoxylin and eosin staining to determine the number of osteoclasts and osteoblasts. Results The mucoadhesive patches loaded with mangosteen peel extract significantly reduced the number of osteoclasts and increased the number of osteoblasts in all groups (p < 0.05). Conclusion The mucoadhesive patches loaded with mangosteen peel extract can prevent alveolar bone damage in periodontitis by inhibiting the number of osteoclasts and increasing the number of osteoblasts.

Background: Periodontitis is an inflammatory disease of the periodontal tissues usually provoked by Actinobacillus actinomycetemcomitans (A. actinomycetemcomitans) bacteria. In the periodontitis healing process, macrophages play a significant role in the inflammatory stage. Brotowali (Tinospora crispa) consists of flavonoids that can stimulate periodontitis healing. Methods: Brotowali extract was derived from dried brotowali stems, processed into powder, and macerated with ethanol 80%. After that, the extraction was performed in three different concentrations, which were 25%, 50%, and 100%. five groups of Wistar rats were divided into Healthy wistar rats (control negative) Wistar rats suffering from periodontitis (control positive), Wistar rats suffering from periodontitis treated with 0.1 ml of 25% brotowali extract (treatment I), suffering from periodontitis treated with 0.1 ml of 50% brotowali extract (treatment II), and Wistar rats suffering from periodontitis treated with 0.1 m...

Mainly in developing countries, herbal medicine has remained the mainstay of approximately 75 to 80 % of the world population. Mangosteen (Garcinia mangostana L.) fruit consists of several active components with major bioactive secondary metabolites that are xanthone derivatives. Traditionally, mangosteen has been used as a remedy for anti-diarrheal, skin infection on account of anti-bacterial, antioxidant, anti-inflammatory benefits which all lead to improving the general health. Nowadays, several studies discuss the benefits of mangosteen, on account of the fact that plenty of recent articles concerning the medical and oral benefits of mangosteen extracts abound. However, still no previous review (up to 2015) covered the oral benefits of the mangosteen. This review is new in that it addresses the recent updates of the oral benefits of mangosteen extracts. To achieve the study goal, this review covered recent published articles from 2015 onward, which focused only on the oral benef...

Herbal medicine is interesting including treatments that use propolis. Propolis collected by Apis mellifera bees has a very useful composition whose active ingredients are flavonoids, CAPE and saponin. This research aims to analyze the effect of Apis mellifera propolis on RUNX-2 and ALP during the remodeling period caused by the orthodontic tooth movement in Cavia cobaya. It constitutes an experimental laboratory study and a random sampling method. The method used experimental laboratory research on 28 Cavia cobaya, which were divided into four groups consisting of two control groups and two treatment groups which have been given 3% and 5% propolis extract respectively. RUNX-2 and ALP expression were evaluated using immunohistochemical staining. Materials and Methods: Propolis is a resin substance produced by bees. Cavia cobaya with orthodontic tooth movement by separator rubber. The control group (Group 1) included clinically healthy Cavia cobaya (n=7), (Group 2) included Cavia cob...

Objective Topical application of ambonese banana (Musa paradisiaca var. sapientum (L.) kuntze) stem sap gel (GEGPA) on the socket wound area showed an increase in the expression of platelet-derived growth factor-BB, while decrease in the expression of matrix metalloproteinase-2 and 9. The aim of this study is to achieve standard formulation of GEGPA through stability, viscosity, distribution area, and drugs release for oral gel wound healing. Materials and Methods This is an in vitro and in vivo study with the randomized posttest only control group design. The gel was formulated according to the composition of each group by adding hydroxypropyl methylcellulose (HPMC), Lexgard, propylene glycol, and cold water to obtain 100 g of gel. Observations were made through the following tests: stability, viscosity, distribution area, drug release, and histopathological analysis of tooth extraction wound healing. Statistical analysis Data were analyzed using a one-way analysis of variance (α =...

Background: Cancer is a major cause of death worldwide. One of the most common forms of cancer is oral cancer, which can occur due to exposure to carcinogenic factors, such as tobacco cigarettes, alcohol, betel-nut chewing, ultraviolet rays or human papillomavirus infection. Physical exercise is known to have many benefits and can contribute to reducing the risk of cancer, minimising the side-effects of treatment and increasing the curative effect of cancer treatment. Purpose: This study aimed to explain the role of exercise as a method to reduce oral cancer risk. Reviews: Studies examining the impact of exercise on reducing oral cancer risk are currently limited due to a lack of research on this subject. However, according to several laboratory experimental research studies on Mus musculus test subjects, moderate-intensity exercise contributes to suppressing the proliferation and development of oral squamous epithelial cells, which can subsequently become cancer cells. Exercise can...