Sesame has great potential as an industrial crop but its production is challenged by drought and ... more Sesame has great potential as an industrial crop but its production is challenged by drought and salt stresses. To unravel the genetic variants leading to salinity and drought tolerances at the germination stage, genome-wide association studies of stress tolerance indexes related to NaCl-salt and polyethylene glycol-drought induced stresses were performed with a diversity panel of 490 sesame accessions. An extensive variation was observed for drought and salt responses in the population and most of the accessions were moderately tolerant to both stresses. A total of 132 and 120 significant Single Nucleotide Polymorphisms (SNPs) resolved to nine and 15 Quantitative trait loci (QTLs) were detected for drought and salt stresses, respectively. Only two common QTLs for drought and salt responses were found located on linkage groups 5 and 7, respectively. This indicates that the genetic bases for drought and salt responses in sesame are different. A total of 13 and 27 potential candidate genes were uncovered for drought and salt tolerance indexes, respectively, encoding transcription factors, antioxidative enzymes, osmoprotectants and involved in hormonal biosynthesis, signal transduction or ion sequestration. The identified SNPs and potential candidate genes represent valuable resources for future functional characterization towards the enhancement of sesame cultivars for drought and salt tolerances.
The MYB gene family constitutes one of the largest transcription factors (TFs) modulating various... more The MYB gene family constitutes one of the largest transcription factors (TFs) modulating various biological processes in plants. Although genome-wide analysis of this gene family has been carried out in some species, only three MYB members have been functionally characterized heretofore in sesame (Sesamum indicum L.). Here, we identified a relatively high number (287) of sesame MYB genes (SIMYBs) with an uncommon overrepresentation of the 1R-subfamily. A total of 95% of SIMYBs was mapped unevenly onto the 16 linkage groups of the sesame genome with 55 SIMYBs tandemly duplicated. In addition, molecular characterization, gene structure, and evolutionary relationships of SIMYBs were established. Based on the close relationship between sesame and Arabidopsis thaliana, we uncovered that the functions of SIMYBs are highly diverse. A total of 65% of SIMYBs were commonly detected in five tissues, suggesting that they represent key TFs modulating sesame growth and development. Moreover, we found that SIMYBs regulate sesame responses to drought and waterlogging, which highlights the potential of SIMYBs towards improving stress tolerance in sesame. This work presents a comprehensive picture of the MYB gene family in sesame and paves the way for further functional validation of the members of this versatile gene family.
Near-infrared reflectance spectroscopy reveals wide variation in major components of sesame seeds from Africa and Asia, 2018
Sesame is an important oilseed crop in Africa and Asia, owing to its high nutritional quality
se... more Sesame is an important oilseed crop in Africa and Asia, owing to its high nutritional quality
seed and market value. Variation in sesame seed components including oil, oleic acid,
linoleic acid, and protein was investigated by near-infrared reflectance spectrometry in 139
samples collected from different countries. Oil and protein contents were between 40.8%
and 60.3% (mean 53.0%) and 15.5% to 25.5% (mean 20.4%), respectively. Linoleic acid,
ranging from 31.8% to 52.4% (mean 46%) was more abundant than oleic acid (31.8%–50.6%,
mean 38.1%). Light-seeded samples displayed higher nutritional quality, as they were richer
in oil, protein, and linoleic acid than dark seeds. Samples from Africa had higher oil and
linoleic acid contents, while Asian samples had higher oleic content. The analysis revealed
West African sesame cultivars containing especially high levels of seed components, which
may command high market values. Two clusters of sesame samples grouped by seed
composition were obtained, including one cluster with high oil and oleic acid content and
the other with high protein and linoleic acid content. This study revealed that sesame
samples from Africa and Asia harbor high variation for major seed components and also
provided background information for breeding high-nutrition varieties according to the
demands of sesame seed markets.
Sesame is an important crop in West and Central Africa playing a role of an alternative cash crop... more Sesame is an important crop in West and Central Africa playing a role of an alternative cash crop for smallholders. However, sesame productivity is highly impaired by drought. This study aimed at identifying some drought-resistant genotypes and efficient screening traits in large sesame germplasm. Ten genotypes were examined based on 21 biochemical, physiological, agromorphological and seed quality traits under three weeks of water stress. A high variability for drought resistance was observed among the genotypes. The genotypes WC17, WC18 and WC14 were drought resistant, WC12, WC13, WC06 and WC03 were moderately drought resistant while, WC02, WC10 and WC08 were drought sensitive, based on principal component analysis. The resistant genotypes exhibited both avoidance and tolerance features including increase of the root system, reduced water loss, highest activity of antioxidative enzymes and accumulation of proline. They produced higher biomass and had higher ability to maintain seed quality under drought stress compared with the sensitive genotypes. Strong accumulation (~200% ratio stress/control) of biochemical markers including superoxide dismutase, ascorbate peroxidase, catalase and proline could be regarded as an important indicator for selecting drought resistant genotypes. This study represents a reference for future research towards developing new varieties with improved drought resistance in West and Central Africa.
Background: West Africa's Sahel is characterized by a dry and hot climate with limited rainfall t... more Background: West Africa's Sahel is characterized by a dry and hot climate with limited rainfall that impairs the production of several crops. Sesame is a resilient crop that is well suited to this environment. Unfortunately, there is a lack of data relative to the status of its production in West Africa. We made investigations in four major sesame-growing areas of Senegal and Mali, into the status of the crop's production, its agronomic practices, the challenges farmers face and their preferences concerning the traits that should be improved. Results: A total of 256 sesame producers in 47 villages were interviewed using a semi-structured questionnaire. The results showed that sesame is a multi-ethnic crop and only 20% of the total fields owned by farmers were allocated to its cultivation. The yield and the seasonal production of sesame per farmer was quite weak showing that this crop is still a commodity grown on a small scale. Various cultivars were grown, and the most widely grown ones have considerable levels of oil (53–60.34%) and protein (18–21.89%) contents. In both countries, seed marketing was the main impediment the producers faced on account of a lack of reliable markets and of a considerable fluctuation in prices. Conclusions: Overall, the sesame sector is still traditional but is progressively developing and sesame could become an important cash crop for smallholders in West Africa's Sahel. Research programs should target the release of the varieties with higher yield, a stronger resistance to drought, heat, diseases and pests, a good seed quality and improved plant architecture. This study represents the first insight into the sesame sector in West Africa's Sahel, and our findings may guide researchers and policy-makers to boost this sector for ensuring food security and the improvement of small-scale farmers' livelihood.
Drought is one of the most important abiotic stresses that impair sesame (Sesamum indicum L.) pro... more Drought is one of the most important abiotic stresses that impair sesame (Sesamum indicum L.) productivity mainly when it occurs at flowering stage. However up to now, very few studies have attempted to investigate the molecular responses of sesame to drought stress. In this experiment, two genotypes having contrasting responses to drought (tolerant and sensitive) were submitted to progressive drought followed by recovering stage at flowering stage. RNAs were isolated from roots of plants before drought stress, at 3-time points during progressive drought, after rewatering, and sequenced using Illumina HiSeq 4000 platform. These RNA-Seq resources (BioSample IDs: SAMN06130606 and SAMN06130607) provided an opportunity to elucidate the molecular responses of sesame to drought and find out some candidate genes for drought tolerance improvement.
Sesame is a survivor crop cultivated for ages in arid areas under high temperatures and limited w... more Sesame is a survivor crop cultivated for ages in arid areas under high temperatures and limited water conditions. Since its entire genome has been sequenced, revealing evolution, and functional characterization of its abiotic stress genes became a hot topic. In this study, we performed a whole-genome identification and analysis of Hsf gene family in sesame. Thirty genes encoding Hsf domain were found and classified into 3 major classes A, B, and C. The class A members were the most representative one and Hsf genes were distributed in 12 of the 16 linkage groups (except the LG 8, 9, 13, and 16). Evolutionary analysis revealed that, segmental duplication events which occurred around 67 MYA, were the primary force underlying Hsf genes expansion in sesame. Comparative analysis also suggested that sesame has retained most of its Hsf genes while its relatives viz. tomato and potato underwent extensive gene losses during evolution. Continuous purifying selection has played a key role in the maintenance of Hsf genes in sesame. Expression analysis of the Hsf genes in sesame revealed their putative involvement in multiple tissue-/developmental stages. Time-course expression profiling of Hsf genes in response to drought stress showed that 90% Hsfs are drought responsive. We infer that classes B-Hsfs might be the primary regulators of drought response in sesame by cooperating with some class A genes. This is the first insight into this gene family and the results provide some gene resources for future gene cloning and functional studies toward the improvement in stress tolerance of sesame.
Sesame is one of the oldest oilseed crops grown
mainly in Africa and Asia. Although genetic and g... more Sesame is one of the oldest oilseed crops grown mainly in Africa and Asia. Although genetic and genomic studies on sesame have started late, the past 5 years have witnessed extensive progresses in these areas on this crop. Important genomic sequence resources such as functional markers, genes and QTLs linked to agronomically important traits, have been generated through linkage mapping and association analysis to assist sesame improvement programs. However, most of these data are scattered in different maps making them hard to be exploited efficiently in breeding programs. In this study, we report a comprehensive physical map gathering 151 published genomic sequence resources which highlighted some hotspot functional regions in the sesame genome. Moreover, 83,135 non-redundant SSRs have been supplied along with their physical position and motif composition. This will assist future research in fine mapping or pinpointing more functional genes based on the already published QTLs and functional markers. This physical map represents a good landmark for further non-overlapping genetic and genomic studies working towards sesame improvement.
Microsatellite DNAs (or SSRs) are important genomic components involved in many important biologi... more Microsatellite DNAs (or SSRs) are important genomic components involved in many important biological functions. SSRs have been extensively exploited as molecular markers for diverse applications including genetic diversity, linkage/association mapping of gene/QTL, marker-assisted selection, variety identification and evolution analysis. However, a comprehensive database or web service for studying mi-crosatellite DNAs and marker development in plants is lacking. Here, we developed a database, PMD-Base, which integrates large amounts of microsatel-lite DNAs from genome sequenced plant species and includes a web service for microsatellite DNAs identification. In PMDBase, 26 230 099 microsatellite DNAs were identified spanning 110 plant species. Up to three pairs of primers were supplied for every microsatellite DNA. For 81 species, genomic features of the microsatellite DNAs (genic or non-genic) were supplied with the corresponding genes or transcripts from public databases. Microsatellite DNAs can be explored through browsing and searching modules with a user-friendly web interface and cus-tomized software. Furthermore, we developed MIS-Aweb and embedded Primer3web to help users to identify microsatellite DNAs and design corresponding primers in their own genomic sequences online. All datasets of microsatellite DNAs can be down-loaded conveniently. PMDBase will be updated regularly with new available genome data and can be accessed freely via the address http://www.sesame-bioinfo.org/PMDBase.
Sesame (Sesamum indicum L.) is one of the most important oilseed crops. It is mainly grown in ari... more Sesame (Sesamum indicum L.) is one of the most important oilseed crops. It is mainly grown in arid and semi-arid regions with occurrence of unpredictable drought which is one of the major constraints of its production. However, the lack of gene resources associated with drought tolerance hinders sesame genetic improvement towards this osmotic stress. The present research aimed at identifying candidate genes associated with drought tolerance in the whole genome of sesame through homology search of known drought associated genes from three relative species, viz., potato, tomato and the well-described model plant Arabidopsis. Based on 2,495 sequences including 1,150 from Arabidopsis, 1,075 from potato and 270 from tomato, comparative analysis against sesame genome led to the identification of a set of 75 candidate genes (42, 22 and 11 from Arabidopsis, potato and tomato, respectively). Mapping results showed that the candidate genes were distributed on the 16 sesame linkage groups. Wide range of genes with various functions identified in this study confirmed that drought tolerance in sesame is under the control of several genes. Based on their functional classification, 2 groups of candidate genes were identified: (a) genes which protect the plant against drought effect; (b) signal transduction genes and transcription factors. Many transcription factors were retrieved including 6 AP2/ERF genes among which 3 were more expressed in drought tolerant material compared to the sensitive one and might play some important roles in drought tolerance in sesame. Our results provided genomic resources for further functional analysis and genetic engineering towards drought tolerance improvement in sesame.
Background: Sesame is an important oilseed crop mainly grown in inclement areas with high tempera... more Background: Sesame is an important oilseed crop mainly grown in inclement areas with high temperatures and frequent drought. Thus, drought constitutes one of the major constraints of its production. The AP2/ERF is a large family of transcription factors known to play significant roles in various plant processes including biotic and abiotic stress responses. Despite their importance, little is known about sesame AP2/ERF genes. This constitutes a limitation for drought-tolerance candidate genes discovery and breeding for tolerance to water deficit.
Sesame has great potential as an industrial crop but its production is challenged by drought and ... more Sesame has great potential as an industrial crop but its production is challenged by drought and salt stresses. To unravel the genetic variants leading to salinity and drought tolerances at the germination stage, genome-wide association studies of stress tolerance indexes related to NaCl-salt and polyethylene glycol-drought induced stresses were performed with a diversity panel of 490 sesame accessions. An extensive variation was observed for drought and salt responses in the population and most of the accessions were moderately tolerant to both stresses. A total of 132 and 120 significant Single Nucleotide Polymorphisms (SNPs) resolved to nine and 15 Quantitative trait loci (QTLs) were detected for drought and salt stresses, respectively. Only two common QTLs for drought and salt responses were found located on linkage groups 5 and 7, respectively. This indicates that the genetic bases for drought and salt responses in sesame are different. A total of 13 and 27 potential candidate genes were uncovered for drought and salt tolerance indexes, respectively, encoding transcription factors, antioxidative enzymes, osmoprotectants and involved in hormonal biosynthesis, signal transduction or ion sequestration. The identified SNPs and potential candidate genes represent valuable resources for future functional characterization towards the enhancement of sesame cultivars for drought and salt tolerances.
The MYB gene family constitutes one of the largest transcription factors (TFs) modulating various... more The MYB gene family constitutes one of the largest transcription factors (TFs) modulating various biological processes in plants. Although genome-wide analysis of this gene family has been carried out in some species, only three MYB members have been functionally characterized heretofore in sesame (Sesamum indicum L.). Here, we identified a relatively high number (287) of sesame MYB genes (SIMYBs) with an uncommon overrepresentation of the 1R-subfamily. A total of 95% of SIMYBs was mapped unevenly onto the 16 linkage groups of the sesame genome with 55 SIMYBs tandemly duplicated. In addition, molecular characterization, gene structure, and evolutionary relationships of SIMYBs were established. Based on the close relationship between sesame and Arabidopsis thaliana, we uncovered that the functions of SIMYBs are highly diverse. A total of 65% of SIMYBs were commonly detected in five tissues, suggesting that they represent key TFs modulating sesame growth and development. Moreover, we found that SIMYBs regulate sesame responses to drought and waterlogging, which highlights the potential of SIMYBs towards improving stress tolerance in sesame. This work presents a comprehensive picture of the MYB gene family in sesame and paves the way for further functional validation of the members of this versatile gene family.
Near-infrared reflectance spectroscopy reveals wide variation in major components of sesame seeds from Africa and Asia, 2018
Sesame is an important oilseed crop in Africa and Asia, owing to its high nutritional quality
se... more Sesame is an important oilseed crop in Africa and Asia, owing to its high nutritional quality
seed and market value. Variation in sesame seed components including oil, oleic acid,
linoleic acid, and protein was investigated by near-infrared reflectance spectrometry in 139
samples collected from different countries. Oil and protein contents were between 40.8%
and 60.3% (mean 53.0%) and 15.5% to 25.5% (mean 20.4%), respectively. Linoleic acid,
ranging from 31.8% to 52.4% (mean 46%) was more abundant than oleic acid (31.8%–50.6%,
mean 38.1%). Light-seeded samples displayed higher nutritional quality, as they were richer
in oil, protein, and linoleic acid than dark seeds. Samples from Africa had higher oil and
linoleic acid contents, while Asian samples had higher oleic content. The analysis revealed
West African sesame cultivars containing especially high levels of seed components, which
may command high market values. Two clusters of sesame samples grouped by seed
composition were obtained, including one cluster with high oil and oleic acid content and
the other with high protein and linoleic acid content. This study revealed that sesame
samples from Africa and Asia harbor high variation for major seed components and also
provided background information for breeding high-nutrition varieties according to the
demands of sesame seed markets.
Sesame is an important crop in West and Central Africa playing a role of an alternative cash crop... more Sesame is an important crop in West and Central Africa playing a role of an alternative cash crop for smallholders. However, sesame productivity is highly impaired by drought. This study aimed at identifying some drought-resistant genotypes and efficient screening traits in large sesame germplasm. Ten genotypes were examined based on 21 biochemical, physiological, agromorphological and seed quality traits under three weeks of water stress. A high variability for drought resistance was observed among the genotypes. The genotypes WC17, WC18 and WC14 were drought resistant, WC12, WC13, WC06 and WC03 were moderately drought resistant while, WC02, WC10 and WC08 were drought sensitive, based on principal component analysis. The resistant genotypes exhibited both avoidance and tolerance features including increase of the root system, reduced water loss, highest activity of antioxidative enzymes and accumulation of proline. They produced higher biomass and had higher ability to maintain seed quality under drought stress compared with the sensitive genotypes. Strong accumulation (~200% ratio stress/control) of biochemical markers including superoxide dismutase, ascorbate peroxidase, catalase and proline could be regarded as an important indicator for selecting drought resistant genotypes. This study represents a reference for future research towards developing new varieties with improved drought resistance in West and Central Africa.
Background: West Africa's Sahel is characterized by a dry and hot climate with limited rainfall t... more Background: West Africa's Sahel is characterized by a dry and hot climate with limited rainfall that impairs the production of several crops. Sesame is a resilient crop that is well suited to this environment. Unfortunately, there is a lack of data relative to the status of its production in West Africa. We made investigations in four major sesame-growing areas of Senegal and Mali, into the status of the crop's production, its agronomic practices, the challenges farmers face and their preferences concerning the traits that should be improved. Results: A total of 256 sesame producers in 47 villages were interviewed using a semi-structured questionnaire. The results showed that sesame is a multi-ethnic crop and only 20% of the total fields owned by farmers were allocated to its cultivation. The yield and the seasonal production of sesame per farmer was quite weak showing that this crop is still a commodity grown on a small scale. Various cultivars were grown, and the most widely grown ones have considerable levels of oil (53–60.34%) and protein (18–21.89%) contents. In both countries, seed marketing was the main impediment the producers faced on account of a lack of reliable markets and of a considerable fluctuation in prices. Conclusions: Overall, the sesame sector is still traditional but is progressively developing and sesame could become an important cash crop for smallholders in West Africa's Sahel. Research programs should target the release of the varieties with higher yield, a stronger resistance to drought, heat, diseases and pests, a good seed quality and improved plant architecture. This study represents the first insight into the sesame sector in West Africa's Sahel, and our findings may guide researchers and policy-makers to boost this sector for ensuring food security and the improvement of small-scale farmers' livelihood.
Drought is one of the most important abiotic stresses that impair sesame (Sesamum indicum L.) pro... more Drought is one of the most important abiotic stresses that impair sesame (Sesamum indicum L.) productivity mainly when it occurs at flowering stage. However up to now, very few studies have attempted to investigate the molecular responses of sesame to drought stress. In this experiment, two genotypes having contrasting responses to drought (tolerant and sensitive) were submitted to progressive drought followed by recovering stage at flowering stage. RNAs were isolated from roots of plants before drought stress, at 3-time points during progressive drought, after rewatering, and sequenced using Illumina HiSeq 4000 platform. These RNA-Seq resources (BioSample IDs: SAMN06130606 and SAMN06130607) provided an opportunity to elucidate the molecular responses of sesame to drought and find out some candidate genes for drought tolerance improvement.
Sesame is a survivor crop cultivated for ages in arid areas under high temperatures and limited w... more Sesame is a survivor crop cultivated for ages in arid areas under high temperatures and limited water conditions. Since its entire genome has been sequenced, revealing evolution, and functional characterization of its abiotic stress genes became a hot topic. In this study, we performed a whole-genome identification and analysis of Hsf gene family in sesame. Thirty genes encoding Hsf domain were found and classified into 3 major classes A, B, and C. The class A members were the most representative one and Hsf genes were distributed in 12 of the 16 linkage groups (except the LG 8, 9, 13, and 16). Evolutionary analysis revealed that, segmental duplication events which occurred around 67 MYA, were the primary force underlying Hsf genes expansion in sesame. Comparative analysis also suggested that sesame has retained most of its Hsf genes while its relatives viz. tomato and potato underwent extensive gene losses during evolution. Continuous purifying selection has played a key role in the maintenance of Hsf genes in sesame. Expression analysis of the Hsf genes in sesame revealed their putative involvement in multiple tissue-/developmental stages. Time-course expression profiling of Hsf genes in response to drought stress showed that 90% Hsfs are drought responsive. We infer that classes B-Hsfs might be the primary regulators of drought response in sesame by cooperating with some class A genes. This is the first insight into this gene family and the results provide some gene resources for future gene cloning and functional studies toward the improvement in stress tolerance of sesame.
Sesame is one of the oldest oilseed crops grown
mainly in Africa and Asia. Although genetic and g... more Sesame is one of the oldest oilseed crops grown mainly in Africa and Asia. Although genetic and genomic studies on sesame have started late, the past 5 years have witnessed extensive progresses in these areas on this crop. Important genomic sequence resources such as functional markers, genes and QTLs linked to agronomically important traits, have been generated through linkage mapping and association analysis to assist sesame improvement programs. However, most of these data are scattered in different maps making them hard to be exploited efficiently in breeding programs. In this study, we report a comprehensive physical map gathering 151 published genomic sequence resources which highlighted some hotspot functional regions in the sesame genome. Moreover, 83,135 non-redundant SSRs have been supplied along with their physical position and motif composition. This will assist future research in fine mapping or pinpointing more functional genes based on the already published QTLs and functional markers. This physical map represents a good landmark for further non-overlapping genetic and genomic studies working towards sesame improvement.
Microsatellite DNAs (or SSRs) are important genomic components involved in many important biologi... more Microsatellite DNAs (or SSRs) are important genomic components involved in many important biological functions. SSRs have been extensively exploited as molecular markers for diverse applications including genetic diversity, linkage/association mapping of gene/QTL, marker-assisted selection, variety identification and evolution analysis. However, a comprehensive database or web service for studying mi-crosatellite DNAs and marker development in plants is lacking. Here, we developed a database, PMD-Base, which integrates large amounts of microsatel-lite DNAs from genome sequenced plant species and includes a web service for microsatellite DNAs identification. In PMDBase, 26 230 099 microsatellite DNAs were identified spanning 110 plant species. Up to three pairs of primers were supplied for every microsatellite DNA. For 81 species, genomic features of the microsatellite DNAs (genic or non-genic) were supplied with the corresponding genes or transcripts from public databases. Microsatellite DNAs can be explored through browsing and searching modules with a user-friendly web interface and cus-tomized software. Furthermore, we developed MIS-Aweb and embedded Primer3web to help users to identify microsatellite DNAs and design corresponding primers in their own genomic sequences online. All datasets of microsatellite DNAs can be down-loaded conveniently. PMDBase will be updated regularly with new available genome data and can be accessed freely via the address http://www.sesame-bioinfo.org/PMDBase.
Sesame (Sesamum indicum L.) is one of the most important oilseed crops. It is mainly grown in ari... more Sesame (Sesamum indicum L.) is one of the most important oilseed crops. It is mainly grown in arid and semi-arid regions with occurrence of unpredictable drought which is one of the major constraints of its production. However, the lack of gene resources associated with drought tolerance hinders sesame genetic improvement towards this osmotic stress. The present research aimed at identifying candidate genes associated with drought tolerance in the whole genome of sesame through homology search of known drought associated genes from three relative species, viz., potato, tomato and the well-described model plant Arabidopsis. Based on 2,495 sequences including 1,150 from Arabidopsis, 1,075 from potato and 270 from tomato, comparative analysis against sesame genome led to the identification of a set of 75 candidate genes (42, 22 and 11 from Arabidopsis, potato and tomato, respectively). Mapping results showed that the candidate genes were distributed on the 16 sesame linkage groups. Wide range of genes with various functions identified in this study confirmed that drought tolerance in sesame is under the control of several genes. Based on their functional classification, 2 groups of candidate genes were identified: (a) genes which protect the plant against drought effect; (b) signal transduction genes and transcription factors. Many transcription factors were retrieved including 6 AP2/ERF genes among which 3 were more expressed in drought tolerant material compared to the sensitive one and might play some important roles in drought tolerance in sesame. Our results provided genomic resources for further functional analysis and genetic engineering towards drought tolerance improvement in sesame.
Background: Sesame is an important oilseed crop mainly grown in inclement areas with high tempera... more Background: Sesame is an important oilseed crop mainly grown in inclement areas with high temperatures and frequent drought. Thus, drought constitutes one of the major constraints of its production. The AP2/ERF is a large family of transcription factors known to play significant roles in various plant processes including biotic and abiotic stress responses. Despite their importance, little is known about sesame AP2/ERF genes. This constitutes a limitation for drought-tolerance candidate genes discovery and breeding for tolerance to water deficit.
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Papers by Komivi Dossa
seed and market value. Variation in sesame seed components including oil, oleic acid,
linoleic acid, and protein was investigated by near-infrared reflectance spectrometry in 139
samples collected from different countries. Oil and protein contents were between 40.8%
and 60.3% (mean 53.0%) and 15.5% to 25.5% (mean 20.4%), respectively. Linoleic acid,
ranging from 31.8% to 52.4% (mean 46%) was more abundant than oleic acid (31.8%–50.6%,
mean 38.1%). Light-seeded samples displayed higher nutritional quality, as they were richer
in oil, protein, and linoleic acid than dark seeds. Samples from Africa had higher oil and
linoleic acid contents, while Asian samples had higher oleic content. The analysis revealed
West African sesame cultivars containing especially high levels of seed components, which
may command high market values. Two clusters of sesame samples grouped by seed
composition were obtained, including one cluster with high oil and oleic acid content and
the other with high protein and linoleic acid content. This study revealed that sesame
samples from Africa and Asia harbor high variation for major seed components and also
provided background information for breeding high-nutrition varieties according to the
demands of sesame seed markets.
mainly in Africa and Asia. Although genetic and genomic
studies on sesame have started late, the past 5 years have
witnessed extensive progresses in these areas on this crop.
Important genomic sequence resources such as functional
markers, genes and QTLs linked to agronomically important
traits, have been generated through linkage mapping
and association analysis to assist sesame improvement
programs. However, most of these data are scattered in
different maps making them hard to be exploited efficiently
in breeding programs. In this study, we report a comprehensive
physical map gathering 151 published genomic
sequence resources which highlighted some hotspot functional
regions in the sesame genome. Moreover, 83,135
non-redundant SSRs have been supplied along with their
physical position and motif composition. This will assist
future research in fine mapping or pinpointing more functional
genes based on the already published QTLs and
functional markers. This physical map represents a good
landmark for further non-overlapping genetic and genomic
studies working towards sesame improvement.
seed and market value. Variation in sesame seed components including oil, oleic acid,
linoleic acid, and protein was investigated by near-infrared reflectance spectrometry in 139
samples collected from different countries. Oil and protein contents were between 40.8%
and 60.3% (mean 53.0%) and 15.5% to 25.5% (mean 20.4%), respectively. Linoleic acid,
ranging from 31.8% to 52.4% (mean 46%) was more abundant than oleic acid (31.8%–50.6%,
mean 38.1%). Light-seeded samples displayed higher nutritional quality, as they were richer
in oil, protein, and linoleic acid than dark seeds. Samples from Africa had higher oil and
linoleic acid contents, while Asian samples had higher oleic content. The analysis revealed
West African sesame cultivars containing especially high levels of seed components, which
may command high market values. Two clusters of sesame samples grouped by seed
composition were obtained, including one cluster with high oil and oleic acid content and
the other with high protein and linoleic acid content. This study revealed that sesame
samples from Africa and Asia harbor high variation for major seed components and also
provided background information for breeding high-nutrition varieties according to the
demands of sesame seed markets.
mainly in Africa and Asia. Although genetic and genomic
studies on sesame have started late, the past 5 years have
witnessed extensive progresses in these areas on this crop.
Important genomic sequence resources such as functional
markers, genes and QTLs linked to agronomically important
traits, have been generated through linkage mapping
and association analysis to assist sesame improvement
programs. However, most of these data are scattered in
different maps making them hard to be exploited efficiently
in breeding programs. In this study, we report a comprehensive
physical map gathering 151 published genomic
sequence resources which highlighted some hotspot functional
regions in the sesame genome. Moreover, 83,135
non-redundant SSRs have been supplied along with their
physical position and motif composition. This will assist
future research in fine mapping or pinpointing more functional
genes based on the already published QTLs and
functional markers. This physical map represents a good
landmark for further non-overlapping genetic and genomic
studies working towards sesame improvement.