Guoping Fan
University of California, Los Angeles, Human Genetics, Faculty Member
Here we use a systems biology approach to comprehensively assess the conservation of gene networks in naive pluripotent stem cells (PSCs) with preimplantation embryos. While gene networks in murine naive and primed pluripotent states are... more
Here we use a systems biology approach to comprehensively assess the conservation of gene networks in naive pluripotent stem cells (PSCs) with preimplantation embryos. While gene networks in murine naive and primed pluripotent states are reproducible across data sets, different sources of human stem cells display high degrees of variation, partly reflecting disparities in culture conditions. Finally, naive gene networks between human and mouse PSCs are not well conserved and better resemble their respective blastocysts.
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ABSTRACT
Research Interests:
1. Linze Inland River Basin Research Station & Laboratory of Heibe River Eco-Hydrology and Basin Science, Cold and Arid Regions Environmental and Engineering Research Institute, Chinese Academy of Sciences, Lanzhou 730000, China; 2. Qilian Shan Station of Glaciology and Ecologic Environment, Stat...more
Research Interests: Kinetics, Linear models, Congenital Heart Defects, Osteoporosis, Humans, and 27 moreFemale, Male, Pediatric Surgery, Regression Analysis, Infant, Bone Density, Body Mass Index, Risk factors, Triglycerides, Newborn Infant, Aged, Middle Aged, Coronary Angiography, Clinical Cardiology, Risk Factors, Soft Tissue, Indexation, Coronary Artery Disease, Clinical Presentation, Reference Values, Enzyme Linked Immunosorbent Assay, Capillary Leak Syndrome, Predictive value of tests, Vascular Endothelial Growth Factor, Case Control Studies, Postoperative care, and Cardiopulmonary bypass
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DNA methyltransferase I (Dnmt1), the maintenance enzyme for DNA cytosine methylation, is expressed at high levels in the CNS during embryogenesis and after birth. Because embryos deficient for Dnmt1 die at gastrulation, the role of Dnmt1... more
DNA methyltransferase I (Dnmt1), the maintenance enzyme for DNA cytosine methylation, is expressed at high levels in the CNS during embryogenesis and after birth. Because embryos deficient for Dnmt1 die at gastrulation, the role of Dnmt1 in the development and function of the nervous system could not be studied by using this mutation. We therefore used the cre/loxP system to
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Research Interests:
The mechanisms by which neural stem cells give rise to neurons, astrocytes, or oligodendrocytes are beginning to be elucidated. However, it is not known how the specification of one cell lineage results in the suppression of alternative... more
The mechanisms by which neural stem cells give rise to neurons, astrocytes, or oligodendrocytes are beginning to be elucidated. However, it is not known how the specification of one cell lineage results in the suppression of alternative fates. We find that in addition to inducing ...
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Research Interests: Genomic Imprinting, Biological Chemistry, Transcription Factors, Gene Silencing, Biological Sciences, and 13 moreHumans, Protein Kinases, Mice, Animals, Histone Modification, Biological, DNA methylation, CpG islands, CHEMICAL SCIENCES, Histones, Lysine, Chromatin Immunoprecipitation, and Cyclophilin A
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Research Interests:
Research Interests: Analytical Chemistry, Mass Spectrometry, Induced Pluripotent Stem Cells (I Psc), Humans, Mice, and 19 moreAnimals, Embryonic Stem Cell, DNA methylation, Analytical, Tandem Mass Spectrometry, Embryonic Stem Cells, High Performance Liquid Chromatography, Genomic DNA, Liquid Chromatography, Reproducibility of Results, Analytical Biochemistry, Induced Pluripotent Stem Cells, Hydroxylation, Human Fibroblasts, Thin Layer Chromatography, DNA binding proteins, Quantitative Method, Biochemistry and cell biology, and 5-Methylcytosine
Embryonic stem cells have the unique ability to indefinitely self-renew and differentiate into any cell type found in the adult body. Differentiated cells can, in turn, be reprogrammed to embryonic stem-like induced pluripotent stem... more
Embryonic stem cells have the unique ability to indefinitely self-renew and differentiate into any cell type found in the adult body. Differentiated cells can, in turn, be reprogrammed to embryonic stem-like induced pluripotent stem cells, providing exciting opportunities for achieving patient-specific stem cell therapy while circumventing immunological obstacles and ethical controversies. Since both differentiation and reprogramming are governed by major changes in the epigenome, current directions in the field aim to uncover the epigenetic signals that give pluripotent cells their unique properties. DNA methylation is one of the major epigenetic factors that regulates gene expression in mammals and is essential for establishing cellular identity. Recent analyses of pluripotent and somatic cell methylomes have provided important insights into the extensive role of DNA methylation during cell-fate commitment and reprogramming. In this article, the recent progress of differentiation and reprogramming research illuminated by high-throughput studies is discussed in the context of DNA methylation.