Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Jan 6, 2016
Rabies has been an enigmatic disease because microscopic findings in central nervous system tissu... more Rabies has been an enigmatic disease because microscopic findings in central nervous system tissues do not always correlate well with the severity of the clinical illness. Immunohistochemical staining of the calcium-binding protein calbindin (specifically CbD28k) seems to be the technique most used to identify Purkinje neurons under normal and pathological conditions. In the present work, we evaluated CbD28k immunoreactivity in the cerebellar cortex of normal and natural Rabies virus (RABV)-infected cattle. We examined brains from 3 normal cows and from 6 crossbreed cattle with a histologic diagnosis of rabies. Samples were taken from the cerebral cortex, cerebellum, hippocampus, and brainstem. Immunohistochemistry was carried out using the following primary antibodies: anti-RABV, anti-GFAP, and anti-CbD28k. In the cerebellar cortex, RABV infection caused the loss of CbD28k immunostaining in Purkinje cells; some large interneurons in the granular layer maintained their positive CbD2...
We report infection by Ichthyophonus sp. in a South American amphibian, the hylid frog Hypsiboas ... more We report infection by Ichthyophonus sp. in a South American amphibian, the hylid frog Hypsiboas pulchellus in Uruguay. This frog had a large subcutaneous mass over the urostyle and dorsal musculature comprised of parasitic cysts with mild granulomatous inflammation but otherwise appeared healthy.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Jan 6, 2016
Rabies has been an enigmatic disease because microscopic findings in central nervous system tissu... more Rabies has been an enigmatic disease because microscopic findings in central nervous system tissues do not always correlate well with the severity of the clinical illness. Immunohistochemical staining of the calcium-binding protein calbindin (specifically CbD28k) seems to be the technique most used to identify Purkinje neurons under normal and pathological conditions. In the present work, we evaluated CbD28k immunoreactivity in the cerebellar cortex of normal and natural Rabies virus (RABV)-infected cattle. We examined brains from 3 normal cows and from 6 crossbreed cattle with a histologic diagnosis of rabies. Samples were taken from the cerebral cortex, cerebellum, hippocampus, and brainstem. Immunohistochemistry was carried out using the following primary antibodies: anti-RABV, anti-GFAP, and anti-CbD28k. In the cerebellar cortex, RABV infection caused the loss of CbD28k immunostaining in Purkinje cells; some large interneurons in the granular layer maintained their positive CbD2...
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Jan 21, 2015
Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perik... more Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, β-tubulin, and affinity reaction aga...
Journal of Animal Physiology and Animal Nutrition, 2013
The energetic status of high-yielding Holstein-Friesian dairy cattle was studied during peripartu... more The energetic status of high-yielding Holstein-Friesian dairy cattle was studied during peripartum under field conditions using body condition score (BCS), glycemia, seric ß-hydroxybutyrate and adipose tissue cellularity. This last method was tested as a complementary tool for energetic status assessment. Biopsies of pericaudal subcutaneous adipose tissue were obtained from 25 multiparous animals at 28 days before and 21 days after parturition. Samples were routinely processed for histological examination and stained with haematoxylin and eosin (H&E). The mean diameter of adipocytes (MDA) was measured with the aid of a digital image processor. During the same period, blood samples were collected weekly for metabolite determinations. The MDA at 28 days pre-partum and 21 days post-partum were 72.1 vs. 66.2 μm respectively (p = 0.055), and the corresponding BCS at these moments was 3.32 vs. 3.19 (p = 0.068). At -28 days pre-partum, the BCS was positively correlated with MDA (Pearson's r = 0.521, p = 0.016) and with glycemia (Pearson's r = 0.404, p = 0.056). Correlations between BCS and MDA, and between BCS and glycemia, with ß-hydroxybutyrate although not significant, suggest that routine histological preparations of biopsies from subcutaneous adipose tissue could be included as an easy and valuable tool for research purposes to evaluate metabolic adaptation of dairy cows to peripartum, as well as the incidence of metabolic disorders and productive performance.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Jan 6, 2016
Rabies has been an enigmatic disease because microscopic findings in central nervous system tissu... more Rabies has been an enigmatic disease because microscopic findings in central nervous system tissues do not always correlate well with the severity of the clinical illness. Immunohistochemical staining of the calcium-binding protein calbindin (specifically CbD28k) seems to be the technique most used to identify Purkinje neurons under normal and pathological conditions. In the present work, we evaluated CbD28k immunoreactivity in the cerebellar cortex of normal and natural Rabies virus (RABV)-infected cattle. We examined brains from 3 normal cows and from 6 crossbreed cattle with a histologic diagnosis of rabies. Samples were taken from the cerebral cortex, cerebellum, hippocampus, and brainstem. Immunohistochemistry was carried out using the following primary antibodies: anti-RABV, anti-GFAP, and anti-CbD28k. In the cerebellar cortex, RABV infection caused the loss of CbD28k immunostaining in Purkinje cells; some large interneurons in the granular layer maintained their positive CbD2...
We report infection by Ichthyophonus sp. in a South American amphibian, the hylid frog Hypsiboas ... more We report infection by Ichthyophonus sp. in a South American amphibian, the hylid frog Hypsiboas pulchellus in Uruguay. This frog had a large subcutaneous mass over the urostyle and dorsal musculature comprised of parasitic cysts with mild granulomatous inflammation but otherwise appeared healthy.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Jan 6, 2016
Rabies has been an enigmatic disease because microscopic findings in central nervous system tissu... more Rabies has been an enigmatic disease because microscopic findings in central nervous system tissues do not always correlate well with the severity of the clinical illness. Immunohistochemical staining of the calcium-binding protein calbindin (specifically CbD28k) seems to be the technique most used to identify Purkinje neurons under normal and pathological conditions. In the present work, we evaluated CbD28k immunoreactivity in the cerebellar cortex of normal and natural Rabies virus (RABV)-infected cattle. We examined brains from 3 normal cows and from 6 crossbreed cattle with a histologic diagnosis of rabies. Samples were taken from the cerebral cortex, cerebellum, hippocampus, and brainstem. Immunohistochemistry was carried out using the following primary antibodies: anti-RABV, anti-GFAP, and anti-CbD28k. In the cerebellar cortex, RABV infection caused the loss of CbD28k immunostaining in Purkinje cells; some large interneurons in the granular layer maintained their positive CbD2...
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, Jan 21, 2015
Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perik... more Intoxication with Solanum bonariense in cattle causes cerebellar cortical degeneration with perikaryal vacuolation, axonal swelling, and death primarily of Purkinje cells, with accumulation of electron-dense residual storage bodies in membrane-bound vesicles. The pathogenesis of this disease is not fully understood. Previously, we proposed that inhibition of protein synthesis in Purkinje cells among other altered metabolic pathways could lead to cytoskeletal alterations, subsequently altering cell-specific axonal transport. In the present study, immunohistochemical and histochemical methods were used to identify neuronal cytoskeletal alterations and axonal loss, demyelination, and astrogliosis in the cerebellum of intoxicated bovines. Samples of cerebellum from 3 natural and 4 experimental cases and 2 control bovines were studied. Immunoreactivity against neurofilament (NF)-200KDa confirmed marked loss of Purkinje neurons, and phospho-NF protein, β-tubulin, and affinity reaction aga...
Journal of Animal Physiology and Animal Nutrition, 2013
The energetic status of high-yielding Holstein-Friesian dairy cattle was studied during peripartu... more The energetic status of high-yielding Holstein-Friesian dairy cattle was studied during peripartum under field conditions using body condition score (BCS), glycemia, seric ß-hydroxybutyrate and adipose tissue cellularity. This last method was tested as a complementary tool for energetic status assessment. Biopsies of pericaudal subcutaneous adipose tissue were obtained from 25 multiparous animals at 28 days before and 21 days after parturition. Samples were routinely processed for histological examination and stained with haematoxylin and eosin (H&E). The mean diameter of adipocytes (MDA) was measured with the aid of a digital image processor. During the same period, blood samples were collected weekly for metabolite determinations. The MDA at 28 days pre-partum and 21 days post-partum were 72.1 vs. 66.2 μm respectively (p = 0.055), and the corresponding BCS at these moments was 3.32 vs. 3.19 (p = 0.068). At -28 days pre-partum, the BCS was positively correlated with MDA (Pearson's r = 0.521, p = 0.016) and with glycemia (Pearson's r = 0.404, p = 0.056). Correlations between BCS and MDA, and between BCS and glycemia, with ß-hydroxybutyrate although not significant, suggest that routine histological preparations of biopsies from subcutaneous adipose tissue could be included as an easy and valuable tool for research purposes to evaluate metabolic adaptation of dairy cows to peripartum, as well as the incidence of metabolic disorders and productive performance.
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Papers by J. Verdes