mohammd alrufae

The biological methods for nanoparticles synthesis is vital area due to their benefits over chemical and physical methods of synthesis. The aim of the present study is the biosynthesis of silver nanoparticles using Pantoea agglomerans and evaluating their biomedical activity. AgNPs were biosynthesized by adding AgNO 3 to cell free supernatant of P.agglomerans at concentration (10 mM). The PCR was accomplished to molecular identification of P. agglomerans using specific primers for 16S rRNA gene, foreword primer (8fpl) and reverse primer (1492rpl) Biosynthesis of AgNPs was firstly indicated by the colour alteration of reaction mixture from yellow to reddish brown. The characterization completed by XRD, SEM, EDS, and AFM. The XRD presented the size of AgNPs was 16nm.The SEM was offered the shape was spherical and homogenous and the size was ranged between 64-100nm. The occurrence of elemental silver was analysed by EDS. The AFM displayed three dimensional structures of silver nanoparticles and the average diameter was 98.78 nm. Biosynthesized AgNPs showed antibacterial activity against multidrug resistant bacteria (MDR) of both gram positive and gram negative bacteria (S.aureus, S.pyogenes, E.coli, K.pneumoniae, E.aerogens ,S.typhi, A.baumannii, P.auroginosa and P.mirabilis). All tested bacterial isolates revealed their ability to form biofilm in the form of biofilm using tube and Congo red method without treated by the nanoparticles except P.mirabils, A.boumanii and E.aerogenes ,but when treated with AgNPs this ability was prevented and removed in K.pneumoniae, Ps.auroginosa and C.neoformans The AgNPs at two concentration 1mg/ml and 2mg/ml bared their antioxidant capacity in vitro by scavenging DPPH free radicles, the largest inhibition titer found in the mixture of DPPH with biogenic AgNPs at concentration 2mg/ml (87.97).