Carlos Eduardo P. Corbett
Universidade de São Paulo, Pathology, Faculty Member
During the natural transmission of Leishmania parasites, the infected sand fly female regurgitates promastigotes into the host's skin together with its saliva. It has been reported that vector saliva contains immunomodulatory... more
During the natural transmission of Leishmania parasites, the infected sand fly female regurgitates promastigotes into the host's skin together with its saliva. It has been reported that vector saliva contains immunomodulatory molecules that facilitate the establishment of infection. Thus, the main objective of this study was to evaluate the specificity of Lutzomyia (Lu.) flaviscutellata and Lu. (Psychodopygus) complexus salivas on the infectivity of Leishmania (L.) (Leishmania) amazonensis and L. (Viannia) braziliensis, respectively. BALB/c mice were inoculated into the skin of hind footpad with L. (L.) amazonensis and L. (V.) braziliensis promastigotes in the absence or presence of Lu. flaviscutellata and Lu. (P.) complexus salivary gland homogenates (SGHs). The evolution of the infection was evaluated by lesion size, histopathological analysis and determination of the parasite load in the skin biopsies collected from the site of infection at 4 and 8 weeks PI. The lesion size a...
Research Interests:
Species of Baccharis exhibit antibiotic, antiseptic, and wound-healing properties, and have been used in the traditional medicine of South America for the treatment of inflammation, headaches, diabetes, and hepatobiliary disorders. To... more
Species of Baccharis exhibit antibiotic, antiseptic, and wound-healing properties, and have been used in the traditional medicine of South America for the treatment of inflammation, headaches, diabetes, and hepatobiliary disorders. To investigate the anti-inflammatory activity of organic phases from EtOH extract of the aerial parts of Baccharis uncinella DC (Asteraceae). The crude EtOH extract from the aerial parts of B. uncinella was subjected to partition procedures and the corresponding CH(2)Cl(2) and EtOAc phases were subjected to several chromatographic separation procedures. Thus, these phases and their purified compounds were assayed for evaluation of anti-inflammatory activity. The CH(2)Cl(2) phase from EtOH extract from B. uncinella contained two triterpenoids (oleanolic and ursolic acids) and one flavonoid (pectolinaringenin), whereas the respective EtOAc phase showed to be composed mainly by two phenylpropanoid derivatives (caffeic and ferulic acids). The CH(2)Cl(2) and EtOAc phases as well as their isolated compounds exhibited anti-inflammatory effects against inflammatory reactions induced by phospholipase A2 (from Crotalus durissus terrificus venom) and by carrageenan. The results suggested that the components obtained from partition phases of EtOH extract of B. uncinella could represent lead molecules for the development of anti-inflammatory agents. Additionally, the results confirmed the use of Baccharis genus in the traditional medicine of South America for the treatment of inflammation and other heath disorders. To date, the present work describes for the first time the anti-inflammatory effects of compounds isolated from B. uncinella.
Research Interests:
Research Interests: Microbiology, Parasitology, Traditional Medicine, Medical Microbiology, Wound Healing, and 16 moreMedicinal Plants, Macrophages, Magnetic Resonance Spectroscopy, Cell line, Mice, Nitric oxide, Animals, Nitrates, South America, Ursolic Acid, Plant extracts, Veterinary Sciences, Chromones, Oleanolic acid, Baccharis, and Triterpenes
Research Interests:
Paraffin-embedded samples commonly stored at educational and research institutions constitute tissues banks for follow-up or epidemiological studies; however, the paraffin inclusion process involves the use of substances that can cause... more
Paraffin-embedded samples commonly stored at
educational and research institutions constitute tissues banks
for follow-up or epidemiological studies; however, the
paraffin inclusion process involves the use of substances that
can cause DNA degradation. In this study, a PCR protocolwas
applied to identify Leishmania strains in 33 paraffinembedded
skin samples of patients with American cutaneous
leishmaniasis. DNA was obtained by the phenol-chloroform
protocol following paraffin removal and then used in PCR or
nested PCR based on the nucleotide sequence of the small
subunit ribosomal RNA (SSU rDNA). The amplicons
obtained were cloned and sequenced to determine the single
nucleotide polymorphism that distinguishes between different
Leishmania species or groups. This assay allowed to
distinguish organisms belonging to the subgenus Viannia and
identify L. (Leishmania) amazonensis and L. (L.) chagasi of
the Leishmania subgenus. Of the 33 samples, PCR and
nested PCR identified 91% of samples. After sequencing the
PCR product of 26 samples, 16 were identified as L. (L.)
amazonensis, the other 10 contain organisms belonging to
the L. (Viannia) sub-genus. These results open a huge
opportunity to study stored samples and promote relevant
contributions to epidemiological studies.
educational and research institutions constitute tissues banks
for follow-up or epidemiological studies; however, the
paraffin inclusion process involves the use of substances that
can cause DNA degradation. In this study, a PCR protocolwas
applied to identify Leishmania strains in 33 paraffinembedded
skin samples of patients with American cutaneous
leishmaniasis. DNA was obtained by the phenol-chloroform
protocol following paraffin removal and then used in PCR or
nested PCR based on the nucleotide sequence of the small
subunit ribosomal RNA (SSU rDNA). The amplicons
obtained were cloned and sequenced to determine the single
nucleotide polymorphism that distinguishes between different
Leishmania species or groups. This assay allowed to
distinguish organisms belonging to the subgenus Viannia and
identify L. (Leishmania) amazonensis and L. (L.) chagasi of
the Leishmania subgenus. Of the 33 samples, PCR and
nested PCR identified 91% of samples. After sequencing the
PCR product of 26 samples, 16 were identified as L. (L.)
amazonensis, the other 10 contain organisms belonging to
the L. (Viannia) sub-genus. These results open a huge
opportunity to study stored samples and promote relevant
contributions to epidemiological studies.