Despite high-dose chemotherapy and autografting, the outcome for patients with primary refractory Hodgkin’s disease (HD) or multiple relapses remains unsatisfactory. Six pediatric patients (median age: 16 years, range: 11–19) received... more
Despite high-dose chemotherapy and autografting, the outcome for patients with primary refractory Hodgkin’s disease (HD) or multiple relapses remains unsatisfactory. Six pediatric patients (median age: 16 years, range: 11–19) received reduced intensity conditioning and allogeneic peripheral blood stem cell transplantation (PBSCT) after failure of stratified first-line chemotherapy, involved-field radiotherapy, and salvage chemotherapy including autologous PBSCT (two patients). For conditioning, fludarabine was
Research Interests: Surgery, Chemotherapy, Treatment Outcome, Adolescent, Medicine, and 15 moreTransplantation, Humans, Child, Internal Medicine, Female, Male, Disease Progression, Cyclophosphamide, Antineoplastic Agents, Hodgkin disease, Combined Modality Therapy, Immunosuppressive Agents, Busulfan, Vidarabine, and Cardiovascular medicine and haematology
ResumoO fator estimulador de células granulocitárias (G-CSF) é uma glicoproteína descrita há mais de 20 anos, possui aprovação da FDA (Food and Drug Administration) e do Ministério da Saúde no Brasil para tratamento de estados... more
ResumoO fator estimulador de células granulocitárias (G-CSF) é uma glicoproteína descrita há mais de 20 anos, possui aprovação da FDA (Food and Drug Administration) e do Ministério da Saúde no Brasil para tratamento de estados neutropênicos e no transplante de medula óssea. O G-CSF estimula os precursores dos granulócitos e regula crucialmente a sobrevivência de neutrófilos maduros, pós-mitóticos, por meio da inibição da apoptose. Além do efeito sistêmico, mais recentemente, tem-se demonstrado uma surpreendente atividade do G-CSF no sistema nervoso central. A administração de G-CSF mobiliza células-tronco progenitoras da medula óssea para o sangue periférico, atravessa a barreira hematoencefálica (BHE) e se dirige à área acometida do cérebro. A atividade do G-CSF no sistema nervoso central tem sido caracterizada como multimodal, pois, além do efeito mobilizador de células da medula óssea, demonstrou-se uma ação direta neuroprotetora mediante diferentes mecanismos, tais como a ativid...
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Autoimmune diseases are an important field for the development of hematopoietic stem cell transplantation (HSCT). The Brazilian Society for Cellular Therapy and Bone Marrow Transplantation (Sociedade Brasileira de Terapia Celular e... more
Autoimmune diseases are an important field for the development of hematopoietic stem cell transplantation (HSCT). The Brazilian Society for Cellular Therapy and Bone Marrow Transplantation (Sociedade Brasileira de Terapia Celular e Transplante de Medula Óssea, SBTMO) organized consensus meetings for the Autoimmune Diseases Group, to review the available literature on HSCT for autoimmune diseases, aiming to gather data that support the procedure for these patients. Three autoimmune diseases for which there are evidence-based indications for HSCT are multiple sclerosis, systemic sclerosis and Crohn’s disease. The professional stem cell transplant societies in North America (ASTCT), Europe (EBMT) and Brazil (SBTMO) currently consider HSCT as a therapeutic modality for these three autoimmune diseases. The data are here updated.
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Body iron disorders have been reported after myeloablative conditioning in patients undergoing hematopoietic stem cell transplantation (HSCT). There is a concern that labile plasma iron (LPI), the redox-active form of iron, can be... more
Body iron disorders have been reported after myeloablative conditioning in patients undergoing hematopoietic stem cell transplantation (HSCT). There is a concern that labile plasma iron (LPI), the redox-active form of iron, can be involved in the occurrence of toxicity and other complications commonly observed in the early post-HSCT period. In order to better understand the LPI kinetics and its determinants and implications, we undertook sequential LPI determinations before and after conditioning until engraftment in 25 auto-HSCT patients. Increased LPI was present in only 5 patients before starting conditioning. Shortly after conditioning, LPI levels were increased in 23 patients, with peak at day 0, returning to normal range upon engraftment in 21 patients. Overall, LPI levels correlated weakly with serum ferritin and more strongly with transferrin saturation; however, both parameters were apparently not applicable as surrogate markers for increased LPI. Although this was a small ...
Research Interests: Chemistry, Medicine, Antioxidants, Humans, Internal Medicine, and 15 moreFemale, Male, Iron, Haematopoiesis, Ferritin, Iron Overload, Aged, Middle Aged, Ascorbic Acid, Adult, Conditioning, Cohort Studies, Hematopoietic Stem Cell Transplantation, fluorescent dyes, and Cardiovascular medicine and haematology
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Additional file 3. Hematological abnormalities during autologous hematopoietic stem cell transplantation.
Additional file 1. Sociodemographic and clinical characteristics.
Crohn’s disease (CD) is an inflammatory bowel disease that can affect any site of the digestive system. It occurs due to an immunological imbalance and is responsible for intestinal mucosal lesions and complications such as fistulas and... more
Crohn’s disease (CD) is an inflammatory bowel disease that can affect any site of the digestive system. It occurs due to an immunological imbalance and is responsible for intestinal mucosal lesions and complications such as fistulas and stenoses. Treatment aims to stabilize the disease, reducing the symptoms and healing intestinal lesions. Surgical procedures are common in patients. Cell therapy was initially used to treat this disease in patients who also suffered from lymphoma and leukemia and were considered to be good candidates for autologous and allogeneic transplantation. After transplantation, an improvement was also observed in their CD. In 2003, the procedure began to be used to treat the disease itself, and several case series and randomized studies have been published since then; this approach currently comprises a new option in the treatment of CD. However, considerable doubt along with significant gaps in our knowledge continue to exist in relation to cell therapy for ...
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CONTEXT: Epidemiological studies have demonstrated higher frequencies of the O blood group and the non-secretor phenotype of ABH antigens among patients suffering from peptic ulcers. Since Helicobacter pylori has been established as the... more
CONTEXT: Epidemiological studies have demonstrated higher frequencies of the O blood group and the non-secretor phenotype of ABH antigens among patients suffering from peptic ulcers. Since Helicobacter pylori has been established as the main etiological factor in this disease, controversies about the associations of the ABO and Lewis blood group phenotypes and secretor and non-secretor phenotypes in relation to susceptibility towards infection by this bacillus have been presented. OBJECTIVE: To verify the frequencies of ABO, Lewis blood group phenotypes, secretor and non-secretor phenotypes in patients infected or uninfected by H. pylori. DESIGN: Cross-sectional study. SETTING: Outpatient clinic. PARTICIPANTS: One hundred and twenty patients with dyspeptic symptoms who underwent endoscopy.MAIN MEASUREMENTS: ABO and Lewis blood group phenotypes were determined by a standard hemagglutination test and the secretor and non-secretor phenotypes were evaluated by saliva samples using the i...
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Foram analisados 496 indivíduos de Cubatão, SP (Brasil), cidade com alto teor de poluição industrial, com o objetivo de verificar alterações hematológicas induzidas por poluentes industriais. Dos estudos citológicos dos eritrócitos dessa... more
Foram analisados 496 indivíduos de Cubatão, SP (Brasil), cidade com alto teor de poluição industrial, com o objetivo de verificar alterações hematológicas induzidas por poluentes industriais. Dos estudos citológicos dos eritrócitos dessa população estudada, foram observadas 188 (38%) com alterações, isoladas ou combinadas em um único indivíduo, das quais 26% apresentaram policromatofilia, 24% com pontilhados basófilos, 15% com corpos de Heinz, e 8% com reticulocitose. As freqüências de metahemoglobinemia e sulfohemoglobinemia foram,respectivamente, de 35% e 32% em moradores da vila Parisi - um bairro cercado pela maioria das indústrias de Cubatão - 15% e 5% em operários das indústrias, e 12% e 4% em habitantes de áreas distantes entre 3 e 8 km do polo industrial. Esses resultados indicam que as alterações são causadas por poluentes tóxico-oxidantes e que as conseqüências fisio-patológicas no sangue dos moradores de Cubatão parecem indicar que estão relacionadas com o tempo de exposi...
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Objective The incidence of adverse events in myeloablative transplant protocols is high in refractory Crohn’s disease; this study used low doses of cyclophosphamide. Fourteen patients were submitted to non-myeloablative autologous... more
Objective The incidence of adverse events in myeloablative transplant protocols is high in refractory Crohn’s disease; this study used low doses of cyclophosphamide. Fourteen patients were submitted to non-myeloablative autologous hematopoietic stem cell transplantation. Results The average number of days of anemia (hemoglobin
Objectives This study aimed to evaluate the frequencies of the angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) and methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphisms in obese patients with and without... more
Objectives This study aimed to evaluate the frequencies of the angiotensin converting enzyme (ACE) gene insertion/deletion (I/D) and methylenetetrahydrofolate reductase (MTHFR) gene C677T polymorphisms in obese patients with and without type 2 diabetes mellitus (T2DM). Subjects and methods These polymorphisms were analyzed by polymerase chain reaction in 125 patients with obesity, 47 (T2DM) and 78 (Control Group). Results No significant difference was found on comparing the T2DM and Control Groups in respect to the genotypic frequencies of the polymorphisms - (II: 13.3% vs. 12.0%; ID: 37.8% vs. 37.3; DD: 48.9% vs. 50.7%; CC: 36.2% vs. 39.0%; CT: 46.8% vs. 49.3%; TT: 17.0% vs. 11.7%), and alleles (I: 32.2% vs. 30.7%; D: 67.8% vs. 69.3%; C: 59.6% vs. 63.6%; T: 40.4% vs. 36.4%) and their synergisms in the pathophysiology of T2DM. On analyzing the T2DM Group, there were no significant differences in the presence of complications. In this population of Brazilian obese patients, no correl...
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Resumo: 2281 sangues de cordäo de recém-nascidos da cidade de Santos, Säo Paulo, foram analisados através da eletroforese em gel de ágar amido. A amostra era constituída de 1181 recém-nascidos de mäes negróides e 1100 de mäes consideradas... more
Resumo: 2281 sangues de cordäo de recém-nascidos da cidade de Santos, Säo Paulo, foram analisados através da eletroforese em gel de ágar amido. A amostra era constituída de 1181 recém-nascidos de mäes negróides e 1100 de mäes consideradas caucasóides. ...
ABSTRACT The heart failure (HF) is a syndrome in which the heart becomes incapable of supplying enough oxygen to the tissues. The case of a 42-year old male patient with idiopathic dilated myocardiopathy (ECG – sinus rhythm with typical... more
ABSTRACT The heart failure (HF) is a syndrome in which the heart becomes incapable of supplying enough oxygen to the tissues. The case of a 42-year old male patient with idiopathic dilated myocardiopathy (ECG – sinus rhythm with typical left bundle branch block and duration of QRS=160msec), progressive worsening of clinical history, going through a period of 24 months from functional class II to IV, in spite of the therapeutic optimization until becoming refract. One month later, Gated showed Left Volume Ejection Fraction (LVEF) of 18%, with moderate assynchronism between right / left ventricle and left interventricle. The artificial cardiac resynchronization implant on was realized and followed for an autologous bone marrow stem-cells (BMSCs) via intracoronary. While the hemodynamicist was getting ready for the implant of the same cells in the right coronary artery, the patient developed ventricle fibrillation rhythm with the need of exam suspension for cardiorespiratory resuscitation. Gated showed a little improvement of LVEF (22%), however, an interesting fact was observed: the walls that received the cells had a significant response with contraction improvement which did not reflect in the overall outcome because of the worsening of the walls irrigated by the right coronary artery that did not receive the cells.