HAL Id: hal-02356667 https://hal.science/hal-02356667 Submitted on 2 Jun 2020 HAL is a multi-disciplinary open access archive for the deposit and dissemination of sci- entific research documents, whether they are pub- lished or not. The documents may come from teaching and research institutions in France or abroad, or from public or private research centers. L’archive ouverte pluridisciplinaire HAL, est destinée au dépôt et à la diffusion de documents scientifiques de niveau recherche, publiés ou non, émanant des établissements d’enseignement et de recherche français ou étrangers, des laboratoires publics ou privés. Lipase activity on milk fat globules in a mini-digester to simulate neonatal gastro-intestinal digestion using DISCO beamline Claire Bourlieu-Lacanal, Amélie Deglaire, Olivia Ménard, Thomas Croguennec, Stéphane Pezennec, Juliane Floury, Steven Le Feunteun, Pierre Villeneuve, Frédéric Carrière, Didier Dupont, et al. To cite this version: Claire Bourlieu-Lacanal, Amélie Deglaire, Olivia Ménard, Thomas Croguennec, Stéphane Pezennec, et al.. Lipase activity on milk fat globules in a mini-digester to simulate neonatal gastro-intestinal digestion using DISCO beamline. 14. GERLI Lipidomics meeting, Sep 2018, Saint Maximin la Sainte Beaumes, France. hal-02356667
Lipase activity on milk fat globules in a mini-digester to simulate neonatal gastro-intestinal digestion using DISCO beamline Bourlieu C. 1,3 *, Deglaire A. 1 , Ménard O. 1 , Croguennec T. 1 , Pezennec S. 1 , Floury J. 1 , Le Feunteun S. 2 , Villeneuve P. 3 , Carrière F. 4 , Dupont D. 1 , Bouhallab S. 1 , Fattaciolli J. 5 , Jamme F. 6 , Vié V 7 1 INRA-Agrocampus Ouest UMR 1253 STLO, France; 2 UMR 782 INRA-AgroParisTech GMPA, France; 3 CIRAD/INRA, UMR 1208 IATE, France; 4 CNRS, Aix Marseille University, UMR 7282, France; 5 ENS UMPC UMR CNRS 8640, France ; 6 Synchrotron SOLEIL, France; 7 IPR Institute of Physics, Rennes University 1, France; *claire.bourlieu-lacanal@inra.fr In their native state, milk lipids are present in the form of dispersed droplets called Milk Fat Globules (MFG, diameter 0.1-20 μm, average = 4 μm in bovine milk) [1,2]. The native MFG is constituted by a triglyceride core covered with an external trilayered membrane inherited from its secretory past. This membrane is mainly based on polar lipids (glycerophospholipids, sphingolipids and glycosphingolipids), proteins (25 % of total membrane with high proportion of glycoproteins and enzymes), neutral lipids and minor components. The major biological function of the MFG is to deliver energy to the mammal newborn, for that, the MFG has to be hydrolyzed in the gastro-intestinal tract by lipases, in successive steps. The lipases should interact with the MFG membrane and diffuse in the supramolecular object. The aim of this work is to determine the mechanism involved in the enzymatic hydrolysis along the digestive process. Indeed, we used millifluidic cells designed to bloc MFG and injected successively each lipase involved in the digestion process. Thank to the UV-microscope developed on the DISCO beamline, the autofluorescence of tryptophan (and tyrosine) present in the lipase amino-acid sequences had allowed the protein observation without external labeling. The gastric and intestinal neonatal disintegration of milk fat globules has been approached through transmission images recording and analysis of the number of globules and their state of aggregation/coalescence during digestion (approached via diameter, size, mode and specific surface evolution against time). In this presentation, the first results of these experiments will be showed. Mainly, in the stomach, the gastric lipases bind the MFG surface. Then, the MFG decreased in size in the intestinal phase releasing the intact lipid membrane suggesting that the core is hydrolyzed through the external membrane. [1] C. Bourlieu, S. Bouhallab, and C. Lopez. Biocatalyzed modifications of milk lipids: applications and potentialities, Trends in Food Science & Technology, 20 (2009) 458-469. [2] C. Bourlieu, and M-C. Michalski. Structure–function relationship of the milk fat globule. Current Opinion in Clinical Nutrition and Metabolic Care 18[2], 118-127. 2015.
Lipase activity on milk fat globules in a mini-digester to
simulate neonatal gastro-intestinal digestion using
DISCO beamline
Claire Bourlieu-Lacanal, Amélie Deglaire, Olivia Ménard, Thomas
Croguennec, Stéphane Pezennec, Juliane Floury, Steven Le Feunteun, Pierre
Villeneuve, Frédéric Carrière, Didier Dupont, et al.
To cite this version:
Claire Bourlieu-Lacanal, Amélie Deglaire, Olivia Ménard, Thomas Croguennec, Stéphane Pezennec,
et al.. Lipase activity on milk fat globules in a mini-digester to simulate neonatal gastro-intestinal
digestion using DISCO beamline. 14. GERLI Lipidomics meeting, Sep 2018, Saint Maximin la Sainte
Beaumes, France. hal-02356667
HAL Id: hal-02356667
https://hal.science/hal-02356667
Submitted on 2 Jun 2020
HAL is a multi-disciplinary open access
archive for the deposit and dissemination of scientific research documents, whether they are published or not. The documents may come from
teaching and research institutions in France or
abroad, or from public or private research centers.
L’archive ouverte pluridisciplinaire HAL, est
destinée au dépôt et à la diffusion de documents
scientifiques de niveau recherche, publiés ou non,
émanant des établissements d’enseignement et de
recherche français ou étrangers, des laboratoires
publics ou privés.
Lipase activity on milk fat globules in a mini-digester to simulate
neonatal gastro-intestinal digestion using DISCO beamline
Bourlieu C. 1,3*, Deglaire A. 1, Ménard O. 1, Croguennec T.1, Pezennec S.1, Floury J.1,
Le Feunteun S. 2, Villeneuve P.3, Carrière F.4, Dupont D.1, Bouhallab S.1, Fattaciolli
J.5, Jamme F.6, Vié V7
1
INRA-Agrocampus Ouest UMR 1253 STLO, France;
UMR 782 INRA-AgroParisTech GMPA, France;
3
CIRAD/INRA, UMR 1208 IATE, France;
4
CNRS, Aix Marseille University, UMR 7282, France;
5
ENS UMPC UMR CNRS 8640, France ;
6
Synchrotron SOLEIL, France;
7
IPR Institute of Physics, Rennes University 1, France;
*claire.bourlieu-lacanal@inra.fr
2
In their native state, milk lipids are present in the form of dispersed droplets called
Milk Fat Globules (MFG, diameter 0.1-20 μm, average = 4 μm in bovine milk) [1,2].
The native MFG is constituted by a triglyceride core covered with an external
trilayered membrane inherited from its secretory past. This membrane is mainly
based on polar lipids (glycerophospholipids, sphingolipids and glycosphingolipids),
proteins (25 % of total membrane with high proportion of glycoproteins and
enzymes), neutral lipids and minor components. The major biological function of the
MFG is to deliver energy to the mammal newborn, for that, the MFG has to be
hydrolyzed in the gastro-intestinal tract by lipases, in successive steps. The lipases
should interact with the MFG membrane and diffuse in the supramolecular object.
The aim of this work is to determine the mechanism involved in the enzymatic
hydrolysis along the digestive process.
Indeed, we used millifluidic cells designed to bloc MFG and injected successively
each lipase involved in the digestion process. Thank to the UV-microscope
developed on the DISCO beamline, the autofluorescence of tryptophan (and
tyrosine) present in the lipase amino-acid sequences had allowed the protein
observation without external labeling.
The gastric and intestinal neonatal disintegration of milk fat globules has been
approached through transmission images recording and analysis of the number of
globules and their state of aggregation/coalescence during digestion (approached via
diameter, size, mode and specific surface evolution against time).
In this presentation, the first results of these experiments will be showed. Mainly, in
the stomach, the gastric lipases bind the MFG surface. Then, the MFG decreased in
size in the intestinal phase releasing the intact lipid membrane suggesting that the
core is hydrolyzed through the external membrane.
[1] C. Bourlieu, S. Bouhallab, and C. Lopez. Biocatalyzed modifications of milk lipids: applications and potentialities,
Trends in Food Science & Technology, 20 (2009) 458-469.
[2] C. Bourlieu, and M-C. Michalski. Structure–function relationship of the milk fat globule. Current Opinion in Clinical
Nutrition and Metabolic Care 18[2], 118-127. 2015.
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La lógica nietzscheana es una psicología, pero una psicología de lo inconsciente y, por tanto, del alma, que Jung entendió como psique objetiva. El elemento esencial (hablamos de esencias, ya que hablamos del alma) de esta lógica nietzscheana es la tendencia hacia la unidad, tal tendencia además se remite, nos remite, a lo infinito. Se trata de la psicología de Dioniso. No podemos estudiar a Dioniso (sea éste lo que sea) para estudiar el alma, porque no lo conocemos lo suficiente, pero sí podemos estudiar la personalidad-alma de Nietzsche, es decir, la lógica que se expresa en, pero más allá de, sus palabras y textos. He llamado a esa lógica “lógica nietzscheana”. La lógica nietzscheana es el esquema básico que subyace en el proyecto de Nietzsche y que explica la totalidad de su obra y su finalidad.
Si este libro le ha interesado y desea que lo mantengamos informado de nuestras publicaciones, puede escribirnos a comunicacion@editorialsirio.com o bien regristrase en nuestra página web: www.editorialsirio.com Título original: THE OBESITY CODE Traducido del inglés por Francesc Prims Diseño de portada: Nayeli Jiménez Composición ePub por Editorial Sirio S.A.
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