ARTHROPOD BIOLOGY
Daily and Seasonal Patterns in Abdominal Color in Diaphorina citri
(Hemiptera: Psyllidae)
ERIK J. WENNINGER1
AND
DAVID G. HALL
USDAÐARS, U.S. Horticultural Research Laboratory, 2001 South Rock Road, Fort Pierce, FL 34945
Ann. Entomol. Soc. Am. 101(3): 585Ð592 (2008)
ABSTRACT Diaphorina citri Kuwayama, a psyllid vector of huanglongbing (citrus greening disease),
exhibits three more or less distinct abdominal colors in the adult psyllid: gray/brown, blue/green, and
orange/yellow. We explored the daily (in individuals in the laboratory) and seasonal (in a Þeld
population) patterns in abdominal color of adult D. citri to clarify the biology of this species in relation
to abdominal color and investigated the relationship between abdominal color and the reproductive
state of adults (i.e., whether an individual is reproductively mature, has mated, orÑin femalesÑis
gravid). Females were predominantly blue/green throughout their lives, with a small portion of
individuals being gray/brown, especially just after emergence. Approximately 86% of mated females
developed an orange/yellow abdominal color after mating, but they ultimately turned back to
blue/green within several days to 1 mo after mating. Only 31% of virgin females turned orange/yellow.
Males were predominantly blue/green early in life, but a greater portion of males relative to females
were gray/brown. The orange/yellow color in females reßected the presence of eggs in the abdomen;
in males it seemed to derive from the color of the internal reproductive organs, and it was generally
only evident in older males. The preponderance of blue/green females, rarity of gray/brown females
relative to gray/brown males, and rarity of orange/yellow males and females was largely reßected in
sticky card trap captures from the Þeld. Abdominal color is of essentially no value in discerning the
state of sexual maturity and of only limited value in discerning whether females have mated.
KEY WORDS Asian citrus psyllid, body color, pigment, reproductive state, huanglongbing
The psyllid Diaphorina citri Kuwayama (Hemiptera:
Psyllidae) vectors three phloem-restricted, noncultured bacteria in the genus “Candidatus Liberibacter,”
the causative agents of huanglongbing (citrus greening disease) (Halbert and Manjunath 2004, Hung et al.
2004, Bové 2006). First found in Florida in June 1998
(Tsai et al. 2000), D. citri has since spread throughout
the stateÕs citrus-growing regions (Michaud 2004). D.
citri may cause direct feeding damage (Michaud
2004), but the primary economic importance of the
psyllid is transmission of huanglongbing, one of the
worldÕs most serious diseases of citrus (Bové 2006).
Huanglongbing was Þrst found in southern Florida in
August 2005, and only “Candidatus Liberibacter asiaticus” is known to occur in the state (Bové 2006).
Citrus trees infected by this disease may live only 5 to
8 yr, during which time they produce misshapen,
poorly colored, bitter-tasting, unmarketable fruit
(Halbert and Manjunath 2004, Bové 2006). Despite
the great economic importance of D. citri as a vector
Mention of trade names or commercial products in this publication
is solely for the purpose of providing speciÞc information and does not
imply recommendation or endorsement.
1 Corresponding author, e-mail: erik.wenninger@ars.usda.gov.
of huanglongbing, relatively little is known about the
basic biology and ecology of this pest.
Variation in body color has been described in numerous species in the Sternorrhyncha, and the clariÞcation of the biological and ecological signiÞcance of
such color polymorphisms remains an active area of
research. Color variation in psyllids is generally associated with different seasonal forms in which the winter morphotype is darker, which presumably has a
thermoregulatory function. Examples include the pear
psylla, Cacopsylla pyricola (Förster) (Wong and Madsen 1967, Krysan and Higbee 1990); Cacopsylla midoriae (Miyatake) (Inoue 2004); Cacopsylla elegans
Inoue (Inoue 2004); Cacopsylla chinensis (Yang & Li)
(Yang et al. 2004); and Agonoscena pistaciae Burckhardt & Lauterer (Mehrnejad and Copland 2005).
Similar seasonal variation in body coloration also has
been described in many species in the Aphididae (e.g.,
Watt and Hales 1996; Stoetzel and Miller 1998, 2001;
Toros et al. 2003). Color polymorphisms in psyllids and
aphids have generally been described in populations
over time rather than at the level of individuals, and
individual body color may not be dynamic for many
species (but see Nevo and Coll 2001).
Confusion has surrounded the characterization of
different abdominal colors observed in D. citri, par-
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ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA
ticularly regarding the relationship between abdominal color and reproductive state. Inconsistent terminology to describe what is apparently the same color
category may contribute to the confusion. For example, Husain and Nath (1927) described the three categories of abdominal colors found in D. citri to be
“grayish brown,” “bluish,” and “orange,” whereas Skelley and Hoy (2004) used the terms “tan,” “green,” and
“orange.” Hoffman (1933) and Pande (1971) described the abdominal color of D. citri only as “dark
brown” and “brown-gray,” respectively. Tsai and Liu
(2000) did not refer to adult colors, but they stated
that the abdomens of mature nymphs turn either “bluish green” or “pale orange.”
Despite the variation in terminology, there seems to
be some agreement on the distinction of three broad
color categories in D. citri. Based on previous work
(Husain and Nath 1927, Tsai and Liu 2000, Skelley and
Hoy 2004) and on our own observations, we recognize
three more or less discrete color classiÞcations, each
of which includes a range of gradations between two
colors: gray/brown, blue/green, and orange/yellow.
Gray/brown individuals in particular show the most
variation in hue, in part because the cuticle of the
insect may be brown but covered to varying degrees
by white scales, yielding an overall gray appearance in
some individuals (Husain and Nath 1927). Additionally, color changes may take 1Ð2 d (E.J.W., unpublished data) and individuals undergoing a color
change often exhibit a mosaic of more than one colorÑmost notably when some blue/green females
change to orange/yellow as they produce eggs.
The change in abdominal color of adult D. citri to
yellow or orange has been thought to reßect reproductive state. For example, Husain and Nath (1927)
reported that the abdomen is usually orange in gravid
females, and more recently, Halbert and Manjunath
(2004) stated that the femaleÕs abdomen turns orange
when egg laying is imminent. Skelley and Hoy (2004)
equated reproductive maturity with a change in abdominal color from green to orange, stating that both
males and females reach reproductive maturity (i.e.,
turn orange) ⬇20 d after emerging as adults. We have
recently conÞrmed that adults of both sexes reach
reproductive maturity at 2Ð3 d after eclosion (Wenninger and Hall 2007)Ñwhich is more consistent with
reports of adults mating within hours to a few days
after emergence (Husain and Nath 1927, Pande
1971)Ñand we have observed that females with blue/
green abdomens are capable of laying fertile eggs
(E.J.W., unpublished data).
The different abdominal color morphs in D. citri are
not associated with other clear differences in morphology, and the abdominal color generally changes
over the life of an individual. Thus, the color polymorphisms seem not to represent seasonal morphs as
are found in many sternorrhyncan species in more
temperate climates (see above). Many insects exhibit
a green or blue/green body color due to a mixture of
yellow pigment (including carotenoids) and blue bile
pigments (Cromartie 1959, Law and Wells 1989, Saito
et al. 1998). The orange/yellow and blue/green col-
Vol. 101, no. 3
oration found in D. citri may be due to the presence
of carotenoids and blue bile pigments, which may be
present in the epidermal cells and/or the hemolymph
of insects (Barbier 1981, Law and Wells 1989, Saito et
al. 1998).
We performed laboratory studies with D. citri to
examine the lifetime dynamics in abdominal color in
mature nymphs and virgin and mated adults to clarify
any relationship between abdominal color and reproductive state. We also compared the mass of psyllids
of different sex and abdominal color. Additionally, we
sampled adult D. citri in citrus with yellow sticky cards
to examine the seasonal variation of the relative abundance of individuals in the Þeld having a given abdominal color. The goal of these studies was to clarify
the reproductive biology and ecology of D. citri in
relation to abdominal color.
Materials and Methods
Daily Patterns in Abdominal Color. All adult psyllids used in experiments were collected as Þfth instars
from a laboratory colony reared on Murraya paniculata (L.) Jack at the United States Horticultural Research Laboratory, as described by Hall et al. (2007).
We transferred Þfth instar nymphs individually to M.
paniculata seedlings (2Ð3-leaf stage) caged in plastic
vial containers, described in Wenninger and Hall
(2007). Brießy, each cage consisted of a 52-mm-tall
vial, modiÞed as an open-ended cylinder with a foam
plug used to stopper the top opening and two ventilation holes on the sides; individual cages were slipped
over a seedling grown in a cone-shaped planting container. Until ready for use in experiments, adult psyllids were held in an environmental chamber at 26⬚C,
60% RH, and a photoperiod of 14:10 (L:D) h, which
resulted in 70 Ð 80% RH inside the vials. Light intensity
(from ßuorescent lights) just above the plastic vial
containers was ⬇3,200 lux. Every 24 h until they died,
we examined each psyllid, recording the day that each
nymph eclosed as an adult and the abdominal color.
Each psyllid was assigned to one of the three more or
less distinct color categories (gray/brown, blue/
green, or orange/yellow) or to two intermediate categories (gray/brown to blue/green or blue/green to
orange/yellow) based on the color of the ventral side
of the abdomen.
All of the males observed (n ⫽ 41) and about half
(n ⫽ 70) of the 127 females observed were used in
mating studies in which males and females at 3Ð7 d
postemergence were paired for at least 24 h and no
more than 2 wk and isolated thereafter (Wenninger
and Hall 2008). The remaining females (n ⫽ 57) were
left unmated. To provide new oviposition sites, all
mated females (and 13 of the virgin females) were
transferred to a new seedling four times at 4 Ð5-d
intervals; thereafter, each female was maintained on
the same seedling for the duration of her life. The
remaining virgin females and all males (after mating)
were maintained on the same seedling for the duration
of their lives. All psyllids were held on individual
seedlings that were matched as far as possible for age
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WENNINGER AND HALL: ABDOMINAL COLOR PATTERNS IN D. citri
Table 1. Comparison of wet mass (mean ⴞ SEM) of colonycollected D. citri among sex and abdominal color combinations
Sex
Abdominal
color
n
Psyllid mass
(mg)
Female
Female
Female
Male
Male
Gray/brown
Blue/green
Orange/yellow
Gray/brown
Blue/green
42
34
34
37
22
0.343 ⫾ 0.013a
0.486 ⫾ 0.023c
0.545 ⫾ 0.020d
0.337 ⫾ 0.014a
0.410 ⫾ 0.022b
Means within a column followed by the same letter are not significantly different (␣ ⫽ 0.05; TukeyÕs pairwise comparisons test).
and development of ßush (immature leaves as described by Hall and Albrigo 2007); development of
plants was slow enough that ßush was constantly available to psyllids for the duration of the experiment
regardless of whether they were transferred to new
seedlings. After all psyllids died, we graphically examined patterns of daily changes in abdominal color of
the three groups of psyllids observed (virgin females,
mated females, and mated males).
Psyllid Mass in Relation to Abdominal Color. From
our laboratory colony, we collected D. citri adults
⬇7Ð10 d after eclosion and determined the sex and
abdominal color of each individual. We obtained the
following color and sex combinations: gray/brown,
blue/green, and orange/yellow females; and gray/
brown and blue/green males (see Table 1 for sample
sizes); we were unable to Þnd any orange/yellow
males on the collection date. Only psyllids that could
be readily assigned to a distinct color category were
used (i.e., no gray/brown to blue/green or blue/green
to orange/yellow psyllids were used). Immediately
after sexing psyllids, they were placed in a freezer and
held there overnight. We then measured the wet mass
of each psyllid to the nearest 0.01 mg. The data were
normally distributed (based on KolmogorovÐSmirnov
test and examination of residual plots), but variances
were not equal (LeveneÕs test); therefore, we used
weighted least squares analysis of variance (ANOVA)
to compare body mass among the Þve color and sex
combinations. Weights were calculated using the absolute value of each residual (兩 ei 兩) from ordinary least
squares ANOVA as an estimate of standard deviation
(i); each observation was then weighted by the reciprocal of the estimate of its variance (1/i2). TukeyÕs
pairwise comparisons test was used to discriminate
among means.
Seasonal Patterns in Abdominal Color. We deployed yellow sticky cards in citrus to sample adult
psyllids and examine the seasonal patterns in the relative abundance of individuals of different abdominal
color. The study was conducted in a block of ÔHamlinÕ
orange trees (Citrus sinensis L.; 4 yr old, ⬇2 m tall) in
a USDAÐARS grove near Fort Pierce, FL (Saint Lucie
County). No systemic or foliar insecticides were applied before or during the course of the study. Yellow
sticky traps (7.62 by 12.7 cm; sticky on both sides; a
bright yellow hue similar to S-G-390 by Behr Process
Corp., Santa Ana, CA) were obtained from Great
Lakes IPM (Vestaburg, MI). One trap per tree was
587
placed in each of 10 trees randomly selected across the
block of trees. Traps were suspended 1Ð1.5 m above
ground near the outside of the canopy from a branch
by using a black twist tie (178 mm length, MultiPurpose Tie/UV; Thomas and Betts Corp., Memphis,
TN). The traps were deployed in the Þeld for 1-wk
durations at biweekly intervals over 52 wk, beginning
on 4 January 2005. After collection from the Þeld, traps
were stored in a freezer in the laboratory. The number
of adult psyllids of each sex and color (only the three
distinct categories were used: gray/brown, blue/
green, or orange/yellow) combination per trap was
tabulated for each wk. Data were analyzed using a
separate 2 by 2 chi-square contingency table (Quinn
and Keough 2002) for each week (because so few
orange/yellow individuals were collected on sticky
cards, this color category was not included in the
analysis). For cases in which the expected count
within one or more cells was ⬍5, we used Fisher exact
test rather than chi-square.
Before collecting seasonal sticky card data, we performed a pilot study in which we placed psyllids of
known abdominal color on sticky cards, left them in
the Þeld for 1 wk, and then we transferred them to a
freezer for 24 h and determined whether individuals
retained their original abdominal color (E.J.W., unpublished data). Gray/brown individuals were easily
recognized, but many orange/yellow individuals that
initially had a more yellow appearance became clearly
orange (but still distinct from other color categories).
Many individuals that were initially blue/green were
predominantly gray/brown after freezing, with only
the anterior portion of the abdomen remaining distinctly blue/green; therefore, such individuals were
categorized as blue/green.
Results
Daily Patterns in Abdominal Color. Fig. 1 presents
results for observations on the daily color of adults
through 70 d after eclosion, but 12 of the 57 virgin
females observed and 10 of the 70 mated females
observed lived beyond 70 d (some for 100 d or longer),
staying blue/green for the remainder of their lives.
The two males that lived beyond 70 d were either
orange/yellow or gray/brown until death. On the day
preceding adult eclosion, ⬇64% of female nymphs and
70% of male nymphs were blue/green, with the remaining individuals exhibiting the “pale orange” coloration mentioned by Tsai and Liu (2000) (Fig. 1).
After adult eclosion, the portion of female psyllids
with a gray/brown abdomen gradually diminished and
stabilized to ⬇5Ð10%, reßecting for the most part a
small number of females that remained gray/brown
until death (Fig. 1A and B). Beginning at 5 d after
eclosion, the portion of virgin females with an orange/
yellow or blue/green to orange/yellow abdomen increased and ultimately ßuctuated ⬇3Ð 8%; ⬇33% of
virgin females had some degree of orange/yellow coloration at some point in their lives, but few individuals
exhibited the color at any given age (Fig. 1A). All
virgin females were blue/green by 60 d after eclosion
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ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA
Vol. 101, no. 3
Fig. 1. Daily changes in abdominal color and adult survival for virgin female (A), mated female (B), and mated male (C)
D. citri. Initial sample sizes were 57, 70, and 41, respectively. Age “0 d” represents the day of adult eclosion. Mated psyllids
were paired for between 24 h to 2 wk beginning at 3Ð7 d after eclosion. Bar color corresponds to abdominal color as follows:
brown, gray/brown; stippled brown, gray/brown to blue/green; green, blue/green; stippled green, blue/green to orange/
yellow; yellow, orange/yellow. For simplicity, nymphs (age ⫺3, ⫺2, ⫺1 d) that were pale orange were categorized in the
gray/brown group.
and through the remainder of their lives. For mated
females, the portion of individuals with an orange/
yellow or blue/green to orange/yellow abdomen increased sharply at 6 Ð7 d after eclosion and peaked
⬇40 Ð 60% ⬇12Ð21 d after eclosion (Fig. 1B). Across
days 6 Ð37 after emergence, 32.5 ⫾ 1.9% (mean ⫾
SEM) of mated females were orange/yellow or blue/
green to orange/yellow (Fig. 1B). A greater percentage of mated females (86%) relative to virgins exhibited an orange/yellow abdominal hue at some point in
their lives, but by 49 d after eclosion and for the
remainder of their lives, the abdominal color of all
mated females was blue/green. Of the females that
turned orange/yellow or blue/green to orange/yellow, the mean ⫾ SEM age at which the abdomen
changed color was 9.0 ⫾ 0.5 d after eclosion (4.0 ⫾
0.5 d after mating) for mated females (n ⫽ 60) and
10.7 ⫾ 1.7 d after eclosion for virgin females (n ⫽ 19).
Males showed more variation in abdominal color
(Fig. 1C). Compared with females, a greater portion
of males were gray/brown or gray/brown to blue/
green, and they tended to remain so for longer periods.
Males of orange/yellow abdominal color were observed at a very low frequency until around 40 d after
eclosion when the orange/yellow color category gradually became the dominant color observed. Of the few
males that lived beyond 57 d, none exhibited any
blue/green coloration. We did not observe an obvious
change in abdominal color of males that could be
linked with the timing of mating (age 3Ð7 d). Neither
May 2008
WENNINGER AND HALL: ABDOMINAL COLOR PATTERNS IN D. citri
589
Fig. 2. Mean number of adult female D. citri per yellow sticky card per wk, separated by abdominal color. Traps were
deployed in the Þeld for 1-wk durations at biweekly intervals beginning on 4 January 2005. Asterisks indicate wk in which
the observed total frequency of blue/green and gray/brown females was greater than or less than, respectively, expected
frequencies based on chi-square tests on contingency table data (see Table 2). Analyses were not performed for 7 November,
21 November, or 5 December in which three cells contained frequencies of zero.
males nor females showed an obvious change in abdominal color that corresponded with the onset of
reproductive maturity (2Ð3 d after emergence).
Female lifespan was reduced in the mating treatment that featured multiple males housed with individual females for 2 wk (Wenninger and Hall 2008).
With this treatment excluded, female longevity did
not differ between virgin (mean ⫾ SEM, 45.1 ⫾ 4.8)
and mated (42.8 ⫾ 4.3) females (t ⫽ ⫺0.36, df ⫽ 113,
P ⫽ 0.721; t-test). However, male longevity (28.2 ⫾
2.5 d) was signiÞcantly lower than that of overall
female longevity (43.9 ⫾ 3.2) (t ⫽ 2.8, df ⫽ 154, P ⫽
0.005; t-test).
Psyllid Mass in Relation to Abdominal Color. Psyllids of different color and sex combination differed
signiÞcantly in body mass (F ⫽ 1,055.2; df ⫽ 4, 163; P ⬍
0.0001) (Table 1). Gray/brown females and males did
not differ signiÞcantly from each other but had lower
mass than the other three groups, which each differed
signiÞcantly among each other. The mass of gray/
brown females was ⬇30% lower than that of blue/
green females, and for gray/brown versus blue/green
males the difference was ⬇18%. Orange/yellow females had the greatest mass, followed by blue/green
females and blue/green males; the mass of blue/
green females was ⬇11% lower than that of orange/
yellow females.
Seasonal Patterns in Abdominal Color. The total
abundance of males and females collected on yellow
sticky cards was remarkably similar over the course of
the yr (Figs. 2 and 3). Blue/green individuals (both
male and female) were generally more abundant than
gray/brown individuals; however, based on chi-square
tests on contingency table data (Table 2), there were
typically more blue/green relative to gray/brown females than expected and fewer blue/green relative to
gray/brown males than expected. In fact, for each
week in which the observed and expected frequencies
differed signiÞcantly, blue/green females and gray/
brown males occurred in frequencies that were
greater than expected by chance. Orange/yellow
individualsÑnotably malesÑwere rarely found on
sticky cards (Fig. 3), but orange/yellow females were
more abundant than at any time of the year 2 wk after
Fig. 3. Mean number of adult male D. citri per yellow sticky card per week, separated by abdominal color. Additional
notes as in Fig. 2.
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ANNALS OF THE ENTOMOLOGICAL SOCIETY OF AMERICA
Vol. 101, no. 3
Table 2. Two-way contingency tables showing the total and expected (in parentheses) counts of male and female D. citri with
gray/brown and blue/green abdominal color collected across 10 yellow sticky cards on each sample date in 2005
4 Jan. (week 1)
Gray/
brown
Female
Male
Blue/
green
2 (7.8)
83 (77.2)
12 (6.2)
56 (61.8)
2 ⫽ 10.6, P ⫽ 0.001
28 Feb. (week 9)
Female
Male
Female
Male
0 (1.0)
7 (6.0)
2 (1.0)
5 (6.0)
P ⫽ 0.462
31 Jan. (week 5)
Blue/
green
1 (2.6)
24 (22.4)
4 (2.4)
19 (20.6)
2 ⫽ 2.3, P ⫽ 0.129
Gray/
brown
Blue/
green
1 (6.4)
26 (20.6)
11 (5.6)
13 (18.4)
2 ⫽ 12.5, P ⬍ 0.001
14 Feb. (week 7)
Gray/
brown
Blue/
green
0 (6.8)
40 (33.2)
20 (13.2)
58 (64.8)
2 ⫽ 12.4, P ⬍ 0.001
14 Mar. (week 11)
29 Mar. (week 13)
11 April (week 15)
0 (1.4)
2 (0.6)
9 (7.6)
2 (3.4)
P ⫽ 0.077
5 (20.7)
73 (57.3)
47 (31.3)
71 (86.7)
2
⫽ 26.9, P ⬍ 0.001
1 (4.5)
51 (47.5)
9 (5.5)
54 (57.5)
P ⫽ 0.022
25 April (week 17)
9 May (week 19)
23 May (week 21)
6 June (week 23)
9 (18.2)
129 (119.8)
27 (17.8)
108 (117.2)
2
⫽ 10.8, P ⫽ 0.001
1 (11.7)
99 (88.3)
22 (11.3)
75 (85.7)
2
⫽ 22.4, P ⬍ 0.001
27 (43.4)
49 (32.6)
81 (64.6)
32 (48.4)
2
⫽ 24.3, P ⬍ 0.001
7 (13.9)
48 (41.1)
26 (19.4)
50 (56.9)
2
⫽ 7.8, P ⫽ 0.005
20 June (week 25)
Female
Male
3 (2.6)
5 (5.4)
Female
Male
0 (1.9)
7 (5.1)
7 (7.4)
16 (15.6)
P ⫽ 1.00
15 Aug. (week 33)
3 (1.1)
1 (2.9)
P ⫽ 0.024
11 Oct. (week 41)
Female
Male
18 Jan. (week 3)
Gray/
brown
0 (0.6)
1 (0.4)
4 (3.4)
2 (2.6)
P ⫽ 0.429
5 July (week 27)
18 July (week 29)
1 Aug. (week 31)
0 (4.2)
10 (5.8)
10 (5.8)
4 (8.2)
P ⬍ 0.001
10 (9.8)
7 (7.2)
13 (13.2)
10 (9.8)
2 ⫽ 0.02, P ⫽ 0.884
2 (8.0)
11 (5.0)
22 (16.0)
4 (10.0)
2 ⫽ 17.6, P ⬍ 0.001
29 Aug. (week 35)
12 Sept. (week 37)
2 (5.0)
8 (5.0)
15 (12.0)
9 (12.0)
2 ⫽ 5.1, P ⫽ 0.024
0 (0.8)
5 (4.2)
1 (0.2)
0 (0.8)
P ⫽ 0.167
26 Oct. (week 43)
(week 45, 47, 49)a
0 (0.7)
1 (0.3)
2 (1.3)
0 (0.7)
P ⫽ 0.333
(zero frequencies in
three cells)
26 Sept. (week 39)
0 (2.0)
4 (2.0)
7 (5.0)
3 (5.0)
P ⫽ 0.07
19 Dec. (week 51)
1 (1.3)
2 (1.7)
3 (2.7)
3 (3.3)
P ⫽ 1.00
For cases in which the expected count within one or more cells was ⬍5, FisherÕs exact test rather than chi-square was used to determine
whether the observed frequencies differed signiÞcantly from expected frequencies. Sample dates indicate the date traps were deployed; traps
were retrieved 7 d later.
a
For week 45, 47, and 49, the data were not analyzed because three cells contained frequencies of zero.
the springtime peak total abundance of psyllids
(Fig. 2).
Discussion
Color polymorphisms in psyllids and aphids have
generally been described at the population level
rather than in individuals (Wong and Madsen 1967;
Krysan and Higbee 1990; Horton 1993; Watt and Hales
1996; Stoetzel and Miller 1998, 2001; Toros et al. 2003;
Inoue 2004; Yang et al. 2004). For many species, individual variation in body color may not be dynamic
(but see Nevo and Coll 2001). The variation in abdominal color that we describe in D. citri occurs at the
level of the individual and seems not to reßect seasonal
morphotypes, but rather the contents of the abdomen
(Husain and Nath 1927). Moreover, the color variants
are not associated with any other obvious differences
in morphology, as occurs in the pear psylla, for example (Wong and Madsen 1967, Krysan and Horton
1991). Although the relative abundance of different
color forms did vary over the season, psyllids from
each of the different color categories that we observed
may generally be found at some level over much of the
year. Because seasonal morphotypes are generally associated with summerforms and overwintering win-
terforms (see Introduction), seasonal morphotypes
might not be expected in a tropical to subtropical
psyllid species.
The results of our study show that male and female
D. citri differ notably in daily and seasonal patterns of
abdominal color. However, abdominal color is of essentially no value in discerning the state of sexual
maturity and of only limited value in discerning
whether females are mated. Females often, but not
always, exhibited a change in abdominal color to orange/yellow after mating, whereas males showed no
change in abdominal color that could be clearly linked
with mating (although we did not monitor unmated
males). Such a change in the abdominal color of females after mating is not surprising given that the
orange/yellow coloration is associated with the presence of eggs in the femaleÕs abdomen (Husain and
Nath 1927; Halbert and Manjunath 2004), especially
for females that are exhibiting high oviposition rates
(E.J.W., unpublished data). Because one third of virgin females exhibited a change in abdominal color to
orange/yellow, the use of abdominal color as an indicator of reproductive state in female D. citri can be
implemented only with caution. Skelley and Hoy
(2004) stated that both male and female D. citri exhibit
a change in abdominal color (to orange) at ⬇20 d after
May 2008
WENNINGER AND HALL: ABDOMINAL COLOR PATTERNS IN D. citri
adult emergence, reßecting the onset of reproductive
maturity. Not only did we not observe such a change
in our observations of daily color dynamics, but we
also have shown that males and females reach reproductive maturity at 2Ð3 d after eclosion (Wenninger
and Hall 2007).
The abdominal color of most individuals of both
sexes is at least partly blue/green for much of their
lives, with a small portion of individuals exhibiting a
gray/brown abdominal color on each day, especially
early in life; however, gray/brown abdominal color is
more frequently observed in males than in females.
We speculate that a change in abdominal color from
gray/brown to blue/green is associated with feeding
activity and might be related to the quantity of food
ingested or whether psyllids are feeding on ßush or
mature leaves. The patterns that we observed in psyllid mass in relation to sex and color combinations are
consistent with a relationship between feeding and
abdominal color; within each sex, blue/green individuals were heavier than psyllids that were gray/brown.
Attempts to test whether a change in abdominal color
to gray/brown can be induced by starvation have been
largely unsuccessful (E.J.W., unpublished data), because D. citri does not survive long off of a host plant
(McFarland and Hoy 2001).
The relationship of abdominal color at mating with
reproductive output in D. citri remains to be thoroughly investigated, but we have found that gray/
brown females lay signiÞcantly fewer eggs than blue/
green females in the initial days after mating, although
total fecundity may not differ (E.J.W., unpublished
data). Interestingly, a small portion of females in the
current study maintained a gray/brown abdominal
color for as long as 35Ð36 d (Fig. 1A), but the abdominal color of mated gray/brown females ultimately
usually changes to blue/green or orange/yellow (Fig.
1B; E.J.W., unpublished data). It remains unclear why
some females maintain a gray/brown abdominal color
for extended periods.
The relative abundance of psyllids of different abdominal color that we observed in the laboratory was
largely reßected in the sex and color combinations
found on sticky cards. For both sexes, psyllids of blue/
green abdominal color were generally the most abundant, consistent with the generally large frequency of
this color category for psyllids maintained in the laboratory. Moreover, the greater than expected frequency of blue/green females and gray/brown males
collected on sticky cards was consistent with the
greater abundance of gray/brown males relative to
gray/brown females in the laboratory-maintained
psyllids. However, during several wk the abundance of
males that were gray/brown relative to those that
were blue/green was greater than would be expected
based on the laboratory data in which, among psyllids
under 30 d of age, only ⬇10 Ð20% were gray/brown.
Assuming the relative abundance of gray/brown
males in our laboratory data are representative of the
Þeld population, a greater than expected capture rate
of gray/brown males suggests that these males were
more active dispersers. Whether a high relative abun-
591
dance of gray/brown males on sticky cards reßects
greater dispersal or a greater relative abundance of
this color category in the Þeld remains to be examined.
Psyllids of orange/yellow abdominal colorÑ especially malesÑwere rarely found on sticky cards. This
pattern likely reßects the relative rarity of this color
form in both sexes. Moreover, males in the lab exhibited orange/yellow coloration generally only after
reaching an above-average age; in the Þeld, male survival beyond 30 Ð 40 d might be even rarer. The scarcity of orange/yellow females on sticky cards also
might reßect more limited dispersal tendencies in
gravid females. Fully gravid females in the Þeld and in
laboratory colonies are more likely to walk than ßy
away when physically disturbed (Skelley and Hoy
2004; E.J.W., unpublished data), possibly because they
are too heavy for sustained ßight or too driven to
oviposit.
The goal of these studies was to develop a more
thorough understanding of the biology, ecology, and
behavior of D. citri that may facilitate more effective
monitoring and management strategies. Our data suggest that the abdominal color of D. citri cannot be used
as a reliable indicator of reproductive state for females.
Several matters regarding the dynamics in abdominal
color in D. citri require further investigation, including
the underlying reasons for changes in abdominal color
(particularly to and from gray/brown and blue/
green), whether tendencies toward certain colors are
heritable, and to what extent abdominal color relates
to reproductive output in females.
Acknowledgments
For technical assistance, we thank the following individuals: Kathy Moulton, Matt Hentz, Chris Knox, Lynn
Faulkner, and Carrie Kahr (USDAÐARS, U.S. Horticultural
Research Laboratory, Fort Pierce, FL). For helpful comments on an earlier draft of the manuscript, we are grateful
to David Horton (USDAÐARS, Yakima Agricultural Research
Laboratory, Wapato, WA) and Michael Rogers (University of
Florida).
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Received 12 September 2007; accepted 4 February 2008.