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Biotechnology Advances
Towards high-yield production of pharmaceutical proteins with plant cell suspension cultures2011 •
2003 •
Plants provide an inexpensive and convenient system for the large-scale production of valuable recombinant proteins. This principle has been demonstrated by the commercial success of several first-generation products, and many others are currently under development. Over the ...
The demand for recombinant proteins in terms of quality, quantity, and diversity is increasing steadily, which is attracting global attention for the development of new recombinant protein production technologies and the engineering of conventional established expression systems based on bacteria or mammalian cell cultures. Since the advancements of plant genetic engineering in the 1980s, plants have been used for the production of economically valuable, biologically active non-native proteins or biopharmaceuticals, the concept termed as plant molecular farming (PMF). PMF is considered as a cost-effective technology that has grown and advanced tremendously over the past two decades. The development and improvement of the transient expression system has significantly reduced the protein production timeline and greatly improved the protein yield in plants. The major factors that drive the plant-based platform towards potential competitors for the conventional expression system are cos...
Transgenic plants can be used for molecular farming for the production of recombinant pharmaceutical or industrial compounds. They offer attractive alternatives to produce low-cost recombinant pharmaceuticals or industrially-important proteins on a large scale. The feasibility of precise plant genetic manipulation, high-scale expression of recombinant proteins, rapid and easy scaling up, and convenient storage of raw material and less concern of contamination with human or animal pathogens during downstream processing has attracted biotechnologists to plastid and chloroplast engineering. Crop plants produce large amounts of biomass at low cost and require limited facilities. Molecular farming represents a novel source of molecular medicines, such as plasma proteins, enzymes, growth factors, vaccines and recombinant antibodies, whose medical applications are understood at the molecular level. Edible organs can be consumed as uncooked, unprocessed or partially-processed material, making them ideal for the production of recombinant subunit vaccines, nutraceuticals and antibodies designed for topical application. Based on this prominence, the present chapter is aimed to cover recent advancement for the production of plant pharmaceuticals.
Applied Microbiology and Biotechnology
Bench to batch: advances in plant cell culture for producing useful products2010 •
Despite significant efforts over nearly 30 years, only a few products produced by in vitro plant cultures have been commercialized. Some new advances in culture methods and metabolic biochemistry have improved the useful potential of plant cell cultures. This review will provide references to recent relevant reviews along with a critical analysis of the latest improvements in plant cell culture, co-cultures, and disposable reactors for production of small secondary product molecules, transgenic proteins, and other products. Some case studies for specific products or production systems are used to illustrate principles.
Plant Biotechnology Journal
Recent advances towards development and commercialization of plant cell culture processes for the synthesis of biomolecules2011 •
Biotechnology Advances
Role of genetic factors and environmental conditions in recombinant protein production for molecular farming2009 •
Reference Series in Phytochemistry
Platforms for Plant-Based Protein ProductionPlants have provided humans with useful molecules for many centuries, but only in the past 20 years has it become possible to use plants for the production of specific heterologous proteins. “Molecular farming” is an application of genetic engineering technology; it involves the use of plants, and potentially also animals, as the means to produce compounds of therapeutic value. Since the first recombinant plant-derived pharmaceutical protein (PDP), i.e., human serum albumin, was produced in transgenic tobacco and potato plants in 1990, molecular farming in plants has emerged as a new subject of sciences. The plant system has the potential to provide virtually unlimited quantities of these bio-products at low costs, which is impossible for any other systems. Plants have been vigorously tested for the production of numerous bio-products including vaccines, therapeutic proteins, industrial enzymes and proteins with other functions. The urgent demand from the rapidly growing market has also facilitated research & development in the downstream processing of plant-derived recombinant proteins. Various breakthroughs resulting from basic to practical research have led to the rapid advance of this subject. This report will analyse the technique of molecular farming, potential applications as well as the benefits and risks involved.
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