Friends are best man’s dog

Time management is a set of principles, practices, skills, tools, and systems working together to help you get more value out of your time with the aim of improving the quality of your life. The important point is that time management is not necessarily about getting lots of stuff done, because much more important than that is making sure that you are working on the right things, the things that truly need to be done. Smart time managers know that there is much more to do than anyone could possibly accomplish. So instead of trying to do it all, smart time managers are very picky about how they spend their time.

Segunda Vuelta, 2021

“La tensión entre sector propietario rural y Estado es un rasgo constitutivo de nuestra política”, entrevista con Tomás Allan y Ramiro Albina, 14/3/2021, en Segunda Vuelta. Disponible en: https://segundavueltarevista.com/2021/03/14/entrevista-a-roy-hora/

Journal of Neolithic Archaeology, 2023

In the years 2022 and 2023, the Trypillia hilltop settlement of Trinca-La Șanț in northern Moldova was investigated and excavated by the CRC1266 in collaboration with the State University of Moldova. As a result, we unveiled the basic structures of the 25 ha fortified settlement, which, adapted to the topography, combines the principles of linear and concentric rows of houses. Based on the 14 C data available to date, it can be assumed that the settlement dates from 3950 to 3650 BCE. Of the 320 houses discovered, up to 100 existed simultaneously, which corresponds to a maximum number of inhabitants of 250-1000 people. δ 13 C/δ 15 N isotope values of domestic animals indicate an extensive economy that corresponds to that of other, similarly large or mega-sites. Research questions As part of the CRC1266 investigations into transformation processes, we selected the northern Moldovan site of Trinca-La Șanț to investigate specific changes within the late Trypillian society. Several reasons prompted this decision, all of which tie back to three aspects.

Hepatoma Research, 2024

Aim: Hepatocellular carcinoma (HCC) in patients with Metabolic dysfunction-associated steatotic liver disease (MASLD, formerly NAFLD) is expected to be a significant public health issue in the near future. Therefore, understanding the tumor microenvironment interactions in MASLD-induced HCC is crucial, and the development of relevant preclinical models is needed. Hence, we aimed to determine the effects of a MASLD-mimicking microenvironment (ME) on the aggressiveness of HCC cells and identify target genes that drive HCC by developing a 3D-in vitro co-culture system. Methods: A 3D co-culture system mimicking the MASLD-ME was created with LX-2 liver stellate cells embedded in 3D collagen gel in the lower and SNU-449 HCC cells on the upper parts of Boyden chambers, and cells were grown in an optimized metabolic medium (MM). The effects of MASLD-ME on motility, sphere formation, proliferation, and cell cycle of SNU-449 cells were tested by Boyden chamber, 3D sphere formation, XTT, and Flow cytometry, respectively. The protein expression/activation profiles of motile SNU-449 cells that passed the membrane toward MASLD-ME or control condition were investigated using a multiplex protein profiling system DigiWest and confirmed with RT-PCR, WB, and Flow cytometry. IDH2 levels were examined in primary human HCC and adjacent liver tissues by IHC and in TCGA and CPTAC cohorts by bioinformatics tools. Results: MM treatment increased fat accumulation, motility, and spheroid formation of both SNU-449 and LX-2 cells. MASLD-ME induced activation of LX2 cells, leading to the formation of bigger colonies with many intrusions compared to related controls. DigiWest analysis showed that metabolism-related proteins such as IDH2 were the most affected molecules in SNU-449 cells that migrated toward the MASLD-ME compared to those that migrated toward the control condition. Downregulation of IDH2 expression was confirmed in SNU-449 cells grown in MASLD-ME, in primary HCC tumor samples by IHC, and in HCC patient cohorts by bioinformatics analysis. Conclusion: This study reports the potential involvement of MASLD-ME in the downregulation of IDH2 expression and promoted motility and colonization capacity of HCC cells. The 3D MASLD model presented in this study may be useful in investigating the mechanistic roles of MASLD-ME in HCC.

Vibrant, 2021

In 2014, the Supreme Court of Canada issued a landmark decision in Tsilhqot'in Nation v. British Columbia. In this decision, the Supreme Court recognised Aboriginal title to a specific territory for the first time, along with Aboriginal rights to hunt, trap, and engage in other practices. While international human rights law relating to Indigenous peoples, notably the United Nations Declaration on the Rights of Indigenous Peoples, was not directly relied upon in this decision, the subsequent negotiations and outcome documents have gradually included the UN Declaration into the discussion, in conjunction with the political and legal shift towards recognition and acceptance of it in Canada. By exploring the political and legal struggles of the Tsilhqot'in, particularly after the 2014 decision, this paper considers a growing space for the UN Declaration in defining the declared Aboriginal rights and title.

Elizabeth Gilbert