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2004
The present investigation reveals the results of heat and cold shock expression of HSP-70 in different organs of Cirrhinus mrigala (Ham.). Fishes were exposed to heat and cold shock treatments and thermosensitive organs viz. liver, brain, kidney and gills were dissected out and tissues were analyzed for HSP70 quantification. At acclimatised temperature (260 C) the HSP – 70 level was highest in liver (10.35 ± 0.8704) and lowest in gills (1.96 ± 0.29). The highest HSP-70 levels were obtained in brain and liver, respectively during heat shock treatment at 370 C for 1 hr, 2hrs, 4 hrs, 22 hrs, 27 hrs, 44 hrs and 48 hrs whereas the lowest HSP-70 levels were obtained in kidney and gills, respectively. In cold shock treatment at 170 C for 18 hrs, 27 hrs and 48 hrs the highest HSP-70 levels were obtained in liver and brain, whereas the lowest levels were obtained in gills.
Aquaculture Research
The induction of Hsp70 synthesis by non-lethal heat shock confers thermotolerance and resistance to lethal ammonia stress in the common carp, Cyprinus carpio (Linn)Biochemical and Biophysical Research Communications
Cloning and characterization of the hsp70 multigene family from silver sea bream: Modulated gene expression between warm and cold temperature acclimation2005 •
European Journal of Histochemistry
Immunohistochemical localization of constitutive and inducible Heat Shock Protein 70 in carp (Cyprinus carpio) and trout (Oncorhynchus mykiss) exposed to transport stress2008 •
Biological Chemistry
Transient Increase in Chloride Cell Number and Heat Shock Protein Expression (hsp70) in Brown Trout (Salmo trutta fario) Exposed to Sudden Temperature Elevation2000 •
2013 •
SYNOPSIS. This paper reviews the generalized stress response in fish at the cellular and neuroendocrine levels. The focus of this review is to examine the possible relationships between the stress responses at these two levels in fish. It focuses primarily on the heat shock protein 70 (hsp70). Thus, the descriptions of the endocrine and the cellular stress responses are followed by a discussion of how hsps may be related to the stress hormones adrenaline and cortisol. Preliminary evidence shows that adrenaline causes an increase in hsp70 in primary cultures of rainbow trout hepatocytes. Cortisol does not directly affect hsp70 levels in fish tissues; however, in primary cultures of trout hepatocytes, cortisol decreased the stressor-induced increase in hsp 70. A wide range of abiotic and biological stressors have been shown to induce hsp induction in many types of fish cells, including cell lines, primary cell cultures, and in tissues from whole animals. Heat shock proteins has been i...
Bulletin of Environmental Contamination and Toxicology
Seasonal Effect on Heat Shock Proteins in Fish from Kuwait Bay2010 •
Heat shock proteins (HSP70) play a significant role in adaptation to temperature and have been proposed as an indicator of cellular stress. Since the water temperature in Kuwait’s marine area varies from 13 to 35°C from winter to summer, HSP70 could be a valuable tool in aquaculture in Kuwait. HSP70 levels were quantified by Western blotting in liver, muscle and gill tissues of two varieties of native fish species captured during the winter and summer months from both inside and outside the highly stressed Kuwait Bay area. The HSP70 levels did not differ statistically between fish captured from the two sampling areas. The most common response in both species was higher median levels of HSP70 in winter months. This inverse relation between HSP70 levels in the fish and the water temperature may be due to either genetic adaptation in the fish to the hot climatic conditions of the region or other stressors, such as changes in pollutant levels in the surrounding water.
2010 •
Environmental Toxicology and Chemistry
Purification of the 70-kDa heat-shock protein from catfish liver: Immunological comparison of the protein in different fish species and its potential use as a stress indicator1994 •
The heat-shock protein or stress-70 family was isolated from catfish liver. The homogeneity of the purified protein was analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Fish subjected to whole-body hyperthermia contained the constitutive and the heat-inducible stress-70 with approximate molecular weights of 70 and 68 kDa, respectively. The final purification product from livers of catfish raised under normal temperature was only the constitutive stress-70. Western blot analysis with rabbit antiserum prepared against purified catfish (Ictalurus punctatus) liver stress-70 showed that the antibody cross-reacted with liver, muscle, and gill tissue homogenates of fathead minnows (Pimephales promelas), red shiners (Cyprinella lutrensis), black bass (Micropterus salmoides), and bluegill (Lepomis macrochirus), with various intensities suggesting that stress-70s from different tissues of various fish species share common antigenic determinants of the protein. This substantiates that the antigen/antibody approach of stress-70 is useful as a stress indicator and, consequently, as a potential biomarker for water quality.
A Cultural History of Mathematics, eds. Archibald and Rowe
Mathematics and Worldviews: Competing Visions in the Age of Ideologies2024 •
Please do not quote. This version of the paper is a preprint, and in fact very different from the published chapter in Vol. 6 of A Cultural History of Mathematics, eds. Archibald and Rowe (Bloomsbury). Any quote or reference should be from/to that book.
«Vida Nueva» nº3318 (230526)18-19
Católicos de España, por primera vez: disminuyen al 44%.Logeion 12
Αἰσχύλος, ἀναγνώστης τοῦ Ὁμήρου. Ἀπὸ τὴν ὀλολυγὴ τῆς Εὐρύκλειας στὸ εὖχος τῆς Κλυταιμήστρας2022 •
2019 •
Teológia és hűség ISBN 978-963-380-255-7
A körtefa és a torony [Der Birnbaum und der Turm]2023 •
2011 •
CURRENT APPROACHES TO TELLS IN THE PREHISTORIC OLD WORLD
Re-discovering the Neolithic landscapes of western Thessaly, central Greece. In A. Blanco-Gonzalez & T.L. Kienlin (eds.) CURRENT APPROACHES TO TELLS IN THE PREHISTORIC OLD WORLDMusamus Fisheries and Marine Journal
Economic Prospect Of Fishing Egg Flying for Coastal Communities in Fak Fak District, West Papua Province2019 •
North American Journal of Engineering and Research
Balancing Agility and Operational Overhead: Monolith Decomposition Strategies for Microservices and Microapps with Event-Driven Architectures2021 •
2014 •
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Noida Sector, 15 Call Girls : ((9711106444)) 💞 Low rate call girl service2021 •
Hypoxia
Aryl hydrocarbon receptor acts as a tumor suppressor in a syngeneic MC38 colon carcinoma tumor model2019 •
Frontiers in Child and Adolescent Psychiatry
Piloting creative engagement strategies to explore themes of parenthood with fathers2024 •
1985 •
International Journal of Computer Network and Information Security (IJCNIS)
Self-healing AIS with Entropy Based SVM and Bayesian Aggregate Model for the Prediction and Isolation of Malicious Nodes Triggering DoS Attacks in VANET2023 •