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g p p r! '" ," Q = 3 0-cn 2E L 3 #.S: r Q = 9 9 g g egs s g 58 zQ =-. rr 3. Q 2 2 3 S Q R CT 2g 'CI $$ a Q 9 5 =2 'Sa i.2 ' Ps g .=r$ 0- $ n n Z E x, 3 s g -Q;09=. =n. Ps 5 5 e " DE RAHMADlNA. Aktivitas dan Kinetika Fratease PseudOmtfas cejmcia pada Susu asmrbsi. Dibimbing oleh HENDRA ADUUWANA dan TATlK KHUSNLATI. Susu merupakan media pertumbuhan jarkg sempuma bag^ mhoo&me. .erusakan susu pasteurisasi (skim dan beriemak) d.is&&kan oleh aktivitas prdease seudonwnas cejmcia- Penelitiao ini mmgukw aktivitar dan LrinctJB proP. cepcia ada susu pastarrisasi skim d m bdemak. Produksi protease P. cepocia dilakukan engan media susu s b dan disemipurifikasi dengan amonium d f h t dm etanol. Nilai ptunalisasi ~ a a gdiperoleh digunakan untuk rnertentukan nilai KM dan V-. Aktbhs iu'i dengan menambahkan protease 1% pada subdrat k e m u h dismpao pada suhu 4 OC &a IS&. HomogeniraSsusudanaktivitaspmrsaSdiamlisispadaharikc-O(5hari ~ u kadalutvarsa), m hari ke-5 (tepat kadalu\xarsa), hari ke- 10 (5 hari setelah @u\vacsa), dan hari ke- 15 (10 hari setelah Wun.arsa). Hasil semipurifikasi le@njUWQ. bah\ra amonium rulFat 60% memberikao aktivitaJ pmteaw krlinggi ~ 2 . 6 2 2 7 U n i t r n C ' m e o i t - ' dmgaonilai K,danV-lxmmrtol~adalahS.7443 M V - adalah 1.6401 c l ~ / d ' -menit-'. Aktivitas pmtease pada susu slcim dan &mak ti& rnenunjukkaa perubahan homogenitas jang nyata. Eksarqa alrtivitas dihasilkan pada susu skim dan berlemak bemmt-turut adalab 1.0401 Unit clan Unit. Had ~angdiperoleh menunjukkan b a h m susu berlemak lebih cepaf rusak ~&dio~sususkim. 3 j E 2 Q s -.g 0 s 2 VI 3 3 S ss 5 tjj. 3 ABSTRACT - RAHMADINA. The Protease Activity and Kinetic of Pseudomonas cepacia in Milk. Under the direction of HENDRA ADLJUWANA and TATlK HEJSNZATI. Milks is a pwfed media h r microoqgmkm's gro\\lth. The spoilage of pasteurized ilks ( s k i and \\.hole) is caused by profease activity of Psetldonwluzs cepcia. This search was to chacterize the protease activity and kinetics of P. wpcia in pas&wkd produced using skim milk mediullks (skim and whole). Protease of P. cepncia rd \\as semi purified using ammonium sulfate and ethml. The obtained of )hmalization\\as used to h d KMandVmrb value. The activity assay was done by added YO of protease to substmtes &en stored in temperature 4 "C for 15 days. Analysis cluding milk appearance and prdease activity were done in day- 0 (5 days befbre expire ae). jcb day (expire date), 10m day (5 days after expire da&) and 1jrn day (I0 days after date). The s t m i purification result s h o n d tha! ammonium sulfate 0% gives the prdease activity (2.6227 Unit m.L-'- minute-') with Kx( and V- are 5.7443 and pM d-' minute-', . respectively. The appearance (milks homogeaeily) & o l d em \\as no significant different in appearance bet\veen skim and whole milk. The ~i value in skim and d o f e milk \wre 1.OW1 Unit mL-' and 1.4399 Unit rnL-': @ctively. The spoilage of \x+ole milk is faster tkm skim milk, I, 0 +uned c - F z-