Objective: The present study was to investigate the phyllanthin present in Phyllanthus niruri which belongs to the family, Euphorbiaceae. The vital aim was to evaluate the antioxidant and to determine the anticandidal efficacies against... more
Objective: The present study was to investigate the phyllanthin present in Phyllanthus niruri which belongs to the family, Euphorbiaceae. The vital aim was to evaluate the antioxidant and to determine the anticandidal efficacies against Candida albicans. Methods: Phyllanthin, is one of the active lignan which was isolated from Phyllanthus niruri by silica gel column chromatography employing gradient elution with hexane–ethyl acetate solvent mixture. The eluted samples were further identified and estimated using HPTLC. Free radical scavenging activity of phyllanthin was examined using DPPH assay. Anticandidal activity was studied by analysing the growth inhibition and Minimum Fungicidal Concentration (MFC) rate. Results: The retention time of phyllanthin is 23.4 and its total run time was 45 min. Phyllanthin exhibited very high antioxidative property by its low IC50=7.5 µmol/mL. The isolated phyllanthin alters the growth of C. albicans and showed significant results in vitro. Among the various concentrations of phyllanthin, 250 µl/L concentration revealed the high rate of inhibition. Conclusion: The phyllanthin compound from the medicinal plant, Phyllanthus niruri confirmed as an effective antifungal agent to prevent the candidiasis and the secondary infections caused in various diseases.
Cassia fistula seeds have many therapeutic uses in traditional medicine practice. The present investigation was undertaken to demonstrate the anticandidal activity of the C. fistula seed extract at ultra-structural level through... more
Cassia fistula seeds have many therapeutic uses in traditional medicine practice. The present investigation was undertaken to demonstrate the anticandidal activity of the C. fistula seed extract at ultra-structural level through transmission electron microscope (TEM) and scanning electron microscope (SEM) observations. The effect of seed extract on the growth profile of the Candida albicans was examined via time-kill assays and in vivo efficacy of the extract was tested in an animal model. In addition, the anticandidal effect of seed extract was further evaluated by microscopic observations using SEM and TEM to determine any major alterations in the ultrastructure of C. albicans. The complete inhibition of C. albicans growth was shown by C. fistula seed extract at 6.25 mg/mL concentration. The time-kill assay suggested that C. fistula seed extract had completely inhibited the growth of C. albicans and also exhibited prolonged anti-yeast activity. The SEM and TEM observations carried out to distinguish the metamorphosis in the morphology of control and C. fistula seed extract-treated C. albicans cells revealed the notable effect on the outer cell wall and cytoplasmic content of the C. albicans and complete collapse of yeast cell exposed to seed extract at concentration 6.25 mg/mL at 36 h. The in vitro time-kill study performed using the leaf extract at 1/2, 1 or 2 times of the MIC significantly inhibited the yeast growth with a noticeable drop in optical density (OD) of yeast culture, thus confirming the fungicidal effect of the extract on C. albicans. In addition, in vivo antifungal activity studies on candidiasis in mice showed a 6-fold decrease in C. albicans in kidneys and blood samples in the groups of animals treated with the extract (2.5 g/kg body weight). The results suggested that the C. fistula seed extract possessed good anticandidal activity and is a potential candidate for the development of anticandidal agents.