1. In rainbow trout, 3HAA activity was comparable with those of terrestrial animals; 3HAA:PC activity ratio suggests ineffective conversion of tryptophan to niacin. 2. Inactivation as well as reactivation under different conditions was... more
1. In rainbow trout, 3HAA activity was comparable with those of terrestrial animals; 3HAA:PC activity ratio suggests ineffective conversion of tryptophan to niacin. 2. Inactivation as well as reactivation under different conditions was investigated. 3. Some characteristics of the enzyme extract were studied with the aim of optimizing assay in fish.
Pendimethalin is a widely used herbicide in agricultural field to control weeds in many areas of India. Chemicals like herbicides; pesticides etc. after releasing from various agricultural fields impair water quality directly or... more
Pendimethalin is a widely used herbicide in agricultural field to control weeds in many areas of India. Chemicals like herbicides; pesticides etc. after releasing from various agricultural fields impair water quality directly or indirectly causing severe damage to the aquatic ecosystem especially to fishes. In the present study, the toxic effect of pendimethalin was evaluated by measuring tissue protein levels in a commercially important fish, Channa punctata. Adult healthy fishes were used and divided into 5 experimental groups; first group was kept as control, second one as DMSO (solvent) exposed, while fishes of third, fourth and fifth groups were exposed to 10% (0.220 mg/L), 20% (0.440 mg/L) and 30% (0.660 mg/L) of 96 h LC50 value of pendimethalin respectively, for duration of 96 hours in a semi static system. The results revealed a significant decrease in liver, gonad and blood protein levels in the exposed fishes in a dose and time dependant manner when compared to control. The occurrence of decreased protein level in the observed tissues of the treated fishes is the indications of dysfunctional protein metabolism in response to the test chemical.
Fish gills of Thunnus albacares have been chosen as a unique source of glycosaminoglycans (GAGs) in this study. Chemical and physical characterization were carried out by ATR-FTIR spectroscopy, DEAE-Cellulose anion exchange... more
Fish gills of Thunnus albacares have been chosen as a unique source of glycosaminoglycans (GAGs) in this study. Chemical and physical characterization were carried out by ATR-FTIR spectroscopy, DEAE-Cellulose anion exchange chromatography, SAX-HPLC, and NMR techniques to determine the nature of GAG isolated. The results showed that the isolated GAGs (8.5mg/g yield) were mostly Chondroitin sulphate (CS) type. SAX HPLC chromatogram of isolated GAG and CS A standard showed comparable peaks A at 4.9, B at 7.8 and C at 9.99 min. FTIR spectra, IR (KBr) υ max in cm-1 presented characteristic peaks of-CONH vibration of amide group, coupling of CO stretching vibration, S=O stretching vibrations and-CO -S molecules confirmed the presence of CS type GAG in T. albacaresgills. 1 H NMR spectra in D2Oalso revealed the presence of CS moiety. All these results substantiate T. albacares gills as an excellent source of CS type GAG.
