IBA

Protein accumulation was studied during the in vitro rooting of axillary shoots of Syringa vulgaris L. on half-strength MS medium supplemented with 7.5 mg l-1 IBA (Indole-3-butyric acid) and on auxin free one (control). The aim of the study was to determine the most appropriate duration of auxin application by monitoring protein content as a suitable marker for the rooting of Syringa vulgaris in vitro. The levels of this biochemical parameter were monitored on 12th, 24th, 36th, 48th, 60th and the 72nd hour after the beginning of the experiment. Our results showed that the protein content in auxin-treated explants was generally higher than in the control, except on the 36th hour. The beginning of root formation was indicated by a sharp decrease in total protein accumulation on IBA containing medium. On the basis of the results the most appropriate time for a transfer of in vitro cultivated shoots from inductive onto expressive rooting medium is around the 36th hour after the beginning of the cultivation.

An experiment was carried out at the Department of Horticulture at Sher-e-Bangla Agricultural University, Dhaka, Bangladesh for the investigation of the performance of different concentration of Indole-3-Butyric Acid (IBA) for sprouting and rooting potential of Bougainvillea spectabilis stem cutting during the period from May 2013 to August 2013. Five treatments viz. T0: Control; T1: IBA in dust form; T2: IBA 500-ppm; T3: IBA 1000-ppm and T4: IBA 2000-ppm were exploited on the experiment with five replication. In case of T2, T3 and T4 cuttings were soaked for 24 hours on the respective solutions. From the experiment it was found that IBA play an important role for successful asexual propagation of bougainvillea through cutting. Better performance was found when cut stems were soaked for 24 hours before the establishment of stem cutting. But performance was inhibited by increasing concentrations of IBA in terms of all of the features on which data had taken. During the experimental period, data was taken on different attributes and T3 (1000-ppm) was performed as the best among the treatments which as followings: days to first rooting (4.0), days to first sprout bud initiation (5.3), number of sprout bud/cutting (3.6), number of leaves/cutting (35.2), length of sprout (15.0 cm), number of branches/cutting (4.7), number of root/cutting (64.2), number of sub root/cutting (25.8), longest root length (33.2 cm), longest root diameter (0.51 mm), rooting % (100%), survival % of rooted cuttings (100%). Though 100% rooting was also found in 500-ppm and 2000-pmm but only 1000-ppm was resulted 100% survival of rooted cuttings.

The designation of Important Bird Areas (IBAs), a prioritization scheme developed by BirdLife International, is based on four quantitative ornithological criteria: 1) globally threatened spe cies, 2) restricted-range species, 3) biome-restricted species, and/or (4) fl ocking species. A total of 21 areas in Guatemala fulfi ll international IBA criteria. All IBAs were delimited based on spatial habitat requirements of key species and cover about 48% of the country. Guatemalan IBAs are crucial for the conservation of bird species endemic to the northern Central American highlands. The Guatemalan IBA network covers habitat for eight globally threatened species and 25 restricted-range species. Two sites support >1% of the biogeographic population of waterbird species. Guatemalan IBAs are rather large, ranging from 44 to 21 000 km2, and include not only intact habitat (61% of the area of all IBAs), but also areas where efforts should be invested to restore habitat (38%). Long-term habitat conser vation in the half of the country is challenging. Guatemala’s human population is growing rapidly, increasing the pressure on natural habitat. All parts of the Guatemalan society are addressed by the designation of large IBAs, which is an opportunity to enhance conservation efforts among the private and communal sectors.

An experiment was carried out at the Department of Horticulture at Sher-e-Bangla Agricultural University, Dhaka, Bangladesh for the investigation of the performance of different concentration of Indole-3-Butyric Acid (IBA) for sprouting and rooting potential of <i>Bougainvillea spectabilis</i> tem cutting during the period from May 2013 to August 2013. Five treatments viz. T<sub>0</sub>: Control; T<sub>1</sub>: IBA in dust form; T<sub>2</sub>: IBA 500-ppm; T<sub>3</sub>: IBA 1000-ppm and T4: IBA 2000-ppm were exploited on the experiment with five replication. In case of T<sub>2</sub>, T<sub>3</sub> and T<sub>4</sub> cuttings were soaked for 24 hours on the respective solutions. From the experiment it was found that IBA play an important role for successful asexual propagation of bougainvillea through cutting. Better performance was found when cut stems were soaked for 24 hours before the ...

Abstract: Aloe vera has valuable medicinal properties and is commercially used inpharmaceutical, cosmetic and food industries. An efficient micro propagation method hasbeen developed in Aloe vera plants using the shoot tip explants cultured on MS ...

— Myrtle (Myrtus communis L.) is a small tree shrub of the family Myrtace, grown naturally of the Mediterranean area. Myrtle is very important as an antiseptic, anti-inflammatory and hypoglycemic agent. Turkey has great genetic resources for myrtle. Propagation of myrtle genotypes is significant issue. Plant tissue culture techniques offer fast and reliable micropropagation for many plant species. Different media content could be used for micropropagation in in vitro condition. The aim of the present study is to determinate of effects of different media on micropropagation and rooting in myrtle. For this purpose, Murashige and Skoog (MS), Rugini Olive Medium (OM) and Woody Plant Medium (WPM) media were used for micropropagation and rooting experiments. All media were supplemented with 1 mg l-1 BA for micropropagation, 1 mg l-1 IBA for rooting. The rate of micropropagation and plant length, rooting rate, numbers of root and root length were determined. Rooted with well-developed shoots transferred to plastic pots containing autoclaved peat and perlite (1:1, v/v). The potted plants were placed in a greenhouse. Acclimatized plants were compared after eight weeks. Means were separated by analysis of variance and the LSD test was performed to examine significant differences. Based on the result, the best medium was detected WPM on micropropagation rate (6.75 per plant), and then MS (4.20 per plant), OM (3.70 per plant). According to rooting data the highest rooting rate was calculated in WPM with 100%, rooting rate in OM and MS media was detected 70% and 50%, respectively.

This study was carried out to determine the suitable
time for preparation of cuttings, type of cuttings, and various
IBA concentrations to root semi-hardwood cuttings of Turkish
tea clone cultivars in Rize (Turkey), during 2010 and 2011. The
cuttings were  collected on 15 July and  1 August. After pretreating with 0, 2000, 4000 and 6000 ppm IBA, the cuttings with
full leaf and half leaf cuttings were rooted in perlite medium at
the unheated but mist propagated glasshouse. Semi- hardwood
cuttings were exposed to the rooting media for 60 days, and then,
they all were removed from media to determine the survival rate,
rooting rate, root number, root length, root diameter and root
quality. In 2010, the survival rates were between 66.7 – 91.7 %;
the rooting rates were between 38.3 – 85.0 %; the root numbers
were between 3.2 – 6.4 units; the root lengths were between 8.5 –
14.8 cm; the root diameters were between 0.68  – 1.27 mm and
the root qualities were between 1.91 – 3.52. In 2011, the survival
rates were between 65. – 91.7 %; the rooting rates were between
51.7 – 85.0 %; the root numbers were between 3.1  – 8.5 units;