The tree Pometia pinnata Forst. forma glabra (B1.) Jacobs is an ant-plant that provides lodging (leaf domatia) and food (extrafloral nectar, excretions of coccids) for ants. The leaf domatia are formed by two pairs of strongly modified... more
The tree Pometia pinnata Forst. forma glabra (B1.) Jacobs is an ant-plant that provides lodging (leaf domatia) and food (extrafloral nectar, excretions of coccids) for ants. The leaf domatia are formed by two pairs of strongly modified basal leaflets. In total, 63 ant species from 18 genera and 4 subfamilies were encountered in the domatia of the host in the study area (Ulu Gombak, Malaysia). We found that the ant/Pometia relationship has two stages: (i) an early 'myrmecophilic' phase of juvenile, domatia-free plants, and (ii) a 'myrmecophytic' phase of older plants in which ants nest in domatia. In the myrmecophilic stage, our short-term experiment revealed a significant greater loss of young leaflets when ants were excluded than on control plants to which ants had access; however, the area of remaining leaf was not different between treatment and control. Similar ambiguous effects were observed in the myrmecophytic stage: a snapshot census revealed no significant difference in the standing level of leaf herbivore damage between branches with and without nesting ants. However, our survey indicated that ant species differed in the protection provided to their host. Our data also suggest that presence of nesting ants substantially reduces damage to the leaf rachis.
Symbioses between plants and fungi, fungi and ants, and ants and plants all play important roles in ecosystems. Symbioses involving all three partners appear to be rare. Here, we describe a novel tripartite symbiosis in which ants and a... more
Symbioses between plants and fungi, fungi and ants, and ants and plants all play important roles in ecosystems. Symbioses involving all three partners appear to be rare. Here, we describe a novel tripartite symbiosis in which ants and a fungus inhabit domatia of an ant-plant, and ...
Retaliation against cheaters can prevent the breakdown of cooperation. Here we ask whether the ant–plant Cordia nodosa is able to apply retaliatory sanctions against its ant symbiont Allomerus octoarticulatus, which patrols new shoots to... more
Retaliation against cheaters can prevent the breakdown of cooperation. Here we ask whether the ant–plant Cordia nodosa is able to apply retaliatory sanctions against its ant symbiont Allomerus octoarticulatus, which patrols new shoots to prevent herbivory. We test the hypothesis that the modular design of C. nodosa physiologically ties the growth of housing (stem swellings known as domatia) to the successful development of the attached leaves. We experimentally simulated herbivory by cutting leaves from patrolled shoots and found that the domatia on such ‘cheated’ shoots suffered higher mortality and lower growth than did controls, evidence for a host sanction. On the other hand, patrolling is costly to the ant, and experiment shows that non-patrollers run a low risk of being sanctioned because most leaves (and the attached domatia) escape heavy herbivory even when patrollers are absent. This suggests that cheaters might enjoy a higher fitness than do mutualists, despite sanctions, but we find that patrolling provides a net fecundity benefit when the colony and plant exceed a minimum size, which requires sustained ant investment in patrolling. These results map directly onto the principal–agent (P–A) game from economics, which we suggest can be used as a framework for studying stability in mutualisms, where high sampling costs and cheating do not allow market effects to select for mutual benefits.
Knowledge of the role of ants in many agroecosystems is relatively scarce, and in temperate regions the possibility to exploit ants as biocontrol agents for crop protection is still largely unexplored. Drawing inspiration from mutualistic... more
Knowledge of the role of ants in many agroecosystems is relatively scarce, and in temperate regions the possibility to exploit ants as biocontrol agents for crop protection is still largely unexplored. Drawing inspiration from mutualistic ant–plant relationships mediated by extrafloral nectaries (EFNs), we tested the use of artificial nectaries (ANs) in order to increase ant activity on pear trees and to evaluate the effects on the arthropods, plant health and fruit production. While EFNs secrete a complex solution mainly composed of sugars and amino acids, ANs were filled with water and sucrose only. The results suggest that ANs can be used as manipulative instruments to increase ant activity over long periods of time. High ant activity was significantly linked to lower incidence of the pathogen fungus Venturia pyrina (pear scab) on pear leaves, and of the presence of Cydia pomonella (codling moth) caterpillars on pear fruit production. These results further encourage exploring und...
Fourteen microsatellite loci were isolated from the critically endangered Corfu toothcarp Valencia letourneuxi using a microsatellite enrichment protocol and selective hybridization with a biotinylated (AC) probe. Nine of these loci were... more
Fourteen microsatellite loci were isolated from the critically endangered Corfu toothcarp Valencia letourneuxi using a microsatellite enrichment protocol and selective hybridization with a biotinylated (AC) probe. Nine of these loci were polymorphic in a V. letourneuxi population of 20 individuals (Vlychos stream) with mean number of alleles per locus = 2.55 and mean observed heterozygosity = 0.3447. Moreover, some of these 9 microsatellite markers
