Aims: An ex vivo and in vivo study to determine various positions in the ovine pelvis to evaluate dental implant placement. Settings and Design: • A novel, unique surgical dissection was described as the caudal roll-up technique and was... more
Aims: An ex vivo and in vivo study to determine various positions in the ovine pelvis to evaluate dental implant placement. Settings and Design: • A novel, unique surgical dissection was described as the caudal roll-up technique and was utilized to obtain maximal exposure to the Linea Glutea, with the least morbidity. • Insertion torque (IT) of osseo-integrated dental implants was measured at predetermined positions described as A-F along the ovine linea glutea. • The amount of outer cortical bone was measured at predetermined sections A-F on harvested ovine pelvi, to coincide with the implant positions as placed. Statistical analysis used: Descriptive Study. Intraoral dental implant research in animals is challenging, owing in particular to different tooth morphology. One of the most challenging problems with intraoral implant research in animals is an infection form contaminated oral cavity, which is evident in intraoral research related to pigs and dogs [8]. Intraoral dental implant studies by Vlaminck confirmed that the specific oral biomechanics inherent to a sheep's constant ruminant activity accounted for high degrees of implant failures [13, 25]. A novel, unique surgical dissection was described as the caudal roll-up technique and was utilized to obtain maximal exposure to the Linea Glutea, with the least morbidity. Data collected concerning the insertion torque of implants placed and the cortical thickness measurements of the sheep's ilia was discussed.
Objective: The present paper aims to investigate the role of computed tomography as an imaging technique of diagnosis in the identification of the os cordis in ovine, and also how this anatomical structure is morphotopographically... more
Objective: The present paper aims to investigate the role of computed tomography as an imaging technique of diagnosis in the identification of the os cordis in ovine, and also how this anatomical structure is morphotopographically characterized in macro and microscopic contexts, seeking to contribute for its functional understanding. Materials and method: The heart of a young male ovine had been donated to the Laboratory of Animal Anatomy of the Surgery Department of the FMVZ/USP, first being subject to a post-mortem examination by means of the cardiac "shedding" (transverse cross sectioning of the heart). A tomographic examination of the anatomic specimen was carried out, as well as the dissection and histological analysis of the collected sample. Results: The results indicate the presence of an osseous structure of 6.39 mm in length, located in the aortic valve of the heart, next to a ring comprised by three valve flaps, tendinous cords, and papillary muscles. The histological findings consist of fibrous connective tissue, cancellous bone tissue, and calcified hyaline cartilage wherein the cardiomyocytes are attached. Conclusions: It is concluded that computed tomography, even though seldom applied to animals of zootechnical interest, poses as an effective tool for the visualization of the os cordis in lambs.
The relevance of low developmental competence of in vitro-matured oocyte to the incomplete/delayed cytoplasmic maturation, and the heterogeneity of retrieved oocytes is well established in several species. A short phase of prematuration... more
The relevance of low developmental competence of in vitro-matured oocyte to the incomplete/delayed cytoplasmic maturation, and the heterogeneity of retrieved oocytes is well established in several species. A short phase of prematuration culture was used to allow better oocyte cytoplasmic maturation. The preselection of growing and fully grown oocytes has been proposed to improve developmental competency. This study investigated the effects of phosphodiesterase type 3-specific inhibitor, cilostamide, and adenylate cyclase activator, forskolin, on the resumption of meiosis and developmental competence of growing ovine oocytes selected by brilliant cresyl blue (BCB) staining. Results indicate that cilostamide, forskolin, and their combination significantly (P < 0.05) increased the percentage of growing (BCBÀ) oocytes maintained at the germinal vesicle stage. However, only forskolin significantly (P < 0.05) increased the yield and quality of blastocysts derived from BCBÀ oocytes compared with non-BCB-treated oocytes. We conclude that a short prematuration culture with forskolin may improve the in vitro developmental competency of growing oocytes in ovine.
The official methods specified in the national bovine and ovine/caprine brucellosis eradication plan are the Rose Bengal and complement fixation tests. In the current phase of the eradication plan, it is often difficult to interpret the... more
The official methods specified in the national bovine and ovine/caprine brucellosis eradication plan are the Rose Bengal and complement fixation tests. In the current phase of the eradication plan, it is often difficult to interpret the results obtained with the official tests. Consequently, additional tests that offer greater sensitivity and specificity are thus required. For this reason, two methods, the indirect chemiluminescence enzyme-linked immunosorbent assay (i-ELISA CL) and the competitive chemiluminescence ELISA (c-ELISA CL) that use a chemiluminescent substrate to determine anti-Brucella antibodies in bovine and ovine serum were validated. The methods are based on the detection of anti-Brucella antibodies in serum by catalysis of a chemiluminescent enzyme substrate (luminol/H2O2/enhancer system) by peroxidase conjugated to secondary anti IgG antibodies in i-ELISA CL and to monoclonal anti-lipopolysaccharide (LPS) antibodies in c-ELISA CL. From the results obtained, a cut-...
The official methods specified in the national bovine and ovine/caprine brucellosis eradication plan are the Rose Bengal and complement fixation tests. In the current phase of the eradication plan, it is often difficult to interpret the... more
The official methods specified in the national bovine and ovine/caprine brucellosis eradication plan are the Rose Bengal and complement fixation tests. In the current phase of the eradication plan, it is often difficult to interpret the results obtained with the official tests. Consequently, additional tests that offer greater sensitivity and specificity are thus required. For this reason, two methods, the indirect chemiluminescence enzyme-linked immunosorbent assay (i-ELISA CL) and the competitive chemiluminescence ELISA (c-ELISA CL) that use a chemiluminescent substrate to determine anti-Brucella antibodies in bovine and ovine serum were validated. The methods are based on the detection of anti-Brucella antibodies in serum by catalysis of a chemiluminescent enzyme substrate (luminol/H2O2/enhancer system) by peroxidase conjugated to secondary anti IgG antibodies in i-ELISA CL and to monoclonal anti-lipopolysaccharide (LPS) antibodies in c-ELISA CL. From the results obtained, a cut-...
