ABSTRACTThe study was aimed to determine combination media and plant growt regulator to support growth of F1 of PLB orchid from Phalaenopsis gigantea x Phalaenopsis violacea hibridisation,. This research consist of two... more
ABSTRACTThe study was aimed to determine combination media and plant growt regulator to support growth of F1 of PLB orchid from Phalaenopsis gigantea x Phalaenopsis violacea hibridisation,. This research consist of two experiment. The first experiment use randomize complete block design with two factor i.e media and plant growth regulator (PGR). The experiment used Knudson C 80% and NPK (18:18:18+EDTA) media, while the plant growth regulator used without PGR, coconut water, thidiazuron (TDZ) + benziladenin (BA). The second experiment was subculture in different media, Knudson C 80% without PGR to Knudson C 80% without PGR and NPK, NPK (20:20:20)+vitamin+myoinositol; NPK (18-18-18+EDTA) without PGR subculture to NPK (18-18-18+EDTA) without PGR and NPK (20:20:20)+vitamin+myoinositol; NPK (18:18:18+EDTA) +TDZ 0.5 ppm+BA 0.5 ppm subculture to NPK (18:18:18+EDTA) +TDZ 0.5 ppm+BA 0.5 ppm andNPK (20:20:20)+ vitamin+myoinositol; KC 80%+c...
Gerbera (Gerbera jamesonii Bolus ex Hook) is the latest sensation to Indian Floriculture, commercially grown throughout the world in a wide range of climatic conditions (Lhoste, 2002). According to the global trends in... more
Gerbera (Gerbera jamesonii Bolus ex Hook) is the latest sensation to Indian Floriculture, commercially grown throughout the world in a wide range of climatic conditions (Lhoste, 2002). According to the global trends in floriculture, Gerbera occupies the 4th place among cut flowers (Sujatha et al., 2002). Gerbera, commonly known as Transvaal Daisy, Barberton Daisy or African Daisy, produces very attractive flowers. It is an important commercial flower grown throughout the world under a wide range of climatic conditions. This is ideal for beds, borders, pots and rock gardens. Flowers are of various colours and forms and suit very well in different floral arrangements. Cut-blooms, when placed in water, last for a long time.
Objective: Within the field of biorobotics, an emerging branch is plant-inspired robotics. Some effort exists in particular towards the production of digging robots that mimic roots; for these, a deeper comprehension of the role of root... more
Objective: Within the field of biorobotics, an emerging branch is plant-inspired robotics. Some effort exists in particular towards the production of digging robots that mimic roots; for these, a deeper comprehension of the role of root tip geometry in excavation would be highly desirable. Here we demonstrate a photogrammetry-based pipeline for the production of computer and manufactured replicas of moth orchid root apexes. Results: Our methods yields faithful root reproductions. This can be used either for quantitative studies aimed at comparing different root morphologies, or directly to implement a particular root shape in a biorobot.
The market for potted Phalaenopsis – the most common orchid genus sold in Europe – has steadily increased in volume and value over time. This paper aims to identify the value chain of potted Phalaenopsis. The governance of the value chain... more
The market for potted Phalaenopsis – the most common orchid genus sold in Europe – has steadily increased in volume and value over time. This paper aims to identify the value chain of potted Phalaenopsis. The governance of the value chain is investigated along with the possibilities of upgrading the chain through certification. The analysis is based on qualitative data from interviews with important actors involved in the value chains of potted Phalaenopsis in the Netherlands and in Germany. The results reveal that the value chains of potted Phalaenopsis in the Netherlands – the EU’s biggest producer – and Germany – the EU’s biggest consumer market – are strongly interlinked. Dutch growers, however, dominate the supply of the European market due to their well-organized marketing system, relatively low risk aversion and great flexibility in adjusting their production patterns quickly to the latest trends. Imports of potted Phalaenopsis from developing countries are not considered to be a threat. Currently there is little demand from retail customers for business-to-consumer certification in potted Phalaenopsis. For the present moment and the near future only business-to-business quality management systems are considered to be important in the potted Phalaenopsis value chain.
Phalaenopsis is one of the most remarkable commercial genera in Orchidaceae family. Using immature explants for tissue culture of orchid results in undesired characteristics such as genetic variation and long-lasting juvenility. In this... more
Phalaenopsis is one of the most remarkable commercial genera in Orchidaceae family. Using immature explants for tissue culture of orchid results in undesired characteristics such as genetic variation and long-lasting juvenility. In this study, we optimized a protocol for somatic embryogenesis of Phalaenopsis amabilis cv. Jinan, by using mature thin cell layer (TCL) explants to minimize immature explant limitations. We examined somatic embryogenesis from two types of mature explants, viz. leaf lTCL and flower stalk nodes tTCL at various concentrations of thidiazuron (TDZ) (0, 1, 2, and 3 mg L-1). The results demonstrated that lTCL explants cultured on medium supplemented with 3 mg L–1 TDZ produced the highest number of somatic embryos (SEs) (21.5 embryos per explant), the lowest yellowish SE (7%), the highest plantlet regeneration (20.5 plantlets per explant) and the highest survival rate (94%) in comparison with tTCL explants. This protocol can be used in commercial mass propagation of Phalaenopsis to overcome the limitations such as long-term juvenility and genetic variation of plantlets regenerated in vitro by using immature explants.
Phalaenopsis, one of plant genus in Orchidaceae, is commercially produced as pot plant and cut flower because it has high economical value in world market, including Indonesia. Several micropropagation methods have been developed for... more
Phalaenopsis, one of plant genus in Orchidaceae, is commercially produced as pot plant and cut flower because it has high economical value in world market, including Indonesia. Several micropropagation methods have been developed for Phalaenopsis, but not all of these methods can be used for commercial micropropagation because of differences in survival rate, PLB formation and plantlet regeneration. Therefore, continued studies about the most important stages in Phalaenopsis micropropagation, which are multiplication and germination stages, are needed. Combination of NAA and BAP produce PLB formation and multiplication. Objectives of this study were to determine the effect of NAA and BAP concentrations in PLB multiplication, and germination of PLB from this multiplication results grown in medium without plant growth regulators. Effects of NAA and BAP combinations, which are 0.1 ppm NAA and 1 ppm BAP; 0.1 ppm NAA and 5 ppm BAP; 0.1 ppm NAA and 10 ppm BAP; 0.1 ppm NAA and 20 ppm BAP; ...
The application of elicitors, which is currently the focus of research, has been considered as one of the most effective methods to improve the synthesis of secondary metabolites in plant tissue culture. This experiment was done to see... more
The application of elicitors, which is currently the focus of research, has been considered as one of the most effective methods to improve the synthesis of secondary metabolites in plant tissue culture. This experiment was done to see the effects of growth and development of Phalaenopsis 'Fmk02010' using two elicitors: N-Acetyl-D-glucosamine(NAG) and 5-aminolevulinic acid (5-ALA). The maximum average number of Protocorm-like bodies (PLBs) (24.3), highest shoot number (5.1) and fresh weight (0.335 g) was found from 0.1 mg/L NAG treatments. In case of 5-ALA, 0.1 mg/L 5-ALA supplementation in modified MS medium enhanced the best formation of PLBs (20.2), whereas the highest number of shoots (4.66) highest fresh weight (0.312 g) was found at 1mg/L compared with control.Elicitors are substances that induce protective responses in plants. Low concentration of NAG and 5-ALA has been suggested as a new natural and environmentally friendly regulator, which can be widely used in agriculture.
