The epidermis of the anterior end (nose) plays an important role in the evolution, development, and functional feeding morphology in nematodes, but information on this complex organ system is limited. Here, we produce a 3D model of 13 of... more
Les souches VIH-1 n'induisant pas la formation de syncytium (NSI) ont ete associees avec la transmission verticale de VIH-1 in utero, et avec l'infection de placentas humains in vitro dans une etude preliminaire utilisant trois... more
Les souches VIH-1 n'induisant pas la formation de syncytium (NSI) ont ete associees avec la transmission verticale de VIH-1 in utero, et avec l'infection de placentas humains in vitro dans une etude preliminaire utilisant trois souches virales. Pour etablir la relation existant entre le phenotype NSI et SI (induisant la formation de syncytium) de VIH-1 avec l'infection du placenta humain in vitro, nous avons utilise 21 souches differentes, en majorite de type sauvage provenant de bebes infectes in utero, intra-partum ou post-partum. Des explants placentaires villeux, de grossesses a terme non infectees, ont ete incubes durant 24 heures avec chacune des souches virales avant de subir un rincage intensif pour eliminer l'inoculum. Le tissu etait ensuite incube durant trois jours supplementaires. L'infection du tissu placentaire etait determinee par l'ADN viral amplifie par PCR (2 regions, Gag et LTR). Des 21 souches VIH-1, 38 % (8 sur 21) etaient capables d'infecter le tissu placentaire. 75 % etaient des souches NSI (6 sur 8) et 25 % des souches SI (2 sur 8). Sur le total des souches NSI, 40 % (6 sur 15) etaient infectieuses contre 33% (2 sur 6) du total des souches SI. En resume, l'infection VIH-1 du placenta humain a terme n'est pas uniquement dependante du phenotype NSI ou SI du VIH-1. De plus, toutes les souches VIH-1 isolees de bebes infectes in utero n'ont pas infecte les explants placentaires, alors que ces souches infectent les PBMC. D'autres caracteristiques de VIH-1 et du placenta lui-meme pourraient etre des elements importants de l'infectivite du placenta humain par VIH-1.
Human immunodeficiency virus 2 (HIV-2) ISY and the newly derived HIV-2KR are infectious molecular clones that yield viruses differing markedly in their abilities to infect and/or induce syncytia in various T- and monocytoid-cell lines.... more
Human immunodeficiency virus 2 (HIV-2) ISY and the newly derived HIV-2KR are infectious molecular clones that yield viruses differing markedly in their abilities to infect and/or induce syncytia in various T- and monocytoid-cell lines. Chimeric viruses were constructed from these two viral genomes to localize the genetic determinants of some of these properties. Envelope sequences, particularly those spanning the CD4 binding site, appear to be critical for the ability of HIV-2KR to infect MOLT-4 clone 8 and SupT1 cells and to efficiently infect the H9 cell line. On the other hand, multiple determinants may contribute to cytopathicity (gp41 and nef) in H9 cells and replication efficiency in monocytic (THP-1) cells.
•Soybean cyst nematodes (Heterodera glycines) produce secreted effector proteins that function as peptide mimics of plant CLAVATA3/ESR (CLE)-like peptides probably involved in the developmental reprogramming of root cells to form... more
•Soybean cyst nematodes (Heterodera glycines) produce secreted effector proteins that function as peptide mimics of plant CLAVATA3/ESR (CLE)-like peptides probably involved in the developmental reprogramming of root cells to form specialized feeding cells called syncytia.•The site of action and mechanism of delivery of CLE effectors to host plant cells by the nematode, however, have not been established. In this study, immunologic, genetic and biochemical approaches were used to reveal the localization and site of action of H. glycines-secreted CLE proteins in planta.•We present evidence indicating that the nematode CLE propeptides are delivered to the cytoplasm of syncytial cells, but ultimately function in the apoplast, consistent with their proposed role as ligand mimics of plant CLE peptides. We determined that the nematode 12-amino-acid CLE motif peptide is not sufficient for biological activity in vivo, pointing to an important role for sequences upstream of the CLE motif in function.•Genetic and biochemical analysis confirmed the requirement of the variable domain in planta for host-specific recognition and revealed a novel role in trafficking cytoplasmically delivered CLEs to the apoplast in order to function as ligand mimics.Soybean cyst nematodes (Heterodera glycines) produce secreted effector proteins that function as peptide mimics of plant CLAVATA3/ESR (CLE)-like peptides probably involved in the developmental reprogramming of root cells to form specialized feeding cells called syncytia.The site of action and mechanism of delivery of CLE effectors to host plant cells by the nematode, however, have not been established. In this study, immunologic, genetic and biochemical approaches were used to reveal the localization and site of action of H. glycines-secreted CLE proteins in planta.We present evidence indicating that the nematode CLE propeptides are delivered to the cytoplasm of syncytial cells, but ultimately function in the apoplast, consistent with their proposed role as ligand mimics of plant CLE peptides. We determined that the nematode 12-amino-acid CLE motif peptide is not sufficient for biological activity in vivo, pointing to an important role for sequences upstream of the CLE motif in function.Genetic and biochemical analysis confirmed the requirement of the variable domain in planta for host-specific recognition and revealed a novel role in trafficking cytoplasmically delivered CLEs to the apoplast in order to function as ligand mimics.
The epidermis of the anterior end (nose) plays an important role in the evolution, development, and functional feeding morphology in nematodes, but information on this complex organ system is limited. Here, we produce a 3D model of 13 of... more
The epidermis of the anterior end (nose) plays an important role in the evolution, development, and functional feeding morphology in nematodes, but information on this complex organ system is limited. Here, we produce a 3D model of 13 of the cells making up this organ system reconstructed from serial transmission electron micrographs of the microbial feeding nematode, Acrobeles complexus. Nose epidermal cells were found to be broadly similar to those of the distantly related model organism Caenorhabditis elegans in the number and arrangement of nuclei in these largely syncytial cells; this similarity demonstrates striking evolutionary conservation that allows for robust statements of homology between the taxa. Examining details of cell shape, however, revealed surprisingly complex subcellular specialization, which differed markedly from C. elegans in the number and arrangement of cell processes. Anterior toroid processes of the anterior arcade, posterior arcade, and HypB syncytia fo...
Viral haemorrhagic septicaemia virus (VHSV) belongs to the rhabdovirus family and is a major pathogen in farmed rainbow trout. An insect cell culture traditionally used for production of recombinant proteins was found to be susceptible to... more
Viral haemorrhagic septicaemia virus (VHSV) belongs to the rhabdovirus family and is a major pathogen in farmed rainbow trout. An insect cell culture traditionally used for production of recombinant proteins was found to be susceptible to VHS virus. At pH 6.2, VHSV multiplication induced formation of large syncytia similar to those obtained by baculovirus-induced expression of recombinant VHSV glycoprotein. The VHSV G protein produced in insect cells was smaller than G protein derived from fish cells. VHS virus produced in insect cells was still pathogenic to rainbow trout after 2 cell culture passages.