The Trivedi Effect®

Chromium trioxide (CrO3) has many industrial applications for the manufacturing of aerospace parts, synthetic rubies, stripping agent, etc. but it is very hygroscopic, toxic and powerful oxidizing material. The objective of this study was to evaluate the influence of the Trivedi Effect®-Consciousness Energy Healing Treatment on the physicochemical and thermal properties of CrO3 using modern analytical techniques. The sample was divided into two parts, one part of the test sample was considered as the control sample (no Biofield Treatment was provided), while another portion received the Biofield Treatment remotely by a renowned Biofield Energy Healer, Gopal Nayak and termed as the treated sample. The particle size values of the treated CrO3 were increased by 2.39%, 4.52%, 4.42%, and 4.15% at d10, d50, d90, and D(4,3), respectively compared to the control sample. Thus, the specific surface area of the treated CrO3 was decreased by 4.27% compared with the control sample. The PXRD peak intensities of the treated CrO3 were significantly altered ranging from -73.48% to 212.5% compared with the control sample. Similarly, the crystallite sizes of the treated sample were significantly altered ranging from -38.7% to 167.39% compared to the control sample. However, the average crystallite size of the treated sample was increased by 4.25% compared with the control sample. The latent heat of fusion of the treated sample was significantly increased by 556.45% compared with the control sample. The Trivedi Effect®-Consciousness Energy Healing Treatment might produce a new polymorphic form of CrO3 which would be better powder flowability and lower solubility; hence it will show the lower absorption and toxicity on inhalation, ingestion, contact to skin and eye, chronic exposure, and aggravation of pre-existing conditions. The Biofield Energy Treated CrO3 sample would be very useful for the ceramic industry manufacturing the synthetic rubies, stripping agent of anodic coatings, aerospace parts, etc.

The aim of the present study was to determine the impact of Biofield Energy Treated test formulation using six different cell-lines. The test formulation/item (TI) and cell media (Med) was divided into two parts; one part was untreated (UT) and other part received Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Janice Patricia Kinney, USA and labeled as Biofield Energy Treated (BT) test item (TI)/ media. Based on cell viability assay, test formulation was found as safe at tested concentrations. Cytoprotective activity of test formulation showed a significant restoration of cell viability by 60.6% (10 µg/mL), 67.5% (63.75 µg/mL), and 117.5% (63.75 µg/mL) in UT-Med + BT-TI, BT-Med + UT-TI, BT-Med + BT-TI, respectively compared to untreated in human cardiac fibroblasts cells (HCF) cells. Moreover, restoration of cell viability was improved by 64% and 127.3% in UT-Med + BT-TI and BT-Med + UT-TI, respectively at 1 µg/mL compared to untreated in human liver cancer (HepG2) cells. Cellular restoration in A549 cells was improved by 314% and 112.3% at 1 µg/mL in BT-Med + UT-TI and BT-Med + BT-TI, respectively than untreated. ALP activity in Ishikawa cells was significantly increased by 175.5%, 547.2%, and 220.8% in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively at 0.1 µg/mL as compared to untreated. Additionally, in MG-63 cells showed increased ALP activity by 76.9%, 78.4%, and 79% in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively at 50 µg/mL compared to untreated. The percent cellular protection of HCF (heart) cells (decreased of LDH activity) was significantly increased by 60.6% (10 µg/mL), 67.5% (63.75 µg/mL), and 117.5% (63.75 µg/mL) in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively as compared to untreated. An improved HepG2 cells protection (represents decreased ALT activity) by 115.1% (1 µg/mL), 42.5% (25.5 µg/mL), and 60.8% (10 µg/mL) in UT-Med + BT-TI, BT-Med + UT-TI, BT-Med + BT-TI, respectively as compared to untreated. Percentage cellular protection of A549 (lungs) cells (represents increased of SOD activity) was significantly increased by 191.1% and 81.4% at 0.1 µg/mL in UT-Med + BT-TI and BT-Med + BT-TI, respectively as compared to untreated. Serotonin level was significantly increased by 31.8% (10 µg/mL) and 56.9% (25.5 µg/mL) in UT-Med + BT-TI and BT-Med + BT-TI, respectively compared to untreated in human neuroblastoma cells (SH-SY5Y). Relative quantification (RQ) of vitamin D receptor (VDR) was significantly increased by 304.3% (0.01 µg/mL), 128.4% (0.1 µg/mL), and 240% (0.1 µg/mL) in UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI, respectively compared to untreated in MG-63 cells. Thus, Biofield Energy Treated test formulation (The Trivedi Effect®) significantly improved organ specific functional biomarkers and would be useful for multiple organs health related to coronary artery disease, arrhythmias, congenital heart disease, cardiomyopathy, cirrhosis, liver cancer, hemochromatosis, asthma, chronic bronchitis, cystic fibrosis, osteoporosis, etc.

The study objective was to evaluate the effect of Biofield Treated vitamin D3 and DMEM on bone health. The Test Items (TI) were divided into two parts. One part of each sample was received Consciousness Energy Healing Treatment by Janice Patricia Kinney and those samples were labeled as Biofield Energy Treated (BT) samples, while other parts of each sample were denoted as Untreated Test items (UT). The cell viability showed test samples were found as safe in the tested concentrations. ALP was significantly increased by 212.57% and 134.55% in UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 50 µg/mL, while 242.77% increased in BT-DMEM + UT-TI at 10 µg/mL compared to UT-DMEM + UT-TI group. Collagen was significantly increased by 172.04%, 293.55%, and 248.39% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 0.1 µg/mL compared to untreated group. Moreover, collagen was significantly increased by 99.25% and 172.93% in UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 1µg/mL, while increased by 156.38% and 145.64% in UT-DMEM + BT-TI and BT-DMEM + BT-TI groups, respectively at 10 µg/mL than untreated group. Besides, percent of bone mineralization was distinctly increased by 198.62%, 111.72%, and 168.28% in UT-DMEM + BT-TI, BT-DMEM + UT-TI, and BT-DMEM + BT-TI groups, respectively at 0.1µg/mL compared to untreated group. Overall, Biofield Treated vitamin D3 was remarkably improved the bone health parameters and could be beneficial against bone-related disorders (osteoporosis, osteomalacia etc.), stress, inflammatory and autoimmune disorders.

Withania somnifera (ashwagandha) root extract is very popular ancient herbal medicine. The objective of the study was to characterize and evaluate the impact of The Trivedi Effect®-Biofield Energy Healing Treatment (Energy of Consciousness) on phytoconstituents present in the ashwagandha root extract using LC-MS. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received Energy of Consciousness Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The LC-MS analysis of the control and treated samples showed a very close retention time (Rt), indicated that the polarity of the phytoconstituents present in the root extract are same. The numbers of peaks observed in the total ion chromatograms were 28 and 29 in the control and treated samples, respectively. The change in the peak height% of the phytoconstituents in the treated sample was altered significantly within the range of -50.91% to 118.12% compared with the control sample. Similarly, the change in the peak area% of most of the phytoconstituents in the treated ashwagandha was significantly altered within the range of -54.95% to 66.95% compared with the control sample. An additional peak was appeared in the treated sample at Rt of 5.72 minutes, which was not found in the control sample. The LC-MS spectra indicated the presence of possible withanolides like -hydroxy-2,3-dihydro-withanolide F, withanolide A, withaferine A, withanone, withanolide D, ixocarpalactone A, withanolide S, thiowithanolide, etc. in both the samples. The peak are percentage (%) was altered in the identified withanolides, but withanolide sulfoxide was increased significantly by 12.44% in the treated sample compared with the control sample. These results indicated that The Trivedi Effect® - Biofield Energy Treatment might have an impact on the intrinsic physicochemical properties of the phytoconstituents present in the ashwagandha root extract. This could be the probable cause of alteration in the peak height, peak area, and appearance of a new peak in the treated sample. As a result, the concentrations of the phytoconstituents altered in the treated sample compared with the control sample. The treated ashwagandha root extract would be helpful for designing better pharmaceutical/nutraceutical formulations which might be providing a better therapeutic response against autoimmune diseases, nervous and sexual disorders, infectious diseases, antiaging, diabetes, cancer, ulcer, immunological disorders, stress, arthritis, etc.

Osteoporosis is associated with increased mortality and significant economic and health burden now-a-days. The study was aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. The test items, were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Carola Marina Sand and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Various parameters were performed for the assessment of bone health such as ALP, collagen, and bone mineralization in human bone osteosarcoma cells (MG-63). The cell viability (MTT) data showed that the test samples were found as safe in the tested concentrations. The level of ALP was significantly increased by 420.64%, 311.08%, and 532.17% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 1µg/mL compared to the UT-DMEM + UT-Test item group. Further, the ALP level was significantly elevated by 213.69%, 135.46%, and 42.76% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 10µg/mL compared to the untreated. Collagen was significantly increased by 228.22%, 185.40%, and 256.69% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 0.1µg/mL compared to the untreated. Further, the collagen level was significantly increased by 88.61% and 130.65% in the BT-DMEM + UT-Test item and BT-DMEM + BT-Test item, respectively at 1µg/mL compared to the untreated. Besides, the percent of bone mineralization was distinctly increased by 118.35%, 266.94%, and 118.25% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item groups, respectively at 50µg/mL compared to the untreated. The percent of bone mineralization was distinctly increased by 88.29%, 288.75%, and 86.25% in the UT-DMEM + BT-Test item, BT-DMEM + UT-Test item, and BT-DMEM + BT-Test item, respectively at 100µg/mL compared to the untreated. Overall, the Biofield Energy Treated vitamin D3 was tremendously improved the bone health parameters and it could be a powerful alternative nutraceutical supplement to combat vitamin D3 deficiency and fight against various bone-related disorders including osteoporosis, low bone density, osteogenesis imperfecta, Paget’s disease, rickets, osteomalacia, deformed bones, chondrodystrophia fetalis, autoimmune and inflammatory diseases, stress management and prevention, and anti-aging by improving overall health.

The stable isotope ratio analysis is widely used in several scientific fields such as agricultural, food authenticity, biochemistry, metabolism, medical research, etc. Resorcinol is one of the most versatile chemicals used for the synthesis of several pharmaceuticals, dyes, polymers, organic compounds, etc. The current research work was designed to investigate the impact of the biofield energy treatment on the isotopic abundance ratios of 13C/12C or 2H/1H or 17O/16O (PM+1/PM) and 18O/16O (PM+2/PM) in resorcinol using Gas chromatograph - mass spectrometry (GC-MS) technique. Resorcinol was divided into two parts - one part was control and another part was considered as biofield energy treated sample. The biofield energy treatment was accomplished through unique biofield energy transmission by Mr. Mahendra Kumar Trivedi (also called as The Trivedi Effect®). T1, T2, T3, and T4 were denoted by different time interval analysis of the biofield treated resorcinol in order to understand the influence of the biofield energy treatment on isotopic abundance ratio with respect to the time. The GC-MS spectra of the both control and biofield treated resorcinol exhibited the presence of molecular ion peak [M+] at m/z 110 (calculated 110.04 for C6H6O2) along with major fragmented peaks at m/z 82, 81, 69, 53, and 39. The relative peak intensities of the fragmented ions in biofield treated resorcinol (particularly T2) was significantly changed with respect to the control sample. The stable isotope ratio analysis in resorcinol using GC-MS revealed that the percentage change of the isotopic abundance ratio of PM+1/PM was increased in the biofield treated resorcinol at T1, T2, T3 and T4 by 1.77%, 165.73%, 0.74%, and 6.79%, respectively with respect to the control sample. Consequently, the isotopic abundance ratio of PM+2/PM in the biofield treated resorcinol at T2, T3, and T4 were enhanced by 170.77%, 3.08%, and 12.31%, respectively with respect to the control sample. Briefly, 13C, 2H, 17O contributions from (C6H6O2)+ to m/z 111 and 18O contribution from (C6H6O2)+ to m/z 112 for the biofield treated resorcinol at T2 and T4 were significantly altered as compared to the control sample. For this reasons, biofield treated resorcinol might exhibit altered physicochemical properties like diffusion velocity, mobility and evaporation rate, reaction rate, binding energy, and stability. Biofield treated resorcinol could be valuable in pharmaceutical and chemical industries as intermediates during the preparation of pharmaceuticals and chemical compounds by altering its physicochemical properties, the reaction rate and selectivity, the study of the reaction mechanism and facilitating in designing extremely effective and specific enzyme inhibitors.

p-tert-Butyphenol (PTBP) is a phenolic monomer used in the synthesis of numerous industrially useful chemicals. The current research work aimed to evaluate the effect of the biofield energy treatment on the isotopic abundance ratios of PM+1/PM and PM+2/PM in PTBP using gas chromatography - mass spectrometry (GC-MS). The sample, PTBP was distributed into two parts - one part was designated as control PTBP and another part was considered as biofield energy treated PTBP. The biofield energy treatment was achieved through unique biofield energy transmission process by Mr. Trivedi (also known as The Trivedi Effect®). T1, T2, T3, and T4 were indicated to the different time interval analysis of the biofield treated PTBP. The GC-MS spectra of the both control and biofield treated PTBP showed the presence of molecular ion peak [M+] at m/z 150 (calculated 150.10 for C10H14O) along with eight major fragmented peaks at m/z 135, 107, 95, 91, 77, 65, 41, and 39, which might be due to C10H15+, C7H7O+ or C8H11+, C6H7O+, C7H7+, C6H5+, C5H5+, C3H5+, and C3H3••+ ions, respectively. The relative intensities of the parent molecule and other fragmented ions of the biofield treated PTBP were altered as compared to the control PTBP. The percentage in the isotopic abundance ratio of PM+1/PM was enhanced in the biofield treated PTBP at T2, T3 and T4 by 1.60%, 3.57%, and 120.13%, respectively while it was decreased by 4.14% in the treated sample at T1 with respect to the control PTBP. Consequently, the isotopic abundance ratio of PM+2/PM was increased in the biofield treated PTBP at T1, T3, and T4 by 1.28%, 2.56%, and 123.08%, respectively with respect to the control sample. On the other hand, it was reduced in the biofield treated sample at T2 by 1.28% as compared to the control PTBP. Concisely, 13C, 2H, and 17O contributions from (C10H14O)+ to m/z 151 and 18O contribution from (C10H14O)+ to m/z 152 in the biofield treated PTBP were changed with respect to the control sample and was found to have time dependent effect. The biofield energy treated PTBP might display isotope effects such as different physicochemical and thermal properties, rate of the reaction, selectivity and binding energy due to the changed isotopic abundance ratio as compared to the control sample. Biofield treated PTBP could be valuable for the designing new chemicals and pharmaceuticals through using its kinetic isotope effects.

Thymol is a natural monoterpenoid phenol possessing various pharmacological activities such as antimicrobial, antioxidant, etc. The stable isotope ratio analysis has drawn attention in numerous fields such as agricultural, food authenticity, biochemistry, metabolism, medical research, etc. An investigation of the effect of the biofield energy treatment (The Trivedi Effect®) on the isotopic abundance ratios of PM+1/PM and PM+2/PM in thymol using gas chromatography - mass spectrometry was attempted in this study. The sample, thymol was divided into two parts - one part was denoted as control and another part was referred as biofield energy treated sample that was given Mr. Trivediꞌs unique biofield energy. T1, T2, T3, and T4 were represented to different time interval analysis of the biofield treated thymol. The GC-MS spectra of the both control and biofield treated thymol indicated the presence of molecular ion peak [M+] at m/z 150 (calculated 150.10 for C10H14O) along with the similar pattern of fragmentation. The relative intensities of the parent molecule and other fragmented ions of the biofield treated thymol were enhanced as compared to the control thymol. The percentage change of the isotopic abundance ratio of PM+1/PM in the biofield treated thymol at T1, T2, T3 and T4 was increased by 3.25, 6.31, 96.75, and 140.25%, respectively as compared to the control thymol. In addition, the percentage change of the isotopic abundance ratio of PM+2/PM was increased in the biofield treated thymol at T1, T2, T3, and T4 by 5.33, 8.00, 101.33, and 140.00%, respectively with respect to the control sample. In summary, 13C, 2H, and 17O contributions from (C10H14O)+ to m/z 151 and 18O contribution from (C10H14O)+ to m/z 152 in the biofield treated thymol were significantly increased gradually with respect to the time and was found that biofield energy treatment has time dependent effect on it. Hence, the biofield energy treated thymol might display altered isotope effects such as physicochemical and thermal properties, binding energy and the reaction kinetics with respect to the control sample. So, biofield energy treated thymol could be advantageous for designing the synthetic scheme for the preparation of pharmaceuticals through its kinetic isotope effects. Besides, biofield treated thymol might be useful to overcome the problems associated with thymol for e.g. pungent flavor, high dose requirement for the activity through understanding its isotope effects and the determination of its pharmacokinetic profile, bioavailability.

The use of herbomineral formulations for different chronic diseases is gaining popularity because these natural formulations have fewer side effects, a higher safety profile, and more cost effectiveness. A new proprietary herbomineral formulation composed of herbs and certain minerals was formulated, consisting of the herbal root extract ashwagandha and the minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of a Biofield Energy Healing (The Trivedi Effect®) Treatment on the herbomineral formulation in female Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. Immunomodulatory potential, humoral immune response, paw volume, hematological study, biochemistry, body weight, feed intake and histopathology analysis were performed. Humoral immune response data showed increased (p≤0.01) primary antibody titre by 124.7% in the Biofield Treatment test formulation (G3), while the untreated test formulation group (G4) showed decreased antibody titre compared with the disease control group (G2). The secondary antibody titre value in the G3 group was also altered compared with the G2 group. Delayed type hypersensitivity (DTH) data suggest that the Biofield Energy Treated test formulation group (G3) showed a significant decrease in paw volume by 128.57% with respect to the G2 group. Hematology and biochemistry analysis showed altered values, as increased MCHC and RBC count by 138.09% and 7.24%, respectively in the G3 group compared with the G2 and G4 group. However, the platelet count was increased by 17.70% in the G3 group compared with the G2 group. The level of blood urea nitrogen was decreased by 10.90% in the G3 level compared with the G2 group. However, the levels of magnesium and phosphorus were increased by 13.29% and 6.28%, respectively in the G3 compared with the G2 group. Further, the change in body weight, feed consumption, organ to body weight ratio data, and histopathology examination did not suggest any statistical difference, which depicts that the Biofield Energy Treated test formulations was found to be safe. These data suggest that the Biofield Treated test formulation can be used for autoimmune and inflammatory diseases such as Rheumatoid arthritis, Alzheimer’s disease, Atherosclerosis, Dermatitis, Diverticulitis, Diabetes, etc. along with enhancing stress management and anti-aging by improving overall health.

The aim was to evaluate the impact of Biofield Treated vitamin D3 and DMEM on bone health. The test items (TI), were divided into two parts. One part of each sample received Consciousness Energy Treatment by Gopal Nayak and those samples were labeled as Biofield Treated (BT), while other parts of each sample were denoted as untreated test items (UT). MTT data showed test samples were found safe and nontoxic in tested concentrations. ALP was significantly increased by 213.46% and 218.66% in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 1 µg/mL compared to UT-DMEM + UT-TI. Moreover, ALP was significantly increased by 200.79% and 187.09% in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 10 µg/mL than untreated. Collagen synthesis was significantly increased by 551.25% and 130.31% in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 1 µg/mL than untreated. Collagen was significantly increased by 401.97% in BT-DMEM + UT-TI at 10 µg/ mL, while increased by 197.57% and 276.82% in UT-DMEM + BT-TI and BT-DMEM + UT-TI, respectively at 50 µg/mL than untreated. Percent bone mineralization was significantly increased by 186.86% and 188.23% in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 10 µg/mL than untreated. It was further increased significantly by 224.04% and 156.72% in BT-DMEM + UT-TI and BT-DMEM + BT-TI, respectively at 50 µg/ mL than untreated. Overall, data suggests that Biofield Treated vitamin D3 could be a potential alternative nutraceutical supplement to combat vitamin D3 deficiency and fight against various bone disorders.

Human dermal fibroblasts are defined for skin strength and resilience by continuously secreting extracellular matrix (ECM) components, which are responsible for promoting skin rejuvenation and remodeling. Precisely, the branched collagen fibrils provide the skin with its shape and firmness. The objective of the present study was to assess the potential of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco's Modified Eagle Medium) for evaluation of skin health parameters such as proliferation rate and collagen level using in vitro model. An increase in cellular proliferation and collagen levels were considered as markers for skin health promoting effects. An aliquot of HFF-1 cells and DMEM were divided into two equal parts, one was treated with the Biofield Energy Treatment and denoted as treated test sample, while other part was coded as the untreated test sample. MTT assay was used to study the cellular proliferation and collagen level was estimated by collagen dye complex formation using Biotek Synergy HT microplate reader. The HFF-1 cellular proliferation rate was significantly increased by 85% in the Biofield Energy Treated DMEM as compared with Biofield Treated cells. Similarly, the collagen level was significantly (p≤0.01) increased by 44.2% in the Biofield Energy Treated DMEM as compared with control. Hence, the result suggests a significant improvement of collagen level in the Biofield Energy Treated media (DMEM) for improving skin health. It can be concluded that The Trivedi Effect® might be a complementary and alternative approach with respect to the skin health, anti-aging activities in HFF-1 cell line.

The current research article was aimed to investigate the impact of The Trivedi Effect®- Energy of Consciousness Healing Treatment on the structural properties and isotopic abundance ratio (PM+1/PM and PM+2/PM) of magnesium gluconate using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts – one part was control, and another part was treated with The Trivedi Effect® remotely by eighteen renowned Biofield Energy Healers and defined as the Trivedi Effect® Treated sample. The liquid chromatogram of the control sample showed two peaks at Rt of 1.81 and 2.06 min, whereas the Trivedi Effect® Treated sample displayed these peaks at Rt of 1.79 and 2.03 min. The ESI-MS spectra of the control and the Trivedi Effect® Treated samples revealed the presence of the mass for magnesium gluconate ion in two forms at m/z 447 (adduct form with methanol) and 415 (protonated ion) in positive ionization mode. But, it showed the mass for the gluconate ion at m/z 195 in the negative ionization mode. The fragmentation pattern of magnesium gluconate in the treated sample was notably altered as compared to the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and the treated samples. The isotopic abundance ratio analysis revealed that the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in two magnesium gluconate ion forms at m/z 447 and 415 in treated sample was significantly decreased by 59.82% and 55.44%, respectively compared with the control sample. The percentage change in the isotopic abundance ratio of PM+2/PM (18O/16O or 26Mg/24Mg) was remarkably decreased in the magnesium gluconate ion at m/z 447 in the treated sample by 78.26% compared with the control sample. Consequently, the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in gluconate ion in the treated sample was significantly increased by 37.35% with respect to the control sample. Thus, the Trivedi Effect® treated magnesium gluconate could be valuable for designing better pharmaceutical and/or nutraceutical formulations through its changed physicochemical and thermal properties, which might be providing better therapeutic response against various diseases such as diabetes mellitus, allergies, aging, inflammatory diseases, immunological disorders, and other chronic infections. The treated magnesium gluconate might be helpful to design the novel potent enzyme inhibitors by using its kinetic isotope effects.

Withania somnifera (Ashwagandha) root extract is very popular ancient herbal medicine. The current study was designed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment on the structural characterization of the ashwagandha root extract using LC-MS, GC-MS, and NMR spectroscopy. Ashwagandha root extract was divided into two parts – one part was control, without any Biofield Energy Healing Treatment, while another part was treated with the Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as the Biofield Energy Treated sample. The retention time of the phytoconstituents remained same in both the control and treated samples, whereas the peak area% at respective retention time was significantly altered. The peak area% of the treated sample at Rt of 5.35, 5.55, 5.94, 6.25, 6.63, 6.76, 7.92, 8.04, 8.60, 8.73, and 9.31 min were significantly reduced by 6.15% to 60.67% compared with the control sample at Rt of 5.43, 5.65, 5.95, 6.29, 6.76, 6.85, 8.03, 8.14, 8.68, 8.78, and 9.30 min. Consequently, the peak area% of the treated sample at Rt of 7.25, 7.30, 8.27, and 8.47 min were significantly increased by 26.32%, 7.99%, 16.93% and 7.97% compared with the control sample at Rt of 7.37, 7.41, 8.36, and 8.55 min, respectively. A total of 13 withanolides were proposed with their structure from the deduced molecular mass at m/z 470, 472, 488, 504, 782, and 991 through LC-MS, GC-MS, 1H and 13C NMR analysis of both the control and treated samples. Viscosa lactone B, 27-hydroxy withanolide A, (20S, 22R)-α, 6α-epoxy-β, 5β, 27-trihydroxy-1-oxowitha-24-enolide, (20S, 22R)-4β, 5β, 6α, 27-tetrahydroxy-1-oxo-with-2, 24-dienolide were proposed in the control and treated samples at Rt of 6.85 and 7.30 min, respectively. Dihydrowithanolide D was only identified in the control sample at Rt of 7.41 min, whereas withanoside IV or withanoside VI was only present in the Biofield Treated sample at Rt of 6.76 min. Withanolide A, withaferin A, withanone, withanolide D, ixocarpalactone A and withanolide sulfoxide were found in both the control and treated samples. These findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment could be beneficial for altering the concentration of the phytoconstituents in the ashwagandha root extract by modifying their intrinsic physicochemical properties, which might be helpful to improve the bioavailability of active constituents of W. somnifera extract that might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.

The potential of The Trivedi Effect®-Consciousness Energy Healing vitamin D3 and DMEM medium was investigated in human bone osteosarcoma cells (MG-63). The Test Items (TI) i.e. vitamin D3 and DMEM were separated into two parts. Bone health parameters were tested such as Alkaline Phosphatase enzyme (ALP) activity, collagen levels and bone mineralization. The Test Items (TI) i.e. vitamin D3 and DMEM medium were divided into two parts. The test samples received Consciousness Energy Healing Treatment by Karen Byrnes Allen and samples were defined as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the Untreated test items (UT). Cell viability using MTT assay exhibited increased cell viability more than 71% with safe and nontoxic profile among test samples on MG-63 cell line. The level of ALP was significantly increased by116.4% at 50µg/mL in the UT-DMEM+BT-TI group, while 35.6%, 81.2%, and 47.9% at 10, 50 and 100 μg/mL, respectively in the BT-DMEM+UT-TI group. In addition, ALP level was increased by 42.3% and 90.6% at 10 and 50 μg/mL, respectively in the BT-DMEM+BT-TI groups as compared with the untreated group. The level of collagen was significantly increased by 10.5% and 110.1% at 0.1 and 1 μg/mL, respectively in the UT-DMEM+BT-TI group, while 22.6% and 190.6% at 0.1 and 1µg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased collagen level by 140.6% and 39.9% at 0.1 and 1 µg/mL, respectively as compared with the untreated test item and DMEM group. The percent of bone mineralization was significantly increased by 128.7% at 50 μg/mL, in the UT-DMEM+BT-TI group, while 34.1%, 154.7%, and 18.7% at 1, 10, and 50 μg/mL, respectively in the BT-DMEM+UT-TI group as compared with the untreated group. In addition, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 105.7%, 78.1%, and 39.6% at 1, 10, and 50 μg/mL, respectively as compared with the untreated group. Thus, the study results concluded that The Trivedi Effect® Treatment would be the best alternative treatment for the maintenance of strong and healthy bones and quality of life. Further, it regulates the osteoblast function and improved the level of collagen, ALP, and calcium absorption in wide range of bone disorders along with wide range of adverse bone health conditions.

The objective of the present study was to evaluate the immunomodulatory effect of Biofield Energy Treatment (The Trivedi Effect®) on Dulbecco’s Modified Eagle’s Medium (DMEM) and fetal bovine serum (FBS) in murine macrophage cells (RAW 264.7). The study parameters were evaluated using cell viability by MTT assay and estimation of proinflammatory cytokine like tumor necrosis factor - alpha (TNF-α) on immunomodulation using enzyme linked immune sorbent assay (ELISA). The cell viability using MTT assay data showed that more than 80% cell viability was observed in all the tested groups compared to the baseline control group (G1). The characteristics of cell morphology in the Biofield Energy Treated DMEM without FBS (G6) group showed some changes in the cell morphology as evidenced a transition from spherical to elongate and also showed maturation compared to the G1 group. The level of TNF-α expression was significantly reduced by 23.15% in the G3 group compared to the G1 group. Moreover, the secretion of TNF-α was altered by 38.06%, 35.77%, and 282.49% in the G4, G5, and G6 groups, respectively compared to the G1 group. The overall results demonstrated that the Trivedi Effect® - Consciousness Energy Healing Treatment has an impact on DMEM and FBS by alteration of cell morphology and reducing the level of TNF-α expression in immune cell (RAW 264.7). Therefore, the Trivedi Effect® treated DMEM and FBS might be useful as an immunomodulator for various immune-related disorders like Graves’ disease, rheumatoid arthritis, multiple sclerosis, etc.

Berberine is one of the well-known traditional Chinese medicines and it has been significantly effective against various ailments. This study was performed to evaluate the effect of the Trivedi Effect®-Consciousness Energy Healing Treatment on its physicochemical properties by using PXRD, PSA, TGA/DTG, and DSC analytical techniques. The sample was divided into two parts; the first part was considered as a control sample (without treatment), while the second part was received the Consciousness Energy Healing Treatment remotely by the Biofield Energy Healer, Gopal Nayak and denoted as the treated sample. The PXRD peak intensities and crystallite sizes were altered ranging from -28.78% to 115.15% and -15.76% to 241.38%, respectively; however the average crystallite size was increased significantly by 23.05% in the treated sample compared to the control sample. The particle sizes were significantly increased by 16.89% (d10), 34.41% (d50), 21.88% (d90), and 22.85 % {D (4,3)}, which resulted in the significant increase in the specific surface area by 14.74% in the treated sample compared to the control sample. The thermal analysis indicated 3.98% increase in weight loss and 3.64% decrease in the residue weight of the treated sample compared to the control sample. The latent heat for the 1st, 2nd, 3rd, 4th, 5th, and 6th peaks of the treated sample were significantly altered by 224.78%, 7.93%, 8.92%, -9.05%, -45.82%, and 10.93%, respectively, compared to the control sample. The overall analysis indicated that the Trivedi Effect®-Consciousness Energy Healing Treated berberine chloride might possess better solubility, absorption, and thermal stability compared to the untreated sample. Thus, the Trivedi Effect® could be used in improving the bioavailability of berberine chloride and might be used for designing the novel nutraceutical/pharmaceutical formulations to combat various diseases.

Withania somnifera (Ashwagandha) root extract possesses a broad range of pharmacological activities. The aim of current study was to explore the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing Treatment) on the physical, spectroscopic, and thermal properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts. One part was denoted as the control, while the other part was defined as The Trivedi Effect® - Biofield Energy Treated sample, which received The Trivedi Effect® Treatment remotely from eighteen renowned Biofield Energy Healers. The PXRD analysis exhibited that both the control and The Trivedi Effect® - Biofield Energy Treated samples were amorphous in nature. The particle size values at d10 and d50 of the treated sample were decreased by 4.66% and 6.02% and at d90 slightly increased by 0.99% compared with the control sample. The average surface area of the treated (0.130 m2/g) sample was increased by 4.84% compared with the control (0.124 m2/g) sample. The FT-IR results suggested that the force constant of O-H (stretching), and C-O (stretching) functional groups of the treated sample were reduced compared with the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 205.4 nm and 205.6 nm, respectively. The TGA thermograms exhibited three steps of the degradation process, and the total weight loss of the treated (86.45%) sample was increased by 1.44% compared with the control (85.22%) sample. The DSC analysis revealed that melting point of the treated (201.67°C) sample was reduced by 0.87% compared with the control (203.43°C) sample. The latent heat of fusion of the treated (6.77 J/g) sample was significantly decreased by 21.82% compared to the control (8.67 J/g) sample. Hence, the treated sample would be more advantageous for the better therapeutic responses compared to the control sample. These findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment might have the astounding capacity to enhance the solubility, dissolution, absorption, and bioavailability of ashwagandha root extract in various forms of pharmaceutical/nutraceutical formulations by modifying its particle size and surface area. Thus, The Trivedi Effect® - Energy of Consciousness Healing Treated ashwagandha root extract might provide better therapeutic response against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.

Bone disorders dramatically affecting the functional status of many individuals, which are suffering from bone diseases such as fractures, significant pain and height loss, disability to stand up, and walk. Vitamin D play an important role to improve the patients' quality of life with respect to bone disorders. The current study aimed to evaluate the potential of The Trivedi Effect®- Biofield Energy Healing on vitamin D3 and DMEM as test item (TI) on bone cell differentiation using human osteoblast cell line (MG-63, Osteosarcoma). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. The test items were treated with The Trivedi Effect® by Victoria Lee Vannes and divided as Biofield Energy Treated (BT) and untreated (UT) test items. Cell viability using MTT data showed that the test items were found to be safe. ALP level was significantly increased by 114.3%, 304.8%, and 314.3% at 0.1 µg/mL in the UT-DMEM+BT-TI, BT-DMEM+UT-TI, and BT-DMEM+BT-TI groups, respectively as compared to the untreated group. Collagen content was significantly increased by 82.5%, 138.4%, and 100.8% at 0.1, 1, and 10 µg/mL, respectively in the UT-DMEM+BT-TI, while 120.6% and 64.6% increased collagen at 0.1 and 1 µg/mL in BT-DMEM+UT-TI group and 261.9%, 179.8%, and 116.0% increased collagen in BT-DMEM+BT-TI group at 0.1, 1, and 10 µg/mL, respectively as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 261.2% and 239.9% at 1 µg/mL UT-DMEM+BT-TI and BT-DMEM+UT-TI groups, respectively as compared with the untreated group. However, BT-DMEM+BT-TI group showed a significant increased bone mineralization by 324.5% at 50 µg/mL. Thus, Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.

The use of herbomineral formulations in the healthcare sector has increased due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a combination of the herbal root extract ashwagandha and minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the test formulation in splenocytes. The test formulation was divided into two parts; one was the control without the Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers. The MTT assay showed that the test formulation exhibited safe concentrations up to 1.053 µg/mL with cell viability ranging from 88.19% to 110.30% in the Biofield Energy Treated sample. The Biofield Energy Healing significantly enhanced the cell viability as compared with the untreated test formulation. The expression of TNF-α was significantly inhibited by 7.23% at 1.053 µg/mL in the Biofield Energy Treated test formulation compared with the vehicle control. The level of TNF-α was significantly decreased by 3.90%, 11.74%, 3.12%, and 9.17% at 0.001053, 0.01053, 0.1053 and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation compared with the untreated test formulation. Additionally, the expression of IL-1β was significantly reduced by 28.98%, 51.23%, 53.06%, 48.98%, 55.71%, and 59.10% at 0.00001053, 0.0001053, 0.001053, 0.01053, 0.1053, and 1.053 µg/mL, respectively in the Biofield Energy Treated test formulation compared with the vehicle control. Further, the Biofield Treated test formulation showed significant reduction of IL-1β by 35.07% (p≤0.05), 47.46% (p≤0.05), and 57.51% (p≤0.01) at 0.001053, 0.1053, and 1.053 µg/mL, respectively compared with the untreated test formulation. Similarly, the MIP-1α expression was inhibited by the Biofield Energy Treated formulation and showed immunosuppressive activity at 0.00001053, 0.0001053, 0.001053, and 0.01053 µg/mL by 19.38%, 24.97%, 31.23%, and 25.41%, respectively compared with the vehicle control group. The Biofield Treated test formulation significantly (p<0.001) reduced the MIP-1α expression by 19.33%, 22.57%, and 30.50% at 0.0001053, 0.001053, and 0.01053 µg/mL, respectively compared with the untreated test formulation. Overall, The Trivedi Effect® - Biofield Energy Healing (TEBEH) significantly down-regulated the pro-inflammatory cytokines and potentiated the immunosuppressive effect of the Biofield Energy Treated test formulation, which can be better utilized for organ transplants, autoimmune diseases, inflammatory disorders, anti-aging, stress management, overall health and quality of life, etc.

Herbomineral formulations have increased in recognition and popularity due to their high safety and better therapeutic action. A new proprietary herbomineral formulation was formulated with a mixture of the herbal root extract of ashwagandha and three minerals viz. zinc, magnesium, and selenium. The aim of the study was to evaluate the immunomodulatory potential of Biofield Energy Healing (The Trivedi Effect®) on the test formulation when applied to splenocyte cells isolated from the Biofield Treated mice. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment. The other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely by seven renowned Biofield Energy Healers. A wide concentration range (0.00001053 to 10.53 µg/mL) of the test formulation was used to determine non-cytotoxic concentrations using MTT assay. Further, the expression of pro-inflammatory cytokines (TNF-α, MIP-1α, and IL-1β) was determined by ELISA method. The test formulation was evaluated and found to be safe up to 1.053 µg/mL with a percentage cell viability range of 73% to 97% using MTT assay. The Biofield Treated formulation improved the cell viability up to 6.61% compared with the untreated test formulation. TNF-α expression was significantly inhibited by 16.72% at 0.1053 µg/mL compared with the untreated test formulation, however expression was significantly altered by 53.67% and 25.62% at 0.01053 and 1.053 µg/mL, respectively compared to the untreated test formulation. TNF-α expression was also suppressed in the Biofield Treated test formulation at 0.001053 and 0.1053 µg/mL by 4.0% and 8.56%, respectively as compared with the vehicle control. MIP-1α suppression was reported in the Biofield Treated test formulation at 0.00001053 to 1.053 µg/mL by 8.43%, 22.02%, 21.92%, 20.54%, 5.40%, and 19.82%, respectively compared with the vehicle control. However, the Biofield Treated formulation further exhibited substantial suppression of MIP-1α at 0.0001053, 0.001053, 0.01053, and 0.1053 µg/mL by 13.50%, 7.38%, 36.83% (p≤0.001), and 2.53%, respectively compared with the untreated test formulation. In addition, significant inhibition of IL-1β secretion was reported in the Biofield Treated formulation at 0.0001053, 0.001053, 0.01053, and 0.01053 µg/mL by 32.40%, 14.99%, 60.42%, and 15.15%, respectively compared with the untreated test formulation. The Biofield Energy Healing Treatment significantly potentiated the immunosuppressive effect of the test formulation in Biofield Treated mouse splenocytes, which can be used for autoimmune and inflammatory diseases, stress management and anti-aging by improving overall health.

The study was investigated to find out the impact of the Biofield Energy Treated test formulation on the function of vital organs viz. bones, heart, liver, lungs, and brain in various cell-based assays. The test formulation/test item (TI) and the cell media (Med) was divided into two parts; one untreated (UT) and other part received the Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Vicki Lynn Kindy, USA and was labeled as the Biofield Energy Treated (BT) test formulation/media. Cell viability data suggested that the test formulation was safe and non-toxic in nature in six different cells. The experimental groups of untreated medium (UT-Med) + Biofield Treated Test Item (BT-TI), BT-Med + UT-TI, and BT-Med + BT-TI groups showed 116.4% (at 1 µg/mL), 84.1% (at 63 µg/mL), and 98.5% (at 63 µg/mL) restoration of cell viability, respectively in human cardiac fibroblasts cells (HCF) compared to the UT-Med + UT-TI group. Moreover, UT-Med + BT-TI group showed 62.5%, 156.8%, and 82.4% restoration of cell viability at 0.1, 1, and 10 µg/mL, respectively in human hepatoma cells (HepG2) compared to untreated. Furthermore, 224.2% and 295.6% restoration of cell viability was observed in adenocarcinomic human alveolar basal epithelial cells (A549) by UT-Med + BT-TI group at 1 and 10 µg/mL, respectively compared to the untreated. The alkaline phosphatase (ALP) level was significantly increased by 85.3%, 72.2%, and 79.4% in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively at 10 µg/mL in human bone osteosarcoma cells (MG-63) compared to the untreated. Additionally, the level of ALP was significantly increased by161% (at 0.1 µg/mL), 84.5% (at 25 µg/mL), and 63.9%(at 50 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively in human endometrial adenocarcinoma cells (Ishikawa) compared to the untreated. The percent protection of HCF (heart) cells (decreased of LDH activity) was significantly increased by 189.7% (at 0.01 µg/mL) and 51.7% (at10 µg/mL) in the UT-Med + BT-TI and BT-Med + BT-TI groups, respectively compared to the untreated in HCF cells. The percent protection of HepG2 (liver) cells (decreased of ALT activity) was significantly increased by 95.1% (at 25 µg/mL), 63.2% (at 1 µg/mL), and 112% (at 63 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively compared to untreated in HepG2 cells. The percent protection of A549 (lungs) cells (increased of SOD activity) was significantly increased by 36.9% and 44.1% in the BT-Med + UT-TI and BT-Med + BT-TI groups, respectively at 0.1 µg/mL compared to untreated in A549 cells. Serotonin level was significantly increased by 199.5%, 168.7%, and 83.9% in the BT-Med + BT-TI group at 0.1, 1, and 10 µg/mL, respectively as compared to untreated in human neuroblastoma cells (SH-SY5Y). The relative quantification (RQ) of vitamin D receptor (VDR) was significantly increased by 205.2% (at 10 µg/mL), 189% (at 0.01µg/mL), and 315.6% (at 10 µg/mL) in the UT-Med + BT-TI, BT-Med + UT-TI, and BT-Med + BT-TI groups, respectively compared to the untreated in MG-63 cells. Overall, these results suggest that Biofield Treated test formulation significantly improved the bones, heart, liver, lungs, and brain-related functional enzyme biomarkers. Altogether data suggest that the Biofield Energy Treatment (The Trivedi Effect®) can be useful to protect and maintain the normal function of each vital organ such as lungs, liver, heart, brain, and bones. Therefore, The Trivedi Effect® can be used as a complementary and alternative therapy against several disorders such as coronary artery disease, heart attack, heart failure, arrhythmias, congenital heart disease, cirrhosis, cardiomyopathy, liver cancer, Wilson disease, hemochromatosis, pneumonia, asthma, chronic bronchitis, emphysema, osteoporosis, cystic fibrosis, etc.

Herbal based test formulations have been used in most of the healthcare settings since time immemorial. The present experimental cell line study was designed to evaluate the impact of the Biofield Energy Treatment on test formulation and different cell line mediums related with vital organs functioning. Cell lines that were specific to different organ systems were used in the study using standard protocols. The Test Item (TI) and specific cell line media (Med) was divided into two parts; one untreated (UT-TI) and other part received the Biofield Energy Treatment remotely by a renowned Biofield Energy Healer, Eileen Mary Meagher, USA and were labeled as the Biofield Energy Treated (BT) test formulation/media. MTT assay was used for cell viability assay, and the results showed that the test item was found non-toxic. Cytoprotective action of the test formulation showed a significant maximum restoration of cell viability by 56.8% (at 63.75µg/mL), 57.5% (at 63.75µg/mL), and 124.8% (at 1µg/mL) in the UT-Med+BT-TI, BT-Med+UT-TI, and BT-Med+BT-TI groups respectively, as compared to the untreated test group in Human Cardiac Fibroblasts cells (HCF) cells, while 83.2% (at 63.75µg/mL), 93.8% (at 63.75µg/mL), and 20.6% (at 10µg/mL) improved cellular protection of human Hepatoma cells (HepG2) cells in the UT-Med+BT-TI, BT-Med+UT-TI, and BT-Med+BT-TI groups respectively, as compared to the untreated test group. In addition, cytoprotective activity in adenocarcinoma human alveolar basal epithelial cells (A549) showed improved cell viability by 11.3% (at 25.5µg/mL), 82.7% (at 63.75µg/mL), and 113.9% (at 25.5µg/mL) in the UT-Med+BT-TI, BT-Med+UT-TI, and BT-Med+BT-TI groups respectively, as compared to the untreated test group. MG-63 cells were used for estimation of ALP activity, which was highly increased by 59.8% (at 0.1µg/mL), 269.7% (at 0.1µg/mL), and 105.7% (at 0.1µg/mL) in the UT-Med+BT-TI, BT-Med+UT-TI, and BT-Med+BT-TI groups respectively, as compared to the untreated test group. Besides, Ishikawa cells showed maximum increased ALP activity by 94.8% at 10µg/mL in the BT-Med+UT-TI group as compared to the untreated group. The maximum percent cellular protection of HCF (heart) cells (decreased of LDH activity) was significantly increased by 48.8% (at 1µg/mL), 62.9% (at 10µg/mL), and 103% (at 1µg/mL) in the UT-Med+BT-TI, BT-Med+UT-TI group, and BT-Med+BT-TI groups respectively, as compared to the untreated test group. Alanine Amino Transferase (ALT) in terms of percent protection of HepG2 (liver) cells (decreased of ALT activity) was reported at 63.75µg/mL by 74.9% and 84.9% in the BT-Med+UT-TI and BT-Med+BT-TI groups respectively, as compared to the untreated test group. Cellular protection of A549 (lungs) cells (increased of SOD activity) in terms of percentage was increased by 21.7% (at 25.5µg/mL), 83.2% (at 0.1µg/mL), and 40% (at 25.5µg/mL) in the UT-Med+BT-TI, BT-Med+UT-TI, and BT-Med+BT-TI groups, respectively as compared to untreated group. Serotonin level was significantly increased by 50.8% (at 63.75µg/mL), 35.9% (at 10µg/mL), and 49.9% (at 10µg/mL) in the UT-Med+BT-TI, BT-Med+UT-TI, and BT-Med+BT-TI groups, respectively compared to untreated test group in human neuroblastoma cells (SH-SY5Y). However, the Relative Quantification (RQ) of Vitamin D Receptor (VDR) was significantly increased by 135.2%, 291.4%, and 248.3% at 50µg/mL in the UT-Med+BT-TI, BT-Med+UT-TI, and BT-Med+BT-TI groups, respectively as compared to the untreated in MG-63 cells. Thus, Biofield Energy Treated test formulation (The Trivedi Effect®) would be significantly useful for multiple organ health that can be used against coronary artery disease, arrhythmias, congenital heart disease, cardiomyopathy, cirrhosis, liver cancer, hemochromatosis, asthma, chronic bronchitis, cystic fibrosis, osteoporosis, etc.

Cholecalciferol is a steroid hormone (7-dehydroxycholesterol), which helps in the absorption of dietary minerals like zinc, calcium, magnesium, iron, and phosphate and also responsible for other biological activity. In this research study, the influence of the Trivedi Effect®-Consciousness Energy Healing Treatment on the physicochemical and thermal properties of cholecalciferol was evaluated using the modern analytical technique. The cholecalciferol test sample was divided into two parts. One of the test samples was considered as a control sample, which did not receive the Biofield Energy Treatment; whereas, the other part was treated with the Consciousness Energy Healing Treatment remotely by a well-known Biofield Energy Healer, Gopal Nayak and termed as a treated sample. The particle size values in the treated cholecalciferol powder sample were significantly decreased by 78.94% (d10), 26.21% (d50), 22.01% (d90), and 29.04% {D (4,3)}; thus, the specific surface area of the treated cholecalciferol was significantly increased by 174.12% compared to the control sample. The XRD peak intensities and crystallite sizes of the treated cholecalciferol powder sample were significantly altered ranging from -44.37% to 370.49% and -74.48% to 91.52%, respectively; therefore, the average crystallite size of the treated cholecalciferol was significantly decreased by 36.21% compared to the control cholecalciferol. The latent heat of fusion of the treated cholecalciferol was significantly increased by 25.89% compared to the control cholecalciferol. The weight loss was decreased by 1.48%; whereas, the residue amount was significantly increased by 107.02% in the treated sample compared with the control sample. Thus, the Trivedi Effect®-Consciousness Energy Healing Treatment generated a new polymorphic form of cholecalciferol which might offer better solubility, absorption, bioavailability, and be thermally more stable compared with the control sample. Henceforth, the Consciousness Energy Healing Treated cholecalciferol would be more beneficial to maintain the overall quality of life and it would be more useful in designing novel nutraceutical/pharmaceutical formulations for the better therapeutic responses against deficiency of vitamin D, osteoporosis, rickets, cardiovascular diseases, diabetes mellitus, cancer, mental disorders, multiple sclerosis, etc.

Withania somnifera (Ashwagandha) root extract and their active metabolites possess a broad range of pharmacological activities. The current study aimed to explore the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) on the physical, spectroscopic, and thermal properties of ashwagandha root extract using PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Ashwagandha root extract was divided into two parts – one part was control without any and another part was treated with The Trivedi Effect® - Energy of Consciousness Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as The Trivedi Effect® treated sample. The PXRD analysis exhibited that both the treated and control samples were amorphous in nature. The particle sizes at d10, d50, and d90 values of the treated sample were significantly decreased by 12.23%, 10.97%, and 1.25%, respectively with respect to the control sample. The surface area analysis revealed that the surface area of the treated sample was significantly increased by 14.29% as compared to the control sample. The FT-IR analysis indicated the alteration of the force constant for the functional groups of the treated sample in comparison to the control sample. The UV-vis analysis revealed that the wavelength for the maximum absorbance of the control and treated samples were at 205.8 and 206.0 nm, respectively. The TGA analysis revealed that the total weight loss was decreased by 0.70% in the treated sample as compared to the control sample. This indicated that the treated sample was thermally more stable as compared to the control sample. The DSC analysis indicated that the melting point of the treated sample (190.48°C) was decreased by 4.91% than the control sample (203.45°C). The latent heat of fusion of the treated (4.94 J/g) sample was significantly decreased by 42.96% compared with the control (8.66 J/g) sample. The current findings suggest that The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) might have the astounding capacity to enhance the solubility, absorption, dissolution, and finally, bioavailability of ashwagandha root extract in various form of pharmaceutical and nutraceutical formulation by modifying its particle size and surface area. Thus, the treated ashwagandha root extract might provide better therapeutic response against against inflammatory diseases, immunological disorders, stress, arthritis, cancer, diabetes, sexual disorders, aging and other chronic infections.

Sodium selenate is used for the prevention and treatment of various disorders like cancer, diabetes, inflammation diseases, etc. The objective of the current study was to evaluate the effect of The Trivedi Effect®-Energy of Consciousness Healing Treatment (Biofield Energy Treatment) on physicochemical, spectral, and thermal properties of The Trivedi Effect®-Biofield Energy Treated sodium selenate using various analytical methods such as PXRD, PSD, FT-IR, UV-vis spectroscopy, TGA, and DSC analysis. Sodium selenate was divided into two parts – one part was control without any treatment and another part was treated with The Trivedi Effect®-Biofield Energy Healing Treatment remotely by eighteen renowned Biofield Energy Healers and defined as The Trivedi Effect®-Energy of Consciousness Healing Treated sample. The PXRD analysis showed the significant alteration of the crystallite size of the treated sample in the range of -36.36% to 133.24% as compared to the control sample. However, the average crystallite size of the treated sample was significantly decreased by 8.31% as compared to the control sample. Overall, the crystal morphology of the treated sample was different from the control sample. The particle sizes d10, d50, and d90 values of the treated sample were significantly decreased by 3.40%, 9.75%, and 8.52%, respectively as compared to the control sample. Consequently, the surface area of the treated sample was significantly increased by 5.26% from the control sample. Both the control and treated FT-IR spectra indicated the presence of sharp and strong absorption bands at 887 cm-1 due to the Se=O stretching. The UV-vis spectroscopic analysis displayed that the wavelength for the maximum absorbance of both the control and treated samples were at 204.1 and 204.0 nm, respectively. TGA analysis revealed that the total weight loss of the treated sample was reduced by 1.24% as compared with the control sample. The DSC analysis showed that the treated sample (588.77°C) had higher melting point than the control sample (588.60°C). The latent heat of fusion was reduced in the treated sample by 8.01% compared to the control sample. Thus, The Trivedi Effect®-Biofield Energy Healing might lead to generate a new polymorphic (enantiotropic) form of sodium selenate which would be more soluble, bioavailable, and thermally stable compared with the untreated sample. The Trivedi Effect treated sodium selenate would be very useful to design better nutraceutical and/or pharmaceutical formulations that might offer better therapeutic response against inflammatory diseases, immunological disorders, stress, aging, infectious diseases, cancer, diabetes, heart diseases, Alzheimer’s disease, etc.

The current research work was aimed to investigate the impact of The Trivedi Effect® - Energy of Consciousness Healing Treatment (Biofield Energy Healing) on the structural properties and isotopic abundance ratio (PM+1/PM) of magnesium gluconate using LC-MS and NMR spectroscopy. Magnesium gluconate was divided into two parts. One part was denoted as the control, while the another part was defined as The Trivedi Effect® Treated sample, which received the Biofield Energy Healing Treatment remotely from eighteen renowned Biofield Energy Healers. The total ion chromatogram of the control sample showed two peaks at Rt of 1.81 and 2.06 min, whereas the treated sample displayed peaks at Rt of 1.79 and 2.04 min. The ESI-MS spectra of the control and the treated samples revealed the presence of the mass for magnesium gluconate ion in two forms at m/z 447 (adduct form with methanol) and 415 (protonated ion) in positive ionization mode. But, it showed the mass for the gluconate ion at m/z 195 in the negative ionization mode. The fragmentation pattern of magnesium gluconate in the treated sample was notably altered compared with the control sample. The proton and carbon signals for CH, CH2 and CO groups in the proton and carbon NMR spectra were found almost similar for the control and treated samples. The LC-MS based isotopic abundance ratio analysis indicated that the PM+1/PM (2H/1H or 13C/12C or 17O/16O or 25Mg/24Mg) in the treated magnesium gluconate ion at m/z 415 was significantly decreased by 79.24% compared with the control sample. Similarly, the isotopic abundance ratio of PM+1/PM (2H/1H or 13C/12C or 17O/16O) in the gluconate ion was decreased by 6.62% in the treated sample compared with the control sample. The treated magnesium gluconate might be beneficial in the nutraceutical and/or pharmaceutical industries for designing various forms of formulations, which could be providing better therapeutic response against various diseases such as diabetes mellitus, allergy, aging, inflammatory diseases, immunological disorders, and other chronic infections. Consequently, The Trivedi Effect® - Energy of Consciousness Healing Treated magnesium gluconate would be more helpful for understanding the enzymatic reactions as well as assist in the designing of the novel potent enzyme inhibitors by applying its kinetic isotope effects.

1-Chloro-3-nitrobenzene (3-CNB) is an aromatic halo-amine compound used as chemical intermediate for the production of several fine chemicals like pharmaceuticals, dyes, agricultural chemicals, etc. The stable isotope ratio analysis has drawn attention in numerous fields such as agricultural, food authenticity, biochemistry, etc. The objective of the current research was to investigate the impact of the biofield energy treatment on the isotopic abundance ratios of PM+1/PM, PM+2/PM and PM+3/PM in 3-CNB using gas chromatography - mass spectrometry (GC-MS). The sample, 3-CNB was divided into two parts - one part was denoted as control and another part was referred as biofield energy treated sample that was treated with biofield energy (The Trivedi Effect®). T1, T2, T3, and T4 were represented to different time interval analysis of the biofield treated 3-CNB. The GC-MS spectra of the both control and biofield treated 3-CNB indicated the presence of molecular ion peak [M+] at m/z 157 (calculated 156.99 for C6H4ClNO2) along with same pattern of fragmentation. The relative intensities of the parent molecule and other fragmented ions of the biofield treated 3-CNB were improved as compared to the control 3-CNB. The percentage change of the isotopic abundance ratio of PM+1/PM was significantly increased in the biofield treated 3-CNB at T1, T2 and T3 by 11.62, 18.50, and 29.82%, respectively with respect to the control 3-CNB. Accordingly, the isotopic abundance ratio of PM+2/PM in the biofield treated 3-CNB at T2 and T3 was significantly improved by 15.22 and 35.09%, respectively as compared to the control sample. The isotopic abundance ratios of PM+1/PM and PM+2/PM in the biofield treated 3-CNB at T1 and T4 were changed as compared to the control sample. The percentage change of the isotopic abundance ratio of PM+3/PM was enhanced in the biofield treated 3-CNB at T1, T2, T3, and T4 by 4.67, 18.69, 31.31 and 6.08%, respectively as compared to the control 3-CNB. The isotopic abundance ratios of PM+1/PM, PM+2/PM and PM+3/PM in the biofield treated 3-CNB changed with the time. So, the biofield energy treated 3-CNB might exhibit the altered isotope effects such as altered physicochemical and thermal properties, binding energy, and the rate of the chemical reaction as compared to the control sample. The biofield energy treated 3-CNB might assist in designing for the synthesis of pharmaceuticals, agricultural chemicals, dyes, corrosion inhibitors and other several useful industrial chemicals.

Nitrophenols are the synthetic organic chemicals used for the preparation of synthetic intermediates, organophosphorus pesticides, and pharmaceuticals. The objective of the present study was to evaluate the effect of biofield energy treatment on the isotopic abundance ratios of PM+1/PM, and PM+2/PM in o- and m-nitrophenol using the gas chromatography-mass spectrometry. The o- and m-nitrophenol were divided into two parts - one part was control sample, and another part was considered as biofield energy treated sample, which received Mr. Trivedi’s biofield energy treatment (The Trivedi Effect®). The biofield energy treated nitrophenols having analyzed at different time intervals were designated as T1, T2, T3, and T4. The GC-MS analysis of both the control and biofield treated samples indicated the presence of the parent molecular ion peak of o- and m-nitrophenol (C6H5NO3+) at m/z 139 along with major fragmentation peaks at m/z 122, 109, 93, 81, 65, and 39. The relative peak intensities of the fragmented ions in the biofield treated o- and m-nitrophenol were notably changed as compared to the control sample with respect to the time. The isotopic abundance ratio analysis using GC-MS revealed that the isotopic abundance ratio of PM+1/PM in the biofield energy treated o-nitrophenol at T2 and T3 was significantly increased by 14.48 and 86.49%, respectively as compared to the control sample. Consequently, the isotopic abundance ratio of PM+2/PM in the biofield energy treated sample at T2 and T3 was increased by 11.36, and 82.95%, respectively as compared to the control sample. Similarly, in m-nitrophenol, the isotopic abundance ratio of PM+1/PM in the biofield energy treated sample at T1, T3, and T4 was increased by 5.82, 5.09, and 6.40%, respectively as compared to the control sample. Subsequently, the isotopic abundance ratio of PM+2/PM at T1, T2, T3 and T4 in the biofield energy treated m-nitrophenol was increased by 6.33, 3.80, 16.46, and 16.46%, respectively as compared to the control sample. Overall, the isotopic abundance ratios of PM+1/PM (2H/1H or 13C/12C or 15N/14N or 17O/16O), and PM+2/PM (18O/16O) were altered in the biofield energy treated o- and m-nitrophenol as compared to the control increased in most of the cases. The biofield treated o- and m-nitrophenol that have improved isotopic abundance ratios might have altered the physicochemical properties and could be useful in pharmaceutical and chemical industries as an intermediate in the manufacturing of pharmaceuticals and other useful chemicals for the industrial application.

Bone formation, remodeling and healing processes involves a coordinated action of different cell types. Vitamin D and calcium significantly improves the patients' quality of life with respect to various bone disorders and comorbidities most prevalent in the elderly. The current study aimed to evaluate the potential of Consciousness Energy Healing based vitamin D3 and DMEM medium on bone health. Vitamin D3 and DMEM medium as test items (TI), were divided into two parts. One part of each sample received the Consciousness Energy Healing Treatment by Vicki Lynn Kindy and those samples were labeled as the Biofield Energy Treated (BT) samples, while the other parts of each sample were denoted as the untreated test items (UT). Bone health biomarkers such as alkaline phosphatase enzyme (ALP) activity, collagen levels and bone mineralization were evaluated. Cell viability using 3-(4, 5-diamethyl-2-thiazolyl)-2, 5-diphenyl-2H-tetrazolium) (MTT) data showed that the test items were found to be safe. ALP level was significantly increased by 621.2%, 638.9%, and 102.2% at 1, 10, and 50 µg/mL, respectively in UT-DMEM+BT-TI, as compared to the untreated group. Collagen content was significantly increased up to 284.6%, 134%, and 183.4% at 1, 10, and 50 µg/mL, respectively in the UT-DMEM+BT-TI group, while 109.6% and 159.6% increased collagen in BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 1 µg/mL as compared with the untreated group. Moreover, the percent of bone mineralization was significantly increased by 120% and 229.4% at 50 and 100 µg/mL, respectively in UT-DMEM+BT-TI group, while 254.7% and 78.1% in BT-DMEM+UT-TI and BT-DMEM+BT-TI groups, respectively at 10 µg/mL as compared with the untreated group. Thus, Biofield Energy Treated vitamin D3 and DMEM would play an important role to control the osteoblast function, improves bone mineralization, and calcium absorption in many bone disorders. Moreover, the bone health parameters such as collagen, calcium and ALP were significantly improved and can be used as supplement to improve bone health. Based on the outstanding results, it is assumed that the Biofield Energy Treated vitamin D3 could be a powerful alternative dietary sources and supplements to fight against various bone related diseases including low bone density and osteoporosis, osteogenesis imperfecta, Paget’s disease of bone, rickets, osteomalacia, bone and/or joint pain, increased frequency of fractures, deformed bones, osteoma, chondrodystrophia fetalis, hormonal imbalance, stress, aging, bone loss and fractures.

A new proprietary herbomineral formulation was formulated, consisting of essential ingredients viz. herbal root extract ashwagandha and minerals (zinc, magnesium, and selenium). The aim of the study was to evaluate the immunomodulatory potential of Consciousness Energy Healing Treatment on the herbomineral formulation in male Sprague Dawley rats. The test formulation was divided into two parts. One part was denoted as the control without any Biofield Energy Treatment, while the other part was defined as the Biofield Energy Treated sample, which received the Biofield Energy Healing Treatment remotely from seven renowned Biofield Energy Healers. Additionally, one group of animals also received Biofield Energy Treatment per se (day -15) by Biofield Energy Healers under similar conditions. The test formulation was evaluated for immunological parameters viz. humoral immune analysis, paw volume, hematological study, biochemistry, body weight, feed and water intake, and histopathology analysis were performed. The humoral immune response showed a significant increased primary and secondary antibody titre values by 26% and 150.4%, respectively in Biofield Energy Treatment per se group (G6, day -15), while primary and secondary antibody titre values were increased by 100.0% and 110.4%, respectively in the Biofield Energy Treated test formulation (G7, day -15) group compared with the disease control group (G2). The results of delayed type hypersensitivity showed a significant increase in the paw volume by 94.44% in the Biofield Energy Treated test formulation (G4) group with respect to the G2 group. However, a significant increase in paw volume by 61.11% and 44.44% was found in G6 and G7 group, respectively with respect to the G2 group. Hematological parameters showed a significant (p<0.001) increased platelet count by 85.6% in the G4 group, while 39.15% and 35.22% increase in the G6 and G7 groups, respectively compared with the G2 group. In biochemical analysis, a significant increase in calcium and phosphorus level were found in the G4 group compared with the G2 group. Animal weight parameters suggests that there were no treatment-related changes in any group, organ to body weight ratio, feed and water intake. The data described that the Biofield Energy Treated test formulation was found to be safe without any side effect during the course of the experiment. These data suggests that the Biofield Energy Treated test formulation and Biofield Energy Treatment per se can be used for autoimmune and inflammatory diseases, enhancing stress management and prevention, and anti-aging by improving overall health.

The study aim was to investigate the anti-stress effect of Consciousness Energy Healing Treated test formulation using behavioral tests, hormonal levels and antioxidants in male Sprague Dawley rats. The test formulation was divided into two parts, one was represented as control, while the other was treated with Biofield Energy by Mr. Mahendra Kumar Trivedi (The Trivedi Effect® - Consciousness Energy Healing) and defined as the Biofield Energy Treated sample. OFT data showed the frequency of total square-cross was significantly (p ≤ 0.05) increased by 49.11% in the animals per se received Biofield Energy Treatment (-15 days) (G6) compared to stress control (G2). Freezing time was significantly (p ≤ 0.01) reduced by 21.48%, 43.79%, 42.79%, 44.79%, and 58.21% in the Biofield Energy Treated test formulation (G5), G6, Biofield Energy Treated test formulation (-15 days) (G7), Biofield Energy Treated animals with Biofield Energy Treated test formulation (-15 days) (G8) and Biofield Energy Treatment per se to animals plus untreated test formulation (G9), respectively compared to G2. Defecation was significantly (p ≤ 0.05) decreased by 92.01% in the G8 compared to G2. Urination was significantly reduced by 53.19% and 46.81% in the G8 and G9, respectively compared to G2. FST showed the number of climbing was significantly (p ≤ 0.05) increased by 37.5%, 70.83%, 50%, 102.17%, and 68.83% in the G5, G6, G7, G8, and G9, respectively compared to G2. Swimming time was significantly (p ≤ 0.05) increased by 30.09%, 70%, 17.66%, 83%, and 100% in the G5, G6, G7, G8, and G9, respectively compared to G2. The resting time was significantly (p ≤ 0.05) decreased by 21.54%, 50%, 55%, and 63% in the G5, G6, G8, and G9, respectively compared to G2. Corticosterone was significantly decreased by 29%, 26%, 31%, and 36% in the G5, G6, G8, and G9, respectively compared to G2. Testosterone was significantly increased by 275% (p ≤ 0.05), 83%, 70%, 181%, and 164% in the G5, G6, G7, G8, and G9, respectively compared to G2. SOD was significantly (p ≤ 0.001) increased by 15%, 53%, 57%, 61%, and 58% in the G5, G6, G7, G8, and G9, respectively compared to G2. Further, CAT was significantly increased by 19% and 14% in the G5 and G6, respectively compared to G2. Overall, data suggest that Biofield Energy Treatment per se and Biofield Energy Treated test formulation have significant anti-stress activity and could be utilized in various stress related disorders like asthma, obesity, diabetes, headaches, depression and anxiety, gastrointestinal problems, Alzheimer’s disease, accelerated aging, and premature death.

Magnesium (Mg) is abundantly present in the human body, which is important for many metabolic activities, and normal functioning of cells, muscles, heart, bones, etc. The objective of this study was to evaluate the impact of the Trivedi Effect® on the physicochemical properties of Mg powder using PXRD and PSA analytical techniques. Mg powder sample was divided into two parts, one part of Mg was considered as control (no Biofield Energy Treatment was provided), while the second part received the Consciousness Energy Healing Treatment remotely by a renowned Biofield Energy Healer, Gopal Nayak and termed as a Consciousness Energy Healing Treated sample. The PXRD peak intensities and crystallite sizes of the treated Mg were significantly altered ranging from 2.86% to 16.51% and 6.79% to 52.30%, respectively compared to the control sample. Overall the average crystallite size of the treated Mg sample was significantly increased by 16.92% compared with the control sample. The particle size values in the treated Mg were significantly decreased by 5.5% (d10), 7.26% (d50), 8.63% (d90), and 7.69% {D(4,3)} compared to the control sample. The specific surface area of Consciousness Energy Healing Treated Mg powder (0.0187m2/g) was significantly increased by 7.47% compared with the control sample (0.0174m2/g). The Trivedi Effect® might lead to generate a new polymorphic form of Mg which would show better solubility in solvents compared with the control sample. The Consciousness Energy Healing Treated Mg would be very useful for the prevention and treatment of many diseases such as hypomagnesaemia, ventricular arrhythmias and the prevention of sudden cardiac death, migraine, inflammatory diseases, immunological disorders, pre-eclampsia, eclampsia, diabetes mellitus, cancer, gestational hypertension, oxidative stress, etc. It would be more beneficial for the designing of nutraceutical/pharmaceutical formulations, and other metal and chemical industries.

Silver sulfadiazine is a topical medicine that belongs to sulfa antibiotics class of drugs and used in treating wound infections in burn patients. The aim of this study was to determine the effect of Consciousness Energy Healing Treatment (the Trivedi Effect®) on the various properties of silver sulfadiazine with the help of modern analytical techniques. The sample was divided into two parts; the first part was not given any treatment and considered as a control sample, while the second part was provided the Consciousness Energy Healing Treatment by the Biofield Energy Healer, Gopal Nayak remotely, named as the treated sample. The powder XRD data showed significant alterations in the peak intensities of the treated sample ranging from-30.71% to 47.54% compared to the control sample. The crystallite size was altered ranging from -78.12% to 1.47%; and the average crystallite size was significantly reduced by 31.62% in the treated sample compared to the control sample. The particle sizes were decreased in the treated sample by 12.75%(d10), 4.98%(d50), 0.89%(d90), and 2.92%{D(4,3)}; thus, the specific surface area was significantly increased by 17.31% compared with the control sample. The latent heat of fusion and latent heat of decomposition were profoundly increased by 24.62% and 156.28%, respectively in the treated sample compared to the control sample. The total weight loss was increased by 3.08% and the residue amount was reduced by 4.44% in the treated sample compared to the control sample. Thus, the Trivedi Effect®-Consciousness Energy Healing Treated sample might form a new polymorph of silver sulfadiazine that possesses reduced particle size and improved thermal properties compared to the untreated sample. Therefore, the Biofield Energy treated sample can be used in nutraceutical/pharmaceutical formulation, which would show a better bioavailability and therapeutic response against various infections in comparison to the control sample.

The wound healing activity using scratch assay is considered as a convenient in vitro tool for the assessment of wound healing. The present study deals with the optimization of Biofield Energy Treatment (Consciousness Energy Healing Treatment-The Trivedi Effect®) in the HFF-1 cell line (Human Foreskin Fibroblast) and DMEM (Dulbecco’s Modified Eagle Medium) using scratch assay against positive control, recombinant Human Epidermal Growth Factor (Hu EGF, 30 ng/mL). This method was used for the determination of cell proliferation and migration of fibroblast quantitatively in the scratched wounded area. The scratched area was monitored after 24 hours of wound closure in the Biofield Energy Treated HFF-1 cells and the Biofield Energy Treated DMEM groups, and the representative photomicrographs were taken in each wells using Wim Scratch Image analysis software. The results showed that the Biofield Energy Treated DMEM was significantly higher percentage of fibroblast migration i.e. 51.8%, while the migration was altered in the Biofield Energy Treated HFF-1 cell line compared to the baseline control group. In addition to, the percentage of scratch area was significantly decreased by 2.7% in the Biofield Energy Treated DMEM group, while it was increased by 12% in the Biofield Treated cells group compared to the baseline control group. Overall, the experimental results concluded that The Trivedi Effect® has the significant capacity and wide implications in wound healing activity via cell culture media, DMEM as compared with the HFF-1 cell line directly. Biofield Energy Healing would be a complementary and alternative medicine that can be used against burn injury cases, acute wound, skin regeneration, eczema, diaper rash, chickenpox, measles, warts, acne, hives, wrinkles, ringworm, Rosacea, psoriasis, seborrheic dermatitis, skin cancer, etc.