Search Results (5,612)

Laboratory mice are instrumental for preclinical research but there are serious concerns that the use of a clean standardized environment for specific-pathogen-free (SPF) mice results in poor bench-to-bedside translation due to their immature immune system. The aim of the present study was to test the importance of the gut microbiota in wild vs. SPF mice for evaluating host immune responses in a house-dust-mite-induced allergic airway inflammation model without the influence of pathogens. The wild mouse microbiome reduced histopathological changes and TNF-α in the lungs and serum when transplanted to microbiota-depleted mice compared to mice transplanted with the microbiome from SPF mice. Moreover, the colonic gene expression of Gata3 was significantly lower in the wild microbiome-associated mice, whereas Muc1 was more highly expressed in both the ileum and colon. Intestinal microbiome and metabolomic analyses revealed distinct profiles associated with the wild-derived microbiome. The wild-mouse microbiome thus partly reduced sensitivity to house-dust-mite-induced allergic airway inflammation compared to the SPF mouse microbiome, and preclinical studies using this model should consider using both ‘dirty’ rewilded and SPF mice for testing new therapeutic compounds due to the significant effects of their respective microbiomes and derived metabolites on host immune responses. Full article

This study addresses FinTech implementation challenges in the banking industry in Palestine. This was accomplished by adopting qualitative research methods. Semi-structured interviews were conducted with interviewees from the Palestinian Monetary Authority, banks, and FinTech companies. Thematic analysis was conducted using NVivo 12 software to identify themes in the interview scripts. Research outcomes suggest that FinTech development in Palestine encounters a range of multifaceted challenges, which can be categorised using the TOE (technological, organisational, environmental) framework. On the technological front, issues such as underdeveloped IT and telecommunications infrastructure, restricted mobile frequencies due to Israeli occupation, limited IT expertise, cyber risks, low digital literacy, and minimal FinTech awareness hinder progress. Organizationally, resistance to change, inadequate agility, limited digital skills, and slow Sharia compliance updates in Islamic banking impede innovation. Environmentally, the absence of a dedicated FinTech framework, unclear regulatory guidance, limited market size, and strict AML/CFT regulations create uncertainties for non-bank entities and restrict investment opportunities. Addressing these interconnected barriers requires coordinated efforts across legal, financial, and technological sectors to foster FinTech integration and growth in Palestine. Full article

Gravity may exert species-specific effects on quadrupedal vasoreactivity, reflecting variations in the vertical displacement of the cardiocranial axis from the dorsal plane. Deer show markedly displaced cardiocranial axes compared to their closest phylogenetic relatives, but their relative cerebrovascular responses remain unelucidated. Accordingly, we investigated the responses to noradrenaline (NA), acetylcholine (ACh), 5-hydroxytryptamine (5-HT), histamine, angiotensin (Ang) II, and bradykinin (BK) in cervine basilar arterial rings. NA and 5-HT induced slight contraction, and ACh induced relaxation, which contrasts with the findings reported in pigs and cattle. The cumulative response to ACh was abolished by endothelial denudation and inhibited by N^ω-nitro-L-arginine (a nitric oxide synthase inhibitor), atropine (a nonselective muscarinic antagonist), and p-fluoro-hexahydro-sila-difenidol (an M₃ antagonist). Pirenzepine (an M₁ antagonist) and methoctramine (an M₂ antagonist) showed no significant effects. Histamine induced contractions, with its concentration–response curve shifted to the right in parallel by diphenhydramine (an H₁ antagonist). However, cimetidine (an H₂ antagonist) showed no significant effects. Ang II and BK had no vasomotive effects. NA and ACh induced different cerebrovascular responses in sika deer versus cattle, but histamine and BK did not. Our findings suggest that cerebrovascular responses are influenced by the similarity of animal species and the head and heart positions relative to gravity. Full article

Background: Solid tumors vary by the immunogenic potential of the tumor microenvironment (TME) and the likelihood of response to immunotherapy. The emerging literature has identified key immune cell populations that significantly impact immune activation or suppression within the TME. This study investigated candidate T-cell populations and their differential infiltration within different tumor types as estimated from mRNA co-expression levels of the corresponding cellular markers. Methods: We analyzed the mRNA co-expression levels of cellular biomarkers that define stem-like tumor-infiltrating lymphocytes (TILs), tissue-resident memory T-cells (TRM), early dysfunctional T-cells, late dysfunctional T-cells, activated-potentially anti-tumor (APA) T-cells and Butyrophilin 3A (BTN3A) isoforms, utilizing clinical and transcriptomic data from 1892 patients diagnosed with melanoma, bladder, ovarian, or pancreatic carcinomas. Real-world data were collected under the Total Cancer Care Protocol and the Avatar^® project (NCT03977402) across 18 cancer centers. Furthermore, we compared the survival outcomes following immune checkpoint inhibitors (ICIs) based on immune cell gene expression. Results: In melanoma and bladder cancer, the estimated infiltration of APA T-cells differed significantly (p = 4.67 × 10⁻¹² and p = 5.80 × 10⁻¹², respectively) compared to ovarian and pancreatic cancers. Ovarian cancer had lower TRM T-cell infiltration than melanoma, bladder, and pancreatic (p = 2.23 × 10⁻⁸, 3.86 × 10⁻²⁸, and 7.85 × 10⁻⁹, respectively). Similar trends were noted with stem-like, early, and late dysfunctional T-cells. Melanoma and ovarian expressed BTN3A isoforms more than other malignancies. Higher densities of stem-like TILs; TRM, early and late dysfunctional T-cells; APA T-cells; and BTN3A isoforms were associated with increased survival in melanoma (p = 0.0075, 0.00059, 0.013, 0.005, 0.0016, and 0.041, respectively). The TRM gene signature was a moderate predictor of survival in the melanoma cohort (AUROC = 0.65), with similar findings in testing independent public datasets of ICI-treated patients with melanoma (AUROC 0.61–0.64). Conclusions: Key cellular elements related to immune activation are more heavily infiltrated within ICI-responsive versus non-responsive malignancies, supporting a central role in anti-tumor immunity. In melanoma patients treated with ICIs, higher densities of stem-like TILs, TRM T-cells, early dysfunctional T-cells, late dysfunctional T-cells, APA T-cells, and BTN3A isoforms were associated with improved survival. Full article

Human-induced climate change has highlighted the need for community-based adaptation (CBA) to build resilience in vulnerable communities. CBA empowers communities to leverage their resources and skills in shaping effective adaptation strategies. Agent-based modelling (ABM) is a suitable tool to develop tailored strategies that account for local capacities, priorities, and cultural contexts. This study assesses ABM tools for their suitability to model CBA, focusing on key criteria such as agent definition, sensitivity analysis, scalability, and experiment design. A comprehensive review of available ABM tools identifies NetLogo as the most fitting tool by its features, due to its flexibility in handling complex community–environment interactions. GAMA and Envision are nearly as suitable, offering robust support for modelling socio-economic and environmental dynamics. This article provides guidance for researchers and practitioners in choosing an appropriate ABM tool aligning with the specific needs of CBA, ensuring contextually relevant and sustainable adaptation solutions. Full article

This article explores the historical and contemporary relationships between Jews and Muslims, with a focus on the implications for Islamic religious education. It highlights the complexity of Jewish–Muslim relations, characterized by periods of coexistence and conflict. Early Islamic history reveals an inclusive community of believers encompassing pious Jews and Christians, challenging modern perceptions of inherent antagonism. The article examines the evolution of these relationships, noting significant changes influenced by political dynamics. In Germany, political discourses further shape mutual perceptions, often exacerbating tensions between Jewish and Muslim communities. Educationally, the article advocates for addressing distorted perspectives and emphasizes the value of intertextual analysis of the Quran and the Torah. Full article

Ibuprofen is a significant nonsteroidal anti-inflammatory drug that poses environmental and health risks when present in wastewater because of its persistence and probable toxicity. This study investigates the use of banana peel biochar (BPB) made at 600 °C to 900 °C to eliminate ibuprofen from aqueous solutions. The uniqueness of this work lies in the high-temperature pyrolysis process, which has not been previously explored for the ibuprofen removal efficiency using BPB. The batch experiment was conducted considering initial concentrations, pH, and contact time. The data were compared with different algorithms, with Linear Regression (LR), Support Vector Machines (SVM), Decision Trees (DT), Random Forest (RF), and k-Nearest Neighbor (k-NN) to forecast the performance. The results revealed that banana peel biochar at 900 °C exhibited the highest ibuprofen removal efficiency (69.28 ± 0.83%) at 125 mg/L concentration with the sequence of BPB900 > BPB800 > BPB700 > BPB600. A maximum removal efficiency of 72.67 ± 0.75% was observed at pH 9. Adsorption behavior was analyzed using isotherm and kinetic models, with the Freundlich isotherm model (R² value 0.9620) indicating heterogeneous adsorption and the pseudo-second-order (PSO) kinetic model (R² value 0.9969) suggesting that physicochemical interactions govern the process. FTIR analysis ensured the existence of functional groups (hydroxyl, carboxylic, carbonyl, and aromatic rings) responsible for adsorption. Machine learning algorithms, especially RF, demonstrated outstanding performance with 90.07% accuracy in predicting the experimental data. In comparison to other adsorbents, BPB demonstrated superior removal efficiency, underscoring its effectiveness. The study suggests that BPB, particularly at 900 °C, is effective in removing ibuprofen, and due to its sustainable production, it offers a potential solution for wastewater treatment. Full article

The demand for developing novel antimicrobial drugs has increased due to the rapid appearance and global spread of antibiotic resistance. Antimicrobial peptides (AMPs) offer distinct advantages over traditional antibiotics, such as broad-range efficacy, a delayed evolution of resistance, and the capacity to enhance human immunity. AMPs are being developed as potential medicines, and current computational and experimental tools aim to facilitate their preclinical and clinical development. Structural and functional constraints as well as a more stringent regulatory framework have impeded clinical translation of AMPs as possible therapeutic agents. Although around four thousand AMPs have been identified so far, there are some limitations of using these AMPs in clinical trials due to their safety in the host and sometimes limitations in the biosynthesis or chemical synthesis of some AMPs. Overcoming these obstacles may help to open a new era of AMPs to combat superbugs without using synthetic antibiotics. This review describes the classification, mechanisms of action and immune modulation, advantages, difficulties, and opportunities of using AMPs against multidrug-resistant pathogens and highlights the need and priorities for creating targeted development strategies that take into account the most cutting-edge tools currently available. It also describes the barriers to using these AMPs in clinical trials. Full article

Bacillus sp. THPS1 is a novel strain isolated from a high-temperature hot spring in Thailand, exhibiting distinctive genomic features that enable adaptation to an extreme environment. This study aimed to characterize the genomic and functional attributes of Bacillus sp. THPS1 to understand its adaptation strategies and evaluate its potential for biotechnological applications. The draft genome is 5.38 Mbp with a GC content of 35.67%, encoding 5606 genes, including those linked to stress response and sporulation, which are essential for survival in high-temperature conditions. Phylogenetic analysis and average nucleotide identity (ANI) values confirmed its classification as a distinct species within the Bacillus genus. Pangenome analysis involving 19 others closely related thermophilic Bacillus species identified 1888 singleton genes associated with heat resistance, sporulation, and specialized metabolism, suggesting adaptation to nutrient-deficient, high-temperature environments. Genomic analysis revealed 12 biosynthetic gene clusters (BGCs), including those for polyketides and non-ribosomal peptides, highlighting its potential for synthesizing secondary metabolites that may facilitate its adaptation. Additionally, the presence of three Siphoviridae phage regions and 96 mobile genetic elements (MGEs) suggests significant genomic plasticity, whereas the existence of five CRISPR arrays implies an advanced defense mechanism against phage infections, contributing to genomic stability. The distinctive genomic features and functional capacities of Bacillus sp. THPS1 make it a promising candidate for biotechnological applications, particularly in the production of heat-stable enzymes and the development of resilient bioformulations. Full article

This paper reexamines climate justice through the framework of environmental sociology, offering fresh perspectives on the intersection of social and ecological systems in the face of escalating global climate crises. It emphasizes that inequality lies at the heart of global climate politics, often obstructing pathways toward achieving a true climate solution. Drawing from established traditions within environmental sociology—such as the new ecological paradigm, the post-growth society, and the environmental justice paradigm—the paper advocates for profound systemic and structural reforms in political and economic systems to tackle entrenched inequalities. By integrating these frameworks, the paper proposes a comprehensive model of climate justice, encompassing material, procedural, compensatory, and transformative dimensions of justice. This holistic approach not only addresses environmental sustainability but also prioritizes social equity, ensuring that marginalized communities are included in the global response to climate change. The paper thus positions this model as a critical component of broader environmental and social transformation. Full article

The snap bean (Phaseolus vulgaris L.) is highly sensitive to both phosphorus (P) deficiency and hypoxic stress, which together can significantly hinder plant growth, nutrient uptake, and yield; however, limited information exists on the effect of P and oxygen (O₂) fertilization to alleviate these stresses and enhance yield. A two-year field experiment assessed the effects of P and O₂ fertilization on plant growth, pod yield, and P uptake in acidic sandy soil. Using a randomized complete block design with four replications, we tested five P rates (0, 45, 90, 135, and 179 kg ha⁻¹ of phosphorus pentoxide, P₂O₅) in the form of triple superphosphate (TSP) along with two rates (0 and 45 kg ha⁻¹) of solid O₂ fertilizer as calcium peroxide (CaO₂). Phosphorus and O₂ fertilizers improved plant growth and pod yield, with the highest yield from the combination of 135 kg ha⁻¹ P₂O₅ and 45 kg ha⁻¹ CaO₂. Pearson correlation analysis indicated strong associations between plant growth, pod yield, and nutrient accumulation. Principal component analysis (PCA) highlighted notable seasonal differences in snap bean and soil characteristics. This study provides essential insights into the use of O₂ fertilizers as a cost-effective approach to mitigate hypoxia, enhance P use efficiency, and improve yield in snap bean. Our findings may inspire the development of sustainable nutrient protocols for high-quality snap bean production and serve as a foundation for similar applications in other crops. Full article

This study aims to develop efficient and sustainable hydrogels for dye adsorption, addressing the critical need for improved wastewater treatment methods. Carboxymethyl cellulose (CMC)-based hydrogels grafted with AAc were synthesized using gamma radiation polymerization. Various AAc to CMC ratios (5:5, 5:7.5, 5:10, 5:15) were treated with 37% NaOH and exposed to 1–15 kGy radiation, with the optimal hydrogel obtained at 5 kGy. Swelling studies showed an increase in swelling with 5–7.5% AAc content, with the 5:7.5 hydrogel achieving the highest swelling at 18,774.60 (g/g). FTIR spectroscopy confirmed the interaction between AAc and CMC, indicating the successful formation of the hydrogel. DSC analysis revealed that higher AAc content led to increased glass transition and decomposition temperatures, thereby enhancing thermal stability. The swelling kinetics were linked to a reduction in pore size and improved AAc grafting. The 5:7.5 hydrogel demonstrated the highest adsorption capacity (681 mg/g) for methylene blue at 80 mg/L, achieving a desorption efficiency of 95% in 2M HCl. Kinetic analysis revealed non-uniform physisorption on a heterogeneous surface, which followed Schott’s pseudo-second-order model. This study advances the development of efficient hydrogels for water purification, providing a cost-effective and environmentally friendly solution for large-scale applications. Full article

This study examines how Ibn Arabi’s metaphysics can address key challenges in the contemporary philosophical debate on the problem of evil. John Mackie famously argues that the existence of an omnibenevolent and omnipotent God is logically incompatible with the existence of evil, suggesting that theism must relinquish one of these divine attributes to resolve this contradiction. Alvin Plantinga, through his Free Will Defense, demonstrates that no logical contradiction undermines the coherence of the theistic conception of God. Although Mackie concedes this point, he contends that Plantinga’s defense does not sufficiently explain why God permits evil. With the resolution of the logical problem of evil, the evidential problem has gained prominence in the theistic debate, where Plantinga’s defense remains inadequate. While Plantinga invites theists to explore potential theological reasons for God’s allowance of evil, he acknowledges that this approach may not yield strong philosophical results. In contrast, Ibn Arabi’s metaphysical framework offers a more comprehensive solution. By integrating ontology, epistemology, and metaphysics to establish a coherent relationship between God, the universe, and humanity, Ibn Arabi renders Mackie’s logical critique irrelevant. Furthermore, his approach fills the gaps left by Plantinga’s defense, offering a more nuanced understanding of the relationship between God and evil. Full article

This research article investigates the recycling of end-of-life solar photovoltaic (PV) panels by analyzing various mechanical methods, including Crushing, High Voltage Pulse Crushing, Electrostatic Separation, Hot Knife Cutting, Water Jet Cutting, and Magnetic Separation. Each method’s effectiveness in extracting materials such as glass, silicon, metals (copper, aluminum, silver, tin, lead), and EVA was evaluated. The analysis reveals that no single method is entirely sufficient for comprehensive material recovery. Based on the data analysis, a new hypothetical hybrid method, Laser and High Voltage Pulse (L&HVP), is proposed, which integrates the precision of laser irradiation with the robustness of high voltage pulse crushing. The laser irradiation step would theoretically facilitate the removal of the ethylene-vinyl acetate (EVA) encapsulant, preparing the materials for subsequent separation. The high high-voltage pulse crushing would then selectively fragment and separate the remaining components, potentially enhancing material recovery efficiency while minimizing contamination. The proposed approach is grounded in the observed limitations of existing techniques. This method aims to offer a more comprehensive and sustainable solution for solar PV module recycling. Further research and experimentation are necessary to validate the effectiveness of the L&HVP method and its potential impact on the field of solar PV recycling. Full article

This article explores the compatibility of liberal citizenship with Twelver Shia jurisprudence, a topic previously analyzed from the perspective of Sunni schools, most notably in the extensive research of Andrew F. March. This study confronts the challenges of reconciling liberal citizenship with Islamic jurisprudence, as highlighted in March’s work, through the lens of Shia legal thought. Rather than aiming to critique or review March’s research, this article considers his work solely as a representative example addressing the topic from the perspective of Sunni jurisprudence. This approach provides readers with a fundamental contrast, illuminating the unique insights that emerge from examining the subject within the framework of Shia jurisprudence. Unlike Sunni jurisprudence, which addresses these issues case-by-case by reviewing relevant Quranic and narrational sources, Twelver Shia jurisprudence offers a more foundational resolution. Owing to the belief in the occultation of the twelfth Imam and its implications for the implementation of Islamic law, Shia scholars have advanced theories such as the theory of obstruction (insidād) and the suspension of the social and political dimensions of Sharia. These theories effectively narrow the scope of Sharia, allowing for the acceptance of laws from non-Islamic states and circumventing potential conflicts with liberal citizenship in the absence of the twelfth Imam. Full article