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Search Results (497)

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21 pages, 10458 KiB  
Article
Response of Sediment Dynamics to Tropical Cyclones under Various Scenarios in the Jiangsu Coast
by Can Wang, Chengyi Zhao, Gang Yang, Chunhui Li, Jianting Zhu and Xiaofei Ma
J. Mar. Sci. Eng. 2024, 12(7), 1053; https://doi.org/10.3390/jmse12071053 - 23 Jun 2024
Viewed by 274
Abstract
The Jiangsu Coast (JC), China, is an area susceptible to the impact of tropical cyclones (TCs). However, due to the lack of available on-site observation data, nearshore sedimentary dynamic processes under the impact of TCs have not been fully explored. This study developed [...] Read more.
The Jiangsu Coast (JC), China, is an area susceptible to the impact of tropical cyclones (TCs). However, due to the lack of available on-site observation data, nearshore sedimentary dynamic processes under the impact of TCs have not been fully explored. This study developed a 3D wave–current–sediment numerical model for the JC based on the Finite Volume Community Ocean Model (FVCOM) to investigate sediment dynamic responses to TCs under various scenarios, including different tracks, intensities of TCs and tidal conditions. The validation results demonstrated the model’s satisfactory performance. According to the simulation results, typhoons can significantly impact the hydrodynamics and sediment dynamics. During Typhoon Lekima in 2019, strong southeasterly winds substantially increased the current velocity, bottom stress, wave height, and suspended sediment concentration (SSC). Three typical landfall-type typhoons, with prevailing southeasterly winds, brought significant sediment flux from southeast to northwest along the coast, while the typhoon that moved northward in the Yellow Sea induced a relatively small sediment flux from north to south. Typhoons could also induce stripe-like erosion and deposition, which is closely related to seafloor topography, resulting in seabed thickness variations of up to ±0.3 m. Additionally, strengthening typhoon wind fields can lead to increased sediment flux and seabed morphological changes. Typhoon Winnie, particularly at spring tide, had a greater impact on sediment dynamics compared to other landfall typhoons. Numerical simulations showed that the typhoon-induced net sediment flux within the spring tidal cycle could increase by 80% to 100% compared to the neap tidal cycle, indicating the significant influence of tidal conditions on sediment transport during TC events. Full article
(This article belongs to the Section Physical Oceanography)
19 pages, 4182 KiB  
Article
Advancing Sustainability and Performance with Crushed Bottom Ash as Filler in Polymer-Modified Asphalt Concrete Mixtures
by Yeong-Min Kim, Kyungnam Kim and Tri Ho Minh Le
Polymers 2024, 16(12), 1683; https://doi.org/10.3390/polym16121683 - 13 Jun 2024
Viewed by 322
Abstract
Amid the growing demand for sustainable pavement solutions and the need to incorporate recycled materials into construction practices, this study explored the viability of using crushed thermal power plant bottom ash as a filler in polymer-modified asphalt concrete mixtures. Conventional lime filler was [...] Read more.
Amid the growing demand for sustainable pavement solutions and the need to incorporate recycled materials into construction practices, this study explored the viability of using crushed thermal power plant bottom ash as a filler in polymer-modified asphalt concrete mixtures. Conventional lime filler was replaced with bottom ash at varying levels (0%, 25%, 50%, and 75%), and the resulting mixtures were evaluated using several performance tests. The optimal replacement level was determined to be 25%, based on the results of the indirect tensile strength (ITS) test. Comparisons between the control mixture and the 25% bottom ash-modified mixture were conducted using the dynamic modulus test, Cantabro test, Hamburg wheel tracking (HWT) test, and tensile strength ratio (TSR) test. The findings indicate that the 25% bottom ash-modified mixture demonstrated improved performance across multiple parameters. The HWT test showed enhanced rut durability, with a recorded depth of 7.56 mm compared to 8.9 mm for the control mixture. The Cantabro test results revealed lower weight loss percentages for the modified mixture, indicating better abrasion resistance. The dynamic modulus test indicated higher resilience and stiffness in both high- and low-frequency stages. The TSR test highlighted improved moisture resistance, with higher TSR values after 10 wet-drying cycles. These improvements are attributed to the fine particle size and beneficial chemical composition of bottom ash, which enhance the asphalt mixture’s density, binder-aggregate adhesion, and overall durability. The results suggest that incorporating 25% crushed bottom ash as a filler in polymer-modified asphalt concrete mixtures is a viable and sustainable approach to improving pavement performance and longevity. Full article
(This article belongs to the Special Issue Advances in Sustainable Polymeric Materials, 3rd Edition)
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17 pages, 10721 KiB  
Article
Bacterial Diversity and Vertical Distribution Patterns in Sandy Sediments: A Study on the Bacterial Community Structure Based on Environmental Factors in Tributaries of the Yangtze River
by Tian Zhang, Weibo Wang, Yifei Leng, Yu Huang, Wen Xiong and Fengyi Chang
Microorganisms 2024, 12(6), 1178; https://doi.org/10.3390/microorganisms12061178 - 11 Jun 2024
Viewed by 356
Abstract
Bacterial diversity and its distribution characteristics in sediments are critical to understanding and revealing biogeochemical cycles in sediments. However, little is known about the relationship between biogeochemistry processes and vertical spatial distribution of bacterial communities in sandy sediments. In this study, we used [...] Read more.
Bacterial diversity and its distribution characteristics in sediments are critical to understanding and revealing biogeochemical cycles in sediments. However, little is known about the relationship between biogeochemistry processes and vertical spatial distribution of bacterial communities in sandy sediments. In this study, we used fluorescence quantitative PCR, high-throughput sequencing technology and statistical analysis to explore the vertical distribution pattern of bacterial community diversity and its influencing factors in sandy sediments of the Yangtze River Basin. The aim is to enrich the understanding of the ecological characteristics and functions of bacteria in river ecosystems. The results showed that both sediment bacterial abundance and diversity showed a gradual decrease from surface to bottom in the vertical distribution. The main environmental factors that influenced the bacterial distribution pattern were pore water dissolved oxygen (DO), total nitrogen (TN) concentration and sediment nitrogen (N) content. The dominant bacterial species, Massilia and Flavobacterium, are suitable for growth and reproduction in high oxygen and nutrient-richer environments, while Limnobacter prefers low oxygen or anaerobic conditions. The vertical distribution pattern of bacteria and its influencing factors in river sandy sediment found in this study differ from the results in mud sediment, which may be related to the larger granular gap between sandy sediment and the lower content of organic matter. The findings of this study further our understanding of the distribution patterns and ecological preferences of microbial communities in river sediments, providing insights into how these communities may adapt to varying environmental conditions. Full article
(This article belongs to the Special Issue Microbial Communities in Aquatic Environments)
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22 pages, 26927 KiB  
Article
Experimental Study on the Process of Submerged Arc Welding for Nickel-Based WC Flux-Cored Wire on Descaling Roll
by Chang Li, Lei Feng, Xing Han, Fenghua Luo and Han Sun
Coatings 2024, 14(6), 734; https://doi.org/10.3390/coatings14060734 - 8 Jun 2024
Viewed by 644
Abstract
Descaling roll is a key component used to remove iron oxide on billet surface in hot rolling production lines, and its surface properties have a significant effect on the quality of hot rolling products. The descaling roll is in bad service condition and [...] Read more.
Descaling roll is a key component used to remove iron oxide on billet surface in hot rolling production lines, and its surface properties have a significant effect on the quality of hot rolling products. The descaling roll is in bad service condition and subjected to the dynamic impact caused by high-pressure water erosion and high temperature billet descaling process for a long time. Under the action of high temperature, strong wear, multi-cycle heat, force, flow and multi-field strong coupling, the surface is prone to wear and corrosion failure, which affects the continuous rolling production. Submerged arc welding provides an effective way to repair and strengthen the descaling roll surface. The content of WC hard phase has a significant effect on welding quality. At the same time, direct submerged arc welding of Ni based WC wire on the descaling roll surface is easy to cause cracks, and a gradient synergistic strengthening effect can be formed by setting the transition bottom layer in welding. At present, there is a lack of experiments related to the preparation of flux-cored wire with different contents and the overlaying for the bottom submerged arc welding. Relevant studies are urgently needed to further reveal the welding process mechanism to provide significant theoretical support for the preparation of wire materials and the improvement of welding quality. In this paper, 30% and 60% WC flux-cored wires were prepared by employing Ni-Cr-B-Si alloy powder as the base powder, and submerged arc welding tests were conducted on the descaling roll, preparing three welding layers, namely 70% NiCrBSi + 30% WC without the bottom layer, 70% NiCrBSi + 30% WC with the bottom layer, and 40% NiCrBSi + 60% WC with the bottom layer. The properties of the welding layer were evaluated by SEM, XRD, EDS, hardness, friction and wear, corrosion and impact experiments. The results show that the WC hard phase added in the filler metal has dissolved and formed a new phase with other elements in the melting pool. The surfacing layer mainly contains Fe-Ni, Cr-C, Fe3Si, Ni3C and other phases. The surfacing layer prepared by a different amount of WC flux-cored wire and the surfacing layer with or without the bottom layer have great differences in microstructure and properties. This study lays a significant theoretical foundation for optimizing the submerged arc welding process and preparing welding materials for the descaling roll and has significant practical significance and application value. Full article
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17 pages, 7703 KiB  
Article
Effects of Wetting–Drying Cycles on the Macro and Micro Properties of the Cement-Stabilized Soil with Curing Agent
by Wenjun Hu, Kun Li, Wenhao Yin, Han Zhang, Yi Xue, Yutong Han and Pingyun Liu
Buildings 2024, 14(6), 1716; https://doi.org/10.3390/buildings14061716 - 7 Jun 2024
Viewed by 430
Abstract
Cement-stabilized soil is a commonly used pavement base/bottom base material. Adding a suitable curing agent to cement-stabilized soil can effectively reduce the dosage of cement, meet the strength requirements, and also greatly improve its water stability. In this paper, three kinds of cement [...] Read more.
Cement-stabilized soil is a commonly used pavement base/bottom base material. Adding a suitable curing agent to cement-stabilized soil can effectively reduce the dosage of cement, meet the strength requirements, and also greatly improve its water stability. In this paper, three kinds of cement dosage (6%, 8%, and 10%) of cement-stabilized soil were selected to add a 0.04% organic liquid curing agent, and then compared with high-dose cement (10% and 12%)-stabilized soil. The influence of wetting–drying cycles on the mechanical properties of the five stabilized soils was discussed. The mineral composition of cement-stabilized soils before and after the addition of a curing agent was analyzed by X-ray diffraction (XRD), and the microscopic morphology of 10% cement-stabilized soils with a curing agent was studied by scanning electron microscopy (SEM). The macroscopic test shows that the unconfined compressive strength of solidified cement-stabilized soil can be divided into three stages with the increase in the times of the wetting–drying cycles, which are the rapid decay stage, stable enhancement stage, and stable decay stage. The wetting–drying stability coefficient first increases, and then decreases with the increase in the times of the wetting–drying cycles. The microscopic test shows that the addition of a curing agent can enhance the content of hydration products in the cement-stabilized soil specimen; at the curing age of 28 d, with the increase in the times of the wet–dry cycles, the structure of the solidified cement-stabilized soil gradually broke down. The surface porosity P and pore diameter d showed an overall upward trend but decreased at the fifth wetting–drying cycle. The pore orientation weakened. The results show that the resistance of cement-stabilized soil with a curing agent is obviously better than that of cement-stabilized soil under wet–dry conditions. Full article
(This article belongs to the Special Issue Advances in Road Pavements)
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22 pages, 7006 KiB  
Article
4E Study and Best Performance Analysis of a Hydrogen Multi-Generation Layout by Waste Energy Recovery of Combined SOFC-GT-ORC
by Mohammad Zoghi, Nasser Hosseinzadeh, Saleh Gharaie and Ali Zare
Energies 2024, 17(11), 2791; https://doi.org/10.3390/en17112791 - 6 Jun 2024
Viewed by 331
Abstract
Different approaches have been suggested for the waste heat recovery of high-temperature exhausted gas of a solid oxide fuel cell (SOFC). In such systems, mostly gas turbine (GT) and organic Rankine cycle (ORC) are added as bottoming systems to the SOFC (Configuration 1). [...] Read more.
Different approaches have been suggested for the waste heat recovery of high-temperature exhausted gas of a solid oxide fuel cell (SOFC). In such systems, mostly gas turbine (GT) and organic Rankine cycle (ORC) are added as bottoming systems to the SOFC (Configuration 1). However, the SOFC-GT-ORC has a considerable amount of waste energy which can be recovered. In the present research, the waste energy of ORC in the heat rejection stage and the residual exhausted gas of the system were recovered by a thermoelectric generator (TEG) and a hot water unit, respectively. Then, the extra produced power in the TEG was directed to a proton exchange membrane electrolyzer and a reverse osmosis desalination unit (RODU) for hydrogen and potable water outputs. The performance of SOFC-GT, Configuration 1, and Configuration 2 was compared through a 4E (energy, exergy, exergy-economic, and environmental) analysis. In the best performance point, the exergy efficiency and unit cost of product (UCOP) of SOFC-GT were obtained as 69.41% and USD 26.53/GJ. The exergy efficiency increased by 2.56% and 2.86%, and the UCOP rose by 0.45% and 12.25% in Configurations 1 and 2. So, the overall performance of Configuration 1 was acceptable and Configuration 2 led to the highest exergy efficiency, while its economic performance was not competitive because of the high investment cost of RODU. Full article
(This article belongs to the Section A: Sustainable Energy)
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21 pages, 6499 KiB  
Article
Lateral Performance Analysis of Trapezoidal Orthogonal Stiffened Steel Plate Shear Walls
by Hong Zheng, Lele Cai, Jiawei Qin, Yuxi Mi, Liqiang Jiang, Xiaoming Ma and Zhiwei Sun
Buildings 2024, 14(6), 1634; https://doi.org/10.3390/buildings14061634 - 3 Jun 2024
Viewed by 231
Abstract
This paper introduces a trapezoidal orthogonal stiffener steel plate shear wall (TSW). The finite element model of the TSW was developed following the validation of low-cycle repeated tests conducted on a single-span double-layer steel plate shear wall. The paper studies the effects of [...] Read more.
This paper introduces a trapezoidal orthogonal stiffener steel plate shear wall (TSW). The finite element model of the TSW was developed following the validation of low-cycle repeated tests conducted on a single-span double-layer steel plate shear wall. The paper studies the effects of the flat steel plate thickness, stiffener thickness, stiffener height, and stiffener bottom width on the seismic performance of TSW. Building upon these findings, a theoretical formula for the ultimate shear capacity of TSW was developed. The results prove the following: (1) By changing the flat steel plate thickness, the stiffener thickness, and the stiffener height, the seismic behavior of TSW can be enhanced. It is suggested that the flat steel plate thickness is 4~6 mm, the stiffener thickness is 4~6 mm, and the stiffener height is not more than 60 mm, while the effect of the stiffener bottom width on the seismic behavior of TSW can be neglected. (2) The maximum error is 22.16%, compared to the theoretical value of TSW ultimate shear capacity with the finite element simulation value. However, as the finite element results surpass the test results, it indicates that the formula-derived results are unsafe, necessitating a recommendation for correction. Full article
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24 pages, 11260 KiB  
Article
Fragility Analysis of Step-Terrace Frame-Energy Dissipating Rocking Wall Structure in Mountain Cities
by Youfa Yang, Yingwei Jia and Hongshen Jin
Appl. Sci. 2024, 14(11), 4731; https://doi.org/10.3390/app14114731 - 30 May 2024
Viewed by 234
Abstract
Rocking walls can control the overall deformation pattern and the distribution of plastic energy dissipation in structures, suppressing the occurrence of weak layers. In the case of step-terrace frame structures, issues such as severe lateral stiffness irregularities, abrupt changes in floor-bearing capacity, and [...] Read more.
Rocking walls can control the overall deformation pattern and the distribution of plastic energy dissipation in structures, suppressing the occurrence of weak layers. In the case of step-terrace frame structures, issues such as severe lateral stiffness irregularities, abrupt changes in floor-bearing capacity, and concentrated deformation in upper ground layers exist. To improve the yielding and failure modes of step-terrace frame structures in mountainous regions, this paper proposes a structural system combining step-terrace frame structures with energy dissipation rocking walls attached to their bottoms, aiming to control the yielding mechanism of the structure, further reduce the seismic response, limit residual deformation, and propose a structural system of step-terrace frame structures with buckling-restrained braces (BRBs) and energy dissipation rocking walls. Two sets of numerical models for step-terrace frame structures with different numbers of dropped layers and spans were established. Through simulating low-cycle repeated loading tests on step-terrace frame structures, the rationality of the models and parameters was verified. Incremental dynamic analysis (IDA) was employed to systematically investigate the vulnerability of step-terrace frame structures with energy dissipation rocking walls under different dropped layer and span configurations. This investigation covered aspects such as IDA curve clusters, percentile curves, seismic demand models, fragility functions, failure state probabilities, vulnerability indices, collapse resistance factors, and safety margins. The results indicated that the change in dropped layer numbers had a far greater impact on the vulnerability of step-terrace frame structures with energy dissipation rocking walls than the change in dropped span numbers. Under seismic excitations with the same peak ground acceleration (PGA), rocking walls can limit the depth of structural plasticity development, reduce the dispersion of peak responses, and lower the probability of exceeding various performance levels, thereby exhibiting good collapse resistance. The addition of buckling-restrained braces (BRBs) can further enhance the seismic performance and collapse resistance of the rocking wall frame structure. By analyzing the correlation between seismic intensity measures and peak structural responses, the validity of using peak ground acceleration as a scaling indicator for incremental dynamic analysis (IDA) has been verified. Full article
(This article belongs to the Section Civil Engineering)
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35 pages, 4293 KiB  
Article
Investigation of a Biomass-Driven Cogeneration System Integrated with an Externally Fired Gas Turbine, Organic Rankine Cycle, and Absorption Refrigeration Cycle: Thermodynamic and Exergoeconomic Analyses and Optimization
by Jie Ren, Zuoqin Qian, Xinyu Wang, Weilong Huang and Baolin Wang
Sustainability 2024, 16(11), 4495; https://doi.org/10.3390/su16114495 - 25 May 2024
Viewed by 533
Abstract
The utilization of biomass for multi-generation systems is garnering significant interest due to its potential in conserving primary energy and mitigating greenhouse gas emissions. However, enhancing its energy efficiency remains a critical challenge. This study introduces an innovative cogeneration system that combines biomass [...] Read more.
The utilization of biomass for multi-generation systems is garnering significant interest due to its potential in conserving primary energy and mitigating greenhouse gas emissions. However, enhancing its energy efficiency remains a critical challenge. This study introduces an innovative cogeneration system that combines biomass gasification with an externally fired gas turbine, organic Rankine cycle, and absorption refrigeration cycle. It undergoes thorough thermodynamic and exergoeconomic evaluations, with a dual-objective optimization conducted to identify the optimal operational conditions that achieve the highest exergy efficiency while minimizing product cost. The findings reveal that, in the base case, the thermal efficiency, exergy efficiency, and sum unit cost of the product (SUCP) of the system are 66.36%, 32.04%, and 8.71 USD/GJ, respectively. A parametric study illustrates that elevating the air compressor pressure ratio or the temperature difference at the cold end enhances thermal efficiency but reduces exergy efficiency. Additionally, the lowest unit cost of the product is attainable by optimizing the gas turbine inlet temperature. The performance of the system shows negligible sensitivity to the turbine inlet pressure of a bottoming organic Rankine cycle. Finally, optimization demonstrates a 9.7% increase in exergy efficiency and a 1.8% rise in the SUCP compared to the baseline scenario. The study suggests integrating with other energy sources for diversified product outputs and conducting environmental analyses in future research. Full article
(This article belongs to the Special Issue Biomass Energy Usage for Sustainable Development)
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17 pages, 4189 KiB  
Article
Multispectral Inversion of Citrus Multi-Slope Evapotranspiration by UAV Based on Modified RSEB Model
by Shijiang Zhu, Zhiwei Zhang, Chenfei Duan, Zhen Lin, Kun Hao, Hu Li and Yun Zhong
Water 2024, 16(11), 1520; https://doi.org/10.3390/w16111520 - 25 May 2024
Viewed by 469
Abstract
Evaptotranspiration (ETc) is a crucial link in the farmland water cycle process. To accurately obtain the citrus ETc in different slope positions, the METRIC, RSEB, and FAO Penman–Monteith (P-M) models were constructed based on unmanned aerial vehicle (UAV) multispectral images [...] Read more.
Evaptotranspiration (ETc) is a crucial link in the farmland water cycle process. To accurately obtain the citrus ETc in different slope positions, the METRIC, RSEB, and FAO Penman–Monteith (P-M) models were constructed based on unmanned aerial vehicle (UAV) multispectral images to invert the ETc values. The ETc of citrus calculated by the P-M model was used as a reference standard, and the accuracy of the ETc inversion was evaluated by the METRIC model and the RSEB model. The results showed that the R2, RMSE, and SE of the METRIC model and the RSEB model were 0.396 and 0.486, 4.940 and 3.010, and 4.570 and 2.090, respectively, indicating a higher accuracy of the RSEB model for inverting the ETc values. Furthermore, the accuracy of the RSEB model could be improved by introducing the optimal correction coefficient (after correction: RMSE = 1.470, SE = 0.003). Based on the modified RSEB model, the ETc values of the citrus in different slope positions were obtained. We also found that the middle slope ETc > the top slope ETc > the bottom slope ETc, indicating that the slope position indeed affected the citrus ETc. This research provides a favorable framework for the ETc inversion, and the results are of theoretical and practical importance to realize crop water conservation. Full article
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21 pages, 10163 KiB  
Article
Photovoltaic Manufacturing Factories and Industrial Site Environmental Impact Assessment
by Peter Brailovsky, Lorena Sanchez, Dilara Subasi, Jochen Rentsch, Ralf Preu and Sebastian Nold
Energies 2024, 17(11), 2540; https://doi.org/10.3390/en17112540 - 24 May 2024
Viewed by 529
Abstract
Life cycle inventories (LCIs) and life cycle assessments (LCAs) of photovoltaic (PV) modules and their components focus on the operations of PV factories, but the factories and industrial site product and construction stages are either not or only partially tackled. This work contributes [...] Read more.
Life cycle inventories (LCIs) and life cycle assessments (LCAs) of photovoltaic (PV) modules and their components focus on the operations of PV factories, but the factories and industrial site product and construction stages are either not or only partially tackled. This work contributes through the bottom-up, model-based generation of LCIs and LCAs for setting up a vertically integrated 5 GWp/a PV industrial site, including the manufacturing of silicon ingots, wafers, solar cells, and PV modules, on a 50 ha greenfield location. Two comparative LCAs are performed. The first compares the annualized environmental impacts of the developed LCI sets with four existing inventories in the Ecoinvent v3.8 database. The second comparative LCA explores the environmental impact differences concerning the industrial site when using different building systems for the factories. Here, the reference system with a steel structure is compared with two alternative building systems: precast concrete and structural timber. The results show that the wafer, cell, and module factories’ annualized environmental impacts with the Ecoinvent LCIs are strongly overestimated. For the ingot factory, the opposite result is identified. The impacts of all four factories show reductions of between 11.7% and 94.3% for 14 of the 15 impact categories. High mean environmental impact shares of 79.0%, 78.2% and 79.2% for the steel, precast concrete and timber structural building systems, respectively, are generated at the product stage. The process and facilities equipment generates 54.2%, 54.4% and 58.2% of the total product and construction stages’ mean environmental impact shares. The proposed alternative timber building system reduces the environmental impacts in 14 of the 15 evaluated categories, with reductions ranging from 1.1% to 12.4%. Full article
(This article belongs to the Special Issue Life Cycle Assessment in Renewable and Sustainable Energy)
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21 pages, 4451 KiB  
Article
Fungal and Prokaryotic Communities in Soil Samples of the Aral Sea Dry Bottom in Uzbekistan
by Alexandra Šimonovičová, Eva Pauditšová, Sanja Nosalj, Medetbay Oteuliev, Nikola Klištincová, Francesca Maisto, Lucia Kraková, Jelena Pavlović, Katarína Šoltys and Domenico Pangallo
Soil Syst. 2024, 8(2), 58; https://doi.org/10.3390/soilsystems8020058 - 21 May 2024
Viewed by 490
Abstract
Due to the falling water level in the Aral Sea and Muynak Lake, the content of salts dissolved in the water has gradually increased, and toxic elements have been deposited at the lake’s bottom and subsequently washed into the Aral region by the [...] Read more.
Due to the falling water level in the Aral Sea and Muynak Lake, the content of salts dissolved in the water has gradually increased, and toxic elements have been deposited at the lake’s bottom and subsequently washed into the Aral region by the river. Bacteria, archaea and fungi are crucial for the cycling of several important inorganic nutrients in soils. From 15 genera and 31 species of recovered microscopic filamentous fungi, a big group was melanized, of which most of them were also phytopathogenic. The second group consisted of keratinophilic species. Isolated bacteria mainly included members of the genera Arthrobacter, Bacillus, Massilia, Rhodococcus and Nocardiopsis. High-throughput sequencing analysis permitted a better view of the mycobiome and prokaryotic communities (comprising archaea). The cultivation and sequencing approaches were shown to be complementary. The aim of the work was to identify soil microorganisms, including the order Halobacteriales, and to discover the differences in species diversity depending on soil salinity and the presence of PTEs in soil. Full article
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20 pages, 1996 KiB  
Article
Cellular Responses Induced by NCT-503 Treatment on Triple-Negative Breast Cancer Cell Lines: A Proteomics Approach
by Ioana-Ecaterina Pralea, Radu-Cristian Moldovan, Adrian-Bogdan Țigu, Cristian-Silviu Moldovan, Eva Fischer-Fodor and Cristina-Adela Iuga
Biomedicines 2024, 12(5), 1087; https://doi.org/10.3390/biomedicines12051087 - 14 May 2024
Cited by 1 | Viewed by 724
Abstract
Breast cancer (BC) remains one of the leading causes of mortality among women, with triple-negative breast cancer (TNBC) standing out for its aggressive nature and limited treatment options. Metabolic reprogramming, one of cancer’s hallmarks, underscores the importance of targeting metabolic vulnerabilities for therapeutic [...] Read more.
Breast cancer (BC) remains one of the leading causes of mortality among women, with triple-negative breast cancer (TNBC) standing out for its aggressive nature and limited treatment options. Metabolic reprogramming, one of cancer’s hallmarks, underscores the importance of targeting metabolic vulnerabilities for therapeutic intervention. This study aimed to investigate the impact of de novo serine biosynthetic pathway (SSP) inhibition, specifically targeting phosphoglycerate dehydrogenase (PHGDH) with NCT-503, on three TNBC cell lines: MDA-MB-231, MDA-MB-468 and Hs 578T. First, MS-based proteomics was used to confirm the distinct expression of PHGDH and other SSP enzymes using the intracellular proteome profiles of untreated cells. Furthermore, to characterize the response of the TNBC cell lines to the inhibitor, both in vitro assays and label-free, bottom-up proteomics were employed. NCT-503 exhibited significant cytotoxic effects on all three cell lines, with MDA-MB-468 being the most susceptible (IC50 20.2 ± 2.8 µM), while MDA-MB-231 and Hs 578T showed higher, comparable IC50s. Notably, differentially expressed proteins (DEPs) induced by NCT-503 treatment were mostly cell line-specific, both in terms of the intracellular and secreted proteins. Through overrepresentation and Reactome GSEA analysis, modifications of the intracellular proteins associated with cell cycle pathways were observed in the MDA-MBs following treatment. Distinctive dysregulation of signaling pathways were seen in all TNBC cell lines, while modifications of proteins associated with the extracellular matrix organization characterizing both MDA-MB-231 and Hs 578T cell lines were highlighted through the treatment-induced modifications of the secreted proteins. Lastly, an analysis was conducted on the DEPs that exhibited greater abundance in the NCT-503 treatment groups to evaluate the potential chemo-sensitizing properties of NCT-503 and the druggability of these promising targets. Full article
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15 pages, 4521 KiB  
Article
Electro-Coloring Mechanism of Aluminum Anodic Oxides in Tin-Based Electrolytes
by Pinar Afsin, Can Akyil, Kürşat Kazmanlı and Mustafa Ürgen
Coatings 2024, 14(5), 616; https://doi.org/10.3390/coatings14050616 - 13 May 2024
Viewed by 668
Abstract
A method for accurately determining the chemical composition of deposits at the bottom of pores during the electrocoloring (e-coloring) of aluminum anodic oxide (AAO) layers in tin-based solutions is developed. The aluminum samples were AC e-colored after DC sulfuric anodization. Free-standing, tin e-colored [...] Read more.
A method for accurately determining the chemical composition of deposits at the bottom of pores during the electrocoloring (e-coloring) of aluminum anodic oxide (AAO) layers in tin-based solutions is developed. The aluminum samples were AC e-colored after DC sulfuric anodization. Free-standing, tin e-colored aluminum oxide film was obtained by selective dissolution of the metallic aluminum from the AAO in copper chloride solution to access the deposit directly at the bottom of the pore. This allowed us to conduct XPS analysis directly on the deposits at pore bottoms without any interference from the base material or insulating barrier layer. The results revealed the presence of a mixture of tin oxide and metal in the deposits, which were richer in oxide content. Furthermore, a cyclic voltammetry experiment mimicking real polarization conditions during AC conditions was optimized and used to gain a deeper understanding of the electrochemical reactions that occur during AC electrocoloring. The comparison of CV results in tin-free and tin-containing electrolytes indicated that the tin deposited during a cathodic cycle is oxidized in the anodic cycle. The formation of tin-based deposits radically changed the CV behavior. The XPS and cyclic voltammetry results consistently show that the deposits formed during e-coloring comprised a mixture of metallic and oxidic tin species richer in oxide content. Full article
(This article belongs to the Section Surface Characterization, Deposition and Modification)
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16 pages, 3863 KiB  
Article
ccdC Regulates Biofilm Dispersal in Bacillus velezensis FZB42
by Lin Shao, Zizhu Shen, Meiju Li, Chenyun Guan, Ben Fan, Yunrong Chai and Yinjuan Zhao
Int. J. Mol. Sci. 2024, 25(10), 5201; https://doi.org/10.3390/ijms25105201 - 10 May 2024
Viewed by 537
Abstract
Bacillus velezensis FZB42 is a plant growth-promoting rhizobacterium (PGPR) and a model microorganism for biofilm studies. Biofilms are required for the colonization and promotion of plant growth in the rhizosphere. However, little is known about how the final stage of the biofilm life [...] Read more.
Bacillus velezensis FZB42 is a plant growth-promoting rhizobacterium (PGPR) and a model microorganism for biofilm studies. Biofilms are required for the colonization and promotion of plant growth in the rhizosphere. However, little is known about how the final stage of the biofilm life cycle is regulated, when cells regain their motility and escape the mature biofilm to spread and colonize new niches. In this study, the non-annotated gene ccdC was found to be involved in the process of biofilm dispersion. We found that the ccdC-deficient strain maintained a wrinkled state at the late stage of biofilm formation in the liquid—gas interface culture, and the bottom solution showed a clear state, indicating that no bacterial cells actively escaped, which was further evidenced by the formation of a cellular ring (biofilm pellicle) located on top of the preformed biofilm. It can be concluded that dispersal, a biofilm property that relies on motility proficiency, is also positively affected by the unannotated gene ccdC. Furthermore, we found that the level of cyclic diguanylate (c-di-GMP) in the ccdC-deficient strain was significantly greater than that in the wild-type strain, suggesting that B. velezensis exhibits a similar mechanism by regulating the level of c-di-GMP, the master regulator of biofilm formation, dispersal, and cell motility, which controls the fitness of biofilms in Pseudomonas aeruginosain. In this study, we investigated the mechanism regulating biofilm dispersion in PGPR. Full article
(This article belongs to the Section Molecular Biology)
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