Search Results (358)

Microgreens are gaining prominence as nutrient-dense foods with health-promoting activities while aligning with smart agriculture and functional food trends. They are rich in numerous bioactive compounds like phenolics, ascorbic acid, and carotenoids, which act as antioxidants, while also causing multiple other biological activities. Using advanced statistical methods, this study investigated Brassicaceae microgreens, identifying kale and Sango radish as standout varieties. Both contained 16 amino acids, with potassium and calcium as dominant minerals. Sugar and protein contents ranged from 4.29 to 4.66% and 40.27 to 43.90%, respectively. Kale exhibited higher carotenoid levels, particularly lutein (996.36 mg/100 g) and beta-carotene (574.15 mg/100 g). In comparison, Sango radish excelled in glucose metabolism (α-glucosidase inhibition: 58%) and antioxidant activities (DPPH^•: 7.92 mmol TE/100 g, ABTS^•+: 43.47 mmol TE/100 g). Both showed antimicrobial activity against Escherichia coli and Staphylococcus aureus. Kale demonstrated stronger anti-inflammatory effects, while Sango radish showed antiproliferative potential. These results, supported by PCA and correlation analysis, underscore the dual role of these microgreens as nutritious and therapeutic food additives, addressing oxidative stress, inflammation, and microbial threats. Full article

To produce plug seedlings with uniform growth and which are suitable for high-speed transplanting operations, it is essential to sow seeds precisely at the center of each plug-tray hole. For accurately determining the position of the seed covered by the substrate within individual plug-tray holes, a novel method for detecting the growth points of plug seedlings has been proposed. It employs an adaptive grayscale processing algorithm based on the differential evolution extra-green algorithm to extract the contour features of seedlings during the early stages of cotyledon emergence. The pixel overlay curve peak points within the binary image of the plug-tray’s background are utilized to delineate the boundaries of the plug-tray holes. Each plug-tray hole containing a single seedling is identified by analyzing the area and perimeter of the seedling’s contour connectivity domains. The midpoint of the shortest line between these domains is designated as the growth point of the individual seedling. For laboratory-grown plug seedlings of tomato, pepper, and Chinese kale, the highest detection accuracy was achieved on the third-, fourth-, and second-days’ post-cotyledon emergence, respectively. The identification rate of missing seedlings and single seedlings exceeded 97.57% and 99.25%, respectively, with a growth-point detection error of less than 0.98 mm. For tomato and broccoli plug seedlings cultivated in a nursery greenhouse three days after cotyledon emergence, the detection accuracy for missing seedlings and single seedlings was greater than 95.78%, with a growth-point detection error of less than 2.06 mm. These results validated the high detection accuracy and broad applicability of the proposed method for various seedling types at the appropriate growth stages. Full article

Due to cyanobacterial toxin (cyanotoxin) contamination issues in 2018, the city of Salem, Oregon, issued a 33-day do-not-drink advisory for vulnerable people among the 200,000 residents. After the incident, the state of Oregon put in place drinking water rules to require the routine testing of raw water, as well as finished water, in cases where the raw water cyanotoxin concentrations exceeded trigger values. The United States Environmental Protection Agency (EPA) total microcystins drinking water health advisory level (HAL) for small children is 0.3 µg/L. This is equivalent to the minimum reporting level (MRL) for EPA Method 546. Consequently, there was no ability to provide early warnings via toxin testing for total microcystins using the EPA method. In this study, we performed a comparison of the precision and accuracy of the enzyme-linked immunosorbent assay (ELISA) described in the EPA method to a more sensitive assay, the Streptavidin-enhanced Sensitivity (SAES) assay. Based on these precision and accuracy studies and quantitation limit determinations and confirmations, the EPA Office of Ground Water and Drinking Water (OGWDW) has concluded the SAES kit meets the requirements of EPA Method 546. With an MRL that is one-third of the original concentration, the new kit provides a small but critical window for identifying early warnings. Challenges remain with providing early warnings due to the variability in bloom dynamics; however, the new MRL allowed Oregon to lower the trigger level for susceptible systems, thereby providing an additional early warning. Full article

Iodine is a key micronutrient essential for the synthesis of thyroid hormone, which regulates metabolic processes and maintains overall health. Despite its importance, iodine deficiency is a global health issue, leading to disorders such as goiter, hypothyroidism, and developmental abnormalities. Biofortification of crops with iodine is a promising strategy to enhance the dietary iodine intake, providing an alternative to iodized salt. Curly kale (Brassica oleracea var. sabellica) is a nutrient-rich vegetable high in vitamins A, C, K; minerals; fiber; and bioactive compounds with antioxidant, anti-inflammatory, and detoxifying properties. This study evaluates the effects of diets containing iodine-biofortified curly kale (‘Oldenbor F₁’ and ‘Redbor F₁’) on iodine content, tissue iodine levels, and various biochemical parameters in laboratory rats. The biofortified curly kale was enriched with 5,7-diiodo-8-quinolinol. The iodine content in the AIN-93G (control) diet and the non-biofortified curly kale diets did not differ significantly. However, diets with 5,7-diiodo-8-quinolinol biofortified kale showed significantly higher iodine levels compared with the control diets. Tissue analysis revealed the highest iodine concentrations in the liver and kidneys of rats fed diets with biofortified curly kale, indicating better iodine bioavailability. Biochemical analysis showed that rats fed the biofortified kale diet had lower total cholesterol (TC) and triglyceride (TG) levels compared with rats fed the control diet. Additionally, the biofortified diet improved the liver function markers (ALAT, ASAT) and reduced oxidative stress markers (TBARS). The study also investigated the expression of thyroid-related genes (Slc5A5, Tpo, Dio1, Dio2) in response to diets containing biofortified kale. The results demonstrated significant changes in gene expression, indicating adaptive mechanisms to dietary iodine levels and the presence of bioactive compounds in the biofortified kale. The study also observed variations in uric acid levels, with lower concentrations in rats fed a diet with biofortified curly kale. Biofortified curly kale supports thyroid function and improves liver and kidney health by reducing oxidative stress and modulating key biochemical and genetic markers. These findings suggest that biofortified curly kale can effectively increase dietary iodine intake as a nutritional intervention to address iodine deficiency and promote overall health. Full article

There is a growing need for sustainable, efficient methods to promote plant growth and protect crops, with plant extracts offering natural, multi-component solutions. Based on previous observations, Psidium guajava, Aloe vera, Allium sativum and Medicago sativa were selected from 17 water extracts to investigate how the application times of soil sprays affect the antioxidant enzymes and secondary metabolites in fruity and leafy vegetables at different growth stages. From 1 week after sowing (WAS) to 4 WAS, all applications increased the shoot fresh weight by 42–69% in cucumbers, 40–64% in tomatoes, 46–65% in kale and 42–63% in lettuce. These applications also increased the photosynthesis, flavonoids and antioxidative enzymes (ascorbate peroxide (APOD) and guaiacol peroxidase (GPOD)), which provided the plants with a balanced supply of nutrients essential for growth. In the real world, these results show that the use of natural extracts (P. guajava and A. sativum) can be a sustainable, eco-friendly alternative to synthetic fertilizers and pesticides, helping to improve crop yields and metabolism without harming the environment. This approach could reduce the reliance on chemical inputs and promote more sustainable agricultural practices, especially in controlled environments, like greenhouses, where crops like cucumbers and kale are grown. Full article

Plant factories with artificial lighting (PFALs) are a notable choice for urban agriculture due to the system’s benefits, where light can be manipulated to enhance the product’s yield and quality. Our objective was to test the effect of light spectra with different red-blue combinations and white light on the growth, physiology, and overall quality of three baby-leaf vegetables (green lettuce, kale, and pak choi) grown in a restaurant’s PFAL. Leaf mass per area was lower under the most blue-containing treatments in all species. The performance indices (PI_abs and PI_tot) of the photosynthetic apparatus were lower under more red light with the exception of PI_abs in pak choi. Total soluble solids accumulation was diminished under most of the blue-containing LEDs, while total phenolics and antioxidant activity were induced by red-blue environments rich in blue light. Moreover, chlorophyll and carotenoid accumulation was also enhanced under blue-rich light treatments. Nitrate content was the lowest under monochromatic blue in all species. Finally, the employees were asked about their views on the PFAL within the restaurant’s compounds and they expressed positive opinions. Overall, a light environment including red and blue wavelengths proved beneficial for baby leafy vegetable production in terms of yield and quality. Full article

Kale (Brassica oleracea) is recognized as a ‘superfood’ among leafy vegetables due to its high carotenoid content and potential health benefits. This study aims to optimize ultrasound-assisted extraction (UAE) to enhance the recovery of carotenoids and other phytochemicals from upcycled kale using response surface methodology. The optimized extraction parameters for carotenoids, i.e., aqueous ethanol as solvent, temperature, and extraction time at a fixed solid-to-solvent ratio, were established using the central composite design. The optimized extraction method was compared with other reported extraction methods for total phenolic content (TPC) and total antioxidant capacity (ferric reducing antioxidant power (FRAP) and 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging). Ultra-high-performance liquid chromatography–electrospray ionization–mass spectrometry (UPLC-ESI-MS) analysis was also performed. Under the optimized UAE conditions of 100% ethanol at 57 °C for 30 min, total carotenoid content of 392 µg/g dry weight (DW) was observed, though the predicted carotenoid content was 550 µg/g DW. Under these conditions, TPC, FRAP, and DPPH were 10.5 mg gallic acid equivalents/g DW, 13.9 µmole Trolox equivalence/g DW, and DPPH radical scavenging activity as IC₅₀ of 2.04 ± 0.31 mg/mL, respectively. The UPLC-ESI-MS analysis showed the highest total phytochemicals recovered through microwave-assisted extraction, followed by UAE, compared to other tested extraction methods. In conclusion, the established optimized UAE process significantly enhanced the yield and quality of recovered phytochemicals from upcycled kale. Full article

Brassica oleracea is a complex species incorporating a great variety of vegetable types, including cabbage, cauliflower, broccoli, kale, and others. Southern Italy, and especially the Puglia region, is rich in B. oleracea landraces. In this study, genotyping-by-sequencing (GBS) was applied to a germplasm panel of 82 samples, mostly landraces and some commercial varieties, belonging to various morphotypes of B. oleracea. Population structure (K = 2), principal component (PCA), and phylogenetic analyses resulted in a general subdivision of our samples into two large lineages: the types used for their leaves (LHL) and those consumed for their flower heads (AIL). Going deeper inside, the different morphotypes were mostly grouped into specific clusters, and a clear separation of particular landraces, such as the Mugnoli and Cima nera broccoli, was observed in the structure analysis (K = 7), as well as in the PCA and in the Neighbor-Joining tree. The calculation of the pairwise fixation index (F_ST, threshold > 0.50) between LHL and AIL types (based on population structure analysis at K = 2) provided 456 outlier single nucleotide polymorphisms (SNPs). Among the corresponding orthologs annotated in Arabidopsis, we identified several genes involved in flower/inflorescence development, cellular proliferation, etc. Overall, our investigation provides useful information on the knowledge of early domesticated landraces of B. oleracea and allows for the attribution of unknown material to the appropriate taxonomical ranking. The analysis of outlier SNPs has highlighted signatures of molecular divergence between LHL and AIL lineages. Full article

Rape (Brassica napus) is an important oilseed crop widely cultivated worldwide. Due to its relatively short evolutionary and domestication history, its intra-species genetic diversity is limited. Radish (Raphanus sativus), belonging to a different genus but the same family as B. nupus, possesses an abundance of excellent gene resources. It is commonly used for B. nupus germplasm improvement and genetic basis expansion, making it one of the most important close relatives for distant hybridization. In the present study, a novel method for detecting alien chromosome fragments, called Anchor Mapping of Alien Chromosome (AMAC) was used to identify radish chromosome segments in the progeny of rape–radish interspecific hybrids. Based on the AMAC method, 126,861 pairs of IP (Intron Polymorphism) and 76,764 pairs of SSR (Simple Sequence Repeat) primers were developed using the radish Rs1.0 reference genome. A total of 44,176 markers (23,816 pairs of IP and 20,360 pairs of SSR markers) were predicted to be radish genome specific-single-locus (SSL) markers through electronic PCR analysis among four R. sativus, one B. napus, one B. rapa, one B. juncea, and one B. juncea reference genome. Among them, 626 randomly synthesized SSL markers (478 SSL IP markers and 148 SSL SSR markers) were used to amplify the genome of 24 radish samples (R. sativus), 18 rape (B. napus), 2 Chinese cabbage (B. rapa), 2 kale (B. oleracea), and 2 mustard (B. juncea) samples, respectively. Then, 333 SSL markers of the radish genome were identified, which only amplified in the radish genome and not in any Brassica species genome, including 192 IP markers and 141 SSR markers. Furthermore, these validated SSL markers were used to identify alien chromosome fragments in Ogura-CMS restorer line 16C, Ogura-CMS sterile line 81A, and their hybrid-Yunyouza15. In 16C, one marker, Rs1.0025823_intron_3, had an amplification product designated as anchor marker for the alien chromosome fragment of 16C. Afterwards, four novel radish genome-specific IP markers were found to be flanking the anchor marker, and it was determined that the alien chromosome segment in 16C originated from the region 8.4807–11.7798 Mb on radish chromosome R9, and it was approximately 3.2991 Mb in size. These results demonstrate that the AMAC method developed in this study is efficient, convenient, and cost-effective for identifying excellent alien chromosome fragments/genes in distant hybrid progeny, and it can be applied to the molecular marker-assisted breeding and hybrid identification of radish and Brassica crop species. Full article

The potential of blue light (BL) and sous-vide (S-V) as a novel approach for food preservation was investigated via measurements of the total phenolic content (TPC), antioxidative activity, color, and their antibacterial effect on Listeria monocytogenes in two versions of laboratory-prepared kale pesto, with and without the addition of turmeric. The TPC ranged from 85 to 208 mg/100 g GAE d.m. and 57 to 171 mg/100 g GAE d.m., respectively. In both versions, the highest TPC was in the blue light–sous-vide samples, while the lowest was after the sous-vide, with a loss of polyphenols of almost 40% during storage when turmeric was absent. Antioxidative capabilities of the pesto were initially estimated at 54.07 and 7.46 µmol TE/g d.m., respectively, indicating significant bioactivity enhancement by turmeric. In turmeric-enriched pesto, sous-vide decreased the antioxidative activity levels by 12% in fresh pesto and by 45% during storage. Meanwhile, blue light compensated for the losses caused by the sous-vide treatment. Although the hue angle (h°) of sous-vide pesto was lower than that of blue light pesto in most samples, sequential BL and S-V ultimately yielded the lowest h°. The sequential BL and S-V treatment resulted in a 1.7 log reduction in the L. monocytogenes population, whereas adding turmeric increased the treatment efficacy by another 2.0 logs. Thus, as a source of photosensitizing molecules, turmeric was highly antibacterial after photothermal activation with blue light and sous-vide. This study suggests that blue light could be an effective (pre)treatment used on pesto sauces to preserve bioactivity and to improve safety when enriched with a natural additive like turmeric. Full article

This study investigates Xanthomonas campestris pv. campestris (Xcc) incidence rates in cabbage, kale, and kohlrabi, focusing on the roots and stems over a 15-day period after sowing. Seeds were inoculated with Xcc, and infection levels were monitored using confocal microscopy combined with fluorescence in situ hybridization. Significant differences in incidence rates were observed across the 15 days using a scale from 0 to 5 (0–100% incidence). Kale exhibited the highest mean incidence rates in roots (3.64), while cabbage and kohlrabi showed greater variability (3.4–3.44). Stem infections were more severe, with kale showing the highest mean levels (4.16), followed by kohlrabi (4.0), and cabbage (3.68). In cabbage roots, incidence rates increased until day 11, after which a significant decline was noted, whereas stem Xcc incidence rates remained stable. Kale roots peaked on day 9, with significant fluctuations on days 13 and 15. Kohlrabi displayed moderate, stable root incidence rates over the 15-day period. In stems, kohlrabi showed a significant increase on day 11, followed by a drop on day 15. Regression analysis revealed a significant positive linear correlation between incidence rate and days of observation in kohlrabi roots. However, no significant trends were observed in stem infections across all species, where incidence rates were high and stable from day 7 onwards. Newly acquired data indicate that the incidence rate of Xcc development depends on the type of vegetable. However, the bacterial occurrence in the root parts does not always correspond with the intensity of Xcc colonization in the stem section of the plants. Even at lower levels of Xcc occurrence in the roots, damage to the vascular bundles can be fatal. These findings offer valuable insights into Xcc infection dynamics, contributing to improved disease management strategies for Brassica crops. Full article

FCS-like zinc finger (FLZ) proteins are plant-specific regulatory proteins, which contain a highly conserved FLZ domain, and they play critical roles in plant growth and stress responses. Although the FLZ family has been systematically characterized in certain plants, it remains underexplored in Brassica species, which are vital sources of vegetables, edible oils, and condiments for human consumption and are highly sensitive to various abiotic stresses. Following the whole-genome triplication events (WGT) in Brassica, elucidating how the FLZ genes have expanded, differentiated, and responded to abiotic stresses is valuable for uncovering the genetic basis and functionality of these genes. In this study, we identified a total of 113 FLZ genes from three diploid Brassica species and classified them into four groups on the basis of their amino acid sequences. Additionally, we identified 109 collinear gene pairs across these Brassica species, which are dispersed among different chromosomes, suggesting that whole-genome duplication (WGD) has significantly contributed to the expansion of the FLZ family. Subcellular localization revealed that six representative BolFLZ proteins are located in the nucleus and cytoplasm. Yeast two-hybrid assays revealed that 13 selected BolFLZs interact with BolSnRK1α1 and BolSnRK1α2, confirming the conservation of the SnRK1α-FLZ module in Brassica species. Expression profile analysis revealed differential expression patterns of BolFLZ across various tissues. Notably, the expression levels of seven BolFLZ genes out of the fifteen genes analyzed changed significantly following treatment with various abiotic stressors, indicating that the BolFLZ genes play distinct physiological roles and respond uniquely to abiotic stresses in Brassica species. Together, our results provide a comprehensive overview of the FLZ gene family in Brassica species and insights into their potential applications for enhancing stress tolerance and growth in Chinese kale. Full article

With rising populations and increasing food consumption, the demand for food is placing significant strain on freshwater resources. Exploring crops that can thrive under saline conditions is crucial to ensuring food security. Although brackish and seawater is abundant, it is generally unsuitable for irrigation. However, some plants exhibit tolerance to moderate levels of salinity. This study investigated the effects of varying light intensities (150 and 250 photosynthetic photon flux densities) and salinity levels (<1.5, 5, 10, and 17 parts per thousand, equivalent to <26, 86, 171, and 291 millimolars) on the growth and nutrient composition of Russian kale (Brassica oleracea) grown in indoor hydroponics. The experiment was conducted over five months, from September 2023 to January 2024. The results revealed that a light intensity of 250 PPFD and salinity levels of <1.5–5 ppt (<26–86 mM) were optimal for maximizing the biomass yield of the kale, whereas a significant reduction in the yield was observed at salinity levels exceeding 10 ppt (171 mM). In contrast, the dry matter percentage was significantly higher at 17 ppt (291 mM). The macronutrient contents, particularly the total Kjeldahl nitrogen (TKN), total phosphorus (TP), and magnesium (Mg), were consistent across both light intensities (150–250 PPFDs) and at salinity levels between <1.5 and 10 ppt (<26–171 mM) but were reduced at 17 ppt (291 mM). The micronutrient concentrations, such as those of copper (Cu), iron (Fe), and zinc (Zn), were higher at the lower light intensity (150 PPFD) across the salinity levels. These findings suggest that optimizing the light conditions is essential for enhancing the nutritional value of kale in saline environments. These outcomes are particularly vital for improving agricultural productivity and resilience in salt-affected regions, thereby supporting broader food security and sustainability goals. Full article

In recent decades, the aquaculture industry has seen exponential growth worldwide, surpassing other food production sectors. This review aims to explore the dynamics of aqua feed production, particularly the shift from conventional to local feed production in Africa, driven by cost-effectiveness and the availability of raw materials. This review examines various scientific publications on aqua feed, focusing on both conventional and novel feed formulations and their impact on both small-scale and large-scale aquaculture. Commonly used aqua feed ingredients among African farmers include cassava, maize gluten, groundnut oilcake, sunflower oilcake, soybean meal, kale, peas, garlic, shrimp wastes, and waste blood. Novel ingredients such as insect-based diets, micro-algae, and fish discard formulations are also explored. Aqua feed composition impacts aqua waste, water quality, algae, oxygen demand, fish mortality, and eutrophication, and findings from literature reiterate the need to reorient feed formulation methods and ingredients to achieve a circular economy in Africa. This will entail promoting increased fish production at minimal costs and creating employment while supporting climate adaptation and mitigation efforts. Ultimately, the aqua feed sector has the potential to grow sustainably through the adoption of feed alternatives that prioritize sustainable production and encourage beneficiation studies. Full article

Chinese kale is a native vegetable from the Brassicaceae family that is grown extensively in Southeast Asia and Southern China. Its low genetic transformation and gene editing efficiency hinder gene function research and molecular biology in Chinese kale. CRISPR/Cas9 is a useful tool for plant genome research due to its rapid development and optimization. This study targeted BocPDSs, (BocPDS1, BocPDS2) to establish an effective CRISPR/Cas9 system in Chinese kale. A tandemly arranged tRNA-sgRNA construct was used to express numerous sgRNAs to induce BocPDS1 and BocPDS2 double and single mutations, with a mutation rate of 61.11%. As predicted, several mutant plants showed an albino phenotype with a harbored mutation in an exon and intron region, highlighting the relevance of the intron. The presence of mutations in the intron region suggests that the cleavage process in Chinese kale, utilizing CRISPR/Cas9 shows a preference for AT-rich regions. The distinct and somewhat redundant functions of BocPDS1 and BocPDS2 are demonstrated by the complete albino phenotype of the double mutants and the mosaic albino phenotype of the individual BocPDS1 and BocPDS2 mutants. Specific gene editing modes, including base deletion, base substitution, and base insertion, were identified in the sequence of the target gene. Among them, short nucleotide insertions were the most common type of insertion, with base insertions having the highest frequency (61.54%). Furthermore, no instances of off-target gene editing were detected. The current work demonstrated that the CRISPR/Cas9 gene editing system, which relies on endogenous tRNA processing, can effectively induce mutagenesis in Chinese kale. This finding establishes a theoretical basis and technical backbone for the more effective implementation of CRISPR/Cas9 gene-editing technology in Chinese kale and Brassica plants. Full article