Extended Data Figure 3: Local resolution estimation and combination of maps for model building.

a–c, Local resolution estimation by Resmap of the entire complex I (a), peripheral-arm-focused refinement map (b) and membrane-arm-focused refinement map (c). Maps are coloured according to the shown resolution scale in Å. d. The final map was produced by combining maps with the best local resolution features; that is, for peripheral-arm-focused refinement map (orange), for the distal part of membrane-arm-focused refinement map (green), for 42-kDa subunit map from the selected homogenous complex I class (64 k particles; blue) and the rest of the complex from the best map of the entire complex (magenta).