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Uses of Dalandan and Calamansi

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SIGNIFICANCE OF THE STUDY The student sectors and the reader would be given an extra knowledge about the

role of NATURAL ASTRINGENTS as an acne remover. Every student will be informed about the benefits or advantage of this product compared to the commercial products. The products can help to attain a goal of every people to remove acne The business sector would be enlightened on the use of NATURAL ASTRINGENTS and can be put in a commercial practice and endorsement SCOPE AND LIMITATION This investigatory project was conducted at Aurora Naujan Oriental Mindoro from June 5 to September 10 2012. The same materials were used. This study was limited only in comparative analysis of NATURAL ASTRINGENTS from commercial products. DEFINITION OF TERMS Calamansi- citrus plant/fruit that commonly found in our surroundings. Dalandan- common name for citrus fruit of several trees. This is a hespendium, and its belong to the genus citrus Astringents- any substance such as slum that is used medicinally or industrially or to contract tissues and mucus and other secretion. Alum-any of a group of chemical compounds made up of water molecules and two birds of salts one of which is usually aluminum sulfate , combined in definite proportions.

Acne-eruptive skin disease. It is primaly a disorder of the sebaceous follicles of the skin and appears most often on the face, neck and back. Extraction-method used both commercially and in the laboratory to separate a substance from a mixture or a solution. This is usually done by using a solvent in which it is being extracted or not. Citrus-is a common name for several related ever green trees and shrubs of the rue family, and generally for the fruits they produce. It is plant belongs to the family Rutaceae. Hesperidium-is a cherry with a leathery skin containing oils, and soft segmented pulp. Fruit-seed-bearing structure of a flowering plant. It is a actually ripened ovary, a component of the flowers female reproductive structure. Citric acid-white, solid (COOH)3 soluble in water and slightly soluble in water inorganic solvents, which melts at 153 degree Celsius or 307 degree Fahrenheit

CHAPTER III METHODOLOGY This chapter present the methodology which the researcher applied in undertaking this investigatory project. MATERIALS USED This research is about the NATURAL ASTRINGENTS used a liquid form of dalandan and calamansi in the process of extracting. Bowl serve as a storage of a extracted calamansi and dalandan. And also the stirrer for proper mixing of the product. PROCEDURES Preparing Materials. The researchers prepared the selected materials. Extraction. The researchers get the liquid form of calamansi and dalandan by extracting or separation substance from a solid to liquid. Mixing. The researchers mix all the liquid substance. Replacing. The researchers replace the liquid substance to the plastic bottle.

CHAPTER V SUMMARY OF FINDINGS, CONCLUSION AND RECOMMENDATIONS This chapter presents the summary of findings, conclusion and recommendation, the researchers made after thorough interpretation of data. Findings 1.) To a very high extent, the natural astringents made out of calamansi and dalandan. However, the natural astringent made of calamansi and dalandan are successively avoid the skin disease also known as Acne. 2.) The natural astringent directly affected the skin of face to prevent or reduce oil that cause of having Acne. 3.) There was a significant difference between the fruit growth in experimental and controlled sites. Conclusion 1.) Natural Astringent from the calamansi and dalandan contribute citric acid in fruits. 2.) The greater amount of citric acid from calamansi and dalandan, the higher chance of preventing the oily face. 3.) Fruits from plants need enough fertilizer and good type of soil as habitat for faster and health growth. Recommendation

Based on foregoing, the following were recommended: 1.) Use the natural astringents out of calamansi and dalandan in different types of agent with proper analysis and careful treatment of data. 2.) Conduct comprehensive study or the feasibility of the calamansi and dalandan fruit extract as natural astringent where acne and other skin diseases are contolled. 3.) Proper treating and using of natural astringent to protect the user when using through the help of skin expert or dermatologist.

ABSTRACT Acne is a common skin disease affecting all levels of human society. It may cause insecurity, embarrassment and anxiety in patients. Most treatments for acne are costly and may cause various side effects due to harmful chemicals and ingredients. The study aims to produce a natural astringents from citric acid extracted from dalandan and calamansi that is cheaper, and as effective as those sold in the market. Citric acid was extracted from calamansi and dalandan using the traditional crystallization methodology. The crystals were checked for purity by testing the crystals on potassium carbonate. The citric acid crystals were dissolved in glycerin. Eight different concentrations of citric acid were produced using serial dilution. The pH was taken and found to be ranging from 4-6, which is safe for human skin application. Various concentrations of citric acid (1.25%, 0.625% and 0.3125%) were tested on Staphyloccocus aureus using the Kirby-Bauer method to test the anti-microbial property of citric acid. Growth of inhibition was measured in each of the Petri dishes. A higher concentration was found to eradicate more bacteria. The solutions were then tested on Tetrahymena sp.(protist), to find the safe concentrations when applied to human skin. Results showed that 0.625% and 0.3125% were the safest concentrations for unicellular organism since it took a longer time for these cells to lyse. Citric acid solutions of concentrations 0.625% and 0.3125% were tested on the skin of four adult female albino rabbits. A grade of 0, according to the Environmental Protection Agency(EPA) standard scale for dermal irritation testing, resulted from the animal testing part of

the experiment. This grade signified that no sign of dermal irritation was observed. No manifestation of redness or formation of comedones was found after 30 minutes to 3 days of application of the product.

Diagrams of the Procedure Preparing the raw materials Extracting the liquid from the fruit

Mixing all the Replace the liquid liquids to the bottle

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