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    HPLC Simulator

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    Erin Love
    The document provides instructions for an HPLC simulator assignment due November 20th. Students are asked to run 5 experiments using different solvents and conditions to separate 5 compounds, and report their results including an explanation of why their conditions worked best. They are also asked to investigate the effects of temperature on one run and calculate resolution between two barely separated peaks using three different methods.

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    HPLC Simulator

    Uploaded by

    Erin Love
    155 views1 page
    The document provides instructions for an HPLC simulator assignment due November 20th. Students are asked to run 5 experiments using different solvents and conditions to separate 5 compounds, and report their results including an explanation of why their conditions worked best. They are also asked to investigate the effects of temperature on one run and calculate resolution between two barely separated peaks using three different methods.

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    guide on learning how to use the online HPLC simulator with sample assignment

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    The document provides instructions for an HPLC simulator assignment due November 20th. Students are asked to run 5 experiments using different solvents and conditions to separate 5 compounds, and report their results including an explanation of why their conditions worked best. They are also asked to investigate the effects of temperature on one run and calculate resolution between two barely separated peaks using three different methods.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    155 views1 page

    HPLC Simulator

    Uploaded by

    Erin Love
    The document provides instructions for an HPLC simulator assignment due November 20th. Students are asked to run 5 experiments using different solvents and conditions to separate 5 compounds, and report their results including an explanation of why their conditions worked best. They are also asked to investigate the effects of temperature on one run and calculate resolution between two barely separated peaks using three different methods.

    Copyright:

    © All Rights Reserved
    Download as DOCX, PDF, TXT or read online from Scribd
    Download as docx, pdf, or txt
    of 1

    Chem 325

    HPLC Simulator
    20, by noon

    Fall 2015
    Due: November

    Instructions: Download (or run online) HPLC simulator @ hplcsimulator.org

    Initial Settings:
    Column = Agilent Zorbax SB-C18
    Flow rate = 1 mL/min
    Temperature = 25C.
    Your goal: to separate the five compounds listed in the table on the lower right with the best
    results (all five peaks resolved and k in the range 2-10 if possible). Report your conditions, with
    an explanation, for the following five runs (no credit will be given without an appropriate
    explanation). Suitable explanations will include why the particular condition worked best, and
    comparison to other conditions (i.e. why is the %B different for ACN vs. MeOH?).
    1) Use water (A) /methanol (B) and isocratic elution (vary % B via a sliding bar on top left)
    2) Use water (A) /acetonitrile (B) and isocratic elution (vary % B)
    3) Use water (A) /methanol or acetonitrile (B) and gradient elution (vary % B over some
    time, play with time settings)
    4) For one of the isocratic runs investigate the effects of temperature (sliding bar on middle
    left)
    Record typical HETP, N, k (range)
    Lastly, choose a setting where two (any) peaks elute close together and are barely separated.
    Using the data for these two peaks, calculate resolution using THREE methods (see eq-n
    below). Hint: you have to estimate (measure) the width of the peak (w) which is best done by
    zooming into the chromatogram.

    Rs

    1 k 2'
    (tr )
    N


    1 k a' v e
    w a v e 4 - 1

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