Biosafety Issues and BT Cotton - A Case Study: O. P. Govila Retd. Professor Genetics Iari

Biosafety Issues and Bt cotton A case study
O. P. GOVILA Retd. Professor Genetics IARI
Introduction
Classical plant breeding is limited to the introduction of required characters into plant by genetic crossing during sexual reproduction To feed the ever increasing population more and more food has to be produced from less and less land water and other natural resources It is obvious that emphasis has to be laid on new technologies that can improve crop yield against adverse conditions of biotic and abiotic stresses and improve the quality
contd.
The earlier part of plant biotechnology era has been the development of tissue culture protocols of several commercially important crops The gene revolution as compared to green revolution is poised to benefit both poor and rich farmers equally and has an immense potential in transforming global agriculture. In India, significant efforts have been made to significantly for developi several programmes in Biotechnology
WHAT IS A TRANSGENIC CROP A transgenic crop is a crop which contains a gene or genes of a different species artificially inserted in its genome,which may come from an unrelated plant or from a completed different species.
Why make transgenic crops?

Due to limitations of conventional breeding for attaining the desirable traits use of recombinant DNA technology has been taken advantage of and development of transgenics started
CELL: SMALLEST UNIT OF LIFE NUCLEUS: BRAIN OF THE CELL
CHROMOSOMES: STRUCTURE CONTAINING THE GENETIC INFORMATION
GENES: SMALLER PIECES OF GENETIC INFORMATION
DNA: BUILDING BLOCK OF THE GENE, DOUBLE HELIX STRUCTURE
Organization of DNA in the cell
Biotechnology is an Extension of Traditional Plant Breeding

TRADITIONAL PLANT BREEDING
DNA is a strand of genes, much like a strand of pearls. Traditional plant breeding combines many genes at once.
Many genes are transferred Desired Gene
X
Donor Plant Commercial Plant Variety New Plant Variety A single gene is transferred
PLANT BIOTECHNOLOGY
Using plant biotechnology, you can add a single gene to the strand. Desired Gene
Desired Gene
+
Donor Commercial Plant Variety Improved Commercial Plant Variety
Development of GM Crop/ Trasgenics

Identify gene(s) giving a desired trait Make copies of the gene Transfer to plant tissue Regenerate plants Lab analysis and safety testing Development of a variety Field tests Approval by Government agencies Commercialization Monitoring of efficacy and safety
7-8 years
Methods of producing transgenic plant
Advantages of transgenic crops over breeding

Breeding Exchange of genes within a species Gene of interest with flanking sequence transferred Simple Technology History of Safety Transgenic crops No barrier Only Gene of Interest
Intensive Technology New Technology Time needed for Safety History
Transgenic Crops: Development Objectives

Integrated pest management Herbicide tolerance Nutritional enhancements Product quality improvement Increase in yield Stress tolerance Plant based pharmaceuticals
GLOBAL SCENARIO 2005

First crop introduced was Flavr Savr tomato in USA in 1995 So far 19 crops approved for commercial cultivation Include canola, carnation, chicory, cotton, linseed, green pepper, maize, melon, potato, rice, soybean, squash, sugarbeet, sunflower, tobacco and tomato Major characteristics are insect resistance, herbicide tolerance, virus resistance and improved product quality

Major countries include USA, Canada, Japan, China, EU, Argentina, South Africa Only four crops being marketed commercially I.e., corn, cotton, soybean and canola Commercial production initiated for papaya, squash and tobacco in USA Others are approved but not yet being marketed

Area under cultivation: 90 million hectares No. of countries: 21 No. of farmers: 8.25 million Crops: Corn, soybean, cotton and canola Transgenic traits: insect resistance & herbicide tolerance
Global area of transgenic crops from 1996 to 2005 (million hectares)
90.0 100 81.0 67.7 80 52.658.7 60 39.944.2 27.8 40 20 1.7 11.0 0
Series1
96
97
98
99
00
01
02
03
04
19
19
19
19
20
20
20
20
20
20
05
RESEARCH & DEVELOPMENT STATUS

63 countries involved at various stages 57 plants identified for development Second generation of traits include modifications such as nutritional enhancement, disease tolerance, stress tolerance and production of pharmaceuticals
COMMERCIAL USE IN INDIA

Only one crop approved i.e. Bt cotton Three hybrids containing Cry1Ac gene approved in 2002 and one in 2004 62 hybrids approved for Kharif 2006 Three new events approved I.e. i. Cry 1Ac gene (event 1) byM/s J.K.Agri Seeds Ltd. ii. Fusion genes (cry 1Ab+cry 1Ac) 'GFMby M/s Nath Seeds iii. Stacked genes cry1Ac and cry1Ab byM/sMahyco
PRE-Bt COTTON PRODUCTION & Bt DEVELOPMENT

Area, Production, Productivity of the decade prior to Bt introduction Productivity of Rain-fed vs Irrigated Cotton pre-Bt period Indian Cotton Vs International Cotton Sequence of Development of Bt Cotton Economics of first generation Bt Cotton in country-wide coordinated trials
Bt Cotton Development Process in India
BIOSAFETY ASSESSMENT
STUDIES ON ENVIRONMENTAL SAFETY STUDIES ON FOOD SAFETY RISK ASSESSMENT
1. STUDIES ON ENVIRONMENTAL SAFETY

POLLEN ESCAPE/OUT CROSSING: MULTI LOCATION EXPERIMENTS WERE CONDUCTED IN 1996, 1997 AND 2000 Due to stickiness pollen travel is limited No chance of transfer from tetraploid Bt hybrids to cultivated diploid species No wild species occur in India
AGGRESSIVENESS AND WEEDINESS TO ASSESS THE WEEDINESS OF BT COTTON, THE RATE OF GERMINATION AND VIGOUR WERE COMPARED WITH NON BT COUNTERPARTS No significant differences between Bt and non Bt cotton for germination and vigor Hence there is no difference between Bt and non Bt cotton with regard to their weediness and aggressiveness potential
EFFECT OF Bt ON NON TARGET ORGANISMS STUDIES CONDUCTED DURING THE MULTI LOCATION FIELD TRIALS Bt cotton hybrids did not have any TOXIC effects on non target species such as: Sucking pest (aphids, jassids , whitefly and mites) The beneficial insects (ladybird, beetle, honeybees and spiders) remained active in both Bt and Non Bt cotton hybrids.
PRESENCE OF Bt PROTEIN IN SOIL STUDIES WERE CONDUCTED TO ASSESS THE POSSIBLE RISK OF ACCUMULATION OF BT PROTEIN IN THE SOIL Bt protein was not detected in soil samples indicating that Cry1Ac protein was rapidly degraded in the soil The half-life of the Cry1Ac protein in plant tissues was calculated to be 41 days which is comparable to the degradation rates reported for microbial formulations of Bt.
EFFECT OF BT PROTEIN ON SOIL MICROFLORA STUDIES WERE CONDUCTED TO EVALUATE ANY IMPACT OF BT PROTEIN LEACHED BY ROOTS OF BT COTTON ON THE SOIL MICROFLORA Results showed that there was no significant difference in population of microbes and soil invertebrates ( like earthworms) between Bt and non Bt samples
2. STUDIES ON FOOD SAFETY

FOR EVALUATING FOOD SAFETY, THE STUDIES INCLUDED: Compositional analysis Allergenicity studies Toxicological studies Presence of Bt protein in cotton seed oil Feeding studies on cows, buffaloes, poultry and fish.
COMPOSITIONAL ANALYSIS
STUDIES REVEALED THAT THERE WAS NO CHANGE IN THE COMPOSITION IN THE Bt AND NON Bt COTTON SEEDS WITH RESPECT TO: Proteins Carbohydrates Oil Calories Ash content
ALLERGENICITY STUDIES
ALLERGENICITY STUDIES WERE CONDUCTED ON BROWN NORWAY RATS BY FEEDING Bt AND NON Bt SEEDS. RESULTS SHOWED THAT: No significant difference in feed consumption, weight gain and general health There was no significant change in endogenous allergens of Bt seed compared to non Bt cotton seed
TOXICOLOGICAL STUDIES
GOAT FEEDING STUDY WAS CONDUCTED FOR UNDERSTANDING THE TOXICOLOGICAL EFFECTS OF Bt COTTON SEED. THE ANIMALS WERE ASSESSED FOR GROSS PATHOLOGY AND HISTOPATHOLOGY: No significant differences were found between animals fed with Bt and non Bt cotton seed
PRESENCE OF Cry1Ac PROTEIN IN Bt COTTON SEED OIL

STUDIES HAVE INDICATED THAT Cry1Ac PROTEIN WAS NO FOUND IN REFINED OIL OBTAINED FROM Bt COTTON SEEDS
FEEDING STUDIES ON COWS, BUFFALOES, POULTRY AND FISH

The feeding experiments using Bt cotton seed meal were conducted at National Dairy Research Institute, Karnal on lactating cows; Department of Animal Nutrition, College of Veterinary Sciences, G.B. Pant University of Agriculture & Technology, Pantnagar on lactating buffaloes. Central Avian Research Institute, Izatnagar on poultry Central Institute on Fisheries Education, Mumbai on fish These experiments indicated that Bt cotton seed meal was nutritionally as wholesome and safe as the non Bt cotton seed meal.
3. RISK MANAGEMENT
PEST POPULATION EXPOSED TO Bt CROPS CONTINUOUSLY FOR SEVERAL YEARS MAY DEVELOP RESISTANCE TO THE Bt TOXINS THROUGH NATURAL SELECTION MUTATION, AND SELECTION :
To prevent resistance build up it is recommended to plant sufficient non Bt cotton (20%) to serve as a refuge for Bt susceptibility in seeds The refuge strategy is designed to ensure that Bt susceptible insects will be available to mate with Bt resistant insects, should they arise. Available genetic data indicates that susceptibility is dominant over resistance. Therefore, the offspring of these matings would most likely be Bt susceptible, thus mitigating the spread of resistance in the
4. OTHERS SAFEGUARDS
CONFIRMATION OF THE ABSENCE OF TERMINATOR TEHNOLOGY Molecular detection test in the Bt cotton hybrids was performed for absence of any terminator gene.
5. CONDITIONS STIPULATED BY GEAC

Valid for three years: April 02 to March, 05 Three hybrids namely MECH12Bt, MECH162Bt
and MECH184Bt Provide same non Bt seed to meet refuge requirements Conduct studies to monitor resistance development Provide information to government on distribution of the seed through its dealers and agents Labeling requirements such as GEAC number, etc., Develop Bt based IPM program Undertake education and awareness program Meet other requirements as stipulated
State-wise Adoption of Bt-Cotton (000ha)

State Andhra Pradesh Karnataka Tamil Nadu Maharashtra Gujarat* Madhya Pradesh Punjab Haryana Rajasthan 2002 3.8 2.2 0.4 12.4 9.1 1.5 0 0 0 2003 5.2 3.6 3.4 18.7 42.1 13.0 0 0 0 2004 74.9 20.5 9.8 208.7 134.0 87.9 0 0 0 2005 226.7 28.9 18.4 621.1 147.4 142.1 51.4 13.1 1.6 % Growth 597 1214 4500 4909 1520 9373 ---% area covered in 2005 22.9 08.0 07.8 20.9 07.2 22.6 08.9 02.2 00.4
Area under Bt cotton cultivation in India
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Biosafety Issues and Bt cotton A case study

O. P. GOVILA Retd. Professor Genetics IARI

Why make transgenic crops?

CELL: SMALLEST UNIT OF LIFE NUCLEUS: BRAIN OF THE CELL

CHROMOSOMES: STRUCTURE CONTAINING THE GENETIC INFORMATION

GENES: SMALLER PIECES OF GENETIC INFORMATION

DNA: BUILDING BLOCK OF THE GENE, DOUBLE HELIX STRUCTURE

Organization of DNA in the cell

Biotechnology is an Extension of Traditional Plant Breeding

Development of GM Crop/ Trasgenics

Methods of producing transgenic plant

Advantages of transgenic crops over breeding

Intensive Technology New Technology Time needed for Safety History

Transgenic Crops: Development Objectives

GLOBAL SCENARIO 2005

GLOBAL SCENARIO 2005

GLOBAL SCENARIO 2005

Global area of transgenic crops from 1996 to 2005 (million hectares)

90.0 100 81.0 67.7 80 52.658.7 60 39.944.2 27.8 40 20 1.7 11.0 0

RESEARCH & DEVELOPMENT STATUS

COMMERCIAL USE IN INDIA

PRE-Bt COTTON PRODUCTION & Bt DEVELOPMENT

Bt Cotton Development Process in India

1. STUDIES ON ENVIRONMENTAL SAFETY

2. STUDIES ON FOOD SAFETY

PRESENCE OF Cry1Ac PROTEIN IN Bt COTTON SEED OIL

FEEDING STUDIES ON COWS, BUFFALOES, POULTRY AND FISH

5. CONDITIONS STIPULATED BY GEAC

State-wise Adoption of Bt-Cotton (000ha)

Area under Bt cotton cultivation in India

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