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    Ion Chromatography

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    alexpharm
    Ion chromatography uses ion-exchange resins to separate atomic or molecular ions based on their interaction with the resin. The column packings consist of ion-exchange resins bonded to inert polymeric particles. For cation separation the resin is usually a sulfonic or carboxylic acid, and for anion separation the resin is usually a quaternary ammonium group. Ions are detected by measuring the conductivity of the solution, but an eluent suppressor is used to selectively remove mobile phase ions after the analytical column and before detection to reduce background conductivity.

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    Ion Chromatography

    Uploaded by

    alexpharm
    163 views2 pages
    Ion chromatography uses ion-exchange resins to separate atomic or molecular ions based on their interaction with the resin. The column packings consist of ion-exchange resins bonded to inert polymeric particles. For cation separation the resin is usually a sulfonic or carboxylic acid, and for anion separation the resin is usually a quaternary ammonium group. Ions are detected by measuring the conductivity of the solution, but an eluent suppressor is used to selectively remove mobile phase ions after the analytical column and before detection to reduce background conductivity.

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    Ion chromatography uses ion-exchange resins to separate atomic or molecular ions based on their interaction with the resin. The column packings consist of ion-exchange resins bonded to inert polymeric particles. For cation separation the resin is usually a sulfonic or carboxylic acid, and for anion separation the resin is usually a quaternary ammonium group. Ions are detected by measuring the conductivity of the solution, but an eluent suppressor is used to selectively remove mobile phase ions after the analytical column and before detection to reduce background conductivity.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    163 views2 pages

    Ion Chromatography

    Uploaded by

    alexpharm
    Ion chromatography uses ion-exchange resins to separate atomic or molecular ions based on their interaction with the resin. The column packings consist of ion-exchange resins bonded to inert polymeric particles. For cation separation the resin is usually a sulfonic or carboxylic acid, and for anion separation the resin is usually a quaternary ammonium group. Ions are detected by measuring the conductivity of the solution, but an eluent suppressor is used to selectively remove mobile phase ions after the analytical column and before detection to reduce background conductivity.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as DOC, PDF, TXT or read online from Scribd
    Download as doc, pdf, or txt
    of 2

    Ion Chromatography

    Introduction

    Ion chromatography is a form of liquid chromatography that uses ion-exchange resins to


    separate atomic or molecular ions based on their interaction with the resin. Its greatest
    utility is for analysis of anions for which there are no other rapid analytical methods. It is
    also commonly used for cations and biochemical species such as amino acids and
    proteins. Most ion-exchange separations are done with pumps and metal columns, see the
    HPLC document for typical instrumentation. Simple LC columns are used to separate one
    ion from others in ultra-trace analytical applications.

    Ion-Exchange Resins

    The column packings for ion chromatography consist of ion-exchange resins bonded to
    inert polymeric particles (typically 10 µm diameter). For cation separation the cation-
    exchange resin is usually a sulfonic or carboxylic acid, and for anion separation the
    anion-exchange resin is usually a quaternary ammonium group. For cation-exchange with
    a sulfonic acid group the reaction is:

    -SO3- H+(s) + Mx+(aq) <===> -SO3- Mx+(s) + H+(aq)

    where Mx+ is a cation of charge x, (s) indicates the solid or stationary phase, and (aq)
    indicates the aqueous or mobile phase. The equilibrium constant for this reaction is:

    [-SO3- Mx+]s [H+]aq


    Keq = --------------------
    [-SO3- H+]s [Mx+]aq

    Different cations have different values of Keq and are therefore retained on the column for
    different lengths of time. The time at which a given cation elutes from the column can be
    controlled by adjusting the pH ([H+]aq). Most ion-chromatography instruments use two
    mobile phase reservoirs containing buffers of different pH, and a programmable pump
    that can change the pH of the mobile phase during the separation.

    Ion Detection

    Ions in solution can be detected by measuring the conductivity of the solution. In ion
    chromatography, the mobile phase contains ions that create a background conductivity,
    making it difficult to measure the conductivity due only to the analyte ions as they exit
    the column. This problem can be greatly reduced by selectively removing the mobile
    phase ions after the analytical column and before the detector. This is done by converting
    the mobile phase ions to a neutral form or removing them with an eluent suppressor,
    which consists of an ion-exchange column or membrane. For cation analysis, the mobile
    phase is often HCl or HNO3, which can be neutralized by an eluent suppressor that
    supplies OH-. The Cl- or NO3- is either retained or removed by the suppressor column or
    membrane. The same principle holds for anion analysis. The mobile phase is often NaOH
    or NaHCO3, and the eluent suppressor supplies H+ to neutralize the anion and retain or
    remove the Na+.

    Molecular ions, such as proteins, that have absorption bands in the ultraviolet or visible
    spectral region can be detected by absorption spectroscopy.

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