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    DNA Extraction

    Uploaded by

    Kiko
    The document provides instructions for extracting DNA from green split peas. It describes blending split peas with salt and water, mixing the blended solution with detergent, adding enzymes, and separating the DNA from other materials by layering alcohol on top and collecting stringy DNA precipitate.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd

    DNA Extraction

    Uploaded by

    Kiko
    1K views4 pages
    The document provides instructions for extracting DNA from green split peas. It describes blending split peas with salt and water, mixing the blended solution with detergent, adding enzymes, and separating the DNA from other materials by layering alcohol on top and collecting stringy DNA precipitate.

    Original Description:

    How to collect DNA from almost anything alive, better than watching TV for your kids.

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    © Attribution Non-Commercial (BY-NC)

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    The document provides instructions for extracting DNA from green split peas. It describes blending split peas with salt and water, mixing the blended solution with detergent, adding enzymes, and separating the DNA from other materials by layering alcohol on top and collecting stringy DNA precipitate.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    1K views4 pages

    DNA Extraction

    Uploaded by

    Kiko
    The document provides instructions for extracting DNA from green split peas. It describes blending split peas with salt and water, mixing the blended solution with detergent, adding enzymes, and separating the DNA from other materials by layering alcohol on top and collecting stringy DNA precipitate.

    Copyright:

    Attribution Non-Commercial (BY-NC)
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    of 4

    Print-and-Go™

    http://learn.genetics.utah.edu

    First, you need to find something that contains DNA. Since DNA is the blueprint for life,
    everything living contains DNA. For this experiment, we like to use green split peas. But there
    are lots of other DNA sources too, such as:
    • Spinach
    • Chicken liver Start
    • Strawberries
    • Broccoli

    Certain sources of DNA should not be used,


    such as:
    • Your family pet, Fido the dog
    • Your little sister’s big toe
    • Bugs you caught in the yard

    Step 1 Blender Insanity!


    Put in a blender:
    • 1/2 cup of split peas (100ml)
    • 1/8 teaspoon table salt (less than 1ml)
    • 1 cup cold water (200ml)

    Blend on high for 15 seconds.


    The blender separates the pea cells from
    each other, so you now have a really thin
    pea-cell soup.

    © 2008 University of Utah This activity was downloaded from: http://learn.genetics.utah.edu


    Print-and-Go™
    http://learn.genetics.utah.edu

    How to Extract DNA from Anything Living

    Soapy Peas Step 2


    Pour your thin pea-cell soup through a
    strainer into another container (like a
    measuring cup).
    Add 2 tablespoons liquid detergent (about
    30ml) and swirl to mix.
    Let the mixture sit for 5-10 minutes.
    Pour the mixture into test tubes or other
    small glass containers, each about 1/3 full.

    Step 3 Enzyme Power


    Add a pinch of enzymes to each test tube
    and stir gently. Be careful! If you stir too
    hard, you’ll break up the DNA, making it
    harder to see.
    Use meat tenderizer for enzymes. If you
    can’t find tenderizer, try using pineapple
    juice or contact lens cleaning solution.

    © 2008 University of Utah This activity was downloaded from: http://learn.genetics.utah.edu 2


    Print-and-Go™
    http://learn.genetics.utah.edu

    How to Extract DNA from Anything Living

    Alcohol Separation Step 4


    Tilt your test tube and slowly pour
    rubbing alcohol (70-95% isopropyl or
    ethyl alcohol) into the tube down the side
    so that it forms a layer on top of the pea
    mixture. Pour until you have about the
    same amount of alcohol in the tube as
    pea mixture.
    Alcohol is less dense than water, so it
    floats on top. Look for clumps of white
    stringy stuff where the water and alcohol
    layers meet.

    Finish What is that Stringy Stuff?


    DNA is a long, stringy molecule. The salt
    that you added in step one helps it stick
    together. So what you see are clumps of
    tangled DNA molecules!
    DNA normally stays dissolved in water,
    but when salty DNA comes in contact with
    alcohol it becomes undissolved. This is
    called precipitation. The physical force of
    the DNA clumping together as it precipitates
    pulls more strands along with it as it rises
    into the alcohol.
    You can use a wooden stick or a straw to
    collect the DNA. If you want to save your
    DNA, you can transfer it to a small container
    filled with alcohol.

    © 2008 University of Utah This activity was downloaded from: http://learn.genetics.utah.edu 3


    Print-and-Go™
    http://learn.genetics.utah.edu

    How to Extract DNA from Anything Living

    You Have Just Completed DNA Extraction!


    Now that you’ve successfully extracted DNA from one source, you’re ready to
    experiment further. Try these ideas or some of your own:

    Experiment with other DNA sources. Which


    source gives you the most DNA? How can you
    compare them?

    Experiment with different soaps and


    detergents. Do powdered soaps work as well as
    liquid detergents? How about shampoo or body
    scrub?

    Experiment with leaving out or changing steps.


    We’ve told you that you need each step, but
    is this true? Find out for yourself. Try leaving
    out a step or changing how much of each Want to conduct more DNA extraction
    ingredient you use. experiments? Try out different soaps and
    detergents. Do powdered soaps work as well
    Do only living organisms contain DNA? Try as liquid detergents?
    extracting DNA from things that you think
    might not have DNA.

    Supported by a Science Education Partnership Award (SEPA) [No. 1 R25 RR16291-01] from the
    National Center for Research Resources, a component of the National Institutes of Health, Depart-
    ment of Health and Human Services. The contents provided here are solely the responsibility of the
    authors and do not necessarily represent the official views of NCRR or NIH.

    © 2008 University of Utah This activity was downloaded from: http://learn.genetics.utah.edu 4

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