Czapek Dox Media Oxoid Product Detail

10/28/2014
CM0097, Czapek Dox Media | Oxoid - Product Detail
Dehydrated Culture Media
CZAPEK
DOX AGAR
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(MODIFIED)
Code: CM0097
a solid defined medium for the cultivation of those fungi and bacteria which are able to utilise sodium
nitrate as the sole source of nitrogen. The acidity of the medium may be increased for the cultivation
of acidophilic organisms such as yeasts.
Typical Formula*
gm/litre
Sodium nitrate
2.0
Potassium chloride
0.5
Magnesium glycerophosphate
0.5
Ferrous sulphate
0.01
Potassium sulphate
0.35
Sucrose
30.0
Agar
12.0
pH 6.8 0.2 @ 25C
* Adjusted as required to meet performance standards
Directions
Suspend 45.4g in 1 litre of distilled water. Bring to the boil to dissolve completely. Sterilise by
autoclaving at 121C for 15 minutes. Mix well before pouring. If required, adjust the reaction to pH 3.5
0.2 by adding 10ml of Lactic Acid 10% (SR0021) per litre after sterilisation.
Description
Czapek Dox Agar (Modified) is a medium containing sodium nitrate as the sole source of nitrogen, it
is one of the most useful solid media for the general cultivation of fungi.
In the Oxoid medium magnesium glycerophosphate and potassium sulphate replace the magnesium
sulphate and potassium phosphate of the original. This modification prevents the precipitation of
magnesium phosphate. The medium is also a highly satisfactory substrate for chlamydospore
production by Candida albicans 1.
Dawson1 employed Oxoid Czapek Dox Agar (Modified) in her technique for the identification of
Candida albicans by chlamydospore formation in primary culture, using swabs taken from the mouth
and from the vagina. Identification was usually possible within 24 hours. The Oxoid medium showed
good chlamydospore production whereas the original formulation did not. After 24 hours incubation 23
out of 27 Candida albicans strains had formed chlamydospores on Oxoid Czapek Dox Agar (Modified),
21 on rice infusion agar, 10 on Oxoid Corn Meal Agar (CM0103) and 10 on a corn meal agar made in
the laboratory. After 48 hours 25 strains had formed chlamydospores on both the Oxoid medium and
the rice agar, 24 on Oxoid Corn Meal Agar and 20 on the laboratory medium. Dawson concluded that
the Oxoid Czapek Dox medium and the rice infusion agar were the most satisfactory media. None of
14 strains of unidentified yeasts formed chlamydospores on any medium.
Smith2 cited the following recommendations for the use of Czapek Dox Agar for taxonomic studies:
by Thom and Church3 for Aspergillus; by Thom4 and by Raper and Thom5 for Penicillium; and by
Wakesman6 for actinomycetes.
Technique
General Cultivation
To avoid excessive condensation cool the molten medium to 50C before pouring approximately 12ml
into each 9cm diameter Petri dish. Store the poured plates in an inverted position and inoculate using
a needle or wire, with the plate still inverted in order to avoid scattering stray fungal spores over the
surface of the medium. Time and temperature of incubation vary considerably according to the
species being cultivated. As a general guide, incubate for 1-2 weeks at 25C. Most Penicillium
species have an optimum growth temperature between 20 and 25C, whilst many Aspergillus species
grow best at about 30C. However, different fungi grow over a wide range of temperatures;
Aspergillus fumigatus grows well at 50C (Smith2) and Cladosporium herbarum will grow on meat at
-6C 7,8.
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CM0097, Czapek Dox Media | Oxoid - Product Detail
Identification of Candida albicans 1

1. Using an inoculating needle (previously flamed, cooled and rubbed against the swab) cut
across and through the medium in a Czapek Dox Agar plate to the base of the petri dish. With
the same needle, raise the medium along the whole of one side of the cut - so that the
inoculum is spread between the agar and the base of the dish.
2. Incubate the inoculated plates for 24 hours at 28C.
3. Using a low-power objective, microscopically examine the unopened plates for chlamydospores
through the base of each dish. Alternatively, remove the tops of the dishes, and examine
through the top of the medium.
4. If no chlamydospores are seen, incubate for a further 24 hours and re-examine.
Storage conditions and Shelf life
Store the dehydrated medium at 10-30C and use before the expiry date on the label.
Store the prepared agar plates at 2-8C.
Appearance
Dehydrated medium: White coloured, free-flowing powder
Prepared medium: Off-white coloured gel
Quality control
Positive control:
Expected results
Aspergillus niger ATCC 9642 * White / yellow mycelium, black spores
Candida albicans ATCC 10231 *
Good growth; cream coloured colonies
Negative control:
Uninoculated medium
No change
* This organism is available as a Culti-Loop
References
1. Dawson Christine O. (1962) Saboutaudia 1. 214-219
2. Smith G. (1960) An Introduction to Industrial Mycoloogy 5th ed., Edward Arnold Ltd., London.
3. Thom C. and Church M. B. (1926) The Aspergilli Williams and Wilkins Co. Baltimore.
4. Thom C. (1930) The Aspergilli Williams and Wilkins Co. Baltimore.
5. Raper K. B. and Thom C. (1949) Manual of the Penicillia Williams and Wilkins Co. Baltimore.
6. Wakesman S. A. (1931) Principals of soil Microbiology Bailliere Tindall and Cox, London.
7. Brooks F. T. and Kidd M. N. (1921) Specia. Report No 6, Food Invest. Board, DSIR, London.
8. Brooks F. T. and Handsford C. G. (1922) Trans. Brit. Mycol. Soc. 8. 113-142.
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10/28/2014

CM0097, Czapek Dox Media | Oxoid - Product Detail

Dehydrated Culture Media

* Adjusted as required to meet performance standards

CM0097, Czapek Dox Media | Oxoid - Product Detail

Identification of Candida albicans 1

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