Corn Meal Agar: Intended Use

Corn Meal Agar M146
Intended use
Corn Meal Agar is recommended for chlamydospore production by Candida albicans and the maintenance of fungal stock
cultures.
Composition**
Ingredients Gms / Litre
Corn meal, infusion from 50.000
Agar 15.000
Final pH ( at 25°C) 6.0±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 17 grams in 1000 ml distilled water. Heat to boiling to dissolve the medium completely. If desired add 1% polysorbate
80. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 45-50°C. Mix well and pour into sterile Petri
plates.
Principle And Interpretation

Chlamydospore production is an accepted criterion for the identification of Candida species. Corn Meal Agar is a well-
established mycological medium used for the cultivation of fungi and to study chlamydospores production of Candida
species (1). Corn Meal Agar is a general purpose medium used for the cultivation of fungi and for the study of Candida
species for chlamydospore production. Pollack and Benham (1) have described the usefulness of this medium for studying the
morphology of Candida. Walker and Huppert (2) modified this medium by adding polysorbate 80, which then stimulated
faster and plenty of chlamydospore formation of Candida species.
This is a very simple formulation containing only cornmeal infusion and agar. However this infusion has enough nutrients to
enhance the growth of fungi. Polysorbate 80 is a mixture of oleic esters, which activates the production of chlamydospore by
Candida albicans, Candida stellatoides and Candida tropicalis (3). Some Candida species lose their ability of chlamydospore
formation by repeated sub culturing.
Pick a suspected colony from Sabouraud Dextrose Agar (M063) using a straight wire, and make a deep cut in the Corn Meal
Agar plate. Repeat for each colony. Place a flamed sterile coverslip over the line of inoculum. After incubation for 24-48
hours at 25-30°C, the streaks are examined microscopically, through the coverslip, using low and high power objectives.
C.albicans produces mycelium bearing ball-like clusters of budding cells and characteristics thick walled round
chlamydospores (4).
Type of specimen
Food and dairy samples
Specimen Collection and Handling:

For food and dairy samples, follow appropriate techniques for sample collection and processing as per guidelines (7,8).
After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions :
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection.
Follow good microbiological lab practices while handling specimens and culture. Standard precautions as per
established guidleines should be followed while handling specimens. Saftey guidelines may be referred in
individual safety data sheets
Limitations :
Further biochemical tests must be carried out for confirmation.
Performance and Evaluation
Performance of the medium is expected when used as per the direction on the label within the expiry period when stored at
recommended temperature.
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
Quality Control
Appearance
Cream to yellow coarse free flowing powder
Gelling
Firm, comparable with 1.5% Agar gel
Colour and Clarity of prepared medium
Light amber coloured, opalescent gel forms in Petri plates
Reaction
Reaction of 1.7% w/v aqueous solution at 25°C. pH : 6.0±0.2
pH
5.80-6.20
Cultural Response
M146: Cultural characteristics observed after an incubation at 23-27°C for upto 4 days.
Organism Inoculum Growth Chlamydospores Recovery
(CFU)
Aspergillus brasiliensis 50-100 luxuriant negative
ATCC 16404
Candida albicans ATCC 50-100 luxuriant positive >=70%
10231 (00054*)
Saccharomyces cerevisiae 50-100 luxuriant negative >=70%
ATCC 9763 (00058*)
Saccharomyces uvarum 50-100 luxuriant negative >=70%
ATCC 28098
Storage and Shelf Life

Store between 10-30°C in a tightly closed container and the prepared medium at 20-30°C. Use before expiry date on
the label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump
formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation.
Store in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly
after use. Use before expiry date on the label.
Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical
sample must be decontaminated and disposed of in accordance with current laboratory techniques (5,6).
Reference
1. Pollack and Benham, 1960, J. Lab. Clin. Med., 50:313.
2. Walker and Huppert, 1960, Tech. Bull. Reg. Med. Technol., 30:10.
3. Cooper and Silvo-Hunter, 1985, Manual of Clinical Microbiology, Lennette, Balows, Hausler and Shadomy (Eds.), 4th ed.,
ASM, Washington, D.C.
4. Conant N. F., Smith D. T., Baker R. D., Callaway J. L. and Martin D. S., 1971, Manual of Clinical Mycology, 3rd Ed., USA
5.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.
6. Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
Manual of Clinical Microbiology, 11th Edition. Vol. 1.
7. American Public Health Association, Standard Methods for the Examination of Dairy Products, 1978, 14th Ed., Washington
D.C.
8. Salfinger Y., and Tortorello M.L. Fifth (Ed.), 2015, Compendium of Methods for the Microbiological Examination of
Foods, 5th Ed., American Public Health Association, Washington, D.C.
Revision : 03/2018
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate office :
A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:
techhelp@himedialabs.com Website: www.himedialabs.com

Corn Meal Agar: Intended Use

Uploaded by

Copyright:

Available Formats

Corn Meal Agar: Intended Use

Uploaded by

Document Information

Original Title

Copyright

Available Formats

Share this document

Share or Embed Document

Sharing Options

Did you find this document useful?

Is this content inappropriate?

Copyright:

Available Formats

Corn Meal Agar: Intended Use

Uploaded by

Copyright:

Available Formats

Corn Meal Agar M146

Principle And Interpretation

Specimen Collection and Handling:

Storage and Shelf Life

You might also like