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    RM188

    Uploaded by

    ShahinuzzamanAda

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd

    RM188

    Uploaded by

    ShahinuzzamanAda
    0 views2 pages

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    © © All Rights Reserved

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    RM188

    Uploaded by

    ShahinuzzamanAda

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    © All Rights Reserved
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    of 2

    BHI Powder RM188

    Intended use
    BHI Powder is a highly nutritious ingredient, employed in number of media, for cultivation of fastidious microorganisms.
    such as in BHI Agar/Broth, SABHI Agar Base, etc. to grow Staphylococci, Streptococci, Haemophilus and
    Neisseria species and pathogenic fungi. It is a yellowish brown coloured, free flowing powder having characteristic but
    not putrescent odour.

    Warning and Precautions


    Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face protection.
    Follow good microbiological lab practices while handling specimens and culture. Safety guidelines may be referred in
    individual safety data sheets.

    Limitations
    1. It is biological origin product since variation in colour of powder and clarity may observed.
    2. Each lot of the product has been tested for the organisms specified on the COA. It is recommended to users to validate
    the medium for any specific microorganism other than mentioned in the COA based on the user’ s requirement.
    3. Individual organisms differ in their growth requirement and may show variable growth patterns on the medium
    prepared by the product.

    Performance and Evaluation


    Performance of the medium is expected when used as per the direction on the label within the expiry period when stored
    at recommended temperature

    Quality Control
    Appearance : Light yellow to brownish yellow homogeneous free flowing powder characteristic odour but not
    putrescent.
    Solubility : Soluble in distilled/ purified water, insoluble in alcohol and ether.
    Clarity : 1% w/v aqueous solution is clear without any haziness and free from visible foreign/black particles after
    autoclaving at 15 lbs pressure (121ºC) for 15 minutes.
    pH : pH of 2% w/v aqueous solution at 25ºC 6.0- 7.0
    Microbial Load :
    Bacterial Count : ≤ 2000 CFU/gram by plate method, when incubated at 30-35ºC for not less than 3 days
    Yeast & mould Count : ≤ 100 CFU/gram by plate method, when incubated at 20-25ºC for not less than 5 days.
    Test for pathogens : 1. Escherichia coli - Absent/gram of sample 2. Salmonella species- Absent/10 gram of sample
    3. Pseudomonas aeruginosa - Absent/gram of sample 4. Staphylococcus aureus - Absent/gram of sample 5. Candida
    albicans - Absent/gram of sample 6. Clostridia - Absent/gram of sample
    Indole Test : Tryptophan content: Passes

    . Page : 1 of 2
    Please refer disclaimer Overleaf.
    HiMedia Laboratories Technical Data

    Cultural Response : Cultural response observed after an incubation at 35-37ºC for 18-24 hours by preparing BHI
    Agar (M211) using BHI Powder as an ingredient.
    Cultural Response
    Organism Growth
    Candida albicans ATCC 26790 (WDCM 0054) Luxuriant
    Escherichia coli ATCC 25922 (WDCM 00013) Luxuriant
    Shigella flexneri ATCC 12022 (WDCM 00126) Luxuriant
    Staphylococcus aureus subsp. aureus ATCC Luxuriant
    25923 (WDCM 00034)
    Streptococcus pneumoniae ATCC 6303 Luxuriant

    Chemical Analysis :
    Total Nitrogen : ≥12.00 %
    Amino Nitrogen : ≥3.50 %
    Sodium chloride : ≤5.00 %
    Loss on drying : ≤5.00 %
    Residue on ignition : ≤12.00 %
    Storage and Shelf Life
    Store between 10 - 30°C in tightly closed container and away from bright light. Use before expiry date on label. On
    opening, product should be properly stored in dry ventilated area protected from extremes of temperature and sources
    Seal the container tightly after use.

    Disposal
    User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product.
    Follow established laboratory procedures in disposing of infectious materials and material that comes into contact with
    clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques.

    Storage temperature Do not use if package is damaged

    HiMedia Laboratories Pvt Limited


    C-40,21/Y, MIDC, Wagle Ind Area
    Thane(W)–400604,Maharashtra,India

    Revision : 07/2022
    Disclaimer :
    User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
    this and other related HiMedia™ publications.The information contained in this publication is based on our research and development
    work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
    specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
    for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified.Statements contained herein should
    not be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.

    .HiMedia Laboratories Pvt. Ltd. Reg.Office : Plot No:C-40, Road No: 21Y, MIDC,Wagle Industrial
    Page : 2 of 2
    Area,Thane(West)-400604,Maharashtra,INDIA.
    Tel:00-91-22-61471919/61169797/69034800,Fax:00-91-22-61471920.
    Email : techhelp@himedialabs.com Website : www.himedialabs.com

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