TDS K156

Technical Data
HiVibri-O-Trap Kit K156

Intended use :
Recommended for the selective enumeration and differentiation of Vibrio species from water samples.
Kit contains:
1. HiVibri-O-Trap Plate (RP001) - 10Nos.
2. Container for sample - 10Nos.
3. Syringe - 10Nos.
4. Disinfectant tablet - 10Nos.
5. Round sealing tape for Petri plate - 10Nos.
Directions:
1. Collect the water sample in the container provided
2. Open the pack. Remove one plate from the kit
3. With the help of provided syringe, inject 0.1 ml to 0.5ml of water sample into the
plate.
4. The sample will be evenly absorbed.
5. Incubate the plate at room temperature (28-30°C) for 24 hours.
6. Read the plate and count the colonies.
Salient features:
• Highly nutritious, supports the growth of desired microorganisms - Vibrio
• Chromogenic technology imparts distinct colours making identification easier
• Selective medium helps to inhibit the unwanted microbial flora.
• Enumeration and differentiation are easier.
• Disinfectant solution provided to ensure safe disposal.
Principle and Interpretation:
Vibrio species are gram negative bacteria. They are salt tolerant and are found in environments with higher salinity.
Vibrio has a major occurrence in sea food, which can lead to food poisoning. This gives Vibrio clinical significance.
Regular monitoring of samples for presence of Vibrio can prevent outbreaks and health hazards.
Detection of Vibrio species include enrichment in Alkaline Peptone water and further plating on selective media.
The most commonly used selective medium includes TCBS Agar based on sucrose fermentation with
bromothymol blue and thymol blue as indicator. Bile and high pH add to the selectivity of the medium. Other
most common medium used for enumeration and isolation include selective and differential medium like CPC
Agar base, Vibrio Agar, Proteose Agar, VP Medium, Vibrio Parahaemolyticus Sucrose Agar, Wagatsuma Agar
Base.
Chromogenic medium for Vibrio is more specific for Vibrio species wherein Vibrio cholerae gives purple colour,
Vibrio parahaemolyticus gives green colour and Vibrio fluvialis gives blue colour. The medium is selective and
inhibits the unwanted accompanying microflora.
Type of specimen:
Water samples
Warning and Precautions:
Read the label before opening the container. Wear protective gloves/protective clothing/eye protection/ face
protection. Follow good microbiological lab practices while handling specimens and culture. Standard precautions as
per established guidelines should be followed while handling specimens. Safety guidelines may be referred in
individual safety data sheets.
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when
stored at recommended temperature.
Quality Control:
Appearance:
90mm sealed Petri plate containing disc with medium
Cultural characteristics:
Cultural characteristics observed after injecting 0.1-0.5 ml of water sample and incubation at room temperature for
24-30 hours.
Organism Colour of colony
Vibrio cholerae purple
Vibrio parahaemolyticus green to blue green
Vibrio fluvialis blue
Vibrio furnissii light purple - purple (tiny colonies)
Vibrio alginolyticus light green
Vibrio proteolyticus colorless
Vibrio owensii light purple - purple (irregular flat colonies)
Vibrio plantisponcer violet blue
Staphylococcus aureus inhibited
Escherichia coli inhibited
Bacillus subtilis inhibited
Storage and Shelf Life:

Store between 2-8°C. Use before expiry date on the label. Product performance is best if used within stated expiry
period.
Disposal
On completion of work add a tablet of disinfectant into the container in which the sample was collected. Swirl it
for 5 seconds to mix. Add approximately 5ml into each plate. Swirl the plate for 5 seconds. Dispose off both the
container and the plate.
Revision: 02/2023
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and
other related HiMedia™ publications. The information contained in this publication is based on our research and development work and is to the best
of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications and information related
to the products at any time. Products are not intended for human or animal or therapeutic use but for laboratory,diagnostic, research or further
manufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressed or
implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. Corporate Office : Plot No.C-40, Road No.21Y, MIDC, Wagle Industrial Area, Thane (W) - 400604, India.
Customer care No.: 022-6147 1919 Email: techhelp@himedialabs.com Website: www.himedialabs.com

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Technical Data

HiVibri-O-Trap Kit K156

Performance and Evaluation

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