Technical Data: Enriched Thioglycollate Broth

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Technical Data
Enriched Thioglycollate Broth M738
Intended Use:
Recommended for isolation, cultivation and identification of a wide variety of obligate anaerobic bacteria from clinical and
non-clinical samples.
Composition**
Ingredients Gms / Litre
Tryptone 17.000
Soya peptone 3.000
Dextrose (Glucose) 6.000
Sodium chloride 2.500
Sodium thioglycollate 0.500
L-Cystine 0.250
Sodium sulphite 0.100
Hemin 0.005
Vitamin K1 0.0001
Agar 0.700
Sodium bicarbonate 1.000
Final pH ( at 25°C) 7.0±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Suspend 31.06 grams in 1000 ml purified/distilled water. Heat to boiling to dissolve the medium completely. Sterilize by
autoclaving at ∆118-121°C for 15 minutes. Aseptically add 10% rabbit or horse serum. Dispense in tubes or flasks as
desired. Cool and dry under 85% N₂,10% H₂ and 5% CO₂ atmosphere.
∆ corresponds to at 12-15 lbs pressure respectively.
Principle And Interpretation
Enriched Thioglycollate Medium is recommended for use in isolation and cultivation of fastidious and obligate anaerobic
bacteria from clinical materials (1). This medium is often used for susceptibility testing of anaerobes by broth disk elution
method. This medium is the modification of original Brewers formulation (2,3), with the addition of vitamin K1, sodium
bicarbonate, hemin and rabbit or horse serum.
Tryptone and soya peptone supports growth of wide variety of fastidious microorganisms. Sodium thioglycollate lowers the
oxidation-reduction potential for anaerobic growth and also neutralizes the bacteriostatic effect of mercurial compounds.
Most organisms show earlier and more vigorous growth in presence of dextrose, hemin and vitamin K1. Hemin is the
source of X-factor, which stimulates the growth of many microorganisms.
Type of specimen
Clinical : wound swabs, skin swabs or scrapings, tooth tartar etc.
Specimen Collection and Handling
For clinical samples follow appropriate techniques for handling specimens as per established guidelines
(4,5). After use, contaminated materials must be sterilized by autoclaving before discarding.
Warning and Precautions
In Vitro diagnostic use. For professional use only. Read the label before opening the container. Wear
protective gloves/protective clothing/eye protection/face protection. Follow good microbiological lab practices
while handling specimens and culture. Standard precautions as per established guidelines should be followed while
handling clinical specimens. Safety guidelines may be referred in individual safety data sheets.
Limitations
1. The tubes should not be reheated as frequent boiling leads to development of toxic products.
2. Prior to use the medium should be boiled once to remove the absorbed oxygen.
3. Before inoculation, the tubes should be brought to room temperature
4. The medium should not be used in fermentation process as medium contains yeast extract which is high in
carbohydrate content.
Please refer disclaimer Overleaf.

HiMedia Laboratories Technical Data
Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when
stored at recommended temperature.
Quality Control
Appearance
Cream to yellow homogeneous free flowing powder
Colour and Clarity of prepared medium
Light amber coloured, clear to slightly opalescent solution in tubes
Reaction
Reaction of 3.0% w/v aqueous solution at 25°C. pH : 7.0±0.2
pH
6.80-7.20
Cultural Response
Cultural characteristics observed under anaerobic condition, after an incubation at 35-37°C for 18-48 hours.
Organism Inoculum Growth
(CFU)
Bacteroides vulgatus 50-100 luxuriant
ATCC 8482
Clostridium perfringens 50-100 luxuriant
ATCC 12924
Clostridium sporogenes 50-100 luxuriant
ATCC 11437
Neisseria meningitidis 50-100 luxuriant
ATCC 13090
Streptococcus pyogenes 50-100 luxuriant
ATCC 19615
Storage and Shelf Life

Store between 10-30°C in tightly closed container and the prepared medium at 15-25°C. Use before expiry date on the
label. On opening, product should be properly stored dry, after tightly capping the bottle inorder to prevent lump
formation due to the hygroscopic nature of the product. Improper storage of the product may lead to lump formation. Store
in dry ventilated area protected from extremes of temperature and sources of ignition Seal the container tightly after use.
Product performance is best if used within stated expiry period. .
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product.
Follow established laboratory procedures in disposing of infectious materials and material that comes into contact
with clinical sample must be decontaminated and disposed of in accordance with current laboratory techniques (4,5).
Reference
1. Allen S. D., Siders J. A. and Movler M., 1985, In Manual of Clinical Microbiology, Lennette, Balows, Hausler
and Shadomy (Eds.), 4th Ed., ASM, Washington, D.C.
2. Brewer J. H., 1940 and 1943, J. Bacteriol., 39:10 and 46:39
3. Brewer J. H., 1943. J. Bacteriol., 46:39
4. Isenberg, H.D. Clinical Microbiology Procedures Handbook 2nd Editio
5. Jorgensen, J.H., Pfaller, M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
Manual of Clinical Microbiology, 11th Edition. Vol. 1.
Revision : 04/2022
Please refer disclaimer Overleaf.

HiMedia Laboratories Technical Data
HiMedia Laboratories Pvt. Limited, IVD In vitro diagnostic 30°C Storage temperature
Plot No.C-40, Road No.21Y, medical device
MIDC, Wagle Industrial Area,
Thane (W) -400604, MS, India 10°C
EC REP CEpartner4U, Esdoornlaan 13, Do not use if

CE Marking
3951DB Maarn, NL package is damaged
www.cepartner4u.eu
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in this and
other related HiMedia™ publications. The information contained in this publication is based on our research and development work and is to the best
of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to specifications and information related
to the products at any time. Products are not intended for human or animal or therapeutic use but for laboratory,diagnostic, research or further
manufacturing use only, unless otherwise specified. Statements contained herein should not be considered as a warranty of any kind, expressed or
implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. Corporate Office : Plot No.C-40, Road No.21Y, MIDC, Wagle Industrial Area, Thane (W) - 400604, India.
Customer care No.: 022-6147 1919 Email: techhelp@himedialabs.com Website: www.himedialabs.com

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Please refer disclaimer Overleaf.

Performance and Evaluation

Storage and Shelf Life

Please refer disclaimer Overleaf.

EC REP CEpartner4U, Esdoornlaan 13, Do not use if

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