LQ007A

Thioglycollate Broth LQ007A
Intended Use
Recommended for the recovery of anaerobic and facultative microorganisms. Sterile, in glass bottles.
Composition**
Ingredients Gms / Litre
Tryptone 15.000
Yeast extract 5.000
Dextrose (Glucose) 5.500
Sodium chloride 2.500
L-Cystine 0.500
Sodium thioglycollate 0.500
Final pH ( at 25°C) 7.1±0.2
**Formula adjusted, standardized to suit performance parameters
Directions
Label the ready to use blood culture bottle. Remove the Aluminium foil cap. Disinfect the part of the rubber stopper which is
now exposed. Draw patient's blood with the sterile or disposable needle and syringe as explained in specimen collection and
disposable column. Transfer the blood sample immediately into the culture bottle by puncturing the rubber stopper with the
needle and injecting the blood. Venting: Use sterile venting needle (LA038). Keep the bottle in an upright position preferably
in a biological safety cabinet, place an alcohol swab over the rubber stopper and insert the venting needle with filter through
it. Insertion and withdrawal of the needle should be done in a straight line. discard the needle and mix the contents by gently
inverting the bottle 2-3 times. Do Not vent the bottle for anaerobic cultures. Incubate at 35-37°C for 24-72 hours and further
for seven days.
Principle And Interpretation

Alternative Thioglycollate Medium is formulated as described in the N.I.H. memorandum (5). It is used for the sterility
testing of certain biological products which are turbid or viscous. Both the media have similar composition, except agar and
resazurin that are not included in Alternative Thioglycollate Medium. This deletion makes it suitable for sterility testing of
viscous products. Tryptone serves as a source of nitrogen and carbon compounds, long chain amino acids and other essential
nutrients. Yeast extract serve as source of essential nutrients to the contaminants, if present. Dextrose serves as the energy
source. Sodium chloride maintains the osmotic equilibrium of the medium whereas L-cystine, an amino acid, also serves as
source of essential growth factors. Sodium thioglycollate and L-cystine lower the oxidation-reduction potential of the
medium by removing oxygen to maintain a low Eh. Sodium thioglycollate also helps to neutralize the toxic effects of
mercurial preservatives (6,7).
Type of specimen
Clinical : wound swabs, skin swabs or scrapings, tooth tartar etc. Pharmaceutical: Sterility testing of viscous products.
Specimen Collection and Handling

For clinical samples follow appropriate techniques for handling specimens as per established guidelines (1,2).
For pharmaceutical products, follow appropriate techniques for sample processing in case of viscous materials as mentioned
under sterility. (8)
Warning and Precautions

In Vitro diagnostic use only. Read the label before opening the container. Wear protective gloves/protective clothing/
eye protection/face protection. Follow good microbiological lab practices while handling specimens and culture. Standard
precautions as per established guidelines should be followed while handling clinical specimens. Safety guidelines may be
referred in individual safety data sheets.
Please refer disclaimer Overleaf.
HiMedia Laboratories Technical Data
Limitations
1. Proper anaerobic conditions must be maintained for optimal recovery of organisms
Performance and Evaluation

Performance of the medium is expected when used as per the direction on the label within the expiry period when stored
at recommended temperature.
Quality Control
Appearance
Sterile clear Thioglycollate Broth in glass bottle
Colour
Light yellow coloured clear solution.
Quantity of Medium
70ml of medium in glass bottle. (For Adult Use)
Reaction
6.90-7.30
Sterility test
Passes release criteria
Cultural response
Cultural characteristics was observed after incubation at 35-37°C for 24-72 hours
Organism Inoculum Growth under Growth under
(CFU) anaerobic conditions aerobic conditions
*Clostridium sporogenes 50 -100 luxuriant -

ATCC 19404 (00008*)
ATCC 11437
NBRC 14293
*Clostridium perfringens 50 -100 luxuriant -
ATCC 13124 (00007*)
Staphylococcus aureus 50 -100 - luxuriant
subsp. aureus ATCC
25923 (00034*)
Staphylococcus aureus 50 -100 - luxuriant
subsp. aureus ATCC 6538
(00032*)
Pseudomonas aeruginosa 50 -100 - luxuriant
ATCC 27853 (00025*)
Pseudomonas aeruginosa 50 -100 - luxuriant
ATCC 9027 (00026*)
Escherichia coli ATCC 50 -100 - luxuriant
25922 (00013*)
Escherichia coli ATCC 8739 50 -100 -
luxuriant
(00012*)
Escherichia coli NCTC 9002 50 -100 - luxuriant
Salmonella Abony NCTC 50 -100 - luxuriant
6017 (00029*)
Salmonella Typhimurium 50 -100 - luxuriant
ATCC 14028 (00031*)
*Bacteroides fragilis ATCC 50 -100 luxuriant -
23745
* Bacteroides vulgatus ATCC 50 -100 luxuriant -
8482
Key : *Corresponding WDCM numbers.
Please refer disclaimer Overleaf.

HiMedia Laboratories Technical Data
Storage and Shelf Life

Store between 2-8°C. Use before expiry date on the label.
Product performance is best if used within stated expiry period.
Disposal
User must ensure safe disposal by autoclaving and/or incineration of used or unusable preparations of this product. Follow
established laboratory procedures in disposing of infectious materials and material that comes into contact with clinical
sample must be decontaminated and disposed of in accordance with current laboratory techniques (1,2).
Reference
1.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.

2. Jorgensen,J.H., Pfaller , M.A., Carroll, K.C., Funke, G., Landry, M.L., Richter, S.S and Warnock., D.W. (2015)
Manual of Clinical Microbiology, 11th Edition. Vol. 1.
3. Lapage S., Shelton J. and Mitchell T., 1970, Methods in Microbiology', Norris J. and Ribbons D., (Eds.), Vol. 3A, Academic
Press, London.
4. MacFaddin J. F., 2000, Biochemical Tests for Identification of Medical Bacteria, 3rd Ed., Lippincott, Williams and Wilkins,
Baltimore.
5. N.I.H. Memorandum, 1955: Culture Media for Sterility Tests, 4th Revision.
6. Nungester, Hood and Warren, 1943, Proc. Soc. Exp. Biol. Med., 52: 287
7. Portwood, 1944, J. Bacteriol., 48: 255
8.The United States Pharmacopoeia, 2019, The United States Pharmacopoeial Convention.Rockville, MD.
Revision : 00 / 2019
In vitro diagnostic medical

IVD device
CE Marking
25°C Storage temperature
15°C
Do not use if package is

damaged
HiMedia Laboratories Pvt. Limited,

23 Vadhani Industrial Estate,
LBS Marg,Mumbai-86,MS,India
CE Partner 4U ,Esdoornlaan 13, 3951

EC REP
DB Maarn The Netherlands,
www.cepartner 4u.eu
Disclaimer :
User must ensure suitability of the product(s) in their application prior to use. Products conform solely to the information contained in
this and other related HiMedia™ publications. The information contained in this publication is based on our research and development
work and is to the best of our knowledge true and accurate. HiMedia™ Laboratories Pvt Ltd reserves the right to make changes to
specifications and information related to the products at any time. Products are not intended for human or animal or therapeutic use but
for laboratory,diagnostic, research or further manufacturing use only, unless otherwise specified. Statements contained herein should not
be considered as a warranty of any kind, expressed or implied, and no liability is accepted for infringement of any patents.
HiMedia Laboratories Pvt. Ltd. Reg.office : 23, Vadhani Ind.Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6116 9797 Corporate office :
A-516,Swastik Disha Business Park,Via Vadhani Ind. Est., LBS Marg, Mumbai-400086, India. Customer care No.: 022-6147 1919 Email:
techhelp@himedialabs.com Website: www.himedialabs.com

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Thioglycollate Broth LQ007A

Principle And Interpretation

Specimen Collection and Handling

Warning and Precautions

Performance and Evaluation

*Clostridium sporogenes 50 -100 luxuriant -

Please refer disclaimer Overleaf.

Storage and Shelf Life

1.Isenberg, H.D. Clinical Microbiology Procedures Handbook. 2nd Edition.

In vitro diagnostic medical

25°C Storage temperature

Do not use if package is

HiMedia Laboratories Pvt. Limited,

CE Partner 4U ,Esdoornlaan 13, 3951

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