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    Che 170 Homework #2 (Due Thursday, October 8)

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    Timothy Hill
    This document contains the homework assignment for a chemical engineering class. It includes 5 problems related to biological chemicals and cell growth modeling. Problem 1 involves drawing chemical structures, explaining how ATP is used for energy storage, calculating ATP turnover rate, and discussing potential issues with cell-based production of adenosine. Problem 2 asks to draw diagrams comparing E. coli and yeast cell structures. Problems 3-5 involve calculating cell growth kinetic parameters like growth rate and yield from batch culture experimental data.

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    Che 170 Homework #2 (Due Thursday, October 8)

    Uploaded by

    Timothy Hill
    70 views3 pages
    This document contains the homework assignment for a chemical engineering class. It includes 5 problems related to biological chemicals and cell growth modeling. Problem 1 involves drawing chemical structures, explaining how ATP is used for energy storage, calculating ATP turnover rate, and discussing potential issues with cell-based production of adenosine. Problem 2 asks to draw diagrams comparing E. coli and yeast cell structures. Problems 3-5 involve calculating cell growth kinetic parameters like growth rate and yield from batch culture experimental data.

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    This document contains the homework assignment for a chemical engineering class. It includes 5 problems related to biological chemicals and cell growth modeling. Problem 1 involves drawing chemical structures, explaining how ATP is used for energy storage, calculating ATP turnover rate, and discussing potential issues with cell-based production of adenosine. Problem 2 asks to draw diagrams comparing E. coli and yeast cell structures. Problems 3-5 involve calculating cell growth kinetic parameters like growth rate and yield from batch culture experimental data.

    Copyright:

    © All Rights Reserved
    Download as PDF, TXT or read online from Scribd
    Download as pdf or txt
    70 views3 pages

    Che 170 Homework #2 (Due Thursday, October 8)

    Uploaded by

    Timothy Hill
    This document contains the homework assignment for a chemical engineering class. It includes 5 problems related to biological chemicals and cell growth modeling. Problem 1 involves drawing chemical structures, explaining how ATP is used for energy storage, calculating ATP turnover rate, and discussing potential issues with cell-based production of adenosine. Problem 2 asks to draw diagrams comparing E. coli and yeast cell structures. Problems 3-5 involve calculating cell growth kinetic parameters like growth rate and yield from batch culture experimental data.

    Copyright:

    © All Rights Reserved
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    of 3

    ChE

    170 Homework #2 (Due Thursday, October 8)


    1. Biological Chemicals (30 Points): Adenosine is a building block for many important
    biomolecules that are involved in cellular energy, cell signaling, and cellular regulation.

    (a) Draw the structure of adenosine triphosphate (ATP) and cyclic adenosine
    monophosphate (cAMP).

    (b) ATP is an important energy currency in cells. With reference to your structures above,
    explain why ATP is used by cells to store & release energy, while another adenosine-
    containing molecule like cAMP is not. (hint: your answer should include a discussion of
    G)


    (c) A 70 kg adult (~150 lbs) could meet his or her entire energy needs for one day by eating
    3 moles of glucose (540 g). Assume that each molecule of glucose generates 30 ATP when it
    is oxidized. The concentration of ATP is maintained in cells at about 2 mM, and a 70 kg
    adult has about 25 L of intracellular fluid. Given that the ATP concentration remains
    constant in cells, calculate how many times per day (on average) each ATP molecule in the
    body is hydrolyzed and resynthesized.


    (d) Suppose you are asked to formulate a cell-based bioprocessing strategy to produce
    adenosine. What cellular platform would you select for production (without any additional
    information)


    (e) Given what you know about adenosine, list one potential problem you see with cell-
    based production of this product.



    2. Comparing Cellular Platforms (20 Points): In class, we talked about the differences
    between two key model microbes used in bioprocessing. Below, draw an engineers view
    of the cells and parts of:
    (a.) E. coli



    (b.) S. cerevisiae




    3. Monitoring Cell Growth (10 points): Anaerobic gut fungi take up residence within the
    digestive tract of large herbivores, where they have evolved to break down plant biomass
    through the secretion of powerful enzymes. In class, you had the chance to observe these
    microbial cells close up, where you (hopefully) observed that they produce a very
    different looking biomass compared to yeast and E. coli. If not, please see a picture of them
    on this site: http://omalleylab.weebly.com/research.html.

    Suppose that you have isolated a strain of gut fungi and you are proliferating the fungi in
    the lab. You plan to carry out a batch growth experiment to determine doubling time of
    these fungi.

    (a.) What is one direct method you can think of to measure cell growth of these cells?
    Describe any disadvantages associated with your chosen method.

    (b.) What is one indirect method that you could use to measure cell growth? Please explain
    your answer.


    4. Batch Cell Growth (15 points): A strain of mold was grown in a batch culture on glucose
    and the following data were obtained:
    Time (hr) Cell Concentration (g/L) Glucose Concentration (g/L)
    0
    1.25
    100
    9
    2.45
    97
    16
    5.1
    90.4
    23
    10.5
    76.9
    30
    22
    48.1
    34
    33
    20.6
    36
    37.5
    9.38
    40
    41
    0.63

    For this system:
    (a.) Calculate the maximum net specific growth rate, max
    (b.) Calculate the growth yield, YX/S
    (c.) What is the maximum cell concentration you would expect if 150 g of glucose were
    used with the same size inoculum?








    5. Batch Cell Growth (25 points): A simple, batch fermentation of E. coli growing on an
    alcohol gave the results shown in the below table:

    Time (hour)
    X (g/L)
    S (g/L)
    0
    .2
    9.23
    2
    .211
    9.21
    4
    .305
    9.07
    8
    .98
    8.03
    10
    1.77
    6.8
    12
    3.2
    4.6
    14
    5.6
    0.92
    16
    6.15
    0.077
    18
    6.2
    0

    Calculate:
    (a.) Maximum growth rate (m)
    (b.) Yield on substrate (YX/S)
    (c.) Mass doubling time (d)
    (d.) Saturation constant (KS)
    (e.) Specific growth rate (net) at t = 10 hours

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