INSTRUCTIONS

CX21
EDUCATION MICROSCOPE
This instruction
manual is for the
Olympus Education
Microscopes Model
CX21. To ensure the
safety, obtain optimum
performance and to
familiarize yourself
fully with the use of
this microscope, we
recommend that you
study this manual
thoroughly before
operating the
microscope. Retain
this instruction manual
in an easily accessible
place near the work
desk for future
reference.

IVD

AX7251

CX21

CONTENTS

IMPORTANT Be sure to read this section for safe use of the equipment.

1-3

1
STANDARD COMPONENT UNITS
4

2
NOMENCLATURE
5

SUMMARY OF BRIGHTFIELD OBSERVATION PROCEDURE

DETAILED OBSERVATION PROCEDURE

7-11

1
Turning the Lamp ON ...........................................

7
2 Placing Specimen on the Stage
.................... 7

3
Adjusting the Focus ................................................

8
4 Adjusting the Interpupillary Distance.....................

9
5
Adjusting the Diopter ............................................

6
Adjusting the Condenser Position and Aperture Iris Diaphragm .............................................................

10
7
Switching the Objectives ............................

10
8 Using the 100X Immersion Objective.....................

11

ONE-POINT ADVICE

TROUBLESHOOTING GUIDE

SPECI
FICAT
IONS

OPTICAL CHARACTERISTICS

15

ASSEMBLY
16

10 OPTIONAL ACCESSORIES

12

17-18

13-14
19-21

10-1
System Diagram of Optional Accessories ..............................................................................
19

10-2
Installation and Operation of Optional Accessories .......................................
19-21

1
Cord Hanger CH3-CH ............................................................................................................................................................................................

19

2
Filter Holder CH2-FH ...............................................................................................................................................................................................

20

3
Darkfield Ring CH2-DS ........................................................................................................................................................................................

20

4
Reflection Mirror CH20-MM ..........................................................................................................................................................................

20

5
Eyepieces WHC15X/WHC15X-H.......................................................................................................................................................................................................

21

6
Using the eyepiece micrometer ........................................................................................................................................................................................................

21

PROPER SELECTION OF THE POWER SUPPLY CORD

22-23

.......................................................

IMPORTANT

SAFETY PRECAUTIONS

1.
After the equipment has been used in an observation of a specimen

that is accompanied with a potential of infection, clean the parts

coming in contact with the specimen to prevent infection.

Moving this product is accompanied with the risk of dropping the

specimen. Be sure to remove the specimen before moving this prod-

uct.

In case the specimen is damaged by erroneous operation, promptly

take the infection prevention measures.

2.
To avoid potential shock hazards and burns when replacing the lamp

@
bulb, set the main switch 1 to (OFF) then disconnect the power cord

from the wall outlet in advance. Whenever you replace the bulb during

Fig. 1
use or right after use, allow the lamp socket 2 and bulb to cool before

touching. (Fig. 1)

Applicable lamp bulb: 6V20WHAL halogen bulb (Philips Type 7388)

3.
Install the microscope on a sturdy, level table or bench so as not to block

the air vents on the underside of the base.

Do not place the microscope on a flexible surface, as this could result in

blocking the air vents and cause overheating or a fire.

4.
Always use the power cord provided by Olympus. If no power cord is

provided, please select the proper power cord by referring to the section

PROPER SELECTION OF THE POWER SUPPLY CORD at the end of

this instruction manual. If the proper power cord is not used, product

safety performance cannot be warranted.

5.
When installing the microscope, route the power cord away from the

microscope frame. Should the power cord come in contact with a hot

part, the power cord could melt and cause electric shock.

6.
Always ensure that the grounding terminal of the microscope and that

of the wall outlet are properly connected. If the equipment is not grounded,

Olympus can no longer warrant the electrical safety performance of the

equipment.

7.
Never allow metallic objects penetrate into the air vents of the micro-

scope frame as this could result in electrical shock, personal injury and

equipment damage.

8.
After operation or in case of abnormality, be sure to disconnect the power

cord from the connector on the microscope or from the wall power outlet.

Safety Symbols

The following symbols are found on the microscope. Study the meaning of the symbols and always use the
equipment in the safest possible manner.

Symbol
Explanation

Indicates that the surface becomes hot, and should not be touched with bare hands.

Before use, carefully read the instruction manual. Improper use could result in personal injury to the user and/or
damage to the equipment.

Indicates that the main switch is ON.

Indicates that the main switch is OFF.

CX21

Warning Label

A warning indication label is attached to every part where special precaution is required when handling and using the
microscope. Always heed the warnings.

Warning label
Bottom of microscope
[Warning against high temperature in

position
frame
lamp bulb replacement]

If the warning label is stained or peeled off, contact Olympus.

Getting Ready

1.
A microscope is a precision instrument. Handle it with care and avoid

subjecting it to sudden or severe impact.

2.
Do not use the microscope where it is subjected to direct sunlight,

high temperature and humidity, dust or vibrations. (For the operating

conditions, see chapter 7, SPECIFICATIONS on Page 15.)

@
3.
Always use the tension adjustment ring to adjust the rotation tension of

the coarse adjustment knob.

4.
The microscope is ventilated by natural convection. Be sure to leave

enough spaces (10 cm or more) around it when installing it.

5.
When carrying the microscope, hold both sides around the hole of the

arm as shown in Fig. 2 and carry carefully.

Fig. 2

#To prevent damage, do not hold the microscope by the stage 1 or observation tube 2.

Be sure to remove the specimen; otherwise, it may fall.

Maintenance and Storage

Clean all glass components by wiping gently with gauze. To remove fingerprints or oil smudges, wipe with gauze slightly
moistened with a mixture of ether (70%) and alcohol (30%).

Since solvents such as ether and alcohol are highly flammable, they must be handled carefully. Be sure to keep these
chemicals away from open flames or potential sources of electrical sparks for example, electrical equip-ment that is
being switched on or off. Also remember to always use these chemicals only in a well-ventilated room.

Do not attempt to use organic solvents to clean the microscope components other than the glass components. To clean
them, use a lint-free, soft cloth slightly moistened with a diluted neutral detergent.
Do not disassemble any part of the microscope as this could result in malfunction or reduced performance.

When not using the microscope, ensure that the frame is cooled down and store it in a dry locker or cover it with a dust
cover.

To clean the condenser, fully loosen the securing knob 1, then remove the condenser by lowering it with the condenser
height adjustment knob 2, and wipe the front lens of the condenser.
The condenser can be attached by reversing the above removal

procedure.

@ 6. When disposing of the microscope. Check the regulations and rules

of your local government and be sure to observe them.

Fig. 3

Caution

If the
microscope is
used in a
manner not
specified by
this manual, the
safety of the
user may be
imperiled. In
addition, the
equipment may
also be
damaged.
Always use the
equipment as
outlined in this
instruction
manual.

The following
symbols are
used to set off
text in this
instruction
manual.

: Indicates
that failure to
follow the
instructions in
the warning
could result in
bodily harm to
the user and/or
damage to
equipment
(including
objects in the
vicinity of the
equipment).

# : Indicates
that failure to
follow the

instructions
could result in
damage to
equipment. } :
Indicates
commentary
(for ease of
operation and
maintenance).

This device
complies with
the
requirements of
directive
98/79/EC
concerning in
vitro diagnostic
medical
devices. CE
marking means
the conformity
to the directive.

NOTE: This
equipment has
been tested
and found to
comply with the
limits for a
Class A digital
device,

pursuant to
Part 15 of the
FCC Rules.
These limits are
designed to
provide
reasonable
protection

against harmful
interference
when the
equipment is
operated in a
commercial
environment.
This

equipment
generates, uses,
and can radiate
radio frequency
energy and, if
not installed and
used in

accordance with
the instruction
manual, may
cause harmful
interference to
radio
communications
.

Operation of this
equipment in a
residential area
is likely to cause
harmful
interference in
which case

the user will be

required to
correct the
interference at
his own
expense.

FCC WARNING:
Changes or
modifications
not expressly
approved by the
party
responsible for
compliance

could void the

users authority
to operate the
equipment.

CX21

STANDARDCOMPONENTUNITS

}After opening the package, make sure that the correct component units for the selected set are present.

The differences between the CX21FS1 and CX21FS2 lie in the number of objectives and presence of immersion oil.

#The objectives have been screwed in tightly to prevent them from being loosened during transportation. To remove an objective, turn
it counterclockwise while holding it with a rubber sheet, etc., so that your fingers dont slip.

Binocular observation
Eyepieces
tube

(Fixed at 10X)

Revolving nosepiece

Objectives

4X, 10X, 40X, 100X (CX21FS1)

4X, 10X, 40X (CX21FS2)

Microscope frame
Stage

Condenser

Daylight (blue) filter

6V20WHAL halogen bulb

Immersion oil

 Provided with a set including

the 100X objective.

Optional Accessories

Reflection Mirror

 Cord Hanger
Filter Holder
Darkfield Ring

CH3-CH
CH2-FH
CH2-DS

CH20-MM

Eyepieces

WHC15X

WHC15X-H

NOMENCLATURE

}The following items have been attached at the factory to prevent deterioration during transport. Remove these
items and retain them for future use. Then loosen the observation tube clamping knob and correct the orientation
of the eyepieces as shown in the illustration below.

1 Revolving nosepiece/observation tube transport band

2 Stage and specimen holder protection sheet

3 Protective pad below the stage

}Attach the lamp bulb and power cord as described in chapter 9, ASSEMBLY on pages 17-18.

Revolving nosepiece (Page 10)

Specimen holder (Page 7)

Aperture iris diaphragm ring (Page 10)

Filter holder (Page 20)

Diopter adjustment ring

(Page 9)

Coarse adjustment knob (Page 8)

Observation tube
clamping knob
Fine adjustment knob (Page 8)

Pre-focusing knob (Page

9)
Specimen holder Y-axis feed knob (Page 7)

Specimen holder X-axis feed knob (Page 7)

Main switch (Page 7)

: Power ON

: Power OFF

Light intensity adjustment knob (Page 7)

}For detailed description of each control, refer to the page indicated inside parentheses.

CX21
SUMMARYOFBRIGHTFIELDOBSERVATIONPROCEDURE

(Controls Used)
(Page)

Set the main switch to I " (ON) and adjust

1Main switch
(P. 7)

the brightness.
2Light intensity adjustment knob
(P. 7)

3Specimen holder
(P. 7)

Place the specimen on the stage.

4X-axis/Y-axis feed knobs

(P. 7)

5Revolving nosepiece
(P. 10)

Engage the 10X objective in the light path.

Bring the specimen in focus

6Coarse/fine focus adjustment knobs
(P. 8)

Adjust the interpupillary distance.

7Binocular tube
(P. 9)

Adjust the diopter.

8Diopter adjustment ring
(P. 9)

9Aperture iris diaphragm ring

(P. 10)

Adjust the aperture iris diaphragm.

Engage the objective to be used in the light

5Revolving nosepiece
(P. 10)

path and bring the specimen in focus.

6Coarse/fine adjustment knobs

(P. 8)

aFilters (Diameter: 32.5 mm or 45 mm)

(P. 20)

Engage the required filters.

Adjust the brightness.

2Light intensity adjustment knob
(P. 7)

Start observation.

87

5
6

a
4

DETAILEDOBSERVATIONPROCEDURE
(Fig. 4)

Turning the Lamp

ON

Set the main switch 1 to I (ON).

Rotating the light intensity adjustment knob 2 in the

direction of the arrow increases brightness and
rotating it in the opposite direction de-creases
brightness. The figures around the knob indicate the
reference voltage values.

Fig. 4

2 Placing Specimen on the Stage

(Fig. 5)

@
#Place the specimen gently. If the bow-shaped lever is returned with

a strong force or the knob 1 of the bow-shaped lever is released in

the middle, the slide glass may be broken.

1.
Rotate the coarse adjustment knob 2 in the direction of the arrow to fully

lower the stage.

2.
Open the bow-shaped lever 3 outward, place the specimen by sliding

the specimen glass plates on the stage from the front toward the rear.

|
3.
After sliding the specimen glass plates all the way, return the bow-shaped

lever 3 gently.

4. Rotating the upper knob which is the Y-axis feed knob 4 moves the

Fig. 5
specimen in the vertical direction. Rotating the lower knob which is the X-

axis feed knob 5 moves it in the horizontal direction.

#Do not move the specimen holder directly by hand, for this will

damage the rotary mechanisms of the above knobs.

#When the specimen holder reaches the stopper position, the

rotation force of the above knobs become heavy. Stop rotating

the knob at this time.

Cover glass

Cover glass

Fig. 6

This is the glass plate placed on the specimen. To allow

the objective manifest the full performance, the cover
glass thickness, which is the distance from its surface to
the specimen surface, should ideally be 0.17 mm.

Slide glass

Placing Specimen

Slide glass

This glass plate

should ideally have

a length of 76 mm, width of 26 mm and thickness

between 0.9 and 1.4 mm.

CX21

Specimen holder scales (Fig. 7)

}Theses scales allow the position (coordinates) being observed on the

specimen to be identified. Even after the specimen is moved, it can be

returned easily to the original position.

1.
The horizontal coordinate can be read at position 1 on the specimen

holder.

2.
The vertical coordinate can be read at the position of index line 2.

Fig. 7

Adjusting the Focus

Focusing Procedure

1. Rotate the coarse adjustment knob @ in the direction of the

arrow so that the objective 3 is as close as possible to the
specimen.

Fig. 8

2. While observing the specimen through the eyepieces, slowly rotate

the @ coarse adjustment knob 1 in the opposite direction to the arrow
to lower

the stage.

3. When coarse focusing of the specimen is obtained, rotate the

fine

adjustment knob 2 to adjust to precise focus.

Working Distance (WD)

}The WD refers to the distance between each objective and the

specimen, when precise focus of the specimen is obtained.

Objective
4X
10X

40X
100X

Magnification

Fig. 9

(Fig. 8)

Adjusting the Tension of the Coarse Adjustment Knob (Fig. 9)

@
The tension of the coarse focus adjustment knob has been designed

WD (mm)

adjustable with a ring. Insert the tip of a large flat-blade

screwdriver into the groove 2 on the tension adjustment ring 1 and
rotate the ring. Rotating it clockwise (in the direction of the arrow)
increases the tension and counterclockwise decreases the tension.

18.5
10.6
0.6
0.13

If the stage descends on its own or if the specimen gets out of focus
quickly even when it is brought into focus using the fine adjustment
knob, it means that the tension of the coarse adjustment knob is too
low. Turn the ring 1 in the direction of the arrow to increase the tension.

Pre-focusing Knob (Fig. 10)

}The pre-focusing knob controls the mechanism for preventing collision between the specimen and objective.

1. After bringing the specimen into focus, turn the pre-focusing knob 1 inside the hole of the arm so that the prefocusing mechanism hits the stage guide.

To provide a certain margin for focusing, rotate the knob by about half turn backward from the stopped position.
#If the function of this mechanism is not required, set the pre-focusing

knob 1 at the highest position.

Fig. 10

4 Adjusting the Interpupillary Distance

(Fig. 11)

}The interpupillary distance adjustment consists of regulating the two eye-pieces according to that between your eyes
so that you can observe a single microscopic image through two eyepieces. This greatly helps to reduce fatigue during
observation.

While looking through the eyepieces, move both eyepieces until the left and right fields of view coincide completely.
The position of index dot indicates the interpupillary distance value. }Note your interpupillary distance so that it can be quickly
duplicated.

Fig. 11

5 Adjusting the Diopter

(Fig. 12)

}The diopter adjustment consists of compensating for the difference in eyesight between your eyes.
1. While looking through the right eyepiece with your right eye, rotate the coarse and fine focus adjustment knobs to
bring the specimen into focus.

While looking through the left eyepiece with your left eye, rotate only the diopter adjustment ring 1 to focus on the
specimen.

Fig. 12

Using the Eye Shades (Fig. 13)

When Wearing Eyeglasses

Use with the eye shades in the normal, folded-down position. This will prevent the eyeglasses from being scratched.

When Not Wearing Eyeglasses

Extend the folded eye shades in the direction of the arrow to prevent extraneous light from entering between the eyepieces
and eyes.

Fig. 13

CX21

6 Adjusting the Condenser Position and Aperture Iris Diaphragm

(Fig. 14)

}The condenser is usually used in the highest position. If the entire observed field of view is not bright enough, brightness
may be improved by lowering the condenser slightly.

1. Rotate the condenser height adjustment knob 1 to move the condenser to the highest position.
2. The aperture iris diaphragm ring 2 has an objective magnification scale (4X, 10X, 40X, 100X). Rotate the ring so that the
magnification of the objective in use faces frontward.

Fig. 14

7 Switching the Objectives

(Fig. 15)

Hold and rotate the revolving nosepiece 1 so that the objective to be

used come exactly above the specimen.

Fig. 15

10

8
Using the 100X Immersion Objective
(Fig. 16)

}The designated immersion oil should be attached to the top lens of the

100X immersion objective. Otherwise, the observed image will be unable

to be focused on.

#Always use immersion oil supplied by Olympus.

1.
Focus on the specimen using all objectives, starting from the lowest-

power objective to higher-power objective.

2.
Before engaging the immersion objective in the light path, place a drop

of provided immersion oil onto the specimen at the area to be observed.

3. Rotate the revolving nosepiece to engage the immersion objective and rotate the fine adjustment knob to bring the
specimen into focus.
#Since air bubbles in the oil will affect the image quality, make sure

Fig. 16

that the oil is free of bubbles.

To remove bubbles, rotate the revolving nosepiece slightly to move the oil-immersed objective by one reciprocation or
two.
}The condenser of this microscope manifests the full performance when oil is placed between the slide glass and the front lens of
condenser. If oil is not attached there, the observation image may become slightly dark.

After use, remove oil from the objective front lens by wiping with gauze slightly moistened with an ether (70%)/alcohol (30%)
mixture.

Caution in use of immersion oil

If immersion oil enters your eyes or contacts your skin, immediately take the following treatment.
Eyes: Rinse with fresh water (for 15 minutes or more). Skin : Rise with water and soap.
If the appearance of the eyes or skin is altered or pain persists, immediately see your doctor.

11

Approx. 0.9 mm, about half of actual field of view.

CX21

ONEPOINTADVICE

How To Track a Microscopic Image

To track an image

moving leftwards

Move the slide

(or to move the

glass to the

specimen image

left.

rightwards):

Direction of moving the image

To move the
Move the slide

specimen image
glass down.

upward:

}The image observed through the microscope moves in directions opposite to the actual up-down and left-right
movements of the specimen.

Total Magnification

The size of the specimen image for observation is obtained by multiplying the eyepiece magnification by the
objective magnification. This value is referred to as total magnification.

Example: Eyepiece (10X) x Objective (40X) = 400X

Resolution

Resolution determines how finely a lens is able to distinguish the details of a specimen.

The resolution of a microscope is mainly determined by the ability of the objective and is scarcely related to that of the
eyepieces. The only function of the eyepieces is to magnify an image already resolved by the objective.

Although both configurations above provide the same total magnification, the higher magnification of the objective
yields a better specimen resolution.

Field Number (FN)

The field number is the diameter of the image observed through an eyepiece, represented in millimeters.

10X eyepiece: 18 mm WHC15X: 12 mm

Actual Field of View

When the 10X

eyepiece above
and 10X objective
are used, the
actual field of view
is equal to:

The actual field of view is the size on the specimen actually observed in the
microscope. This yields approximate size of the actual specimen.
Actual field of view
= 18/10 = 1.8 mm
Actual field of view = Field number/Objective magnification

Actual field of view:

1.8 mm

12

TROUBLESHOOTINGGUIDE

Under certain conditions, performance of the unit may be adversely affected by factors other than defects. If problems occur,
please review the following list and take remedial action as needed. If you cannot solve the problem after checking the entire list,
please contact Olympus for assistance.

Problem
Cause
Remedy
Page

1. Uneven brightness in observation

The objective is not engaged in the light
Engage the objective into position until
10

field.
path.
it clicks.

The condenser is too low.

Raise it to the upper limit.
10

The objective, eyepiece, condenser and/

Clean them thoroughly.
2

or window lens are dirty.

2. Dust or stains are visible in obserThe eyepiece, condenser, window lens

Clean them thoroughly.

2

vation field.
and/or specimen glasses are dirty.

3. Observation image glares.

The condenser is too low.
Raise it.
10

The condenser iris diaphragm ring is

Adjust the aperture according to the ob10

stopped down excessively.

jective magnification.

4. Observation image is whitishThe objective is not engaged in the light

Engage the objective into position until
10

blurred or unclear.
path.
it clicks.

The objective, eyepiece, condenser and/

Clean them thoroughly.
2

or specimen glasses are dirty.

Immersion oil is not used with an immerUse immersion oil.

11

sion objective.

Bubbles are mixed in the immersion oil.

Remove the bubbles.
11

The specified immersion oil is not used.

Use the immersion oil supplied by
11

Olympus.

5. Part of image is defocused or image

The objective is not properly engaged in
Engage the objective into position until
10

looks like its flowing.

the light path.
it clicks.

The specimen is not set properly on the

Set the specimen correctly on the stage
7

stage.
and secure using the specimen holder.

6. High-magnification objective
The specimen is upside down.
Set the specimen correctly with the

touches specimen just before

cover glass on the top.

7

coming into focus.

7. The tension of coarse adjustment

The coarse adjustment knob tension adLoosen the ring to adjust to proper
8

knob it too high.

justment ring is set too tight.
tension.

8. Focusing is impossible (because

The pre-focusing knob is positioned too
Raise its position.
9

the stage cannot be raised).

low.

9. The stage lowers by its own weight

The coarse adjustment knob tension
Tighten the ring to adjust to proper ten-

or focusing is lost due to slippage

adjustment ring is set too loose.
sion.
8

of the knob.

10. Coarse focus adjustment cannot

The condenser is too low.
Raise it.
10

lower the stage low enough.

11. Fields of view of two eyes do not

The interpupillary distance is not adjusted
Adjust it properly.
9

match.
properly.

Diopter compensation for the two eyes is

Adjust it correctly.
9

not set.

The left and right eyepieces are different.

Replace one of them so that the left
--

and right eyepieces are identical.

13

CX21

Problem
Cause
Remedy
Page

12. Objective hits the specimen when

The specimen is upside down.
Set the specimen correctly with the
7

an objective is switched to a higher-

cover glass on the top.

magnification objective.

The cover glass is too thick.

Use a cover glass with thickness of
7

0.17 mm.

13. Lamp bulb does not light.

Lamp bulb is not mounted.
Attach a bulb.
17

Lamp bulb is blown.

Replace the bulb.
17

The power cord is unplugged.

Plug it securely.
18

14. Lamp bulb blows easily.

The specified bulb is not used.
Replace with a specified bulb.
17

14

SPECIFICATIONS

Item

Specifications

1. Optical system
UIS (Universal Infinity System) optical system

2. Illumination
Built-in illumination system.

6 V, 20 W halogen bulb 6V20WHAL (PHILIPS

7388)

(Average life time: Approx. 100 hr. when used as

directed)

100-120/220-240 V
0.42/0.25A, 50/60 Hz

3. Focusing mechanism
Stage height adjustment mechanism.

Fine adjustment scale: 2.5 m per graduation

Fine adjustment stroke: 0.3 mm per turn

Total stroke: 20 mm.

Pre-focusing knob provided, coarse adjustment knob

tension adjustable.

4. Revolving nosepiece
Quadruple positions fixed (Front oriented)

5. Binocular observation tube

Field number

18

Tube tilting angle

30

Interpupillary distance

48 to 75 mm

adjustment range

6. Stage
Size

120 x 132 mm (with mechanical stage)

Movement range

76 (X-axis) x 30 (Y-axis) mm

Specimen holder

Holds a single specimen.

7. Condenser
Type

Abbe condenser (daylight filter detachable)

N. A .

1.25 (when immersed in oil)

Aperture iris diaphragm

Built in

8. Dimensions & weight

154(W) x 391(H) x 238(D) mm, approx. 5.9 kg

9. Operating environment
Indoor use.

Altitude: Max. 2000 meters

Ambient temperature: 5 to 40C (41 to 104 F)

Maximum relative humidity: 80% for temperatures

up to 31C (88F),

decreasing linearly through 70% at 34C

(93F), 60% at 37C (99F), to 50% relative

humidity at 40C (104F).

Supply voltage fluctuations; Not to exceed 10% of

the normal voltage.

Pollution degree: 2 (in accordance with

IEC60664)

 Installation/Overvoltage category: II (in

accordance with IEC60664)

15

Focal depth : (Object side) : Field number (FN) : Actual field of view : Total power :
(Total magnification)

Resolution :

CX21

OPTICALCHARACTERISTICS
Magnification

Mechanical
tube length
The following table shows the optical characteristics of combinations of eyepieces and objectives. The figure on the
right shows the performance data engraved on the
objectives.
Color band

Number of
aperture

Cover glass
thickness

Objective type (on the back side)

Plan Objective

Optical

10X Eyepieces (FN 18)

Characteristics

W.D.
Cover
Resolution

Power
N.A.

Total
Focal
Actual
Remark

(mm)
Glass
(m)

Depth
Field of

Thickness

Power

Objectives

(m)
View

Plan objective (FN 22)

4X
0.10
18.5

3.36
40X
175.0
4.5

10X
0.25
10.6

1.34
100X
28.0
1.8

40X

0.65
0.6
0.17
0.52
400X
3.04
0.45

100XO
1.25
0.13

0.27
1000X
0.69
0.18
Oil immersed

Legend

Working distance (WD) : Distance between the top surface of cover glass and the objective extremity.

Number of aperture (NA) : The figure corresponding to the F-number of the camera. This is associated with
the resolution and larger NA means higher resolution.

Ability of an objective for identifying adjacent two lines in the image, which is expressed in terms of the
minimum distance between two points on the specimen surface.

The depth range of a specimen, in which focusing is obtained at a time. Stopping down the aperture iris diaphragm
increases the focal depth and increasing the objective NA decreases it. The diameter of the image observed
through an eyepiece, represented in millimeters.

Diameter of the field of view, expressed as the size on the specimen surface. Objective magnification x
Eyepiece magnification.

16

ASSEMBLY

}Each standard set can be assembled by simply attaching the lamp bulb, daylight (blue) filter and power cord.

1 Installing/Replacing the Lamp Bulb

(Fig. 17)

#Before attaching the lamp bulb, remove the parts that may drop

such as the filter and specimen from the microscope frame, and

@
place it on the back so that the bottom can be seen from the front.

1.
Pull the lock knob 1 on the bottom to open the lamp bulb replacement

cover.

2.
Hold the halogen lamp bulb 2 without taking it out of the polyethylene

bag so as not to stain the bulb with fingerprints or stains, and push the

bulb into the pin holes on the socket 3. After attaching, remove the

Fig. 17
polyethylene bag from it.

< Applicable lamp bulb >

6 V, 20 W halogen bulb: 6V20WHAL (Philips Type 7388)

Always use the designated bulb. Using a bulb other than a specified one may lead to a fire hazard.

Fingerprints or stains on the lamp bulb reduce its service life. When it is contaminated, wipe with a cloth slightly moistened with
alcohol.

With the lock knob left in the pulled-out position, close the lamp bulb replacement cover. Then push in the lock knob to lock the cover.

#The cover cannot be closed if the lock knob is in the pushed-in position. Make sure that it is in the pulled-out position before
closing the cover.

Caution for Bulb Replacement During Use or Right After Use

The bulb, lamp socket and areas near these will be extremely hot during and right after use. Set the main switch to

(OFF),

discon-nect the power cord from the wall outlet, then allow the old bulb and lamp socket to cool before replacing the bulb with a
new bulb of the designated type.

2 Mounting the Daylight (Blue) Filter

(Fig. 18)

}This filter modifies the color of observation light into a natural color (day-

@
light color).

Slide the daylight filter 1 into the bottom of the condenser 2 until it

clicks into place.

Fig. 18

17

CX21
| 5

Fig.
20

F
i
g
.
1
9

3
Co
nne
ctin
g
the
Po
wer
Cor
d
(Figs
. 19
& 20)

T
h
e
p
o
w
e
r
c
o
r
d
i
s
v
u
l
n
e
r
a
b
l
e
w
h
e
n
b
e
n
t
o
r
t
w
i
s
t
e
d
.
N
e
v
e
r
s
u
b
j

ect it
to
exce
ssive
force
.
M
ake
sure
that
the
main
switc
h1
is
set
to

(OF
F)
befor
e
connecti
ng
the
pow
er
cord.

Al
ways
use
the
pow
er
cord
provi
ded
by
Oly
mpu
s. If
no
pow
er
cord
is
provi
ded,
plea
se
sele
ct
the
prop
er
pow
er

c
o
r
d
b
y
r
e
f
e
r
r
i
n
g
t
o
t
h
e
s
e
c
t
i
o
n

P
R
O
P
E
R
S
E
L
E
C
T
I
O
N
O
F
T
H
E
P
O
W
E
R

SUP
PLY
COR
D at
the
end
of
this
instr
uctio
n
man
ual.

1.
Con
nect
the
pow
er
cord
s
conn
ector
2 to
conn
ector
3
firml
y.

B
e
sure
to
supp
ly
pow
er
from
a
grou
nded
, 3cond
uctor
pow
er
outle
t
usin
g the
prop
er
pow
er
cord.
If the
pow

e
r
o
u
t
l
e
t
i
s
n
o
t
g
r
o
u
n
d
e
d
p
r
o
p
e
r
l
y
,
O

lymp
us
can
no
long
er
warr
ant
the
elect
rical
safet
y
perfo
rman
ce of
the
equi
pme
nt.

2.
Con
nect
the
pow
er
cord
s
plug
| to a
wall
pow
er
outle
t 5.

18

OPTIONALACCESSORIES

101SystemDiagramofOptionalAccessories

Eyepieces

WHC15X WHC15X-H

Eyepiece micrometer

CH2-FH

Microscope
Frame

CX21

Cord Hanger

Filter Holder

Reflection Mirror
45 mm filter

32.5 mm filter Darkfield Ring

CH20-MM

CH3-CH

CH2-DS

102InstallationandOperationofOptional
Accessories
Fig. 21

Cord Hanger
CH3-CH

@
|

(Figs. 21 & 22)

}When the CH3-CH

cord hanger is
attached on the back
of the micro-scope
frame, the power cord
can be wound around
it for storing.
Align the hooks 2 of
the cord hanger 1
with the cord hanger
mounting position 3,
insert the cord
hanger, push it firmly
against the
microscope frame
and slide it down to
lock it.

microscope may drop and human injury may

result.

Removal

First remove the power cord from the cord

hanger to prevent electric shock.Then,move the
microscope to an edge of the table

#Do not carry the

microscope by
holding it by the cord
hanger. If it is
detached during
transport, the

|. While pushing the cord hanger @ in the

directions 1 and 2 ,

insert the tip of a flat-blade screwdriver 5 or similar tool

into the lower part of the hanger and slide it up to
remove.

Fig. 22

19

Filter Holder
CH2-FH

CX21
Fig. 23

(Fig. 23)

Darkfield Ring
CH2-DS

This accessory accommodates a filter with a

diameter of 32.5 mm or the CH2-DS darkfield
ring.
Remove the daylight filter if it has been
attached.

Push the filter holder 2 containing the desired

filter 1 into the bottom of the condenser until it
clicks into place.

Filter
Application

32.5C
Changes the light of the built-in lamp bulb (which
is yellow-

ish) to natural light.

32.5G533
Reduces fatigue or provides specimen with
contrast.

32.5LB45,

Converts the color of

light for color
photography (with a
day-

For other filters, contact Olympus.

150, 200
light film).

Filters similar to the above having a diameter

of 45 mm are also available for insertion in
the window lens.

(Fig. 24)

Insert the darkfield ring in the CH2-FH filter holder in the same way as inserting
a filter.
The darkfield ring enables darkfield observation using an objective from 4X to
40X.

CH2-DS

Fig. 24

4 Reflection Mirror CH20-MM

(Fig. 25)

}The reflection mirror is designed to be used in microscopic observation in a

location where power supply is not available. It makes it possible to use the
natural light in place of the lamp bulb.

#The microscope should be installed near a window, in a place that

is not exposed to direct sunlight.

The eyepieces should be oriented toward the rear so that the reflec-

tion mirror receives the bright light. Loosen the observation tube

clamping knob and rotate the tube by 180.

1.
Fit the reflection mirror in the window lens of the microscope frame by

aligning the mounting tab.

2.
Remove the daylight filter because it renders the image bluish under

@
natural light.

3.
The reflection mirror 1 should be pointed toward the bright area. While

Fig. 25
observing the image through eyepieces, adjust the orientation of the

reflection mirror.

}A planar reflection mirror is used normally. However, if the image bright-ness is

uneven or the outside view is visible in the image, use a concave reflection
mirror.

20

5 Eyepieces WHC15X/WHC15X-H

(Fig. 26)

}The standard 10X eyepieces are clamped using screws.

1. Using a small flat-blade screwdriver, loosen the clamping screw 1 of a 10X eyepiece and remove it.
2. Insert the WHC15X or WHC15X-H into the eyepiece sleeve and tighten the clamping screw 1.

Fig. 26

6 Using the eyepiece micrometer

(Fig. 27)

}The eyepiece micrometer can be inserted in a standard 10X eyepiece

@
as well as the WHC15X and WHC15X-H.

However, unless the WHC15X-H is used, helicoid adjustment cannot be

performed, so those with poor eyesight will have trouble in bringing the

micrometer into focus.

Get a micrometer with diameter of 19 mm and thickness of 1 mm.

Remove the micrometer sleeve and fit the micrometer @ into the eyepiece with the indication side facing down as
shown in Fig. 27. Replace the micrometer sleeve to use the micrometer.

Fig. 27

21

CX21

PROPER SELECTION OF THE POWER SUPPLY CORD

If no power supply cord is provided, please select the proper power supply cord for the equipment by referring to
Specifications and Certified Cord below:

CAUTION: In case you use a non-approved power supply cord for Olympus products, Olympus can no longer warrant the
electrical safety of the equipment.

Specifications

Voltage Rating
125V AC (for 100-120V AC area) or, 250V AC (for 220-240V AC area)
Current Rating
6A minimum
Temperature Rating
60C minimum
Length
3.05 m maximum
Fittings Configuration
Grounding type attachment plug cap. Opposite terminates in molded-on IEC con-

figuration appliance coupling.

Table 1 Certified Cord

A power supply cord should be certified by one of the agencies listed in Table 1 , or comprised of cordage marked with an
agency marking per Table 1 or marked per Table 2. The fittings are to be marked with at least one of agencies listed in Table 1.
In case you are unable to buy locally in your country the power supply cord which is approved by one of the agencies
mentioned in Table 1, please use replacements approved by any other equivalent and authorized agencies in your country.

Country
Agency
Certification
Country
Agency
Certification

Mark

Mark

Argentina
IRAM

Italy
IMQ

Australia
SAA

Japan
JET, JQA, TV,

UL-APEX / MITI

Austria
VE

Netherlands
KEMA

Belgium
CEBEC

Norway
NEMKO

Canada
CSA

Spain
AEE

Denmark
DEMKO

Sweden
SEMKO

Finland
FEI

Switzerland
SEV

France
UTE

United
ASTA

Kingdom
BSI

Germany
VDE

U.S.A.
UL

Ireland
NSAI

22

Table 2 HAR Flexible Cord

APPROVAL ORGANIZATIONS AND CORDAGE HARMONIZATION MARKING METHODS

Printed or Embossed HarmonizaAlternative

Marking
Utilizing

Approval Organization
tion Marking (May be located on
Black-Red-Yellow Thread (Length

jacket or insulation of internal wirof color section in mm)

ing)

Black

Red

Yellow

Comite Electrotechnique Belge

CEBEC
<HAR>
10

30

10

(CEBEC)

Verband Deutscher Elektrotechniker

<VDE>
<HAR>
30

10

10

(VDE) e.V. Prfstelle

Union Technique de lElectricite

USE
<HAR>
30

10

30

(UTE)

Instituto Italiano del Marchio di

IEMMEQU
<HAR>
10

30

50

Qualita (IMQ)

British Approvals Service for Electric

BASEC
<HAR>
10

10

30

Cables (BASEC)

N.V. KEMA
KEMA-KEUR
<HAR>
10

30

30

SEMKO AB Svenska Elektriska

SEMKO
<HAR>
10

10

50

Materielkontrollanstalter

sterreichischer Verband fr
<VE>
<HAR>
30

10

50

Elektrotechnik (VE)

Danmarks Elektriske Materialkontroll

<DEMKO>
<HAR>
30

10

30

(DEMKO)

National Standards Authority of Ireland

<NSAI>
<HAR>
30

30

50

(NSAI)

Norges Elektriske Materiellkontroll

NEMKO
<HAR>
10

10

70

(NEMKO)

Asociacion Electrotecnica Y
<UNED>
<HAR>
30

10

70

Electronica Espanola (AEE)

Hellenic Organization for

ELOT

<HAR>
30

30

70

Standardization (ELOT)

Instituto Portages da Qualidade

np
<HAR>
10

10

90

(IPQ)

Schweizerischer Elektro
SEV
<HAR>
10

30

90

Technischer Verein (SEV)

Elektriska Inspektoratet
SETI
<HAR>
10

30

90

Underwriters Laboratories Inc. (UL)

SV, SVT, SJ or SJT, 3 X 18AWG

Canadian Standards Association (CSA)

SV, SVT, SJ or SJT, 3 X 18AWG

23

Shinjuku Monolith, 3-1, Nishi Shinjuku 2-chome, Shinjuku-ku, Tokyo, Japan

Postfach 10 49 08, 20034, Hamburg, Germany
2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A.

491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373
2-8 Honduras Street, London EC1Y OTX, United Kingdom.
31 Gilby Road, Mt. Waverley, VIC 3149, Melbourne, Australia. 6100 Blue Lagoon
Drive, Suite 390 Miami, FL 33126-2087, U.S.A.

Printed in China 2005 04 M 1501