PHILIPPINE ASSOCIATION OF SCHOOLS OF MEDICAL TECHNOLOGY AND PUBLIC HEALTH

Prototype Course Syllabus

Course Title: Microbiology 1 (Bacteriology)

Course Description:

The course covers the morphology and physiology of bacteria, rickettsiae and other significant atypical bacteria and their role in infection and immunity. Emphasis is made on
their isolation and identification and susceptibility testing as an aid in laboratory diagnosis.

Course Credit: 5 units (3 units lecture, 2 units laboratory)

Contact Hours: 3 hours lecture and 6 hours laboratory per week (54 hours lecture and 108 hours laboratory per semester)

Prerequisites: Biochemistry, Human Anatomy and Physiology

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Placement: Third Year, 1 Semester

Terminal Competencies: At the end of this course, the student is able to:
1. identify bacteria according to standard laboratory techniques: microscopic and staining; growth and biochemical characteristics; and antigen typing.
2. perform appropriate antimicrobial susceptibility testing
3. operate common instruments used in Bacteriology
4. participate in quality assurance/quality control program in Microbiology
5. understand the duties and responsibilities of medical technologists in the clinical setting
6. perform proper specimen collection, processing, transport and waste disposal
7. apply concepts in controlling the growth and spread of pathogenic bacteria

References:
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1. Alcamo, Edward I. Fundamentals of Microbiology 6 ed. Boston: Jones & Bartlett Publishers, 2001.
2. Bartlet, Margaret A. Diagnostic Bacteriology: A Study Guide. F.A. Davis Co. Philadelphia, USA, 2000.
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3. Black, Jacquelyn. Microbiology: Principles and Explorations 7 ed. USA: Wiley, 2008.
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4. Brooks, Geoff. Jawetz, Melnick & Adelberg’s Medical Microbiology 24 ed. Boston: Mc Graw Hill, 2007.
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5. Burton, Gwendolyn and Paul Engelkirk. Burton’s Microbiology for the Health Sciences 8 ed. USA: Lippincott Williams and Wilkins, 2007.
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6. Forbes, Betty, Daniel Salm and Alice Weissfield. Bailey & Scott’s Diagnostic Microbiology 12 ed. USA: Mosby, 2007.

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 7. Funke, Berdell. Microbiology: An Introduction. USA: Addison Wesley Publishing Co., 2007.
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8. Gladwin, Mark and Bill Trattler. Clinical Microbiology Made Ridiculously Simple 4 ed. USA: MedMaster, Inc., 2007.
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9. Hawkey, Peter and Dierdre Lewis. Medical Bacteriology: A Practical Approach 2 ed. USA: Oxford University Press, 2004.
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10. McClatchey, Kenneth. Clinical Laboratory Medicine 2 ed. USA: Lippincott Williams and Wilkins, 2002.
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11. Mcpherson, Richard A. and Matthew R. Pincus. Henry’s Clinical Diagnosis and Management by Laboratory Methods 21 ed. Philadelphia: Elsevier Inc., 2007.
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12. Murray, Patrick R. et. al. Medical Microbiology 5 ed. USA: Mosby, 2005.
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13. Pommerville, Jeffrey. Alcamo’s Fundamentals of Microbiology 8 ed. USA: Jones and Bartlett Publishers, 2006.
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14. Presscott, L., J. Harley and D. Klein. Microbiology 6 ed. USA: McGraw-Hill Science, 2004.
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15. Tortora, G., B. Funke and C. Case. Microbiology: An Introduction 9 ed. USA: Benjamin Cummings, 2006.
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16. Vandepitte J., J. Verhaegen and K. Engbaek. Basic Laboratory Procedures in Clinical Bacteriology 2 ed. World Health Organization, 2003.

Electronic References:
1. http://connection.lwww.com/go/burton/7e
2. http://medic.med.uth.tmc.edu/path/00001450.htm
3. http://www.kensbiorefs.com/Microbio.html
4. http://www.microbes.info
5. http://www.splammo.net/JLbactsite.html

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 Learning Objectives / T.A. Teaching Strategies Skills Values Evaluation/Assessment
Intermediate Competencies Content
Lecture Laboratory
At the end of this unit, the student is I. Introduction to Microbiology Large group Demonstration & Technical Skills: Adherence to Oral and Written Exam
able to: Lecture: 2 discussion return demonstration Use of rules Participation during
3. Define terms. 1. Definition of terms: hrs PowerPoint Performance of tests Microscope Responsibility discussions
4. Enumerate the development of 1.1. Bacteriology, bacteria, presentation Safety Accountability Quality of assignment
science with emphasis on pathogenecity, virulence etc. precautions in Cooperation submitted
person/scientists and their 2. Historical Development the laboratory Performance checklist
contributions 3. Divisions of Microbiology Specimen
5. Explain the divisions of handling
Microbiology Laboratory: 6 Waste disposal
6. Discuss general considerations 1. Expectations/requirements Microbiology hrs
regarding proper handling, Laboratory:
processing, storage of 2. Rules to follow in handling, processing,
specimens and disposal of disposal of specimens/waste in
wastes Microbiology laboratory
7. Discuss the roles of a medical 3. Barrier precautions
technologist in a microbiology 4. Proper use of different apparatus,
laboratory materials and equipment: Petri Dish,
inoculating loop and needle
5. Review use and care of microscope
At the end of this unit, the student is II. Laboratory Safety and Infection Control Lecture with Performance of Technical Skills Honesty Oral and Written Exam
able to: Lecture: 3 demonstration Tests Multi-tasking Commitment Participation during
1. Define terms 1. Sterilization hrs Discussion Demonstration and Responsibility discussions
2. Discuss concepts, principles& 1.1. Physical (Heat) Board work return demonstration Accountability Quality of assignment
significance of infection control 1.1.1. Autoclave Case Adherence to submitted
& laboratory safety 1.1.2. Direct Heat Presentation rules Performance checklist
3. Discuss various methods of 1.1.3. Oven Cooperation Practical Exam
sterilization & disinfections with 1.1.4. Incineration Teamwork
emphasis on temp, time, 1.1.5. Pasteurization Concern for
principles/mechanism involved, 1.1.6. Boiling others and the
when to use; advantages & 1.2. Filtration environment
disadvantages 2. Disinfection
4. Classify the different biosafety 2.1. Chemicals:
cabinets 2.2. Alcohol
5. Explain the significance of 2.3. Chlorine
pathogenic and virulence 2.4. Others
factors in the development of 3. Antimicrobial agents
infections 4. Antimicrobial Susceptibility testing
6. Perform correctly sterilization & 4.1. Automated
disinfection practices 4.2. Diffusion
7. Explain the concepts behind 4.3. Dilution
Antimicrobial Susceptibility 5. Use of bio-safety cabinets
testing 6. Barrier precautions
8. Differentiate between the two 7. Control of nosocomial infections

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 types of antimicrobial
susceptibility testing Laboratory: 6
9. Discuss significance 1. Sterilization: hrs
advantages and disadvantages 1.1. Autoclaving
of each method of sensitivity 1.2. Direct heating
test 1.3. Filtration
10. Interpret test results 2. Cleaning of working area with Clorox &
11. Explain the concepts of other chemicals
nosocomial infections 3. Proper hand washing techniques
12. Outline the roles of med tech in 4. Lab exercises on the effects of chemicals
prevention & control of & temperature on bacteria
nosocomial infections 5. Proper working attire in a Bacteriology
laboratory
At the end of this unit, the student is III. Bacterial Structure and Function, Growth Computer-aided Actual performance Technical Skills Honesty Oral and Written Exam
able to: and Nutrition Lecture Demonstration/return Multi-tasking Commitment Participation during
1. Describe bacteria according to: Lecture: 3 /Discussion demonstration Problem-Solving Responsibility discussions
physiology, replication, 1. Characteristics of Bacteria according to: hrs Case studies Discussion of test Skills Accountability Quality of assignment
morphology, classification 1.1. Physiology: Structure, Function Problem solving results Communication Adherence to submitted
2. Identify the growth requirements 1.2. Bacterial morphology Making conclusions skills rules Performance checklist
for each particular group of 1.3. Classification & guidelines Cooperation Practical Exam
bacteria 1.4. Replication Multi-tasking Teamwork
3. Illustrate and discuss the growth 1.5. Growth requirements
curve with emphasis on the 1.5.1. Physical
events happening per stage 1.5.2. Nutritional
4. Discuss specimen collection, 2. Bacterial Growth Curve
transport, storage, and 3. Bacterial Identification
processing 3.1. Microscopy: Morphology
5. Perform correctly specimen 3.2. Cultural/Colony Characteristics
collection: Swabs, Nasal, 3.3. Staining Characteristics
Throat, Wound, Urine; transport 3.4. Biochemical
& labeling, processing & waste 3.5. Serological: Antigen testing
disposal 3.6. Molecular Techniques
6. Correlate specimen with 4. Quality Assurance-Quality Control
common pathogens isolated
7. Perform correctly hanging drop Laboratory: 9
and wet mount techniques. 1. Acceptable specimens for bacterial hrs
8. Describe the results of hanging identification
drop and wet mounts 1.1. Swabs
9. Discuss concepts and principles 1.2. Blood
of the techniques by which 1.3. Urine
bacteria are identified in the 1.4. Stool
laboratory 1.5. Tissue
10. Correlate methods with test 1.6. Aspirates
results 1.7. Others
11. Prepare a general schema for 2. Specimen collection, handling, transport,
identification of bacteria in the processing and preservation
laboratory 3. Hanging Drop Method and Wet Mounts

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12. Describe microscopic 4. Role of Microscopy in Bacterial
morphology Identification
13. Identify correctly bacterial 4.1. Bright field
reaction to staining and 4.2. Phase contrast
morphology 4.3. Electron
14. Describe staining and 4.4. Dark-field
morphological characteristics 4.5. Fluorescent
15. Differentiate staining techniques 5. Preparation of Bacterial Smear for
according to: stains used, staining from:
purpose, principles, 5.1. Directly from specimen (throat swab
advantages, disadvantages, or any swab)
and procedures 5.2. Colonies growing in culture media
16. Perform laboratory exercises 6. Staining Techniques:
correctly 6.1. Simple stain
17. Write a report based on 6.2. Differential stain
accepted standard format 6.2.1. Gram stain
6.2.2. Acid fast stain
6.3. Special stains
6.4. Others
7. Laboratory Safety
8. Laboratory Waste Management
At the end of this unit, the student is IV. Bacterial Genetics Computer-aided Multi-tasking Honesty Oral and Written Exam
able to: Lecture: 2 Lecture Problem-Solving Commitment Participation during
1. Discuss the concepts of 1. Basic Concepts hrs /Discussion Skills Responsibility discussions
bacterial genetics and its 1.1. Bacterial DNA & RNA Case studies Presentation Accountability Quality of assignment
importance in the study of 1.2. Plasmids & Microbial Resistance Problem solving Skills Adherence to submitted
bacteria. 1.3. DNA exchange in bacteria: Output Time rules
2. Appreciate the role of medical 1.3.1. Transformation presentation management Cooperation
technologists in the control and 1.3.2. Transduction and critiquing Communication Teamwork
prevention of microbial 1.3.3. Conjugation skills Prudence
resistance
At the end of this unit, the student is V. Pathogenesis and Epidemiology Computer-aided Multi-tasking Honesty Oral and Written Exam
able to: Lecture: 2 Lecture Problem-Solving Commitment Participation during
1. Define terms. 1. Basic Concepts hrs /Discussion Skills Responsibility discussions
2. Discuss the mode of 2. Mode of Transmission Case studies Presentation Accountability Quality of assignment
transmission of bacterial 3. Factors associated with pathogenecity Problem solving Skills Adherence to submitted
infections and virulence Time rules
3. Correlate factors of 3.1. Toxins management Cooperation
pathogenecity and virulence 3.2. Enzymes Communication Teamwork
3.3. Capsules skills Prudence
3.4. Others
At the end of this unit, the student is VI. Normal Flora Large group Demonstration & Technical Skills: Adherence to Oral and Written Exam
able to: Lecture: 2 discussion return demonstration Use of rules Participation during
1. Define terms 1. Basic Concepts hrs PowerPoint Performance of tests Microscope Responsibility discussions
2. Identify body sites with normal 2. Body Sites with Normal Flora presentation Safety Accountability Quality of assignment
flora 3. Predominant flora precautions in Cooperation submitted
3. Discuss the predominant normal 4. Beneficial and harmful effects of normal the laboratory Performance checklist

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 flora in each body site flora Specimen
4. Explain the beneficial and handling
harmful effects of normal flora Laboratory 6 Waste disposal
1. Isolation of normal flora hrs
2. Laboratory Safety
3. Laboratory Waste Management
At the end of this unit, the student is VII. Micrococcaceae and Streptococcaeae Lecture with Performance of Technical Skills: Honesty Oral and Written Exam
able to: 1. Micrococcaceae demonstration Tests Use of Commitment Participation during
1. Describe Micrococcaceae & 1.1. Staphylococcus Multimedia Demonstration and Microscope Responsibility discussions
Streptococcus in terms of their 1.2. Micrococcus presentation return demonstration Safety Accountability Quality of assignment
general properties and 2. Streptococcaceae Discussion precautions in Adherence to submitted
pathogenesis 2.1. Streptococcus Board work the laboratory rules Performance checklist
2. Discuss the identification 2.2. Entererococcus Case Studies Specimen Cooperation Practical Exam
characteristics of handling Teamwork
Micrococaceae in terms of Lecture: 3.5 Waste disposal Concern for
morphology, culture media, 1. General characteristics hrs others and the
colony characteristics, 2. Pathogenesis & spectrum of disease environment
biochemical test, and 3. Laboratory Identification
serological test 3.3. Specimen used for Isolation
3. Explain the principles/concepts 3.4. Morphology & Staining Reaction
of different identification 3.5. Culture media and colony
techniques used characteristics
4. Different pathogenic from non- 3.6. Biochemical tests
pathogenic members of the 3.7. Antigen typing/serology
genus 4. Prevention and control
5. Enumerate the appropriate
specimen submitted in the Laboratory: 6
laboratory for isolation 1. Culture media hrs
6. Prepare a schematic diagram 1.1. classification
for identification 1.2. composition
7. Classify culture media 1.3. preparation
according to: indications for use, 1.4. storage
contents, purpose, etc. 1.5. quality assurance/control
8. Prepare culture media 1.5.1. spore strip test
9. Perform quality control of 1.5.2. sterility test
culture media before use. 2. Preparation of culture media
10. Identify Staphylococcus, 2.1. Nutrient Agar
Micrococcus and Streptococcus 2.2. Blood Agar Plate
from specimens 2.3. Chocolate Agar Plate
3. Identification of Staphylococcus and
Streptococcus from specimens, prepared
slides and visual aids
4. Laboratory Safety
5. Laboratory Waste Management
At the end of this unit, the student is VIII. Neisseriaceae Lecture with Performance of Technical Skills: Honesty Oral and Written Exam
able to: Lecture: demonstration Tests Use of Commitment Participation during
1. Describe the members of 1. Neisseriaceae Multimedia Demonstration and Microscope Responsibility discussions

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 Neisseriaceae in terms of their 1.1. Pathogenic Neisseria presentation return demonstration Safety Accountability Quality of assignment
general properties and 1.2. Non-pathogenic Neisseria Discussion precautions in Adherence to submitted
pathogenesis 1.3. Moraxella catarrhalis Board work the laboratory rules Performance checklist
2. Discuss the identification Case Studies Specimen Cooperation Practical Exam
characteristics of Lecture: handling Teamwork
Neisseriaceae in terms of 1. General characteristics 3.5 Waste disposal Concern for
morphology, culture media, 2. Pathogenesis & spectrum of disease hrs others and the
colony characteristics, 3. Laboratory Identification environment
biochemical test, and 3.1. Specimen used for Isolation
serological test 3.2. Morphology & Staining Reaction
3. Explain the principles/concepts 3.3. Culture media and colony
of different identification characteristics
techniques used 3.4. Biochemical tests
4. Different pathogenic from non- 3.5. Antigen typing/serology
pathogenic members of the 4. Prevention and control
genus
5. Enumerate the appropriate Laboratory: 6
specimen submitted in the 1. Identification techniques for: hrs
laboratory isolation & 1.1. Pathogenic Neisseria
identification 1.2. Non-pathogenic Neisseria
6. Prepare a schematic diagram 1.3. Moraxella catarrhalis
for identification 2. Identification of species using prepared
7. Classify culture media slides and visual aids
according to: indications for use, 3. Laboratory Safety
contents, purpose, etc. 4. Laboratory Waste Management
8. Perform correctly inoculation
techniques and tests
9. Summarize, based on results of
identification techniques,
bacterial flora in a human
person and environment
At the end of this unit, the student is IX. Enterobacteriaceae Lecture with Performance of Technical Skills: Honesty Oral and Written Exam
able to: 1. True Intestinal Pathogens demonstration Tests Use of Commitment Participation during
1. Discuss the process of 1.1. Salmonella Multimedia Demonstration and Microscope Responsibility discussions
identification of 1.2. Shigella presentation return demonstration Safety Accountability Quality of assignment
Enterobacteriaceae in terms of 1.3. Yersinia Discussion precautions in Adherence to submitted
morphology, culture media, 1.4. Escherichia Board work the laboratory rules Performance checklist
colony characteristics, 2. Opportunistic pathogens Case Studies Specimen Cooperation Practical Exam
biochemical test, serological 2.1. Escherichia handling Teamwork
test 2.2. Citrobacter Waste disposal Concern for
2. Explain the principles/concepts 2.3. Proteus others and the
of different identification 2.4. Edwardsiella environment
techniques used 2.5. Morganella
3. Different pathogenic from non- 2.6. Hafnia
pathogenic members of the 2.7. Providencia
genus 2.8. Enterobacter
4. State the pathogenic and 2.9. Serratia

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 virulence factors present in 2.10. Klebsiella.
bacteria belonging to the group 2.11. Others
and disease caused by them
5. Enumerate the appropriate Lecture: 3.5
specimen submitted in the 1. General characteristics hrs
laboratory isolation & 2. Pathogenesis & spectrum of disease
identification 3. Laboratory Identification
6. Prepare a schematic diagram 3.1. Specimen used for Isolation
for identification 3.2. Morphology & Staining Reaction
7. Classify culture media 3.3. Culture media and colony
according to: indications for use, characteristics
contents, purpose, etc. 3.4. Biochemical tests
8. Properly inoculate specimen on 3.5. Antigen typing/serology
culture media 4. Prevention and control
9. Summarize, based on results of
identification techniques, Laboratory: 6
bacterial flora in a human 1. Isolation and identification of members of hrs
person and environment Enterobacteriaceae using urine or stool
10. Perform identification 2. Identification of species using prepared
techniques slides and visual aids
11. Identify the isolate 3. Laboratory Safety
4. Laboratory Waste Management
At the end of this unit, the student is X. Non-enteric Gastrointestinal Pathogens Lecture with Performance of Technical Skills: Honesty Oral and Written Exam
able to: 1. Vibrio demonstration Tests Use of Commitment Participation during
1. Discuss the process of 2. Campylobacter Multimedia Demonstration and Microscope Responsibility discussions
identification of the members of 3. Helicobacter presentation return demonstration Safety Accountability Quality of assignment
the non-enteric gastrointestinal 4. Aeromonas Discussion precautions in Adherence to submitted
pathogens in terms of 5. Pleisomonas Board work the laboratory rules Performance checklist
morphology, culture media, 6. Others Case Studies Specimen Cooperation Practical Exam
colony characteristics, handling Teamwork
biochemical test (plus positive Lecture: 3.5 Waste disposal Concern for
and negative results), 1. General characteristics hrs others and the
serological test 2. Pathogenesis & spectrum of disease environment
2. Explain the principles/concepts 3. Laboratory Identification
of different identification 3.1. Specimen used for Isolation
techniques used 3.2. Morphology & Staining Reaction
3. Different pathogenic from non- 3.3. Culture media and colony
pathogenic members of the characteristics
genus 3.4. Biochemical tests
4. Enumerate the appropriate 3.5. Antigen typing/serology
specimen submitted in the 4. Prevention and control
laboratory isolation &
identification Laboratory: 6
5. Construct a schematic diagram 1. Isolation and identification of members of hrs
for identification. non-enteric gastrointestinal pathogens
2. Identification of species using prepared
slides and visual aids

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 3. Laboratory Safety
4. Laboratory Waste Management
At the end of this unit, the student is XI. Non-fermentative Gram Negative Bacilli Lecture with Performance of Technical Skills: Honesty Oral and Written Exam
able to: and Aerobic Gram Positive Bacilli demonstration Tests Use of Commitment Participation during
1. Discuss the process 1. Non-fermentative Gram Negative Bacilli Multimedia Demonstration and Microscope Responsibility discussions
identification of the non- 1.1. Pseudomonas presentation return demonstration Safety Accountability Quality of assignment
fermentative group in terms of 1.2. Acinetobacter Discussion precautions in Adherence to submitted
morphology, culture media, 1.3. Flavobacterium Board work the laboratory rules Performance checklist
colony characteristics, 1.4. Burkholderia Case Studies Specimen Cooperation Practical Exam
biochemical test(plus positive 1.5. Alcaligenes handling Teamwork
and negative results), 1.6. Achromobacter Waste disposal Concern for
serological test 1.7. Stenotrophomonas others and the
2. Explain the principles/concepts 1.8. Others environment
of different identification 2. Aerobic Gram Positive Bacilli
techniques used 2.1. Bacillus
3. Different pathogenic from non- 2.2. Listeria
pathogenic members of the 2.3. Corynebacterium
genus 2.4. Erysipelothrix
4. Enumerate the appropriate 2.5. Nocardia
specimen submitted in the 2.6. Streptomyces
laboratory isolation & 2.7. Others
identification
5. Prepare a schematic diagram Lecture: 3.5
for identification 1. General characteristics hrs
6. Perform correctly inoculation 2. Pathogenesis & spectrum of disease
techniques and tests 3. Laboratory Identification
3.1. Specimen used for Isolation
3.2. Morphology & Staining Reaction
3.3. Culture media and colony
characteristics
3.4. Biochemical tests
3.5. Antigen typing/serology
4. Prevention and control

Laboratory: 6
1. Isolation and identification of members of hrs
non-fermentative gram negative bacilli
and aerobic gram positive bacilli
2. Identification of species using prepared
slides and visual aids
3. Laboratory Safety
4. Laboratory Waste Management
At the end of this unit, the student is XII. Small, Pleomorphic Gram Negative Bacilli Lecture with Performance of Technical Skills: Honesty Oral and Written Exam
able to: 1. Hemophilus demonstration Tests Use of Commitment Participation during
1. Discuss the process of 2. Bordetella Multimedia Demonstration and Microscope Responsibility discussions
identification of small 3. Brucella presentation return demonstration Safety Accountability Quality of assignment
pleomorphic gram negative 4. Francisella Discussion precautions in Adherence to submitted

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 bacilli and the aerobic gram 5. Gardnerella Board work the laboratory rules Performance checklist
positive bacilli in terms of 6. Legionella Case Studies Specimen Cooperation Practical Exam
morphology, culture media, 7. Others handling Teamwork
colony characteristics, Waste disposal Concern for
biochemical test (plus positive Lecture: 3.5 others and the
and negative results), 1. General characteristics hrs environment
serological test 2. Pathogenesis & spectrum of disease
2. Explain the principles/concepts 3. Laboratory Identification
of different identification 3.1. Specimen used for Isolation
techniques used 3.2. Morphology & Staining Reaction
3. Different pathogenic from non- 3.3. Culture media and colony
pathogenic members of the characteristics
genus 3.4. Biochemical tests
4. State the pathogenic and 3.5. Antigen typing/serology
virulence factors present in 4. Prevention and control
bacteria belonging to the group
and disease caused by them Laboratory: 6
5. Determine the appropriateness 1. Isolation and identification of members of hrs
of the different specimens small, pleomorphic gram negative bacilli
submitted in the laboratory for 2. Identification of species using prepared
isolation & identification slides and visual aids
6. Prepare a schematic diagram 3. Laboratory Safety
for identification 4. Laboratory Waste Management
7. To distinguish the species of
the aerobic gram positive bacilli
from other bacteria
8. Identify members from
submitted sample
At the end of this unit, the student is XIII. Mycobacterium Group Lecture with Performance of Technical Skills: Honesty Oral and Written Exam
able to: 1. Mycobacterium tuberculosis Complex demonstration Tests Use of Commitment Participation during
1. Discuss the process of 2. Non-tuberculous Mycobacteria Multimedia Demonstration and Microscope Responsibility discussions
identification of Mycobacteria in 3. Mycobacterium leprae presentation return demonstration Safety Accountability Quality of assignment
terms of morphology, culture Discussion precautions in Adherence to submitted
media, colony characteristics, Lecture: 3.5 Board work the laboratory rules Performance checklist
biochemical test (plus positive 1. General characteristics hrs Case Studies Specimen Cooperation Practical Exam
and negative results), 2. Pathogenesis & spectrum of disease handling Teamwork
serological test 3. Laboratory Identification Waste disposal Concern for
2. Explain the principles/concepts 3.1. Specimen used for Isolation others and the
of different identification 3.2. Morphology & Staining Reaction environment
techniques used 3.3. Culture media and colony
3. Different pathogenic from non- characteristics
pathogenic members of the 3.4. Biochemical tests
genus 3.5. Antigen typing/serology
4. State the pathogenic and 4. Prevention and control
virulence factors present in 4.1. TB-DOTS
bacteria belonging to the group
and disease caused by them Laboratory: 6

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5. Determine the appropriateness 1. Isolation and identification of members of hrs
of the different specimens Mycobacteria
submitted in the laboratory for 1.1. Culture
isolation & identification 1.2. Staining
6. Prepare a schematic diagram 1.2.1. Acid Fast Staining
for identification 1.2.2. Other Staining Techniques
7. To distinguish the species 1.3. Others
8. Identify members from of the 2. Identification of species using prepared
gram positive bacilli from other slides and visual aids
bacteria submitted sample 3. Laboratory Safety
4. Laboratory Waste Management
At the end of this unit, the student is XIV. Anaerobic Bacteria Lecture with Performance of Technical Skills: Honesty Oral and Written Exam
able to: 1. Clostridium demonstration Tests Use of Commitment Participation during
1. Enumerate the characteristics of 2. Bacteroides Multimedia Demonstration and Microscope Responsibility discussions
the anaerobic bacteria in terms 3. Actinomyces presentation return demonstration Safety Accountability Quality of assignment
of metabolism/physiology 4. Fusobacterium Discussion precautions in Adherence to submitted
2. Explain precautions of the 5. Peptostreptococcus Board work the laboratory rules Performance checklist
collection, processing, handling, 6. Porphyromonas Case Studies Specimen Cooperation Practical Exam
disposal of specimen 7. Bifidobacterium handling Teamwork
3. Discuss the clinically 8. Lactobacillus Waste disposal Concern for
significance anaerobic in terms 9. Veilonella others and the
of morphology, isolation 10. Megasphaera environment
techniques, biochemical tests 11. Acidominococcus
4. Perform the set-up of anaerobic 12. Others
bacteriology
5. Discuss the importance of the Lecture: 3.5
components in the set-up 1. General characteristics hrs
2. Pathogenesis & spectrum of disease
3. Laboratory Identification
3.1. Specimen collection and processing
3.2. Anaerobic set-up and culture media
4. Prevention and control

Laboratory: 6
1. Isolation and identification of members of hrs
anaerobic bacteria
1.1. Anaerobic culture
1.2. Anaerobic set-up
1.3. Microscopic identification
2. Identification of species using prepared
slides and visual aids
3. Laboratory Safety
4. Laboratory Waste Management
At the end of this unit, the student is XV. Spirochetes Lecture with Performance of Technical Skills: Honesty Oral and Written Exam
able to: 1. Treponema demonstration Tests Use of Commitment Participation during
1. Discuss the process of 2. Borrelia Multimedia Demonstration and Microscope Responsibility discussions
identification of medically 3. Leptospira presentation return demonstration Safety Accountability Quality of assignment

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 important Spirochetes Discussion precautions in Adherence to submitted
2. Correlate appropriate specimen Lecture: 3 Board work the laboratory rules Performance checklist
with isolates 1. General characteristics hrs Case Studies Specimen Cooperation Practical Exam
3. Characterize the organisms 2. Pathogenesis & spectrum of disease handling Teamwork
according to morphology and 3. Laboratory Identification Waste disposal Concern for
pathophysiology 4. Prevention and control others and the
4. Perform the set-up of anaerobic environment
bacteriology Laboratory: 6
5. Discuss the importance of the 1. Staining techniques for Spirochetes hrs
components in the set-up 2. Identification of species using visual aids
6. Perform correctly staining 3. Laboratory Safety
techniques for Spirochetes 4. Laboratory Waste Management
7. Describe Spirochetes observed
under the microscope
At the end of this unit, the student is XVI. Unique Bacteria Lecture with Performance of Technical Skills: Honesty Oral and Written Exam
able to: 1. Rickettsiaceae demonstration Tests Use of Commitment Participation during
1. Discuss the process of 3.1. Rickettsia Multimedia Demonstration and Microscope Responsibility discussions
identification of unique bacteria 3.2. Ehrlichia presentation return demonstration Safety Accountability Quality of assignment
2. Explain the pathogenesis and 3.3. Orientia Discussion precautions in Adherence to submitted
spectrum of diseases caused by 4. Mycoplasmataceae Board work the laboratory rules Performance checklist
unique bacteria 4.1. Mycoplasma Case Studies Specimen Cooperation Practical Exam
3. Discuss the mode of 4.2. Ureaplasma handling Teamwork
transmission of the 5. Chlamydiaceae Waste disposal Concern for
microorganisms 5.1. Chlamydia others and the
4. Explain the principles behind 5.2. Chlamydophila environment
the tests employed to identify
unique bacteria Lecture: 3
5. Perform tests to identify unique 1. General characteristics hrs
bacteria 2. Pathogenesis & spectrum of disease
3. Laboratory Identification
4. Prevention and control

Laboratory: 6
1. Laboratory work-up on the identification of hrs
unique bacteria
At the end of this unit, the student is XVII. Miscellaneous Bacteria Lecture with Performance of Technical Skills: Honesty Oral and Written Exam
able to: 1. Eikinella demonstration Tests Use of Commitment Participation during
1. Discuss the process of 2. Kingella Multimedia Demonstration and Microscope Responsibility discussions
identification of miscellaneous 3. Streptobacillus presentation return demonstration Safety Accountability Quality of assignment
bacteria 4. Bartonella Discussion precautions in Adherence to submitted
2. Explain the pathogenesis and 5. Spirillum Board work the laboratory rules Performance checklist
spectrum of diseases caused by 6. Others Case Studies Specimen Cooperation Practical Exam
miscellaneous bacteria handling Teamwork
3. Discuss the mode of Lecture: 3 Waste disposal Concern for
transmission of the 1. General characteristics hrs others and the
microorganisms 2. Pathogenesis & spectrum of disease environment
4. Explain the principles behind 3. Laboratory Identification

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 the tests employed to identify 4. Prevention and control
miscellaneous bacteria
5. Perform tests to identify Laboratory: 6
miscellaneous bacteria 1. Isolation and identification of members of hrs
miscellaneous bacteria
2. Identification of species using prepared
slides and visual aids
At the end of this unit, the student is XVIII. Applied Bacteriology Lecture with Performance of Technical Skills: Honesty Oral and Written Exam
able to: Lecture: 3 demonstration Tests Use of Commitment Participation during
1. Prepare media for the finale 1. Applied Bacteriology hrs Multimedia Demonstration and Microscope Responsibility discussions
examination 1.1. Water Bacteriology presentation return demonstration Safety Accountability Quality of assignment
2. Perform correctly the 1.2. Food Bacteriology Discussion precautions in Adherence to submitted
procedures in water, food and 1.3. Milk Bacteriology Board work the laboratory rules Performance checklist
milk analysis 1.4. Environmental Bacteriology Case Studies Specimen Cooperation Practical Exam
3. Interpret the results of the handling Teamwork
analysis Laboratory: 15 Waste disposal Concern for
1. Lab. Identification of Unknown Bacteria hrs others and the
as Practical Examination environment
2. Laboratory Safety
3. Laboratory Waste Management

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 Technical Working Group for Prototype Course Syllabi Development
(2007-2008)

Zennie Aceron Frederick Llanera Anacleta Valdez

Petrona Benitez Carina Magbojos Rowen Yolo
Sergia Cacatian Gregorio Martin
Zenaida Cajucom Fe Martinez and other PASMETH members
Edwin Cancino Josephine Milan not cited in this page who in
Jacinta Cruz Ferdinand Mortel one way or another has
De Carlos Leon Magdalena Natividad contributed greatly to the
success of this endeavor…
Oliver Shane Dumaoal Rodolfo Rabor
Bernard Ebuen Ma. Teresa Rodriguez
Nini Lim Celia Roslin

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