Lecture 8 & 9: Cell Structure and Function

Much of the diversity of forms and functions in living organisms results from small atoms being combined
in different ways to form a number of molecules and molecules form macromolecules. Eventually, these
macromolecules build cells, tissues, organs and finally, an entire organism.

Cells
A cell is the smallest unit of life that can survive and reproduce on its own, given
information in DNA, energy, and raw materials. Some cells live and reproduce
independently. Others do so as part of a multicelled organism.

Discovery of cells
In the middle of the 17th century, one of the pioneers of microscopy, Robert
Hooke (16351703), decided to examine a piece of cork tissue with his home-built
microscope. He saw numerous box shaped structures that he thought resembled
row of empty boxes or rooms, so he called them cells.

Cell theory
Matthias Schleiden and Theodor Schwann, hypothesized that a plant cell is an independent living unit even
when it is part of a plant and both concluded that the tissues of animals as well as plants are composed of
cells and their products. Together, the two scientists recognized that cells have a life of their own even
when they are part of a multicelled body.
Later, physiologist Rudolf Virchow realized that all cells he studied descended from another living cell.
These and many other observations yielded three generalizations that today constitute the cell theory:

1) Every organism is composed of one or more cells

2) Cell is smallest unit having properties of life
3) Continuity of life arises from growth and division of single cells

Thus, Cell theory is that all organisms consist of one or more cells, which are the basic unit of life.

Cell Properties
A cell is the smallest unit that shows the properties of life.
These properties include -
Can survive on its own or has potential to do so
Is highly organized for metabolism
Senses and responds to environment
Has potential to reproduce

Cell types
Biologists have categorized cells into two general types: eukaryotic and prokaryotic cells.
The cells of plants, animals, fungi, protozoa, and algae are eukaryotic, and are placed in a category called
Eucarya . All eukaryotic cells have their genetic material surrounded by a nuclear membrane forming the
cellular nucleus. They also have a large number and variety of complex organelles, each specialized in the
metabolic function it performs. In general, they are large in comparison to Prokaryotic cells. These cell
types do not have a nuclear membrane; therefore they lack a cellular nucleus. In addition, they display
unique chemical and metabolic characteristics but do not have the variety and number of organelles seen
in eukaryotes.
Eukaryotic Cells

Prokaryotic Cell
 Prokaryotic Cell Eukaryotic Cell

1. Generally small (1-10 m) in size 1. Generally large (5-100 m). Eukaryotic cells
and volume are about 15 times the size of a typical
prokaryote and can be as much as 1000 times
greater in volume.

2. Cell wall is present 2. Cell walls may or may not be present.

3. Nucleus is absent 3. Nucleus is present

4. Prokaryotic cell division occurs 4. Mitosis, including mitotic spindle, centrioles

through fission or budding, no in many species.
mitosis occurs.

5. Prokaryotes generally lack 5. Mitochondria and chloroplasts are present in

membrane-bound cell Eukaryotes.
compartments: such as mitochondria
and chloroplasts.

6. Single circular chromosome 6. Multiple linear chromosomes

7. Chromosome found in a 7. Chromosomes found in a membrane-bound

cytoplasmic region called the nucleus.
nucleoid.

8. No internal membranes 8. Extensive network of internal membranes,

Some infolded plasma membrane, Complex, with microtubules, intermediate
No Cytoskeleton filaments and actin filaments

9. Intracellular movement is absent 9. Cytoplasmic streaming, endocytosis,

phagocytosis, mitosis, vesicle transport.

Cell size

Almost all cells are too small to see with the naked eye. Why? The answer begins with the processes that
keep a cell alive. A living cell must exchange substances with its environment at a rate that keeps pace
with its metabolism. These exchanges occur across the plasma membrane, which can handle only so many
exchanges at a time. Thus, cell size is limited by a physical relationship called the surface-to-volume ratio.
By this ratio, an objects volume increases with the cube of its diameter, but its surface area increases
only with the square. If the cell gets too big, the inward flow of nutrients and the outward flow of wastes
across that membrane will not be fast enough to keep the cell alive.
Observation of Cells: Microscopes
Microscopes allow us to study cells in detail. The ones that use visible light to illuminate objects are called
light microscopes. There are two types: Simple and Compound. A more powerful microscope is the
Electron microscopes use electrons instead of visible light to illuminate samples. Because electrons travel
in wavelengths that are much shorter than those of visible light, electron microscopes can resolve details
that are much smaller than you can see with light microscopes. Electron microscopes use magnetic fields
to focus beams of electrons onto a sample.

Limitations of Light Microscopy

Wavelengths of light are 400-750 nm
If a structure is less than one-half of a wavelength long, it will not be visible
Light microscopes can resolve objects down to about 200 nm in size

Electron Microscopy
Uses streams of accelerated electrons rather than light
Electrons are focused by magnets rather than glass lenses
Can resolve structures down to 0.5 nm
Cell Structure:

Bacterial cells (Prokaryote)

Cell Wall
Cell/Plasma membrane
Cytoplasm
Genetic Material(Nucleoid)
Ribosomes
Capsules
Flagella
Pili

Animal cells (Eukaryotes)

Plasma membrane
Nucleus
Ribosomes
Endoplasmic reticulum
Golgi body
Vesicles
Mitochondria
Cytoskeleton

Plant cells (Eukaryotes)

Plasma membrane
Nucleus
Ribosomes
Endoplasmic reticulum
Golgi body
Vesicles
Mitochondria
Cytoskeleton
Cell wall
Central vacuole
Chloroplast
Some of the organelles are common to all cells. These are;
Cell/Plasma Membrane
Genetic Material
Cytosol/Cytoplasm
Ribosomes

Structure common to all cells: Plasma Membrane

The plasma membrane is the boundary between the cell and its environment. It isolates the cell, regulates
what enters and leaves the cell. It allows interaction with other cells. It comprises of lipids and proteins.

Lipid Bilayer
Lipidsmainly phospholipidsmake up the bulk of
a cell membrane. A phospholipid consists of a
phosphate containing head and two fatty acid tails.
The polar head is hydrophilic, which means that it
interacts with water molecules. The nonpolar tails
are hydrophobic, so they do not interact with water
molecules, but they do interact with the tails of
other phospholipids. Lipid bilayers are the basic
structural and functional framework of all cell
membranes, gives membrane it's fluidity.

Fluid mosaic
Other molecules, including steroids and proteins, are embedded in or associated with the lipid bilayer of
every cell membrane. Most of these molecules move around the membrane more or less freely. A cell
membrane behaves like a two-dimensional liquid of mixed composition, so we describe it as a fluid
mosaic. The mosaic part of the name comes from a cell membranes mixed composition of lipids and
proteins. The fluidity occurs because the phospholipids in a cell membrane are not bonded to one another.
They stay organized as a bilayer as a result of collective hydrophobic and hydrophilic attractions.
Functions of different Membrane proteins:
Many types of proteins are associated with a cell membrane, and each type adds a specific function to it,
different cell membranes can have different characteristics depending on which proteins are associated
with them. For example, a plasma membrane has certain proteins that no internal cell membrane has.
Many plasma membrane proteins are enzymes. Others are adhesion proteins, which fasten cells together
in animal tissues. Recognition proteins function as identity tags for a cell type, individual, or species. Being
able to recognize self means that foreign cells (harmful ones, in particular) can also be recognized.
Receptor proteins bind to a particular substance outside of the cell, such as a hormone or toxin (Figure
4.8C). Binding triggers a change in the cells activities that may involve metabolism, movement, division,
or even cell death. Receptors for different types of substances occur on different cells, but all are critical
for homeostasis. Additional proteins occur on all cell membranes. Transport proteins move specific
substances across a membrane, typically by forming a channel through it. These proteins are important
because lipid bilayers are impermeable to most substances, including ions and polar molecules. Some
transport proteins are open channels through which a substance moves on its own across a membrane.

Functions of Cell membrane:

Membrane structure results in selective permeability

A cell must exchange materials

with its surroundings, a process
controlled by the plasma
membrane
Plasma membranes are
selectively permeable, regulating
the cells molecular traffic
Hydrophobic (nonpolar)
molecules, such as
hydrocarbons, can dissolve in the
lipid bilayer and pass through the
membrane rapidly
Polar molecules, such as sugars, do not cross the membrane easily. Cells must continuously receive
nutrients and rid themselves of waste productsone of the characteristics of life. Many of the proteins
that are associated with the plasma membrane are involved in moving molecules across the membrane.
Some proteins are capable of moving from one side of the plasma membrane to the other and shuttle
certain molecules across the membrane. Others extend from one side of the membrane to the other and
form channels through which substances can travel. Some of these channels operate like border
checkpoints, which open and close when circumstances dictate. Some molecules pass through the
membrane passively, whereas others are assisted by metabolic activities within the membrane.

Transport proteins
Transport proteins allow passage of hydrophilic substances across the membrane Some transport
proteins, called channel proteins, have a hydrophilic channel that certain molecules or ions can use as a
tunnel. Channel proteins called aquaporins facilitate the passage of water. Other transport proteins,
called carrier proteins, bind to molecules and change shape to shuttle them across the membrane. A
transport protein is specific for the substance it moves
Passive transport is diffusion of a substance across a membrane
Diffusion is the tendency for molecules to spread out evenly into the available space
Although each molecule moves randomly, diffusion of a population of molecules may be directional
At dynamic equilibrium, as many molecules cross the membrane in one direction as in the other
Substances diffuse down their concentration gradient, the region along which the density of a chemical
substance increases or decreases. No work must be done to move substances down the concentration
gradient.The diffusion of a substance across a biological membrane is passive transport because no
energy is expended by the cell to make it happen.

Structure common to all cells: Genetic Material

Eukaryotic Cell: Nucleus

The nucleus is the defining organelle of eukaryotic cells. The nucleus is separated from the cytoplasm by a
double membrane (two phospholipid bilayers); known as the nuclear envelope. The nuclear envelope
controls the passage of molecules between the nucleus and cytoplasm. The nucleus contains the DNA, the
stored genetic instructions of each cell. In addition, important reactions for interpreting the genetic
instructions occur in the nucleus.
In the nucleus, DNA is organized into discrete units called chromosomes
Each chromosome is composed of a single DNA molecule associated with proteins
The nucleolus is located within the nucleus and is the site of ribosomal RNA (rRNA) synthesis.

Components of Nucleus
Nuclear envelope
Nucleoplasm
Nucleolus
Chromosome
Chromatin

Functions of Nucleus
Keeps the DNA molecules of eukaryotic cells separated from metabolic machinery of cytoplasm
Makes it easier to organize DNA and to copy it before parent cells divide into daughter cells
Prokaryotic Cell: Nucleoid
The genetic material (DNA) in bacteria is packaged into a region of the cell called the nucleoid. The DNA
is not contained in a nuclear envelope and also it is naked that is, not associated with any proteins.
Bacteria also contain additional small circles of DNA called plasmids. Plasmids replicate independently and
may be passed from one cell to another.

Structure common to all cells: Ribosomes

Ribosomes are found in all prokaryotic and eukaryotic cells, where they synthesize proteins. Ribosomes
are non-membranous organelles responsible for the synthesis of proteins from amino acids. They are
composed of RNA and protein. Each ribosome is composed of two subunitsa large one and a small one.
As mentioned before, they are constructed in the Nucleolus. In eukaryotes, ribosomes carry out protein
synthesis in two locations
bound ribosomes: Many ribosomes are attached to the endoplasmic reticulum. Because ER that
has attached ribosomes appears rough when viewed through an electron microscope it is called
rough ER. Areas of rough ER are active sites of protein production.
free ribosomes: Many ribosomes are also found floating freely in the cytoplasm wherever
proteins are being assembled. Cells that are actively producing protein (e.g., liver cells) have
great numbers of free and attached ribosomes.

Structure common to all cells: Cytoplasm

In prokaryotes, cytoplasm inside the membrane is a jelly-like substance comprises of water, enzymes and
some proteins. It also contains the genetic material.In eukaryotes, the cytoplasm is a network of fibers
that organizes structures and activities in the cell and known as cytoskeleton
Between the nucleus and plasma membrane of all eukaryotic cells is a system of interconnected
protein filaments collectively called the cytoskeleton. The cytoskeleton is a network of fibers
extending throughout the cytoplasm. Elements of the cytoskeleton reinforce, organize, and move
cell structures, anchoring many organelles.
Cytomembrane System

The cytomembrane system is a series of interacting organelles between the nucleus and the plasma
membrane of eukaryotic cells. Its main function is to make lipids, enzymes, and proteins for secretion, or
for insertion into cell membranes. It also destroys toxins, recycles wastes, and has other specialized
functions. The systems components vary among different types of cells, but here we present the most
common ones:

Components of Cytomembrane System

Endoplasmic reticulum
Golgi bodies
Vesicles

Endoplasmic Reticulum

Part of the cytomembrane system is an extension of the nuclear envelope called endoplasmic reticulum, or
ER. ER forms a continuous compartment that folds into flattened sacs and tubes. The space inside the
compartment is the site where many new polypeptide chains are modified. Two kinds of ER, rough and
smooth, are named for their appearance in electron micrographs. Thousands of ribosomes are attached to
the outer surface of rough ER.

Rough ER
Arranged into flattened sacs
Ribosomes on surface give it a rough appearance
Some polypeptide chains enter rough ER and are
modified
Cells that specialize in secreting proteins have lots
of rough ER

Smooth ER
A series of interconnected tubules
No ribosomes on surface
Lipids assembled inside tubules
Smooth ER of liver inactivates wastes, drugs
Sarcoplasmic reticulum of muscle is a specialized
form that stores calcium

Functions of Smooth & Rough ER

The smooth ER
1. Synthesizes lipids
2. Metabolizes carbohydrates
3. Detoxifies drugs and poisons
4. Stores calcium ions
 The rough ER
1. Has bound ribosomes
2. Distributes transport vesicles, proteins surrounded by membranes
3. Is a membrane factory for the cell

Golgi Bodies
Golgi : The Golgi is a series of flattened membrane compartments, whose purpose is to process and
package proteins produced in-the rough endoplasmic reticulum. The processed molecules are packaged
into membrane vesicles, then targeted and transported to-their final destinations.
Functions of the Golgi apparatus
Modifies products of the ER
Manufactures certain macromolecules
Sorts and packages materials into transport vesicles

Vesicles
Small, membrane-enclosed, saclike vesicles form in great numbers, in a variety of types, either on their
own or by budding. There are many types but two main are:

i) Lysosomes: Digestion & recycling centers

i) Lysosomes that bud from Golgi bodies take part in intracellular digestion. They contain powerful
enzymes that can break down carbohydrates, proteins, nucleic acids, and lipids. Vesicles inside white
blood cells or amoebas deliver ingested bacteria, cell parts, and other debris to lysosomes for destruction.
The enzymes work best in the acidic environment inside the lysosome. Lysosomes break down worn out
cell parts or molecules so they can be used to build new cellular structures. Some types of cell can engulf
another cell by phagocytosis; this forms a food vacuole. A lysosome fuses with the food vacuole and
digests the molecules Lysosomes also use enzymes to recycle the cells own organelles and
macromolecules, a process called autophagy

ii) Peroxisomes: In plants and animals, vesicles called peroxisomes

form and divide on their own, so they are not part of the endomembrane system. Peroxisomes contain
enzymes that digest fatty acids and amino acids. They also break down hydrogen peroxide, a toxic by-
product of fatty acid metabolism. Peroxisome enzymes convert hydrogen peroxide to water and oxygen,
or use it in reactions that break down alcohol and other toxins.

Energy Related Organelles: Mitochondria & Chloroplast

Mitochondria:
The mitochondrion (plural, mitochondria) is a type of organelle that specializes in making ATP (molecule
used by cells as main energy source). They have various enzymes to catalyze cellular respiration. Bacteria
have no mitochondria; they make ATP in their cell walls and cytoplasm. Cells that have a very high
demand for energy tend to have many mitochondria e.g. liver needs more because needs more energy.
Mitochondria, like most organelles, can move within the cell and they grow and divide independently.
Each has two membranes, one highly folded inside the other. Double-membrane system: Smooth outer
membrane (lipid bilayer) faces cytoplasm and permeable to small solutes; blocks macromolecules wheras
Inner Membrane (cristae) folds back on itself to enlarge surface area for chemical reactions to take
place. Membranes form two distinct compartments. ATP-making machinery is embedded in the inner
mitochondrial membrane.

Mitochondria and chloroplasts have similarities with bacteria,

Enveloped by a double membrane
Contain free ribosomes and circular DNA molecules
Grow and reproduce somewhat independently in cells
They may have evolved from ancient bacteria that were engulfed but not digested. Mitochondria and
chloroplasts developed because as a prokaryote it gained protection by living inside the eukaryote and in
turn produced energy for the eukaryote (symbiotic relationship).
Chloroplasts: Capture of Light Energy
Plastids are a category of membrane-enclosed organelles that function in photosynthesis or storage in
plant and algal cells. Plastids called chloroplasts are organelles specialized for photosynthesis. Chloroplasts
contain the green pigment chlorophyll, as well as enzymes and other molecules that function in
photosynthesis. Chloroplasts are found in leaves and other green organs of plants and in algae.

Chloroplast structure includes

Stroma: Each has two outer
membranes enclosing a semifluid
interior, the stroma, that contains
enzymes and the chloroplasts own
DNA.
Thylakoids: Inside the stroma, a
third, highly folded membrane forms
a single, continuous compartment.
The folded membrane resembles
stacks of flattened disks. The stacks
are called grana (singular, granum).
Photosynthesis takes place at this
membrane, which is called the
thylakoid membrane. The abundance of chlorophylls in thylakoids is the reason most plants
are green. By the process of photosynthesis, chlorophylls and other molecules in the
thylakoid membrane harness the energy in sunlight to drive the synthesis of ATP. The ATP is
then used inside the stroma to build carbohydrates from carbon dioxide and water.

Locomotor appendages: Cilia & Flagella

Cilia Cilia (singular, cilium) are short, hairlike structures that project from the surface of some
cells.Mainly found in eukaryotic cells. Cilia are usually more profuse than flagella. The coordinated waving
of many cilia propels cells through fluid, and stirs fluid around stationary cells.

Flagella Flagella are long hair-like structure and present in both prokaryotic and eukaryotic cells.In
prokaryotes flagellum projects from the cell wall and enables a cell to move. In eukaryotes, flagellum
structure is different from the prokaryotic cells and whip back and forth to propel cells such as sperm
through fluid. They have a different internal structure and type of motion than flagella of bacteria.