Human HumaCount 30TS, 60TS - Service Manual PDF
Human HumaCount 30TS, 60TS - Service Manual PDF
Human HumaCount 30TS, 60TS - Service Manual PDF
| Service Manual
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REVISION LIST OF THE MANUAL
Rev. /DATE REVISION DESCRIPTION
01/2010-09 First edition
SYSTEM VERSION
COPYRIGHT
No part of this documentation may be reproduced in any form, nor processed, copied or
distributed by means of electronic systems, without prior permission of Human GmbH in
writing. Since all precautionary measures were taken into account in producing these operating
instructions, the manufacturer accepts no responsibility for any errors or omissions. This
includes any liability for damage that could arise from possible incorrect operation based on this
information. Subject to changes without notice as result of technical development.
TABLE OF CONTENTS
1 SAFETY INSTRUCTIONS 5
1.1 INTRODUCTION 5
1.2 USER WARRANTY 5
1.3 INTENDED USE OF THE INSTRUMENT 6
1.4 GENERAL SAFETY WARNINGS 6
1.5 DISPOSAL MANAGEMENT CONCEPT 7
1.6 INSTRUMENT DISINFECTION 7
1.7 BIOHAZARD WARNING 8
2 INTRODUCTION 11
2.1 NAME AND SERIAL NUMBER 11
2.2 INTEGRATED SOFTWARE 11
3 FUNCTIONAL DESCRIPTION 13
3.1 MAIN ELECTRONIC PARTS OF THE ANALYZERS 13
3.1.1 Counting chamber with electrodes and measuring aperture 15
3.1.2 HGB Measuring Head 16
3.1.3 Cell Counter Amplifier Board – HC30TS 16
3.1.4 Cell Counter Amplifier Board – HC60TS 17
3.1.5 MAIN CPU Board 19
3.1.6 Dimm-PC* Module 22
3.1.7 Opto sensors 22
3.1.8 Valve boards 23
3.1.9 TFT Display and START button Board 23
3.1.10 External Power Supply 24
6 ADJUSTMENTS 51
6.1 COMMON ADJUSTMENTS 51
6.1.1 Vertical movement, setting timing belt tension 51
6.1.2 Vertical opto sensor and needle settings 51
6.1.3 Setting the needle shaft 52
6.1.4 Setting the position of the chambers 53
6.2 HC30TS SPECIFIC ADJUSTMENTS 54
6.2.1 Setting the dilutor mechanics 54
6.2.2 Setting the horizontal movement 54
6.3 HC60TS SPECIFIC ADJUSTMENTS 55
6.3.1 Setting the dilutor mechanics 55
6.3.2 Setting the horizontal movement 55
CONTENTS
8 SERVICE OPERATION 67
8.1 POSSIBLE CAUSES OF NOISE 67
8.1.1 Contaminated reagent 67
8.1.2 Bad earth grounding 67
8.1.3 External electrical noise 68
8.1.4 Internal noise sources 68
9 MAINTENANCE 71
9.1 WEEKLY USER MAINTENANCE 71
9.1.1 Cleaning needle washing head 71
9.2 PERIODIC MAINTENANCE BY SERVICE 72
9.2.1 Check Self test and Device statistics 72
9.2.2 Cleaning and Greasing Dilutor Block 72
9.2.3 Checking and Lubricating Dilutor Piston Tips 72
9.2.4 Checking and Replacing Washing Head 73
9.2.5 Bleaching of Fluidic System 73
10 APPENDICES 75
10.1 WARNING FLAGS 75
10.2 USB B CONNECTOR COMMUNICATION 76
10.2.1 Characters and basic structure 77
10.2.2 Details of the 3.1 protocol 77
10.3 CABLING DIAGRAM 80
10.4 TUBING SCHEMATICS 82
10.5 RECOMMENDED KIT OF TOOLS 84
SAFETY INSTRUCTIONS 5
1 SAFETY INSTRUCTIONS
1.1 Introduction
This manual is considered as a part of the instrument; it has to be at the
operator’s hand as well as at the maintenance operator’s availability. For
accurate installation, use and maintenance, please read the following
instructions carefully. In order to avoid instrument damage or personal
injury, carefully read the ”GENERAL SAFETY WARNINGS”, describing the suitable
operating procedures. In case of breakdowns or any troubles with the
instrument, apply to the local Technical Service.
This warranty does not apply to damages incurred in shipment of goods. Any
damage so incurred shall be reported to the freight carrier for settlement or
claim.
supplied, the returning laboratory will be responsible for charges resulting from
non-acceptance of the instrument by the servicing centre, or from authority’s
interventions.
FIGURE 1
Biological Hazard
Symbol
Notes:
10
2 INTRODUCTION
Since HumaCount 30TS and HumaCount 60TS have so much common
characteristics, we issue a common Service Manual covering both instruments.
Information herein applies for all instruments unless otherwise noted.
To be well up in the instruments, please read this manual carefully to have the
knowledge for servicing the instruments perfectly and avoid extra costs and
wasting precious time.
In this manual, we are using the following conventions:
HC30TS – stands for HumaCount 30TS
HC60TS – stands for HumaCount 60TS
This HumaCount 30TS / HumaCount 60TS Service Manual contains the functional
descriptions of all analyzers, operation of the fluidic systems, adjustments and
settings, and very important information for the service personnel about the
service operations and possible problems.
3 FUNCTIONAL DESCRIPTION
FIGURE 2
Block diagram
Pump driver
USB A 1
FPGA Pressure sensor
XCS30XL
USB A 2
External keyboard Measure control
DIGIO Display
USB A 3 Assembly
HVB(50V, 150V)
USB A 4
USB stick
DC/DC ±12V
320*240 (QVGA)
USB DIMMPC TFT modul
HUB AMD Elan SC-520 Start
PIC24 w/ touchscreen
ȝController To PIC ADC button & w/ LED backlight
Status
LED board
USB B
LCD backlight driver
Sign Collection Board
External PC
BLTS v1.0
Touchscreen
interface
4
FIGURE 3
1 Measuring chamber
2
1 Washing inlet
2 Counting chamber
3
3 Opening for measuring tube
5
4 Chamber extender
5 Platinum electrode
6
6 Draining connection
FIGURE 4
Measuring tube
6
1 Complete measuring tube
5 2 O-rings
3 Reference electrode
4 U-shaped metal fixing
4 5 Aperture
6 Measuring tube with aper-
ture (70/80/100μm)
2 2 3
16
FIGURE 5 2
1 3
1 LED
2 Connection to
the amplifier
3 TSL 235
The analyzer performs enhanced hemoglobin measurement technology for HGB
measurement. The frequency output signal of TSL235 is counted by a digital
counter in the FPGA circuit.
This counter counts up while the LED is on and counts down while the LED
is off. The LED and direction of counting are switched with a 100 Hz signal.
This method provides “real time backlight correction”, which makes the HGB
FIGURE 6
Cell counter amplifier board
2
1 Connection to HVB on Main
Amplifier board includes a 3-stage main amplifier channel, which gains input
signal to the 0...3.3 V range (this is the input range of the A/D converter, which
is placed on the MAIN board). The RSW signal changes the gain (RBC, WBC) in
the feedback of the second amplifier stage with U2 (MAX319) analog switch.
Amplifier gain and offset are adjusted by software.
DHON signal switches on the LED and the MVON signal – which is active during
counting – switches off the Photo Detector in the HGB head, to prevent noise
generated by the HGB detector.
The other side of the amplifier board contains special connectors for the cham-
ber and the HGB head (JP4).
FIGURE 7
Cell counter amplifier board - 3
front side 1
FIGURE 8
Cell counter amplifier board -
back side
Amplifier board includes a 3-stage main amplifier channel, which gains input
signal to the 0...3.3 V range (this is the input range of the A/D converter on the
Main board). The RSW signal (with Q8 transistor) changes the input electrode
through REL2 relay.
The bottom side of the amplifier board contains special connectors for the
electrodes and the HGB head (JP2).
DHON signal - from the MAIN board - switches on (with Q4) the LED and the
PLS signal switches off the Photo Detector in the HGB head, to prevent noise
generated by the HGB detector.
MAIN board is responsible to control the instrument: contains the main power
regulator circuits, valve and motor driver circuits and other connections for the
fluidic and pneumatic system’s parts, responsible for the specific measurement
processing functions.
The central micro-controller with a FPGA and with several other digital chips
(buffers, decoder, multiplexer) handles the pneumatic system, displaying,
measurement and data management.
Power system: filtering the +12V Input and generates +3.3V (FPGA), +5V (Digital
power), +7.5V (Printer power). Filtered +12V is used for the power of motors and
valves.
Motor drivers: 4 power drivers; Horizontal, Vertical/Sample rotor motors and
dilutor motors (2 in HC60TS) have separated ribbon cable connections.
Valve driver: consists two 8-bit, powered output shift registers (with built in
protection diodes) and there is one common ribbon cable connection for the
valve boards. The peristaltic pump has a separated power FET driver circuit for
more reliable operation.
Measurement processing: the A/D conversion made by the micro-
controller itself, but several preprocessing steps (time limits, noise handling,
pulse integration) taken by the external analog circuitry.
20
15 14
1
FIGURE 9
Main board - front view
2
1 Power Supply 13
ster
3 USB HUB 4
4 DIMMPC
5
5 RS232/USB Converter
11
6 Speaker
7 FPGA
6
8 Power Supply 12VDC-
>5VDC, 3.3VDC 7
10
9 High Voltage Circuit 12VDC
-> ±12VDC, 50VDC for
measurement, 150VDC for
8
cleaning
10 Microcontroller
11 TFT connection
12 TFT backlight driver
13 Motor drivers
9
14 Valve drivers
15 Pump drivers
14 13 12 11
FIGURE 10
1 Main board - rear view
1 Pressure Sensor
3 10 2 Motor Connectors
3 Front Panel USB Connector
4 Connector to TFT, Backlight
FIGURE 11
DIMM-PC 1
10
1 Flash BIOS
2
2 32 Mbytes RAM 9
3 CMOS EEPROM
4 On-board SMPS
5 Edge connector 3 8
6 AMD Elan SC520 CPU
7 Super I/O
8 Realtime clock 4
9 SSD controller 7
10 SSD
FIGURE 12
Opto sensor
FIGURE 13
Valve assembly
1
1 Valves
2 Valve Board
3 Connection to Main board
HC30TS has 5 valves, while HC60TS has 6 valves in Valve board 6-12 module.
The valve boards are connected to controller and driver chips which are located
on the MAIN board.
FIGURE 14
Front panel connections
1 Touchscreen connector
2 Start button & status LED
4
connector
3 Ribbon cable from TFT/
Touch board to Main board
4 TFT connector to Main board
5 TFT Backlight connector
1 5 6 Ribbon cable from TFT/
Touch board to Main board
6
2
3
24
FIGURE 15
Power supply
1 230 AC inlet
2 12V DC outlet
FIGURE 16 :
V2 DilWash
1
3
V4 DilNeedle
1
2 1
V3 DilChamber
HC 30TS fluidic schematic
3
3
2
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure V1 Cleaner
Meter 3
P V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
1
Lyse WBC
Macro
3
V8 Bubble
2
Micro dilutor
Dil M3
HGB
2
LYSE V7 DrainAperture
M2
1
Ver
2
V6 DrainChamber
2 1
1
Pump
2 1 1 2 3-way
M1 Stepper Motor 2-way Valve Valve
Closed = Off 3
1-3 = Off
WASTE Open = On 2-3 = On
26
V2 DilNeedle V1 DilWash
1
2 1
FIGURE 17
3
3
2
HC 60TS fluidic schematic
1
V4 DilChamber
2
P DILUENT
V3 Cleaner
Puffer 2 1
Reservoir
V5 LyseWBC 3
V10
1 2
1 2
3 3
1 2
CLEANER
Diluent Diluent Lyse V7 Aperture
1
MIX
RBC
WBC
3
V9
2
Bubble
Diluent
2
2
V6 DrainChamber V8 Drainchamber
2
1
1
1
V11 DrainPuffer
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2
3-way
Stepper Motor 2-way Valve Valve
M1
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
FIGURE 18
Sample probe motors
1 Vertical motor 1
2 Sample/Horizontal motor
(not visible)
FIGURE 19
Needle washing head
1 1 Clean diluent
2 2 Pump to waste
FIGURE 20
Puffer reservoir
28
FIGURE 21
4
1 Micro dilutor
2 Positioners 1 5
3 Positioners 6
4 Pistons
5 Pistons
6 Diluent syringe
7 Lyse syringe
2
FIGURE 22
1 Micro dilutor
2 Diluent 1 syringe
3 Positioners
4 Positioners
5 Lyse syringe
6 Diluent 2 syringes
1 5
2 6
In HC60TS this unit includes two dilutor channels – one for diluent and sampling,
and another one for lyse and diluent reagents. There are two stepper motors,
4 opto sensors, five syringes and piston rods with gear transmission. The Micro
dilutor syringe makes the aspirating while the motor moves down. The syringes
are mechanically connected with a loose mechanism, so there is a phase along
the track, where the micro dilutor doesn’t move.
30
FIGURE 23
FIGURE 24
4.1.9 PUMP
Pump generates regulated vacuum and drains the fluidic system. It is connected
to the MAIN board and it has its own driver circuit (Power FET). In HC60TS there
are two pumps.
The pump is maintenance free.
FIGURE 25
FIGURE 26
1 TFT display
1
2 Start button
3 USB socket
4 Sample holder/Sample rotor
3
32
FIGURE 27
1 Cover screws
2 Cover screws
1 2
FIGURE 28
1 3
4
5
1 Cover screw
2 Reagent inlets
3 cover screw 6
4 Power switch 7
5 Cover screw
8
6 USB A inlet
7 USB B inlet
8 Cover Screw 9
10
9 Grounding screw
10 12 VDC power IN
11 Cover screw
2
11
FIGURE 29
6
1 Main board
1
2 Pump II (only in HC60TS)
7
3 Vertical motor
2
4 Pump I
5 Amplifier box
6 Valves
7 Dilutor motor
3
8 Valves
9 Sample/Horizontal motor
8
5 9
FIGURE 30
1
1 Valves 1-5
2 Micro dilutor
6
3 Dilutor 1
2 7 4 RBC Chamber
3 8 5 MIX/WBC Chamber (only in
HC60TS)
6 Puffer reservoir
4
7 Lyse syrenge
5
8 Dilutor 2
9 Valves 6-10 (6-11 in HC60TS)
9
34
FIGURE 31
Construction side of
HC30TS
1 MAIN board 1
2 Dimm-PC
FIGURE 32
Construction side of
HC60TS
1
1 MAIN board
2 Dimm-PC
2
In the detailed process description figures, the active tube is filled with black
color, while an arrow shows the direction of the flow. Moving mechanic parts
have another arrow indicating direction of movement. Only opened (On) valves
are mentioned in this section while all the other valves are closed (Off).
HC30TS / HC60TS employs software counters to estimate waste (and other re-
agent) level. Software integrates volume of the reagents used, and gives a mes-
sage when this volume reaches the preset tank capacity.
FIGURE 33
Chamber draining
Sampling process
Needle washing
Diluting process
(1:160)
Sampling process
(from primary
dilution)
Needle washing
Lysing process
FIGURE 34
Generating
measuring Fill up with 1ml
vacuum diluent for RBC
dilution
WBC counting
process
Dilution process
(1:25600)
HGB
measurement
Needle washing
Extensive cleaning
Generating
measuring
vacuum
Blank HGB
measurement
RBC
measurement
Draining
END
38
FIGURE 35 V2 DilWash
1
3
V4 DilNeedle
2
1
2 1
V3 DilChamber
2
3
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure V1 Cleaner
Meter 3
P V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
1
Lyse WBC
Macro
3
V8 Bubble
2
Micro dilutor
Dil M3
HGB 2
LYSE V7 DrainAperture
M2
1
Ver
2
V6 DrainChamber
2 1
1
Sample/
V10 DrainPuffer
M1 H motor
Pump
2 1 1 2 3-way
Stepper Motor 2-way Valve Valve
M1
Closed = Off 3
1-3 = Off
WASTE Open = On 2-3 = On
There is also another sampling process for the second (RBC) dilution, 25 μL of
primary dilution is aspirated by the sampling needle from the chamber but it
is kept in the sampling needle during the WBC measurement and the cleaning
process.
FIGURE 36
V2 DilWash
1
3
V4 DilNeedle
1
2 1
V3 DilChamber
3
3
2
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure V1 Cleaner
Meter P 3
V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
1
Lyse WBC
Macro
3
V8 Bubble
2
Micro dilutor
Dil M3
HGB
2
LYSE V7 DrainAperture
M2
1
Ver
2
V6 DrainChamber
2 1
1
Pump
2 1 1 2 3-way
M1 Stepper Motor 2-way Valve Valve
Closed = Off 3
1-3 = Off
WASTE Open = On 2-3 = On
The Macro syringe pushes the diluent through V4 (Off), V3 (Off), V2 (On). The
Pump aspirates the diluent from the washing head through V10(On), while the
M2 Vertical motor moves the sampling needle up.
40
FIGURE 37
V2 DilWash
1
3
V4 DilNeedle
2
1
2 1
V3 DilChamber
3
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure V1 Cleaner
Meter 3
P V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
1
WBC
Macro Lyse
3
LYSE V7 DrainAperture
M2
1
Ver
2
V6 DrainChamber
2 1
1
Sample/
V10 DrainPuffer
M1 H motor
Pump
2 1 1 2 3-way
Stepper Motor 2-way Valve
M1 Valve
Closed = Off 3
WASTE 1-3 = Off
Open = On
2-3 = On
FIGURE 38
V2 DilWash
1
3
V4 DilNeedle
1
2
2 1
V3 DilChamber
3
3
2
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure V1 Cleaner
Meter P 3
V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
1
Lyse WBC
Macro
3
V8 Bubble
2
Micro dilutor
Dil M3
HGB
2
LYSE V7 DrainAperture
M2
1
Ver
2
V6 DrainChamber
2 1
1
Pump
2 1 1 2
3-way
M1 Stepper Motor 2-way Valve Valve
Closed = Off 3
1-3 = Off
WASTE Open = On 2-3 = On
For better mixing the macro syringe pushes some air bubbles (aspirated through
the washing inlet of the chamber and V8) after the lysing process through V4
(Off), V3 (On), V9 (Off) V8 (On).
42
FIGURE 39
V2 DilWash
1
3
V4 DilNeedle
2
2 1
V3 DilChamber
3
3
2
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure V1 Cleaner
Meter P 3
V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
1
Lyse WBC
Macro
3
V8 Bubble
2
Micro dilutor
Dil M3
HGB
2
LYSE V7 DrainAperture
1 M2
Ver
2
V6 DrainChamber
2 1
1
Pump
2 1 1 2
3-way
M1 Stepper Motor 2-way Valve Valve
Closed = Off 3
1-3 = Off
WASTE Open = On 2-3 = On
1
3
V4 DilNeedle
1
2
2 1
V3 DilChamber
3
3
2
V5 LyseWbc DILUENT
1 2
3 2 1
Pressure V1 Cleaner
Meter P 3
V9 DilAperture
1 2
CLEANER
Puffer
RBC
Reservoir
1
Lyse WBC
Macro
3
V8 Bubble
2
Micro dilutor
Dil M3
HGB
2
LYSE V7 DrainAperture
M2
1
Ver
2
V6 DrainChamber
2 1
1
Pump
2 1 1 2
3-way
M1 Stepper Motor 2-way Valve Valve
Closed = Off 3
1-3 = Off
WASTE Open = On 2-3 = On
FIGURE 41
MIX/WBC Needle
RBC chamber Both chambers
chamber subsystem
measurement
HGB Blank
measurement
Sampling process
(from primary
dilution)
Generating
measuring
vacuum
WBC counting
process, HGB
measurement
RBC counting
process
Draining
Cleaning
Fast Blank
measurement
END
FIGURE 42
V2 DilNeedle V1 DilWash
1
2 1
3
3
2
1
V4 DilChamber
3
P V3 Cleaner
DILUENT
Puffer 2 1
Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3
1 2
CLEANER
Diluent Diluent Lyse V7 Aperture
1
MIX
RBC
WBC
3
V9
2
Bubble
Diluent
V6 DrainChamber V8 Drainchamber
2
1
1
V11 DrainPuffer
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2
3-way
Stepper Motor 2-way Valve Valve
M1
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
FIGURE 43
V2 DilNeedle V1 DilWash
1
2 1
3
3
2
1
V4 DilChamber
3
P DILUENT
V3 Cleaner
Puffer 2 1
Reservoir
V5 LyseWBC 3
V10
1 2
1 2
3 3
1 2
CLEANER
Diluent Diluent Lyse V7 Aperture
1
MIX
RBC
WBC
3
V9
2
Bubble
Diluent
V6 DrainChamber V8 Drainchamber
2
1
1
V11 DrainPuffer
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2 3-way
Stepper Motor 2-way Valve Valve
M1
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
The second sample – 25 μL of primary dilution – is made right after the first
dilution. The second dilution is made in the RBC chamber.
V2 DilNeedle V1 DilWash
1
2 1
3
3
2
1
V4 DilChamber
3
P DILUENT
V3 Cleaner
Puffer 2 1
Reservoir
V5 LyseWBC 3
V10
1 2
1 2
3 3
1 2
CLEANER
Diluent Diluent Lyse V7 Aperture
1
MIX
RBC
WBC
3
V9
2
Bubble
Diluent
V6 DrainChamber V8 Drainchamber
2
1
1
V11 DrainPuffer
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2 3-way
Stepper Motor 2-way Valve Valve
M1
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
For better mixing the macro syringe pushes some air bubbles (aspirated through
the washing inlet of the chamber and V9) after the lysing process through V4
(Off), V10 (On), V9 (Off).
48
FIGURE 45
V2 DilNeedle V1 DilWash
1
2 1
3
3
2
1
V4 DilChamber
2
P DILUENT
V3 Cleaner
Puffer 2 1
Reservoir
V5 LyseWBC 3 V10
1 2
1 2
3 3
1 2
CLEANER
Diluent Diluent Lyse V7 Aperture
1
MIX
RBC
WBC
3
V9
2
Bubble
Diluent
V6 DrainChamber V8 Drainchamber
2
1
1
1
V11 DrainPuffer
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2 3-way
Stepper Motor 2-way Valve Valve
M1
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
FIGURE 46
V2 DilNeedle V1 DilWash
1
2 1
3
3
2
1
V4 DilChamber
3
P DILUENT
V3 Cleaner
Puffer 2 1
Reservoir
V5 LyseWBC 3
V10
1 2
1 2
3 3
1 2
CLEANER
Diluent Diluent Lyse V7 Aperture
1
MIX
RBC
WBC
3
V9
2
Bubble
Diluent
V6 DrainChamber V8 Drainchamber
2
1
1
V11 DrainPuffer
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2 3-way
Stepper Motor 2-way Valve Valve
M1
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
50
FIGURE 47
V2 DilNeedle V1 DilWash
1
2 1
3
3
2
1
V4 DilChamber
2
P DILUENT
V3 Cleaner
Puffer 2 1
Reservoir
V5 LyseWBC 3
V10
1 2
1 2
3 3
1 2
CLEANER
Diluent Diluent Lyse V7 Aperture
1
MIX
RBC
WBC
3
V9
2
Bubble
Diluent
2
V6 DrainChamber V8 Drainchamber
2
1
1
1
V11 DrainPuffer
Sample/
M1 H motor
Pump 1
Pump 2
2 1 1 2 3-way
Stepper Motor 2-way Valve Valve
M1
Closed = Off 3
1-3 = Off
Open = On 2-3 = On
WASTE
After that the Diluent 2 syringes pushes the cleaner reagent remaining in the
tube between V10 and V3 into the chamber.
6 ADJUSTMENTS
The adjustments below are made in the factory. Readjustment of following
parts is necessary if some components are replaced.
FIGURE 48
FIGURE 49
2 1 opto sensor
2 flag
The flag (1) mounted on the vertical needle moving mechanism must run freely
between the two parts (2) of the opto-sensor.
52
The vertical position can be set by loosening the two mounting screws of the
opto sensor and moving it up or down. In the correct setting the end of the
needle is coplanar of the lower plane of needle cleaner unit. The opto sensor
state could be checked in the software (see chapter 8.2.4)
FIGURE 50
FIGURE 51
FIGURE 52
54
FIGURE 53
FIGURE 54
FIGURE 55
FIGURE 56
56
You can access the service calibration menu logged in as Service User:
Maintenance-->Calibration-->Service.
Press white data field to modify calibration factor. A numeric input screen will
show up so that you can enter values.
All values must be in the 0.8…1.2 range.
Press Accept to proceed with new settings, or Cancel to keep values unchanged.
History: You can check the previous calibration factors with the date of change
in a table form.
Apply user calibration factors function is used to combine user and service
calibration factors. The software will multiply the existing factors, and move
them to the Service level to set user factors to 1.00.
7.2.2 TROUBLESHOOTING
With Troubleshooting options provide tools to test mechanical components.
From the Motor Test submenu the service person could run each or all motor
tests.
You can check the offset on the amplifier board. The current offset and the
acceptance range are displayed.
7.2.3 STRESS
In Stress mode, the instrument performs measuring cycles without sample
(blank measurements) continuously. This can be used for burn-in tests, or to
check pneumatic system after changing any main fluidic parts.
You can have information about stability, cleanliness, HGB operation, and
counting time stability. Results of the PLT and HGB blank results are displayed
in table format.
64
You can detect any kind of noise, or bubbles in the system if the PLT is not stable
low, or HGB has big variation. To exit from this mode press the Abort button (at
the end of a normal cycle) until the Stress operation is finished.
If you are logged in as different user touch Logout(USERNAME) and then the Log
In button, else touch Log In button.
At Log In screen Press the START button on front panel, so the Login Name will
change to Service.
Touch Password empty field.
Type in the Service User password which is 6484, then touch Enter button.
Touch Accept button on Log In screen.
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8 SERVICE OPERATION
inductive noise will be coupled into the instrument. It is better to create a real
earth grounding and connect it to the rear screw.
8.1.4.4 Pneumatic failures, liquid paths that conduct noise into the chamber:
- Liquid remains under the chamber in drain tube (during measurement the
conducting liquid remains inside the drain tube making noise to appear
there).
- Liquid remains in the washing inlet at top of the chamber (during measure-
ment the conducting liquid remains inside the chamber wash tube making
noise to appear). The software is not compatible with the mechanics, or rela-
ted valve is bad/partly clogged, or the tubing is clogged/loose.
- Lyse path guides in noise (during counting, if the a liquid in the draining tube
is touching lyse reagent in T-fitting, noise can appear). Check the lyse path,
and the lyse valve as well.
9 MAINTENANCE
FIGURE 57
HumaCount 60 TS chambers
1 Washing head
2 Measuring chambers
3 Measuring apertures
2
3
72
FIGURE 58
HumaCount 30 TS chamber
1 Wash head
2 Measuring chamber
1. Exit Measure menu. Open the side door after the needle has stopped moving.
2. Gently rub the lower surface of the washing head with a damp cloth or wiper
to remove the salt build-up.
3. Close the side door.
Repeat this step for lyse and dilutor pistons as well. Check the condition of the
micro piston sealing, and replace if necessary.
10 APPENDICES
transmission. For a more detailed discussion on the meanings of the various parameters and
histograms, please refer to the instruments’ user manuals.
header1 to header8 are the lab header lines these lines are
TABLE 5
header1 to header 8 defined by the user in the instrument settings any or all of
these lines can be empty
Serial No.:<HT>serial serial is the 6 digit serial number of the instrument
RecNo:<HT>recno recno is the internal record number, at most 6 digits
Sample ID:<HT>sampleid sampleid is at most 8 characters long
Patient ID:<HT>patientid patientid is at most 20 characters long
Patient Name:<HT>patientname patinetname is at most 32 characters long
Mode:<HT>mode
Doctor:<HT>doctor doctor is at maximum 16 characters long
value is a number of at most 3 digits, unit is either „years” or
Age:<HT>value<HT>unit
„months”
Birth(ymd):<HT>birthdate birthdate is an 8 digit number, format: yyyymmdd
gender is „Male”, „Female”, „Neutered”, „Spayed” or a single
Sex:<HT>gender
„-” character
Test date(ymd):<HT>date date is an 8 digit number, format: yyyymmdd
Test time(hm):<HT>time time is a 6 digit number, format: hhmmss
Param<HT>Flags<HT>Value<HT>
this is a header line, always the same
Unit<HT>[min-max]
There are 24 similar lines:
param is the parameter name, at most four characters long,
possible values are (in sequence): WBC, RBC, HGB, HCT, MCV,
MCH, MCHC, PLT, PCT, MPV, PDWs, PDWc, RDWs, RDWc,
LYM, MON, NEU, LY%, MO%, NE%, EOS, EO%, BAS, BA%
flag is a single character indicator, can be „ ” (space), „+”, „-”,
„E” and „*”(asterisk) value is the measured parameter value,
param<HT>flag<HT>value<HT>
exactly 4 characters: number with a possible decimal dot,
unit<HT>[min-max]
padded with spaces on the left side, or 4 minus signs „----”,
or 4 spaces „ ” unit is at most 4 characters long, possible
values are „109/L”, „103/μL”, „1012/L”, „106/uL”, „fL”, „%”,
„g/L”, „g/dL”, „mmol/L”, „pg”, „fmol”, depending on the
parameter. Min and max are the lower and upper bounds of
the normal range, exactly 4 characters, including a possible
decimal dot, padded with spaces on the left side
flags is a series of characters indicating errors, at most 32
Flags:<HT>flags
characters long, upper or lowercase letters „a” to „z”
always the same, indicates the beginning of the WBC histo-
WBC graph
gram
wbcscale is maximum 3 digit number, indicating the fl value
Scale(fl):<HT>wbcscale
of the last channel, value is usually 400
FIGURE 59
HC30TS cabling diagram
FIGURE 60
HC60TS cabling scheme
82
V1 V2 V3 V4 V5
2 2 2 2 2
3 3 3 3
1 1 1 1 1
M D L
70u
Dilutor
11 1 1 1 1
3 3 3
2 2 2 2 2
V6 V7 V8 V9 V10
out in
Pump
D
V1 V2 V3 VV4
12 V5
MAIN Board FIGURE 62
Pressure
Tubing schematics HC60TS
2 2 2 2 2
3
1
3 1 1
3
1
3
1 Sensor
M D1 D2 L
D2
MEA
PUFFER
W W
II.
out in
2 1
3
2 1
3
1
3
1
3
Pump
1 2 1 2 2 2
V6 V7 V6 V9 V10 V11
out I. in
Pump
84